OBJECTIVE:To provide reference for the establishment of coordinative mechanism among different interest groups during the implementation of essential drug system. METHODS:Based on game theory,game behaviors of various interest groups were studied with public medical institution as study core. Behavior relationships among various interest groups were ensured to find out equilibrium point of interests. RESULTS & CONCLUSIONS:In the implementation process of National Essential Drug System,great importance should be attached to the study on game phenomena and behavior among various interest groups to make sure implementation of National Essential Drug System.

ObjectiveTo study the effect of electromygraphic biofeedback on upper extremity function in patients with hemiplegia after stroke.Methods79 patients were randomly divided into experimental group(40 cases) and control group(39 cases).Patients in each group were given clinical treatment and regularly physical therapy, while those in experimental group were given electromygraphic biofeedback training. All patients were evaluated with electromyography amplitude and active range of motion(AROM) of wrist dorsiflexion and upper extremity Fugl-Meyer assessment pre-treatment and 3 months after stroke.ResultsAll the patients gained improvement after treating(P<0.05).Compared with the controls, patients in experimental group significantly improved their function in all measured item(P<0.01).ConclusionThe electromygraphic biofeedback can improve the arm function of hemiplegic patients.

Objective To investigate the neuroprotective effect of ginkgolide B (BN52021) o n the histopathology of brain tissue after traumatic brain injury in rats. Methods Forty-eight healthy SD rats, weighing 250 g, were evenly randomized into 4 groups: sham control group, model group, low dose BN52021 group and high dose BN52021 group. Rats in the latter 3 groups were made into fluid percussion brain injury models. After operation, the rats in the low and high dose BN52021 groups were treated with BN52021 (low dose: 1 mg/kg, ip, high dose: 5 mg/kg, ip, once daily for 7 days). On the 7th day after treatment, cerebral tissues were harvested from each group, and the histopathological changes of brain tissue were observed by Fast blue, electron microscope and immunohistochemical method. Results Compared with sham control group, model group had significantly decreased neurons (P<0. 05), increased OX-42 immunoreactive microglial cells and astrocytes (P<0. 05), and cells positive for caspase-3 (P<0. 05). Electron microscope found chromatin aggregation, nuclear fragmentation, rounder and larger mitochondria, void formation and disappeared cristae of mitochondria, endoplasmic reticulum hypertelorism, increased lysosomes, and nuclear membrane folding. Compared with model group, the low and high dose BN52021 groups had significantly decreased proportions of microglial cells and astrocytes (P<0. 05), significantly decreased caspase-3 positive cells (P<0. 05), and improved ultrastructure, with the improvement in the high dose group being more notable than that in the low dose group. Conclusion BN52021 has protective effect on the morphology of brain tissue in rats with traumatic brain injury.

China ; Health Status ; Humans ; Occupational Health ; Shoes

By means of a comprehensive coverage of the numbers,distribution,makeup and efficiency of health human resources in China,a comparative study was made as to the deployment for the present stage.It reveals the following findings:sufficient human resources in general,with its growth lagging behind economic development; out-of-balance deployment; irrational makeup as shown in the inverse ratio between doctors and nurses; good efficiency of the health professionals.Based on these findings,the researchers put forward the feasible policy suggestions as to optimizing the mechanisms of the introduction,training,evaluation and incentives for these professionals.

Objective To investigate the infection of Chlamydia (CT) Untie urea mycoplasma (UU) and person type mycoplasma (MH) in urology outpatients clinic and sexually transmitted diseases clinic and drug resist-ance. Methods A British UNIPATH Clean View Chlamydia rapid immunoassay kits and Tianyang Zhongshan City electronic biosensor Limited production of mycoplasma culture and identification of susceptibility kit were used for the detection of secretion from 3,280 cases of non-gonococcal urethritis (GNU). Results Of 3,280 cases of GNU, only 241 cases were detected with positive CT, accounting for 7.35%, 1163 cases with simply UU positive, ac-counting for 35.46%, only 14 cases with MH positive, accounting for 0.43% ,. Overall detection rate of UU and CT was 42.77% and 11.59% respectively. 122 cases were detected with positive UU + MH, accounting for 3.72%. Female infection rate was higher than that of males (P < 0.01 ) ; UU, MH, MH + UU were more sensitive to azithremycin, Josamycin, doxycycline, roxithromycin, Minocycline. Conclusion The infection rate of urogenital mycoplasma and ehlamydia is higher and drug resistance rate is different. Azithromycin and Josamycin are the best to treat mycoplasma infection, followed by doxycycline, Pyronine Doxycycline, Minocycline.

Objective:To observe the effect of Fufang Congrong Yizhi Capsule on myristoylated alanine-rich C-kinase substrate (MARCKS) mRNA level in hippocampus of old dementia rats induced by ?-amyloid(1-40). Methods: Animals were divided randomly into young group, old group, sham operation group, model group, western medicine group and Chinese medicine group. Model, western and Chinese medicine groups received ?-amyloid (1-40) intracerebroventrical injection to build dementia animal model, and then Chinese and westein medicine groups received Fufang Congrong Yizhi Capsule and donepezil for 14 days, respectively. To observe changes of behavior and MARCKS mRNA under the effect of Chinese and western medicine by Morris test and RT-PCR test. Results: Learning and memory function of Chinese and western medicine groups were better than that of model group. MARCKS mRNA of model group was increased significantly, that of Chinese medicine group was decreased compared with model group. Conclusion: Fufang Congrong Yizhi Capsule can reduce the level of MARCKS mRNA in hippocampus of old dementia rats caused by ?-amyloid(1-40), through which it can improve the learning and memory function of the dementia old rats.

Doctors of past dynasties already understood deficiency of kidney essence,phlegm and blood stasis were important for dementia.As the increasing recognition about Alzheimer disease and the offer of 'toxin damaging brain vessel' hypothesis,we thought ?-Amyloid,the pathology of Alzheimer disease,had the character of damaging brain and marrow,so it belonged to internal toxin,internal toxin damaging brain and marrow was also the pathology of the disease.Deficiency of kidney essence was the precondition,insufficient brain and marrow and internal toxin damaging brain and marrow were the two causes of brain and marrow consumption and spirit dysfunction,which were the pathology of Alzheimer disease in Chinese medicine.

Objective To investigate the protective effect of adiponectin on angiotesin Ⅱ (Ang Ⅱ)-mediated cardiomyocyte apoptosis,and explore the related mechanism. Methods Cardiomyocytes isolated from neonatal rats were cultured in vitro. The identity of cardiomyocytes was confirmed by morphological examination and staining with anti-sarcomeric ?-actin antibody,and most (more than 95%) of the cells were identified as neonatal rat ventricular myocytes. After being placed in a serum-free medium for 24 hours,the ventricular myocytes were randomly grouped and received the following treatments respectively:placebo,or Ang Ⅱ (1?mol/L),or Ang Ⅱ (1?mmol/L) plus adiponectin (30mg/L). The protein levels of Bcl-2 and Bax were detected by Western blotting. The apoptotic rate and the levels of intracellular reactive oxygen species (ROS) in the ventricular myocytes were determined by flow cytometry. The survival rate of ventricular myocytes was evaluated by trypan blue staining. Results As compared with placebo group,the apoptosis of neonatal rat ventricular myocytes was significantly promoted in 1?mol/L angiotesin Ⅱ group,the survival rate of cardiomyocytes was significantly reduced,with the protein expression of Bax and the ROS level increased,and the protein expression of Bcl-2 decreased (P

In type 2 diabetes mellitus,the levels of leptin were higher in female and obese subjects compared with male and non-obese subjects.There was negative relationship in female between leptin and HbA_1c,and negative relationship between leptin and HDL-C in male.Gender and obesity had no effect on TNF-? and NP Y.