Objective:To evaluate the clinical efficacy of telmisartan in patients with mild to moderate hypertension by observing the 24 hour mean blood pressure before and after treatment respectively.Methods:Twenty-six patients with mild to moderate hypertension took telmisartan 80mg once daily.After the treatment of telmisartan,ambulatory blood pressure monitoring (ABPM) was done.Results:After administration of telmisartan,24 hour mean blood pressure,blood pressure in the day and night decreased(P

Objective: To study the lung tissue TLR4 expression and airway hyperresponsiveness(AHR) in the mice infected by respiratory syncytial virus(RSV).Methods:16 clean,6 weeks old BALB/c mice were divided into two groups equally(RSV group and control).One week after RSV infection,AHR was detected by inhaling the increased concentration of methacholine,TLR4 mRNA expression was measured by RT-PCR methods,and the correlation between AHR and TLR4 expression was also analyzed.Results:An important difference was detected in two groups' airway resistance when the inhaled concentration of methacholine was 3.2g/L(P

Objective:To investigate the association of a(TA)n dinucleotide repeat polymorphism with unknown aetiology hypomenorrhea.Methods:100 normal menstruation person(control group) and 100 hypomenorrhea patients(case group)were recruited.they all came from southen-western China.The(TA)n dinucleotide repeat in ERa gene upstream hypervariable region were purifed,cloned and sequence analysed.we observe the ERa gene(TA)n dinucleotide repeat polymorphism distribution in the two groups.Results:All samples exhibted 7 different alleles.The(TA)n dinucleotide repeats were 11~17,The most prevalent allele was TA14 repeat in control group.The distribution of the different alleles of the(TA)n dinucleotide repeat sequence in the group and control group has significant difference(?2=13.412,P =0.037).Fisher's exact test was applied to compare the frequency of each a1lele between two groups.The frenquency of the TA13 allele was found to be significantly greater in hypomenorrhea group(P =0.006),and the frenquency of the TA15 allele was less in the group(P =0.033).Conclusion:A(TA)n dinucleotide repeat sequence which is in ERa gene upstream hypervariable region was associated with unknown aetiology hypomenorrhea.The TA13 allele was found to be associated with unknown aetiology hypomenorrhea and may be a risk factor;whereas TA15 allele may be a protective factor for patients.

Some outstanding progresses of relevant research are summarized,whilst the tasks and features of TCM informatics are discussed. The applications of random forest,self-organizing map,molecular docking and phylogenetic tree built with DNA polymorphism data etc. in TCM informatics are introduced. Some typical issues in the construction and maintenance of TCM database,involving ethnomedicine,phytochemistry and plant taxonomy etc.,are also discussed with abundant instances. TCM informatics integrating modern IT techniques motivates the development of TCM. The expansion of sources of TCM informatics and its internationalization is the fastrack of development.

Objective: To investigate the expression ofVEGF-Aand the correlation with the sentinel lymph nodes lymphangiogenesis and its metastasis in non-small cell lung cance(rNSCLC).Methods: The expression of VEGF-A was detected in 65 patients with NSCLC by SP immunohistochemistay. The expression of LYVE-1 was detected by immunofluorescence. The new lymphatic vessel density was counted by LYVE-1 and analyzed with clinicopathologic parameters. Results: The positive rate of VEGF-A was 72.3%(47/65),which was positively correlated with sentinel lympha nodes metastasis(r=0.453,P

0.05).Conclusion:QDs show no effect on cytobiological behavior of living MG-63 cells.

Objective:Assessment ofthe value of B-type natriuretic peptide levels combines TIMI risk scores in predictinglate mortality in patients with ST-segment Elevation Myocardial Infarction(STEMI).Methods:345 patients with STEMI were studied.Blood was drawn and the plasma concentration of BNP was determined within 24 hours after the onset of chest pain and the patients'TIMI risk score was measured.The all-cause mortality,including cardiac and non-cardiac deaths during Long-term follow-up,was recorded.Results:Mortality was 20.0%(n=69)during a follow-up of 314?208 days.The late mortality was related to higher TIMI risk scores(P;P

Fructose-1,6-bisphosphatase(FBPase) deficiency is a rare inherited metabolic disease,which is an autosomal recessive metabolic disorder.Affected patients usually present with metabolic crisis including severe hypoglycemia and metabolic acidosis.Each attack occurred with a similar sequence.The triggering factors are removed and then clinical status is improved dramatically.As patients are usually symptomless in the plateau stage,it is often misdiagnosed.Metabolite assay in blood and urine is very useful for the diagnosis of FBPase deficiency.FBPase is a key enzyme in gluconeogenesis.Deficiency of FBPase impairs the formation of glucose from all precursors.FBP1 gene mutation contributes to the disease.More than 30 mutation types have been reported.There is no specific treatment.Early diagnosis and appropriate life-style can prevent repetitive metabolic derangements,improving life quality of these children and ensuring successful pregnancy.

This article reviewed the feature and the advances in the application of micellar electrokinetic capillary chromatography(MECC),an important mean of high performance capillary electrophoresis(HPCE),for the analysis of complicated component of medicine,.The analysis of multi-component of routine medicine,chiral drugs,illicit drugs,Chinese medicine,drug and its metabolites were introduced.

Objective:To observe the inhibitory effects of survivin-specific siRNA on human lung cancer cell lines with different P53 statuses,and to analyse the relationship between P53 and survivin in vitro.Methods:A549(wild-type P53)and H1299(P53 absent)cells were transfected with chemosynthetic survivin siRNA.The effect of siRNA on cell proliferation was analysed by MTT assay.The changes of cell cycle and apoptosis were examined by flow cytometry.The expression of survivin mRNA was detected by RT-PCR.The protein expression of survivin,P53,P21 and PARP was examined by Western blotting.Results:Endogenous survivin level in A549 cells was lower than that in H1299 cells.The mRNA and protein expression of survivin was significantly lower in siRNA group than in blank control group and non-silencing siRNA group(P