Alpha-fetoprotein (AFP) was first discovered over 50 years ago, and is currently applied clinically in diagnosis, effects evaluation and relapse judgement of tumors especially for the primary hepatocellular carcinomas.And as an effective tumor marker, AFP test has become one of the essential items in physical examination.However, its clinical application is not limited to primary hepatocellular carcinomas,and its great application value has been proved in many benign diseases and other tumors (non-hepatic carcinomas).

Bronchial asthma is a chronic inflammatory disorder of the airways,caused by multiple genetic and environmental factors.Thtee key methods including candidate gene study,the positional cloning and genomewide association studies.are used for asthma susceptibility gene identification.There are many candidate genes that haye been identified as asthma susceptibility gene, including ADRB2,IL4R,FLG,NPPA,INPP4A,ADAM33, DPP10,OPN3,0RMDL3,PDE4D,CHBLI etc.Replication of genetic associations and demonstration of a functional mechanism for the associated gene variants are needed to COnfirm an asthmasusceptibility gene, this will help us to understand the aetiology of asthma and explore new drugs to treat asthma.

The intestinal mucosa is the biggest lymph organ in human body, which consists of the first line of defensive system with the respiratory mucosa and urinary mucosa. The intestinal mucosa is responsible to defend bacteria, viruses, dietary pathogens, non-steroid anti-inflammatory drug and so on. If the intestinal mucosal immune system is destroyed, the human will suffer many diseases such as infectious diseases,autoimmune diseases. Therefore, it plays an important part in physical health. T helper type 17 (Th17) cells different from Th1 cells and Th2 cells are a distinct lineage of T cells. The discovery of Th17 cells has ruined the constitutional pattern of Th1/Th2. Th17 cells, which are important parts of intestinal mucosal immune system,play critical roles in intestinal autoimmune diseases, tissue inflammation and the infection of various pathogenic bacteria. This paper will review the differentiation of Th17 cell and its function in intestinal mucosal barrier,intestinal autoimmune diseases and intestinal infectious diseases.

Systemic lupus erythematosus is an autoimmune-mediated inflammatory diseases in which immunologic derangement throughout this process.There are a varriety of abnormal immunoregulation,including the disturbance of Help T cell 1,Help T cell 2,Th17 cell and regulatory T cell( Treg cell),disfunction of the B cell subsets and deficiency of the immune tolerance,lead to the disturbance of related cytokines.Recently,it was indicated that the abnormal of cross regulation existencd between Help T cell 1 and Th17 cell,and the disfunction of non-antibody-dependent B cells may play an important role in the pathogenesis of SLE,According to different immune status,appropriate and targeted treatment was given in order to improve the prognosis of the patients with systemic lupus erythematosus.

Objective To understand ureaplasma urealyticum(Uu)and mycoplasma hominis(Mh)infection and drug resistance of mycoplasma from infertility patients in Lanzhou,to direct the clinical use of antibacterial drugs.Methods Uu and Mh from 1 278 cases were cultered and tested as well as their drug resistance were analyzed.Results 42.9%infertility patients showed Uu positive,7.0% showed Mh positive,and 9.0% infertility patients were mixed infection of both Uu and Mh.The sensitive antibacterial drugs were josamycin,doxycycline,minocycline hydrochloride,and clarithromycin,which the sensitive rates of both Uu and Mh were>80%,however,the sensitive rates of Uu and Mh against ciprofloxacin were 11.3% and 16.0%,respectively.Conclusion The mycoplasma infection rate was high in infertility patients,and the problem of drug resistance was serious.The results of drug sensitivity analysis should be recognized as a guide for rational drug use.

OBJECTIVE:To explore the characteristics and regularity of ADR induced by gynecological Chinese patent medi-cine,and to provide reference for rational use of gynecological Chinese patent medicine. METHODS:ADR reports were collected from gynecological ADR reporting information system of our hospital during 2008-2105;all ADR cases of Chinese patent medicine were collected and analyzed. RESULTS:No severe ADR was found among 41 cases of Chinese patent medicine-induced ADR,24 cases of new and common ADR(58.54%),17 cases of common ADR(41.46%);the incidence of ADR was in high level 1-3 d after medicating,being 97.6%. Main routes of administration were oral administration(19 cases,46.3%)and vaginal administra-tion(15 cases,36.6%)in ADR cases. Clinical manifestations were gastrointestinal reaction(29.97%),systemic reaction(19.15%) and mild injury of reproductive system(17.02%),etc. CONCLUSIONS:Chinese patent medicine is relatively safety in gynecologi-cal department. However,caution should be exercised in special cases and different medication.

Nephroblastoma is the most common pediatric renal tumor.The research progress of gene mutations in Wilms tumor children is reviewed in this paper.The WT1 gene,CTNNB1 gene and WTX gene plays a role in onset of Wilms tumor,and the MYCN gene,P53 gene and SIX1 gene may be associated with the prognosis.

Objective: To explore correlation between serum uric acid (UA) level and heart failure severity in patients with chronic pulmonary heart disease (CPHD).Methods:According to New York Heart Association (NYHA) cardiac function classification, a total of 98 CPHD patients were divided into class I~II group (n=36), class III group (n=31) and class IV group (n=31).Another 30 healthy subjects were enrolled as healthy control group simultaneously.Serum UA level was measured in all groups.Real time three-dimensional color Doppler echocardiography was used to measure left ventricular ejection fraction (LVEF) and left ventricular end-diastolic dimension (LVEDd), and the results were compared among all groups.Results: Compared with healthy control group, there were significant rise in serum UA level[(342.4±101.7) μmol/L vs.(459.6±110.3) μmol/L]and incidence rate of hyperuricemia (33.33% vs.58.16%) in CPHD group, P<0.05 both;along with NYHA class increased, there were significant rise in serum UA level[(350.7±103.4) μmol/L vs.(422.3±106.7) μmol/L vs.(491.8±113.2) μmol/L], LVEDd [(59.2±10.1)mm vs.(64.3±9.8)mm vs.(68.9±10.6)mm], and significant reduction in LVEF[(37.7±9.8)% vs.(31.9±8.6)% vs.(28.4±8.1)%]in CPHD patients, P<0.05 all;linear correlation analysis indicated that serum UA level was significant positively correlated with LVEDd (r=0.67, P=0.026), and significant inversely correlated with LVEF (r=-0.86, P=0.033).Conclusion: Serum uric acid level can be used as a reliable index evaluating heart failure severity in patients with chronic pulmonary heart disease.

0.05; emphysema in animals inhaling diphosgene into lower rispiratory tract (LRT) alone was obvious with Pden (0.59?0.05) being different from that of control animals, P

Objective To observe the clinical effect of poractant alfa injection(curosurf)combined with nasal continuous positive airway pressure(CPAP)in the treatment of neonatal respiratory distress syndrome(NRDS). Methods Fifty patients of infants with NRDS were divided into 25 cases of observation group(curosurf combined with CPAP)and 25 cases of control group(CPAP)according to the methods of the treatment.The differences in treatment efficacy rate and arterial blood gas index were compared.Results The efficacy rate of the observation group (88%)was higher than that of the control group(56%),the difference between the two groups was statistically significant(χ2 =4.861,P <0.05 ).After the treatment,the arterial blood gas index of the observation group were improved obviously compared with that before the treatment,the differences were statistically significant(all P <0.05).After the treatment,the arterial blood gas index of the control group were improved obviously compared with that before the treatment,the differences were statistically significant(all P <0.05).After treatment,the differences in pH value,PaO2 and PaCO2 of the observation group were statistically significant compared with the control group(t =6.00,8.49,4.46,all P <0.05).Conclusion Poractant alfa injection combined with nasal CPAP in the treatment of NRDS is better than using CPAP alone.