Objective To investigate serum angiopoietin-2(Ang-2)levels in non-small cell lung cancer(NSCLC)and its clinical significance.Methods Patients with NSCLC(n=98)and healthycontrols (n=30)were recruited into this study.Serum Ang-2 levels were measured using enzyme-linked immunosorbent assays(ELISA).The results were compared between patients with ifferent tumor stages,metastasis status,and postoperative recurrence 1 year after surgery.Results Patients with NSCLC had higher Ang-2 levels[median 3 230.5 ng/L,range(1 231.6～4 612.5)ng/L ]than the control group [median 831.7 ng/L,range(592.4-1 701.1)ng/L,U=15,P<0.01].Serum Ang-2 levels in stage Ⅲpatients[median 2 311.7 ng/Lrange(1 512.9～3 530.7)ng/L]were significantly higher than stage I [median 1 593.6 ng/L,range(1 235.2～2 763.1)ng/L,H=27.66,P<0.01]and stage Ⅱ patients [median 1 581.4 ng/L,range(1 231.6～2 852.3)ng/L,H=27.66,P<0.01],but significantly lower than stage Ⅳ patients[median 3 808.2 ng/L,range(2 235.8～4 612.5)ng/L,H=65.6P<0.01].Serum Ang-2 levels were higher in patients with distant metastasis or postoperative recurrence than those without them.Conclusion Serum Ang-2 level in patients with NSCLC could be a useful marker for metastasis and postoperative recurrence.

OBJECTIVE To evaluate the value of HPV-DNA test in the screening of cerical lesions.METHODS Patients with cervical lesions were detected by HPV-DNA test Papanicolaou(Pap) smear and pathological inspection,and the pathological results were regarded as gold standard.From the 178 positive cases,we compared the sensitivity of HPV-DNA test and Pap smear,and the HPV-DNA detection rate from patients with different histocytological classification.RESULTS All of 178 cases were positive in pathological inspection,the HPV-DNA test sensitivity was 85.4%,whereas Pap smear was 50.6%,the difference between HPV-DNA test and Pap smear was significant(P

0.05).While there were significant differences when comparing CINⅠwith CINⅡ-Ⅲ(?2=14.97,P

OBJECTIVE To detect and analyze the results and significance of HBV-DNA,HBeAg and PreS1 of HBV infective sera.METHODS Routine detection and PreS1 antigen of 450 sera were tested by ELISA,and HBV-DNA was detected by fluorescent quantitation PCR.RESULTS The positive rates of HBV-DNA,HBeAg and PreS1 were 74.4%,48.9% and 63.3%,respectively,in 450 cases of HBV infective sera.Among 285 PreS1-positive samples,the positive cases of HBeAg and HBV-DNA were 190 and 270,respectively.There were significant difference(P0.05)among PreS1,HBV-DNA and PreS1,and they three were in positive correlation.CONCLUSIONS PreS1 and HBV-DNA are more sensitive than HBeAg,and PreS1 is better coincided with HBV-DNA.They can reflect the infection and replication condition of HBV.Therefore,it has important clinical meaning for the diagnosis and therapy of HBV to simultaneously used HBV-DNA,HBeAg and PreS1.

OBJECTIVE To analyze the susceptible factors and resistance of Acinetobacter baumannii in ICU,in order to create a novel thought of effective prevention and control of A. baumannii cross-infection. METHODS Specimens were collected from ICU patients with infections from Sep 2006 to Sep 2008. The infection characteristic was analyzed and the drug sensitivity was tested to know the risk factors of infection retrospectively. RESULTS The factors of low immunity,severe wound,invasive operation and using ventilator led to the serious cross-infection of A. baumannii in ICU .In vitro drug susceptibility test,the sensitive rates only to cefoperazone/sulbactam and amikacin were higher. They were 65.09% and 72.64%,respectively. CONCLUSIONS It has great significance in the prevention and control of A. baumannii cross-infection in ICU to strengthen the environment disinfection,were education of nosocomial infection knowledge among medical staff,executeing the hand-washing system strictly,useing the antibiotics rationally,keeping the susceptible crowd and adopting de-escalation strategy of prevention and control.

OBJECTIVE To survey the distribution of the pathogenic microorganisms in biles collected from cholelithiasis patients and their susceptibility to antibiotics,to guide the selection of reasonable antibiotics.METHODS Totally 546 bile samples were cultured and tested for antibiotics susceptibility.RESULTS Bacteria were cultured from 332 samples with a positive rate of 60.81%,the first three were Escherichia coli(32.96%),Enterococcus faecalis(12.74%),and Klebsiella pneumoniae(9.97%).The pathogenic microorganisms were more sensitive to the third and fourth generation cephalosporins,antibiotic/?lactamase inhibitor combination,carbopenems and aminoglycosides antibiotics,while less sensitive to the first and second generation cephalosporins,penicillins,macrolide antibiotics and fluoroquinolones.CONCLUSIONS The pathogenic microorganisms in bile are very various,E.coli,E.faecalis and K.pneumoniae are usually cultured,fungous and anaerobic infections cannot be ignored.Doctors should pay more attention to analyze the bacterial resistance profile and use the antibiotics properly.

OBJECTIVE To investigate the change in antibiotic resistance of Pseudomonas aeruginosa(PAE) in our hospital,for the guidance of rational use of antibacterials. METHODS We reviewed the isolation rates of antimicrobial agents from 2004 to 2007,and its construction ratio among the commonly encountered Gram-negative bacilli,and the resistance rates of P.aeruginosa to 14 kinds. RESULTS We totally isolated 823 strains of PAE and the isolation rates of PAE was 13.3% in 2004 but 18.5% in 2007.In the four years,there was the highest isolation rate of 72.54% in sputum and throat mucus specimens.The lowest resistance rate was to amikacin(from 12.2% to 6.21%,the average was 8.75%),and the next was to cefoperazone/sulbactam(from 6.10% to 12.26%,the average was 8.99%);the total resistance rates to ticarcillin and piperacillin was more than 36.00% and there was a lower resistance rate to ticarcillin/clavulanic acid(32.93%)after adding the enzyme inhibitor,while to piperacillin/tazobactam was 20.16%.Drug-resistance rate to ceftazidime was from 19.51% to 43.17%(the average was 33.05%);to cefepime from 22.34% to 34.16%(the average was 29.40%);to imipenem from 19.51% to 23.29%(the average was 22.60%);to meropenem from 11.17% to 18.18%(the average was 15.43%);to the fluoroquinolones antibiotics ciprofloxacin from 32.61% to 44.59%(the average was 38.52%);and to levofloxacin was 26.40% in average. CONCLUSIONS There is commonly increasing resistance of PAE.Reviewing the resistance situation dynamically and selecting the antibiotics reasonably can produce great significance for clinical therapy and postpon its multidrug-resistance.

Objective To explore the changes of the level of serum HCY in patients with unstable angina pectoris(UAP). Methods A total of 44 UAP patients were selected in our hospital, 53 patients with AMI and 50 healthy people were selected as control group. 2 mL vein blood was collected and the serum level of HCY was measured. Diagnostic accuracies of HCY was evaluated by receiver operating characteristic (ROC)curve analysis. Results The level of HCY [(16.33±2.06)μmol/L] in UAP group was higher than HCY [(10.43±2.32)μmol/L] in the healthy control group, lower than AMI group [(18.52±3.29)μmol/L], and the differences were statistically significant(P<0.05).Using a cut-off value of 12.26 μmol/L,HCY had the sensitivity of 72.1%,specificity of 76.0%. Conclusion The sensitivity and specificity of HCY on the early diagnosis of UAP was well, which could be a useful predictor for UAP and is worthy of clinical appli-cation.