Objective:To reconstruct the curriculum based on the systematization of working process, and discuss the teaching effect of task-driven teaching method in microbiology examination teaching reform under the "student-centered and teacher-guided" mixed teaching mode.Methods:A total of 120 medical laboratory students were selected as research objects, and they were divided into experimental group and control group. The experimental group implemented the reconstructed curriculum content using task-driven teaching method under the mixed teaching mode, and the control group used traditional teaching method. At the end of the course, the questionnaire survey and final examination were combined to evaluate the teaching effect. SPSS 21.0 was used for statistical analysis to evaluate the differences of the teaching methods.Results:The questionnaire survey showed a generally higher evaluation on the teaching effect of the students after the reform, and the performance of the experimental group were significantly higher than that of the control group, with significant differences ( P<0.005). Conclusion:The task-driven teaching under the mixed teaching mode to carry out the teaching form combining theory with practice aimed at strengthening skills training can greatly promote the improvement of students' professional knowledge and skills as well as the cultivation of their professional qualities, so that the course of microbiological examination technology can better meet the requirements of higher vocational medical examination training objectives.