1.Nephroblastomatosis and nephroblastoma: report of a case.
Xiao-li HU ; Lan-yun SONG ; Lin-sheng ZHAO ; Pei-ru NING ; Li ZHAO
Chinese Journal of Pathology 2013;42(12):841-842
Humans
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Infant
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Kidney Neoplasms
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metabolism
;
pathology
;
surgery
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Male
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Mucin-1
;
metabolism
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Nephrectomy
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Precancerous Conditions
;
metabolism
;
pathology
;
surgery
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Vimentin
;
metabolism
;
WT1 Proteins
;
metabolism
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Wilms Tumor
;
metabolism
;
pathology
;
surgery
2.A study of integrin beta3 mRNA expression and its correlation with microvascular density, progression and prognosis of gastric carcinomas.
Zhong-sheng ZHAO ; Guo-qing RU ; Jie MA
Chinese Journal of Pathology 2004;33(1):16-20
OBJECTIVETo investigate mRNA expression of integrin beta3 in gastric carcinoma, its correlation with microvascular density (MVD), growth-pattern, invasion, metastasis and prognosis.
METHODSIn situ hybridization and immunohistochemistry techniques were used to study the expression of integrin beta3 mRNA and CD34 protein in 105 gastric carcinoma specimens.
RESULTSIn situ hybridization revealed a 30% (6/20) positive rate of integrin beta3 mRNA in non-tumor gastric mucosa, which was significantly lower than that of the gastric cancer group (61.0%, 64/105, chi(2) = 8.85, P = 0.037); In infiltrating type cases (70.2%, 40/57), stage III - IV (72.1%, 44/61), lymphatic metastasis (68.6%, 48/70) and distant metastasis (85.7%, 36/42), the positive expression rates were significantly higher than those of the expanding type (chi(2) = 14.97, P = 0.002), stage I - II (chi(2) = 15.21, P = 0.015), non-lymphatic metastasis (chi(2) = 17.89, P = 0.025) and non-distant metastasis (chi(2) = 20.22, P = 0.005; chi(2) = 21.35, P = 0.035); its mean MVD was also significantly higher than that of the expanding type (t = 10.105, P = 0.001), stage I approximately II (t = 5.961, P = 0.001), non-lymphatic metastasis (t = 3.819, P = 0.01)and non-distant metastasis (t = 10.578, P = 0.001; t = 7.882, P = 0.001), respectively; The mean MVD (41.02 +/- 8.55)/0.72 mm(2) of the integrin beta3 mRNA positive expression group was significantly higher than (25.26 +/- 11.25)/0.72 mm(2) of the negative expression group. There was a positive relation between MVD and integrin beta3 mRNA expression in the tumor (r(s) = 0.316, P = 0.001). The mean survival time in cases with positive integrin beta3 mRNA expression or MVD value >or= 39.5 was significantly shorter than that in cases with negative expression or MVD value < 39.5.
CONCLUSIONSIntegrin beta3 expression correlates with enhanced tumor angiogenesis and may play a role in the invasion and nodal metastasis of gastric carcinoma, therefore it may serve as a prognostic marker of gastric cancer.
Adult ; Aged ; Disease Progression ; Female ; Humans ; Integrin beta3 ; genetics ; Male ; Middle Aged ; Neovascularization, Pathologic ; etiology ; Prognosis ; RNA, Messenger ; analysis ; Stomach Neoplasms ; blood supply ; metabolism ; pathology ; Survival Rate
3.Carcinoid tumor of common bile duct: report of a case in pediatric patient.
Lan-yun SONG ; Xiao-li HU ; Lin-sheng ZHAO ; Pei-ru NING
Chinese Journal of Pathology 2011;40(1):54-55
Adenocarcinoma
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metabolism
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pathology
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Carcinoid Tumor
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metabolism
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pathology
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surgery
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Child
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Chromogranin A
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metabolism
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Common Bile Duct
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pathology
;
surgery
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Common Bile Duct Neoplasms
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metabolism
;
pathology
;
surgery
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Diagnosis, Differential
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Duodenum
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pathology
;
surgery
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Gallbladder
;
pathology
;
surgery
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Humans
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Keratins
;
metabolism
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Lymphoma
;
metabolism
;
pathology
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Male
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Mucin-1
;
metabolism
;
Neoplasm Invasiveness
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Rhabdomyosarcoma
;
metabolism
;
pathology
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Stomach
;
pathology
;
surgery
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Synaptophysin
;
metabolism
4.Epigastric heteropagus twins: report of a case.
Xiao-li HU ; Lan-yun SONG ; Lin-sheng ZHAO ; Pei-ru NING
Chinese Journal of Pathology 2009;38(7):491-492
Abnormalities, Multiple
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pathology
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surgery
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Humans
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Infant, Newborn
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Male
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Twins, Conjoined
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pathology
;
surgery
5.Correlative studies on uPAR receptor mRNA expressions with vascular endothelial growth factor, microvessel density, progression and survival time of gastric carcinomas.
Zhong-sheng ZHAO ; Guo-qing RU ; Jie MA ; Wen-juan XU ; Zhong MENG
Chinese Journal of Pathology 2005;34(5):306-307
Adenocarcinoma
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blood supply
;
metabolism
;
mortality
;
secondary
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Adult
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Aged
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Female
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Humans
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Lymphatic Metastasis
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Male
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Microcirculation
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Middle Aged
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Neovascularization, Pathologic
;
metabolism
;
pathology
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Prognosis
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RNA, Messenger
;
biosynthesis
;
genetics
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Receptors, Cell Surface
;
biosynthesis
;
genetics
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Receptors, Urokinase Plasminogen Activator
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Stomach Neoplasms
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blood supply
;
metabolism
;
mortality
;
pathology
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Survival Rate
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Vascular Endothelial Growth Factor A
;
metabolism
7.Significance of COX2 and beta-catenin expression in nephroblastoma.
Xiao-Li HU ; Lin-Sheng ZHAO ; Lan-Yun SONG ; Pei-Ru NING
Chinese Journal of Pathology 2008;37(10):698-699
Child
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Child, Preschool
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Female
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Gene Expression Regulation, Neoplastic
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Humans
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Infant
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Male
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Wilms Tumor
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genetics
;
metabolism
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Wnt Proteins
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genetics
;
metabolism
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beta Catenin
;
genetics
;
metabolism
8.mRNA expression of syndecan-1 and heparanase in relation to tumor progression and prognosis of gastric carcinoma.
Guo-qing RU ; Zhong-sheng ZHAO ; Qi-le TANG ; Wen-juan XU
Chinese Journal of Surgery 2006;44(15):1062-1064
OBJECTIVETo investigate mRNA expression of syndecan-1 and heparanase in gastric carcinoma, and their correlation with the growth-pattern, invasion, metastasis and prognosis of gastric carcinoma.
METHODSIn situ hybridization technique was used to examine mRNA expression of syndecan-1 and heparanase in 118 specimens of gastric carcinoma.
RESULTSThe positive rates of syndecan-1 mRNA and heparanase mRNA were 42.4% and 55.9%, respectively. The expression of syndecan-1 mRNA and heparanase mRNA were related to tumor invasion depth (chi(2) = 32.95, P = 0.001; chi(2) = 23.19, P = 0.001), vessel invasion (chi(2) = 46.22, P = 0.001; chi(2) = 33.78, P = 0.001), lymph node (chi(2) = 28.62, P = 0.001; chi(2) = 25.43, P = 0.001) and distant metastasis (chi(2) = 63.30, P = 0.001; chi(2) = 65.76, P = 0.001), and syndecan-1 mRNA positive expression was related to tumor size (chi(2) = 6.25, P = 0.012). There was a negative relationship between Syndecan-1 mRNA and heparanase mRNA expression (r = -0.844, P = 0.001). The mean survival time of cases with low expression of syndecan-1 mRNA was significantly shorter than that of cases with high expression (r = 36.48, P = 0.001), and meanwhile, the mean survival time of heparanase mRNA positive cases was significantly shorter than that of cases with negative expression (r = 34.41, P = 0.001).
CONCLUSIONSThe mRNA expression of syndecan-1 and heparanase can predict the invasion and metastasis of gastric carcinoma, and can be used as markers of prognosis of gastric carcinoma.
Adult ; Aged ; Female ; Follow-Up Studies ; Glucuronidase ; genetics ; metabolism ; Humans ; In Situ Hybridization ; Lymphatic Metastasis ; Male ; Middle Aged ; RNA, Messenger ; genetics ; Stomach Neoplasms ; metabolism ; mortality ; pathology ; Survival Rate ; Syndecans ; genetics ; metabolism
10.Expressions of hypoxia inducible factor-1alpha and insulin-like growth factor-II in gastric carcinoma: correlation with angiogenesis and prognosis.
Guo-Qing RU ; Zhong-Sheng ZHAO ; Qi-le TANG ; Wen-Juan XU
Chinese Journal of Surgery 2007;45(13):905-908
OBJECTIVETo investigate relationship between expressions of hypoxia inducible factor-1alpha (HIF-1alpha) and insulin-like growth factor-II (IGF-II) as well as their correlation with angiogenesis and prognosis in gastric carcinoma.
METHODSHIF-1alpha and IGF-II mRNA expression were analyzed using in situ hybridization, microvessel density (MVD) was determined by anti-CD34 immunostaining in 118 cases gastric carcinoma.
RESULTSThe positive rates of HIF-1alpha mRNA and IGF-II mRNA were 49.2% and 47.4%, respectively. In stage T3-T4 cases, positive rates of HIF-1alpha and IGF-II mRNA expression, the frequencies of vessel invasion, lymph node and distant metastasis were significantly higher than those in stage T1-T2 cases. The mean MVD in stage T3-T4 tumors, vessel invasion, lymph node metastasis and distant metastasis were significantly more frequent than those in stage T1-T2 tumors. The mean MVD in tumors with positive HIF-1alpha and IGF-II mRNA expression was significantly higher than those in tumors without HIF-1alpha and IGF-II mRNA expression. The expression of HIF-1alpha was positively correlated with IGF-II mRNA. There were positive correlations between MVD and expression of HIF-1alpha and IGF-II mRNA, respectively. The mean survival time and 5-year survival rate in cases with positive HIF-1alpha and VEGF expression and MVD value >/= 41.5 were significantly shorter than those in cases with negative HIF-1alpha and VEGF expression and MVD value < 41.5.
CONCLUSIONSHIF-1alpha and IGF-II play an important role in tumor invasion and metastasis, especially in tumor angiogenesis. So test of the expression of HIF-1alpha and IGF-II may act as a useful index of treatment and prognosis.
Adult ; Aged ; Antigens, CD34 ; analysis ; Female ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit ; genetics ; metabolism ; Immunohistochemistry ; In Situ Hybridization ; Insulin-Like Growth Factor II ; metabolism ; Male ; Middle Aged ; Neovascularization, Pathologic ; genetics ; metabolism ; pathology ; Prognosis ; RNA, Messenger ; genetics ; metabolism ; Stomach Neoplasms ; blood supply ; genetics ; metabolism ; Survival Analysis