Body Fluids ; cytology ; Cytodiagnosis ; methods ; Humans ; Immunohistochemistry ; methods ; Lung Neoplasms ; pathology ; Pleural Effusion, Malignant ; pathology ; Quality Control ; Specimen Handling ; standards

Comparative analysis of characteristic of species and esterase-isoenzyme of isolates of Polyporus umbel-latus from different regions were processed. The results indicated that isolates of Jizhaoling ( Z) and Zhushiling (ZJ) have significant differences in characteristic, and enzymatic band types of the two species also have significant differences. The homology at genetics between the two isolates is 0% , and consanguinity between the two i-solates is the farthest.

Viral hepatitis was the most common infectious disease in china. But the diagnosis and treatment were varied because the viral hepatitis patients were hospitalized in different kinds of hospital such as infectious disease hospital, general hospital and Chinese medical hospital. It was necessary to know clinical characters and information of viral hepatitis patients in different hospitals. The general information, subtype distribution, prognosis, complication, medication and relations of onset with solar term from 41 180 viral hepatitis patients based on HIS data were analyzed. It was found that the age of patients between 18 to 59 years old was most; most patients were males. The national basic medical insurance was the most type of payment. The outcome of viral hepatitis in the youth and female were better than that in the old and male. Acute hepatitis was easer to restore than chronic hepatitis. Liver cirrhosis and hepatocellular carcinoma were the two most complications. The peak of onset was during summer solstice, slight heat and great heat. The most common Chinese medicine was Diammonium glycyrrhizinate and the most common western medicine was reduced glutathione. The combination of D. glycyrrhizinate with reduced glutathione, polyene phosphatidylcholine and thymosin was the main pattern. But It was not knew if the combination of western and Chinese medicine was the most effective therapy to protect liver function. It was necessary to take deeply research of the relationship between the combination therapy and their effectiveness.
Adolescent ; Adult ; Aged ; Antiviral Agents ; therapeutic use ; Female ; Glutathione ; therapeutic use ; Glycyrrhizic Acid ; therapeutic use ; Hepatitis, Viral, Human ; drug therapy ; Hospitals ; Humans ; Male ; Middle Aged ; Young Adult

OBJECTIVETo observe the development of arrhythmia induced by ischemia/reperfusion (I/R) of the right coronary artery in rabbits and the intervening effect of Chinese medicine Qiangxin Fumai Granule (QFG), a Chinese preparation for activating yang and promoting blood circulation, on it.

METHODSRabbit right coronary artery I/R model was adopted. Forty healthy adult rabbits were randomly divided into 5 groups, the sham-operation group, the model group, the atropine group, the high-dose QFG group, and the low-dose QFG group, eight in each group. The drugs were administered via duodenal perfusion 10 min after ischemia. The changes of AA interval before and after medication were observed and the scores of arrhythmia were calculated.

RESULTSDuring ischemia period, AA interval prolonged for more than 40 ms in the model group, and the scores of arrhythmia showed a rising trend along with the prolonging of ischemia, with the presence of atrial-ventricular block (AVB) and aggravating of sinus and atrial arrhythmia; during reperfusion period, the incidence of AVB decreased, and AA interval somewhat decreased. The AA intervals and scores of arrhythmia in the high and low-dose QFG groups were significantly lower than those in the model group respectively (P < 0.01 or P < 0.05).

CONCLUSIONQFG is beneficial for shortening AA interval and preventing arrhythmia induced by I/R.

Animals ; Anti-Arrhythmia Agents ; administration & dosage ; pharmacology ; therapeutic use ; Arrhythmias, Cardiac ; drug therapy ; physiopathology ; Coronary Artery Disease ; complications ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal ; administration & dosage ; pharmacology ; therapeutic use ; Female ; Male ; Myocardial Ischemia ; complications ; Myocardial Reperfusion Injury ; etiology ; physiopathology ; Phytotherapy ; Rabbits ; Random Allocation ; Treatment Outcome

Objective To explore the neuroprotective effects of orientin in rats after cerebral ischemia-reperfusion and its possible mechanisms.Methods SD male rats were randomly divided into five groups,with 15 rats in each group:sham group,model group,control group,experimental Ⅰ group,experimental Ⅱ group.Middle cerebral artery occlusion (MCAO) model was established by suture method in each group except sham group.For sham group,separate the carotid artery only.The 2.9 mg · mL-1 of orientin was administrated at 0 h and 12 h of reperfusion in control group and two experimental groups.At 0.5,24 h before the operation,autophagy inducer (rapamycin 2.24 mg · mL-1) and autophagy inhibitor [3-methyladenine (3-MA) 3.0 mg · mL-1] were administrated in experimental Ⅰ group and experimental Ⅱ group,respectively.The same dosage of normal saline was administrated to other groups.The neurological deficit scores were evaluated and brain infarct volume was determined by 2,3,5-triphenyltetrazolium chloride (TTC) staining after 24 h of reperfusion.Moreover,protein immunoblotting was used to observe the protein levels of Beclin1 and microtubule-associated protein1 light chain3 (LC3).Results The brain infarct volume in model group was (36.63 ±2.06)％,which was higher than sham group (0.67 ±0.12)％,the difference was statistically significant (P ＜0.01).The brain infarct volume in control group,experimental Ⅰ and Ⅱ group were (14.71 ± 1.63)％,(25.22 ± 1.58) ％ and (6.45 ± 1.07) ％,respectively;compared with model group and three drugs groups,the difference were statistically significant (all P ＜ 0.05).The expression of autophagy protein Beclin1 (APB) in model group,sham group were 3.16 ±0.17,(1.00 ±0.06) while control group,experimental Ⅰ and Ⅱ groups were,1.67 ±0.15,2.24 ±0.13 and 1.21 ±0.09,respectively.For the protein expression of LC3 in model group,sham group,control group,experimental Ⅰ and Ⅱ groups were 2.98 ± 0.12,1.00 ± 0.05,1.54 ± 0.13,2.24 ± 0.12,1.49 ± 0.17.Compared with sham group and model group,the difference were statistically significant (all P ＜0.01).Compared with model group and three drugs groups,the difference were statistically significant (all P ＜ 0.05).Compared with control group and experimental Ⅰ group,the difference of the protein expression were statistically significant (all P ＜ 0.05).Conclusion The neuroprotective effects of orientin in rats after cerebral ischemia-reperfusion may be mediated through inhibition of autophagy.

Apoptosis ; physiology ; Carcinoma, Hepatocellular ; enzymology ; pathology ; Carrier Proteins ; biosynthesis ; genetics ; DNA-Binding Proteins ; metabolism ; Gene Targeting ; Humans ; Liver Neoplasms ; enzymology ; pathology ; RNA Interference ; Telomerase ; metabolism ; Tumor Cells, Cultured

Adolescent ; Adolescent Development ; Child ; Child Development ; China ; epidemiology ; Female ; Humans ; Male ; Obesity ; epidemiology ; Prevalence

AIMTo evaluate in vitro release of lycopene microcapsules. Pharmacokinetic parameters of lycopene microcapsule and lycopene powder as reference were estimated after a single dose of oral administration to dogs. The relationship between in vitro dissolution and in vivo absorption was investigated.

METHODSThe content of lycopene in the release medium was determined by UV spectroscopy method. Health hybrid male dogs were used as experiment subjects and lycopene powder used as standard to estimate the pharmacokinetics of lycopene microcapsules. HPLC method was used to assay the concentration of lycopene in dog plasma. Pharmacokinetics parameters were estimated by 3P87 program. The drug release percentage in stimulated intestinal fluid was compared with the absorption at a given time point.

RESULTSThe release profiles of lycopene from microcapsule showed that the lycopene gelatin microcapsule exhibited enteric property. The pharmacokinetics parameters estimated after oral administration of lycopene powder and lycopene microcapsule in a single dose of 2.5 mg x kg(-1) body weight to dogs were 7.30 h, 15.06 h for T1/2alpha; 28.10 h, 46.76 h for T1/2beta; 22.32 h, 41.03 h for T(max); 1.67 microg x h x L(-1), 2.08 microg x h x L(-1) for AUC(0-infinity), respectively. The concentration-time curves could be fitted to a two-compartment model for both the lycopene powder and the lycopene microcapsule analyzed by 3P87 program. The relationship between in vitro dissolution and in vivo absorption was found to have good correlation (r = 0. 981 9) was found.

CONCLUSIONIt could be concluded that lycopene microcapsule was a sustained release dosage form. The result of release in vitro could be used to predict the absorption in vivo.

Administration, Oral ; Animals ; Antioxidants ; administration & dosage ; pharmacokinetics ; Area Under Curve ; Biological Availability ; Capsules ; Carotenoids ; administration & dosage ; pharmacokinetics ; Delayed-Action Preparations ; Dogs ; Male

OBJECTIVETo summarize the clinical experience from treatment of patients with severe acute respiratory syndrome (SARS).

METHODSRetrospective analysis of seven patients with SARS in Ditan hospital treated since April 22 in 2004 was performed.

RESULTSIn the 7 patients, 2 were male, 5 were female, and the average age was (35.3 plus/minus 11.3) years. The main clinical manifestations were fever, cough, minor or serious dyspnea, nausea, signs of injury to other organs, and so on. The treatment regiments included oxygen, small dosage and short period of methylprednisolone (1 to 2 mg/kg), use of ventilator, psychological intervention, and treatment of underlying diseases, after which, all the 7 patients recovered.

CONCLUSIONRational use of methylprednisolone and timely use of ventilator were the key steps of treatment.

Adult ; Anti-Inflammatory Agents ; therapeutic use ; Combined Modality Therapy ; Cross Infection ; drug therapy ; therapy ; transmission ; Female ; Humans ; Infectious Disease Transmission, Patient-to-Professional ; Male ; Methylprednisolone ; therapeutic use ; Middle Aged ; Oxygen Inhalation Therapy ; Retrospective Studies ; Severe Acute Respiratory Syndrome ; therapy ; transmission ; Ventilators, Mechanical