Objective To evaluate the effective of emergent endoscopic injection sclerotherapy ( EIS) for lethal bleeding from esophagogastric varices. Methods In this study, endoscopic injection of 5% sodium morrhuate was used to control esophagogastric variceal bleeding in 176 patients. In all 209 cases, 181 cases suffered from acute lethal bleeding from esophagogastric varices received endoscopic variceal sclerotherapy under the modified Senstaken-Blakemore tube compression. Results The exact bleeding site at the first procedure could be visualized in 166(94. 3% ) patients. The effective rate of EIS was 96. 2% . Multiple procedures were required in the 21 cases of relapsed bleeding. Complications of EIS occurred in 15 cases (8. 5% ) , of which 3(1. 7% ) died. Conclusion Endoscopic injection sclerotherapy under the modified Senstaken-Blakemore tube compression is relatively safe and effective procedure in the treatment of lethal bleeding from esophagogastric varices.

To investigate the distribution of Hepatitis B virus (HBV) genotypes among the population of Dai nationality in Xishuangbanna, Yurman Province HBV genotypes of the Serum samples were tested by PCR-RFLP. This is the first time to discover the B+E genotypes in China. This finding provides new information for understanding the distribution of HBV genotype in China and a provides a basis for establishing a Chinese gene bank.

Occupational Medicine ; education

Objective To investigate the causes, clinical features, treatment and prognosis of gastrointestinal bleeding (GIB) patients in emergency department.Methods To analyze prospectively the clinical data of 168 GIB patients admitted to the emergency department of Peking Union Medical College Hospital during 2006.1-2006.12.Results (1) General data; male: female = 1.75:1 ( 107: 61) , mean age 13-87(56.5 ±17.8) years with a peak in 60-69 years.The percentage of old patients was significantly higher than that of young and middle age ( 52.4% vs 19.6% and 28.0% , P = 0.000 ).( 2 ) The incidence of acute gastric mucosal lesion in patients taking non-steroidal antiinflammatory drugs ( NSAIDs) ( 18.5% ) was significantly higher than that in patients not taking( 0.7% , P = 0.000 ).( 3 ) 86.9% ( 146/168 ) of the patients had anemia.(4) More patients who took emergency gastroscopy could be diagnosed than those patients who did not (89.4% vs 58.5% , P =0.000), while no significant difference could be seen between patients who took emergency enteroscopy and patients who had non-emergency gastroscopy (20.0% vs 57.9% , P =0.315).(5)The hemostatic ratio in GIB patients due to peptic ulcer was obviously higher than that in GIB patients due to other causes (86.0% vs 40.7% ,P =0.000).The rate of emergency operation for GIB patients was 1.8%.Conclusions Most of the GIB patients admitted to tertiary general hospitals are elderly males.NSAIDs administration is one of the most important causes of upper GIB.Upper GIB patients should have gastroscopy as soon as possible, while emergency coloscopy is of little significance in cases with lower gastrointestinal hemorrhage.

Objective To verify the effects of different conc entrations of auecifemine on the function of osteoblast cell expression and oste ogenic ability in vitro . Methods Iliac spongy bone specimens were obtained from twel ve adult patients scheduled surgery. After the bone pieces were treated with col lagenase-trypsin, osteoblasts were obtained from cancellous bones. Then the ost eoblasts were purified and cultivated. They were cultured in medium with various concentrations(1?10 -11 , 1?10 -9 , 1?10 -7 , 1?10 -6 , 1 ?10 -5 , 5?10 -5 mol?L -1 ). The alkaline phosphatase (ALP) acti vity, osterocalcin and osteogenic ability were examined by biochemical assay and radioimmunologic assay (RIA), respectively. Results The co rrelation of auecifemine of different concentrations with ALP activity and osteo calcin secretion was positive. Auecifemine of lower concentrations (1?10 -11 mol?L -1 , 1?10 -9 mol?L -1 and 1?10 -7 mol?L -1 ) s howed the stimulating effect on osteogenic ability and but not higher concentrat ion. Conclusion Estrogen can increase osteoblast ALP activi ty, osteocalcin production, and osteogenic ability.

Objective: To explore the effect of transgenic metallothionein (MT) mushroom on anti-radiation ability of mice. Method: Dried power of transgenic MT mushroom was mixed into the feed of ICR mice. After being fed for 23 d, they were irradiated by 5.0Gy 60Co-?rays. Then their anti-radiation ability was determined after being fed 7 d again. Results: Transgenic MT mushroom can evidently alleviate the injury of platelet and spleen induced by radiation, and apparently increase SOD activity and reduce MDA content in irradiated mice’s serum. Conclusion: Transgenic MT mushroom can improve anti-oxidation ability of irradiated mice.

To investigate the distribution of hepatitis B virus (HBV) genotypes and subgenotypes among the Bai nationality in Dali, a total of 100 serum samples from patients with chronic HBV-infection were collected for the detection of HBV genotypes and subgenotypes by genotype-specific primers and restriction fragment length polymorphism (RLFP), respectively. Among the 100 samples, the proportions of genotype B, C and mixed genotype (B+C) were 41%, 25% and 34%, respectively. All the genotype B strains belonged to subgenotype Ba. In genotype C, 84% were Subgenotype Cs and 12% were subgenotype Ce. The distribution of genotypes B, C and B+C showed no significant difference between male and female patients (P=0.182) and among the age groups of patients (P=0.812). The rates of HBeAg/HBeAg positivity were no significantly different among genotypes B, genotype C and mixed genotype (B+C) (P=0.077/P=0.663). In Dali, genotypes B, B+C and C existed among Bai nationality with chronic HBV-infection, and genotype B was the major genotype. Subgenotypes Ba and Cs were the predominant strains in patients with HBV genotype B/C infection. The most prominent characteristic was the higher prevalent rate of mixed genotype (B+C) in patients.

OBJECTIVETo study the biomechanical relationship between iliac rotation displacement and L(4,5) disc degeneration, and to provide clinical evidences for the prevention and treatment of L(4,5) disc degeneration and herniation.

METHODSFrom March 2012 to February 2014,68 patients with lumbar disc herniation combined with sacroiliac joint disorders were selected. Among them, 42 patients with L(4,5) disc herniation combined with sacroiliac joint disorders included 22 males and 20 females, ranging in age from 19 to 63 years old, with an average of (51.78 +/- 20.18) years old, and the duration of the disease ranged from 1 to 126 months with an average of (11.18 +/- 9.23) months. Twenty-six patients with L5S1 disc herniation combined with sacroiliac joint disorders included 11 males and 15 females, ranging in age from18 to 65 years old with an average of (45.53 +/- 27.23) years old, and the duration of the disease ranged from 0.5 to 103 months with an average of (11.99 +/- 12.56) months. Sixty-eight anteroposterior lumbar radiographs, 68 lateral lumbar radiographs,and 68 pelvic plain films were taken. The degree of lumbar scoliosis, pelvic tilt,and disc thickness were measured. The correlation between pelvic tilt and lumbar scoliosis ,lumbar scoliosis and disc thickness were studied by using linear and regression methods. The hiomechanical analysis was performed.

RESULTSThere was a positive correlation between pelvic tilt and lumbar scoliosis in patients with L(4,5) disk herniation (R=0.49, P=0.00). There was a causal relationship and good linear proportional relationship (Y=3.05+1.07X, P=0.00) in the two variables. There was a negative correlation between lumbar scoliosis and intervertebral space in male patients with L (4,5) disk herniation (R = -0.50, P=0.01). There was a causal relationship and good linear proportional relationship in the two variables (Y=13.09-0.27X, P=0.02). But there was a positive correlation between lumbar scoliosis and intervertebral space in male patients with L5S1 disk herniation (R=0.46, P=0.04).

CONCLUSIONIliac rotational displacement are closely related with L(4,5) disc degeneration and herniation in biomechanics. A new concepts and therapeutic approach is provided for clinical treatment of chronic and refractory herniation of L(4,5) disc in patients

Adult ; Biomechanical Phenomena ; Female ; Humans ; Intervertebral Disc Degeneration ; physiopathology ; Intervertebral Disc Displacement ; physiopathology ; Lumbar Vertebrae ; physiopathology ; Male ; Rotation ; Sacroiliac Joint ; chemistry ; physiopathology ; Young Adult

Acute Disease ; therapy ; Adolescent ; Adult ; Bloodletting ; Child ; Female ; Humans ; Male ; Middle Aged ; Tonsillitis ; therapy ; Young Adult