Objective: To study the chemical constituents in the stems of Zhuang Medicine Alsophila spinulosa. Methods: The chemical constituents were isolated and purified by silica gel, polyamide, and Sephadex LH-20 column chromatography. Their structures were elucidated by physicochemical properties and spectral analyses. Results: Sixteen compounds were isolated from the 50% and 95% ethanol extracts and identified as 30-O-β-D-xylopyranosyl-dryocrassol (1), pimaric acid (2), 9α-hydroxy-1β-methoxycaryolanol (3), 6β-hydroxy-24-ethyl-cholest-4-en-3-one (4), 1-O-hexadecanolenin (5), clovandiol (6), decumbic acid (7), n-tetracosane (8), 4-O-β-D-glucopyranosyl-p-coumaric acid (9), (E)-4-O-β-D-glucopyranosyl caffeic acid (10), cyathenosin A (11), protocatechualdehyde (12), protocatechuic acid (13), stigmastane-3, 6-dione (14), β-sitosterol (15), and daucosterol (16). Conclusion: Compound 1 is a new compound named alspineoside A, and compounds 2-11 are isolated from the stems of A. spinulosa for the first time.

Brain Neoplasms ; pathology ; physiopathology ; DNA Repair ; genetics ; Glioma ; pathology ; physiopathology ; Humans ; Statistics as Topic ; Targeted Gene Repair

Objective: To study the chemical constituents of Gynostema pentaphyllum. Methods: The chemical constituents were isolated and purified by silica gel, polyamide, and Sephadex LH-20 chromatography. Their structures were elucidated by physicochemical properties and spectral analysis. Results: Seventeen compounds were isolated and identified as dodecanoic acid (1), β-sitosterol (2), 3, 3', 5-trihydroxy-4', 7-dimethoxyflavanone (3), 1-2-benzenediol (4), 3'-O-methyltaxifolin (5), quercetin (6), rhamnetin (7), α-spinasterol-3-O-β-D-glucopyranoside (8), 3, 4-dihydroxy benzoic acid (9), narcissoside (10), L-rhamnosemono-hydrate (11), malonic acid (12), β-ethoxy-rutinoside (13), rutin (14), ombuoside (15), ginsenoside Rb1 (16), and β-daucosterol (17). Conclusion: Compound 1, 3-5, 7, 9, 10, and 12 are obtained from G. pentaphyllum for the first time.

The factors impacting the pregnancy rate and the live birth rate mainly include ovary function disorder and low endometrial receptivity, which can cause the difficulty in embryo implantation, early miscarriage and pregnancy failure. In recent years, researchers of the traditional Chinese medicine (TCM) have made active efforts in assisting IVF-ET, so as to achieve a great advance in improving the ovary reaction, treating the ovarian hyperstimulation syndrome (OHSS), improving the follicle, embryo quality and endometrial receptivity and protecting the fetus, which had been summarized in this article.
Drugs, Chinese Herbal ; administration & dosage ; Embryo Transfer ; Female ; Fertilization in Vitro ; drug effects ; Humans ; Infertility, Female ; drug therapy ; therapy ; Pregnancy

Accidents, Occupational ; Carbon Dioxide ; poisoning ; Fatal Outcome ; Humans

Objective That decline of estradiol in serum of postmenopausal women can result in dry eye has been accepted at present. However, the change of estradiol in tear of postmenopausal women has been not reported ever. This study was to discuss the relationship between dry eye syndrome and estradiol in tears and serum of postmenopausal women. Methods Total 62 eyes of 62 women were collected in this trial. The aim of this trial was informed orally and the informed consent was obtained from all the subjects. The 32 right eyes were from menopausal women for more one year and 30 right eyes were from premenopause women. The dry eye was diagnosed based on the abnormalities of tear break-up time (BUT), Schirmer I test, corneal fluorescence and subjective symptom of patients. The periphery blood specimen was collected from all the subjects, and tear specimen was obtained and quantified using filter paper. Estradiol contents in tears and serum were determined by chemiluminescence method. The bulbar conjunctiva was obtained by impression cytology, and the microstructure of conjunctiva was observed under the scanning electron microscopy. Results In comparison to the premenopause group, the content of estradiol in tears and serum in menopause group was significantly reduced(73. 50 ±24.47 vs 17. 43 ± 10. 92) (U = -6. 396, P =0. 001). In the premenopause group, the estradiol content between tear and serum showed the positive correlation (r = 0. 838, P = 0. 001) . However, no statistical correlation was found in estradiol content between tears and serum in menopause group(r =0. 127, P = 0. 487). The maicrostructure changes were seen in the dry eyes of postmenopausal women, and decrease of goblet cell and loosen of intercellular junction were found under the electron microscope. Conclusion The content of estradiol in tears in menopause group is significantly lower than that in the premenopause group. Estradiol content in tears and serum of the postmenopausal women showes insignificant correlation. This result indicates that the topical substitute therapy with estradiol is possible in dry eye.

Objective To patients with neonatal dacryocystitis underwent left Ofloxacin Gel, Tobramycin and Dexamethasone Ophthalmic Ointment and lacrimal surgery combined with treatment effects were observed.MethodsIn Ningxia Hui Autonomous Region Qingtongxia City People's Hospital during December 2015 to December 2016 for dacryocystitis in 130 cases according to the data analysis, using different treatment schemes were divided into two groups, 65 cases underwent lacrimal surgery as control group, 65 cases and left Ofloxacin Gel, Tobramycin and Dexamethasone Ophthalmic Ointment combined treatment as the observation group, blocking characters and comparison of complications two groups of clinical curative effect, lacrimal duct.ResultsIn the observation group, the total effective probability of 96.92% higher than the control group of 80.00%(P<0.05), and the nasal deformity, nasolacrimal duct, lacrimal bone deformity multiple adhesions or obstruction and lower incidence of membranous obstruction of lacrimal duct is lower than that of control group (P<0.05);the total complication rate in the observation group(3.08%) than the control group(21.54%)low (P<0.05).ConclusionNeonatal dacryocystitis underwent left Ofloxacin Gel, Tobramycin and Dexamethasone Ophthalmic Ointment and lacrimal surgery combined therapy can improve the clinical symptoms and characteristics of lacrimal duct obstruction, and reduce postoperative complications, which may be of clinical promotion and application.

Gout and hyperuricemia, the frequently-occurring and common diseases, are mainly characterized by the rise of serum uric acid levels. Overproduction and/or underexcretion of uric acid are the primary causes of hyperurice- mia. As an important organ for uric acid elimination, the kidney is responsible for 2/3 uric acid excretion. Recent researches have shown that urate transporters mediate the urate reabsorption and secretion in proximal kidney tubules. The abnormal expression and functional changes of urate transporters are closely related to the occurrence and develop- ment of hyperuricemia. Recently, studies on the urate transporters have achieved great progresses, including the progress in the studies on the urate-anion transporter 1(URAT1), glucose transporter 9, ABC transporter family, sodium-depen- dent phosphate transport protein 1(NPT1), NPT4, and organic anion transporter family, etc. The roles of organic anion transporters(OAT, such as OAT1, OAT2, OAT3, OAT4, OAT10 and URAT1)in renal uric acid excretion are summa- rized in this review.

Objective: To study chemical constituents of Argyreia acuta. Methods: The chemical constituents were isolated and purified by silica gel, macroporous resin adsorption, MCI GEL CHP 20P, Sephadex LH-20 chromatography, and preparative liquid chromatography. Their structures were identified by physicochemical properties and spectral analysis. Results: Ten compounds were isolated and identified as ethyl caffeate (1), hispidulin (2), quercetin (3), scopolin (4), paprazine (5), mannitol (6), hesperetin (7), ipalbidinium-4’-O-β-D-(6-O-trans-coumaroyl)-glucoside (8), nepitrin (9), and homoplantaginin (10). Conclusion: Compound 8 is a new compound named argyreiacutine, and compounds 2, 3, 6, 7, 9, and 10 are isolated from A. acuta for the first time.

Objective: To establish a method to determine 4 active ingredients in Jindan tablets by HPLC.
Methods: Agilent Eclipse X DB C₁₈ (4.6 mm×250 mm, 5 μm) column was adoped using acetonitrile (phase A) -0.1% phophoric acid solution (phase B) as the mobile phase with gradient program at the flow rate of 1.0 mL·min^-1, the detection wavelength was 270 nm, Column temperature was set at 30 ℃.
Results: The linear ranges of gentiopicrin, polydatin, quercetin and emodin were 7.875-78.75 μg·mL^-1（r=0.999 9）, 6.75-67.50 μg·mL^-1（r=0.999 7）, 7.726-77.26 μg·mL^-1（r=0.999 4）, 3.809-38.09 μg·mL^-1（r=0.999 8), respectively, the peak area had a good linear relationship with the mass concentration of 4 components, the average recovery were 99.31%, 99.21%, 99.04%, 99.59%, (n=6) respectively, and RSDs were 1.86%, 1.24%, 1.37%, 1.15%, respectively.
Conclusion: This method is reliable, repeatable and stable, and has strong specificity. This method can provide a reference for the quality control and standard improvement of Jindan tablets.