Parkinson's disease (PD) is one of the prevalent neurodegenerative diseases in human being, which is characterized by the selective degeneration and death of dopaminergic neurons in the substantia negra. The etiology of PD is not illuminated yet. Currently this disease is thought to be the result of the co-operation of multiple factors. So far, PD can't be cured, and all the treatments are to control its symptoms. DA, PDI, Parkin gene, PINK1 gene and α-synuclein are all related to PD. Apoptosis may play a role in the degeneration and death of dopaminergic neurons. Disfunction of mitochondria appears to play a role in the pathogenesis of PD. The levels of CoQ10 from PD patients have been reported to be lower than that from normal people, and the supplement of CoQ10 favors the assistant treatment of PD.

This paper was aimed to summarize the concept of fuzzy mathematics inZhang Zhongjing’s Treatise on Febrile and Miscellaneous Diseases, in order to refine the accurate dialectical thinking ofZhang Zhongjing in aspects of the theory, method, prescription and medicine for the discussion of precision treatments byZhang Zhongjing. It was aimed to find a breakthrough point from evidence-based medicine (EBM) to precision medicine in traditional Chinese medicine (TCM). It also illustrated TCM by scientific theories. It provided evidences for the inheritance and promotion of TCM as well as methodological references for TCM research.

To investigate the antibiotic resistance of bacteria isolated from bile, the bile from the surgical in-patients with bile duct infection from June 1995 to December 1998 was cultured and the distribution and antibiotic resistance of the isolates (not including anaeobes) were analyzed. The results showed that the cultured positive rate in the specimens was 69. 76 % (143/205). Among the isolates, most of them were E. coli and P. aeruginosa (31. 74 % and 18. 56 %, respectively), while Enterobacter, K. pneumonia and Enterococci were also commonly seen. All the isolates were resistant more or less to the commonly used antibiotics.

Objective To explore the feasibility and the clinical effect of laparoscopic treatment of incomplete small intestinal obstruction.Methods Eighty-five patients with intestinal obstruction from January 2011 to January 2016 were analyzed retrospectively.Five cases caused by adhesion band were released by laparoscopic cut of the adhesion band.Seventy-five cases were caused by adhesion between intestine, abdominal wall, and pelvic cavity were given laparoscopic adhesion and angle separation.Laparoscopic exploration found appendicitis in 3 cases and a laparoscopic appendectomy was carried out.Two cases were given laparoscopic removal of foreign body inside the intestine.For patients with obvious abdominal distention, an indwelling ileus tube was placed in the intestine before the operation.Results All the laparoscopic surgeries were successfully performed except 2 patients required an additional small incision to perform partial enterectomy and intestinal anastomosis due to intensive local adhesion.No complications happened.Postoperative follow-ups for 3-60 months (mean, 35 months) found no recurrence.ConclusionsLaparoscopic treatment of small intestinal obstruction is feasible and minimally invasive, having advantages of small incision, rapid recovery and short hospitalization.It avoids the trauma caused by open operation.

Objective To observe the therapeutic efficacy of Treating chronic hepatitis B with compound BieJiaRuanGan pill plus entacavir. Methods 168 patients were randomly recruited into a treatment group and a control group. The treatment group was treated with compound BieJiaRuanGan pill plus entacavir and the control group was treated with entacavir exclusively, with a course of 48 weeks. Results Both groups showed statistical significance in improving the liver function, peripheral T lymphocyte subsets and indexes of serum liver fibrosis after the treatment (P<0.01 or P<0.05 ). The therapeutic effects in the treatment group were significant better than the control group after the treatment (P<0.01orP< 0.05 ) . Conclusion The treatment of chronic hepatitis B with compound BieJiaRuanGan pill plus entacavir is better than using entacavir exclusively.

Objective To study the mechanism of toxicity of methylmercury on primary cultured SD rat astrocyte.Methods Treat primary cultured SD rat astrocyte with methylmercury.Determine the level of cyto-oxidation redoxin with High Performance Liquid Chromatography(HPLC).Western-blot was applied to examine the protein expression of nuclear factor erythroid 2 related factor 2(Nrf2).Results With the increase of the concentration of methylmercury,the redoxin level of astrocyte was apparently decreasing.When the concentration of methylmercury was at a low level,the expression of Nrf2 was increasing with the increase of the methylmercury concentration.When the concentration of methylmercury was at a high level,the expression of Nrf2 was decreasing with the increase of the methylmercury concentration.Conclusion The toxicity of methylmercury on primary cultured SD rat astrocyte can be realized through altering the function of GSH and Nrf2.

0.05);It was related to the liquid filter press and sealing liquid(P0.05);And the rate of swelling incidence was obviously higher than the phlebitis incidence(P

Our aim in this study was to determine differentially expressed genes of CD34 + cell from bone marrow and G CSF mobilized peripheral blood. CD34 + cells isolated from bone marrow and G CSF mobilized peripheral blood of the same donor were identified for differentially expressed genes in CD34 + cells using the technique of suppression subtractive hybridization. Eleven genes were expressed with higher levels in CD34 + cells from mobilized peripheral blood, as compared with data of GenBank. These genes included nuclear proteins, transcriptional regulatory molecules, zinc finger proteins and interferon induced molecules. These results demonstrate that there are some differences between the two groups of CD34 + cells. Further study would give us more extensive understanding about mobilization and homing of hematopoietic stem cells.

Objective To explore the potential of converting the chloramphenicol resistant clinical E. Coli isolates to chloramphenicol sensitive ones by employing external guide sequence(EGS) technique in vitro. Methods Recombinant plasmids with EGScat 1+2 and tetracycline resistant gene, named PAlterl EGScat1+2, was constructed. Routine Cacl 2 method was used to introduce recombinant plasmid into the chloramphenicol resistant clinical isolates E. Coli 4758. Colony PCR was used to test and A600 was used to detect growth rates in liquid and solid culture of various concentrations of chloramphenicol. Results The chloramphenicol resistant clinical isolates E. Coli 4758 grew well in chloramphenicol(35 ?g/ml, 70 ?g/ml, 105 ?g/ml, 170 ?g/ml) plates whereas the transformants tE4758 with PAlter1 EGScat 1+2 failed to grow in these concentrations, which indicated its resistance to the chloramphenicol was reversed. Conclusions EGS molecules are able to convert the drug resistance in clinical E. Coli isolates in vitro.

Alzheimer's disease(AD),the most common form of dementia,it is lack of effective cure or preventive treatment.Dementias in the elder are an increasing medical,social and economic problems and current treatments are only mildly effective.Recently,amyloid-beta protein(A?) has become a major therapeutic target.A? vaccine treatment can improve cognition in the patients with AD,but adverse events,such as meningencephalitis were observed in clinical study.The passive A? immunotherapy in humans is effective with possible safety.However,patients need to be monitored carefully.