3.FGF-2 combined with TGF-β1 induces bone marrow mesenchyma stem cells differentiating into cardio-myocyte-like cells in vitro
Rou LI ; Haiping WANG ; Yang LV ; Wei SUN ; Xiaoyi CHEN
The Journal of Practical Medicine 2017;33(24):4019-4023
Objective To expore the effects of FGF-2 and TGF-β1 on the differentiation of rat bone mar-row mesenchymal stem cells(BMSCs)towards cardiocytes. Methods BMSCs were isolated from bones of SD rats and cultured and identified by flow cytometry.The cells were divided into four groups:group A(FGF-2),group B (TGF-β1),group C(FGF-2+TGF-β1)and group D(blank control).The morphological changes were observed by the microscope. The expressions of Desmin,α-sarcomeric actin and cTnI were detected by immunocytochemical stainning.The expressions of GATA-4 and Nkx2.5was detected by RT-qPCR.Result The positive expression rate of CD90,CD29 and CD45 was 84.7%,86.5%,0.3% respectively. After induction,group C presence of myotube structures and the shape is fusiform.The positive rate of combined induction group was higher than the others (P < 0.05). The expression of GATA-4 and Nkx2.5 genes was higher than that in the other groups(P < 0.05). Conclution FGF-2 and TGF-β1 can be used to induce BMSCs into cardiocytes,and the combined group has the better effect.
4.Screening and Evaluation of Yeast Antagonists for Biological Control of Botrytis cinerea on Strawberry Fruits.
Pei Hua CHEN ; Rou Yun CHEN ; Jui Yu CHOU
Mycobiology 2018;46(1):33-46
Gray mold (Botrytis cinerea) is one of the most common diseases of strawberries (Fragaria × ananassa Duchesne) worldwide. Although many chemical fungicides are used for controlling the growth of B. cinerea, the risk of the fungus developing chemical resistance together with consumer demand for reducing the use of chemical fungicides have necessitated an alternative method to control this pathogen. Various naturally occurring microbes aggressively attack plant pathogens and benefit plants by suppressing diseases; these microbes are referred to as biocontrol agents. However, screening of potent biocontrol agents is essential for their further development and commercialization. In this study, 24 strains of yeast with antagonistic ability against gray mold were isolated, and the antifungal activity of the volatile and diffusible metabolites was evaluated. Putative mechanisms of action associated with the biocontrol capacity of yeast strains against B. cinerea were studied through in vitro and in vivo assays. The volatile organic compounds produced by the Galactomyces candidum JYC1146 could be useful in the biological control of plant pathogens and therefore are potential alternative fungicides with low environmental impact.
Botrytis*
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Fragaria*
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Fruit*
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Fungi
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In Vitro Techniques
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Mass Screening*
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Methods
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Plants
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Volatile Organic Compounds
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Yeasts*
5.Advance of chemical constituents and bioactivity of Saururuaceae plants.
Lin MA ; Feng WU ; Rou-yun CHEN
China Journal of Chinese Materia Medica 2003;28(3):196-198
Essential oil, flavonoids, alkaloids and lignas were isolated from Saururuaceae plants. They possesses antibacteral, antiviruses, antiinflammatory. Inhibitory of plaleted aggregation activites and exhibit significant neuroleptic activity.
Alkaloids
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chemistry
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isolation & purification
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pharmacology
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Animals
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Anti-Inflammatory Agents, Non-Steroidal
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pharmacology
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Antiviral Agents
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pharmacology
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Drugs, Chinese Herbal
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isolation & purification
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pharmacology
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Lactams
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Oils, Volatile
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isolation & purification
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pharmacology
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Phenanthrenes
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Plants, Medicinal
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chemistry
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Platelet Aggregation Inhibitors
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pharmacology
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Quercetin
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isolation & purification
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pharmacology
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Saururaceae
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chemistry
6.Effect of Dance Intervention on Cognitive Function and Balance Function in Elderly with Mild Cognitive Impairment: A Systematic Review
Chinese Journal of Rehabilitation Theory and Practice 2021;27(12):1430-1436
Objective To explore the effect of dance intervention on the cognitive function and balance function in the elderly with mild cognitive impairment and its possible mechanism through systematic review of related literatures. Methods The literatures of randomized controlled trials on the impact of dance intervention on the cognitive function and balance function of the elderly with mild cognitive impairment were searched in the Cochrane library, PubMed, CBM, Web of Science, EMBASE, CNKI, VIP, Wanfang data and other databases, from establishment to June 7th, 2021. Two researchers screened the literature according to the inclusion and exclusion criteria, extracted data, and evaluated the quality. Results The scores of Mini-Mental State Examination (MMSE) and Montreal Cognitive Assessment (MoCA) were better in the dance intervention experimental group than in the control group, as well as the scores of Wechsler Memory Scale (WMS), Trail Making Test (TMT)-B and Verbal Fluency Test. However, no significant difference was found in the response time in TMT-A between two groups. Dance intervention could improve the scores of Berg Balance Scale. Conclusion Dance intervention can improve overall cognitive function, memory function, executive function and balance function of the elderly with mild cognitive impairment.
7.Trait anxiety and attentional bias in policemen
Rou DONG ; Fei GUO ; Lan JIANG ; Zhiyan CHEN
Chinese Mental Health Journal 2019;33(2):126-131
Objective: To investigate the characteristics of attentional bias in emotional faces of policemen with different trait anxiety levels. Methods: By using the State-Trait Anxiety Inventory (STAI), 44 policemen (23 males and 21 females) were included in the higher trait anxiety level group and 44 policemen (25 males and 19 females) were included in the lower trait anxiety level group. The 2 anxiety style ((higher trait anxiety level, lower trait anxiety level) × 2 emotional type (positive, negative) × 2 clue type (identical side, opposite side) hybrid design was used to investigate the attentional bias of the response time differences between the two groups of policemen on keystroke responses of different emotional types and different types of clues by using a dot probe. Results: The positive emotional face reaction of the higher trait anxiety level group was less than that of the negative emotional face reaction[ (638. 0 ± 12. 4) ms vs. (651. 7 ± 13. 1) ms, P < 0. 01], while the lower trait anxiety level police had no statistical significance on the reaction of positive emotional face and negative emotional face (P> 0. 05). The clue types in the higher trait anxiety level group were identical side less than opposite side response time [ (640. 3 ± 12. 6) ms vs. (649. 5 ± 13. 0) ms, P < 0. 05], and there was no statistical significance in the lower trait anxiety level group about identical side and opposite side response time (P> 0. 05). The clue type in identical side, there was no significant difference in the response of two groups to positive emotional faces and negative emotional faces (P> 0. 05); while the clue type in opposite side, the response of higher trait anxiety level group to negative emotional faces was higher than that of positive emotional faces [ (663. 1 ± 9. 8) ms vs. (651. 4 ± 8. 9) ms, P < 0. 05]. Conclusion: It suggests that the policemen with higher level of trait anxiety have selective attentional bias to negative emotional faces, which induced by impaired attentional disengagement.
8.Does Spore Count Matter in Fungal Allergy?: The Role of Allergenic Fungal Species.
Wan Rou LIN ; Yi Hsing CHEN ; Mey Fann LEE ; Ling Yi HSU ; Chih Jen TIEN ; Feng Ming SHIH ; Shih Ching HSIAO ; Pi Han WANG
Allergy, Asthma & Immunology Research 2016;8(5):404-411
PURPOSE: Fungi have been known to be important aeroallergens for hundreds of years. Most studies have focused on total fungal concentration; however, the concentration of specific allergenic fungi may be more important on an individual basis. METHODS: Ten fungal allergic patients and 2 non-fungal allergic patients were enrolled. The patients with a decrease in physician or patient global assessment by more than 50% of their personal best were considered to have an exacerbation of allergic symptoms and to be in the active stage. Those who maintained their physician and patient global assessment scores at their personal best for more than 3 months were considered to be in the inactive stage. The concentrations of dominant fungi in the patients' houses and outdoors were measured by direct and viable counts at active and inactive stages. RESULTS: The exacerbation of allergic symptoms was not correlated with total fungal spore concentration or the indoor/outdoor ratio (I/O). Specific fungi, such as Cladosporium oxysporum (C. oxyspurum), C. cladosporioides, and Aspergillus niger (A. niger), were found to be significantly higher concentrations in the active stage than in the inactive stage. Presumed allergenic spore concentration threshold levels were 100 CFU/m3 for C. oxysporum, and 10 CFU/m3 for A. niger, Penicillium brevicompactum and Penicillium oxalicum. CONCLUSIONS: The major factor causing exacerbation of allergic symptoms in established fungal allergic patients may be the spore concentration of specific allergenic fungi rather than the total fungal concentration. These results may be useful in making recommendations as regards environmental control for fungal allergic patients.
Aspergillus niger
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Cladosporium
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Colony Count, Microbial*
;
Family Characteristics
;
Fungi
;
Humans
;
Hypersensitivity*
;
Niger
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Penicillium
;
Spores*
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Spores, Fungal
9.Study on evaluation framework of integrated health care system
Zhi-Rou CHEN ; Ying ZHENG ; Tao DAI ; Qing-Bo WANG
Chinese Journal of Health Policy 2018;11(4):29-35
Objective:The aim of this study is to establish an evaluation framework for an integrated health care service system,which provides evidence for the further construction of the evaluation index system. Methods:A sys-tematic review,thematic system and expert consultation were synthetically used to screen,construct and improve the evaluation framework of the integrated health care service system. Results:The results of this study show that the fi-nalized evaluation framework of the integrated health care system contains 4 first-level indexes which include system integration,organizational integration,professional integration and service integration; and 25 corresponding second level indictors. Conclusions:The evaluation framework of the integrated health care service system constructed in this study has a strong effectiveness and suitability, which lays a good foundation for further development of the corre-sponding evaluation index system.
10.Effects of Dance Activity on Negative Emotion in Older Adults: A Systematic Review
Ting-ting CHEN ; Ming-chao XU ; Rou WEN
Chinese Journal of Rehabilitation Theory and Practice 2021;27(6):668-676
Objective:To explore the rehabilitation effects of dance activity on negative emotion in older adults, and clarify the multiple factors that determine the dose-effect of emotional rehabilitation. Methods:The electronic databases of PubMed, Web of Science, EBSCO, Cochrane Library, CNKI, VIP and Wanfang data were searched from inception to December 31, 2020. The authors, countries, years, samples, study designs, interventions, measurement instruments and outcomes were extracted. The PEDro scale was used to assess the methodological quality of included literatures. Results:A total of 672 studies were retuned, out of which twelve were selected including 808 individuals. These studies were published in ten countries, and most in journals related to clinical medicine, exercise rehabilitation, psychotherapy and gerontology. Study designs included randomized controlled trial (RCT), quasi-experimental study and pre-post study. The mean PEDro score was 5.58. There were four types of dance activity, including social dance, dance movement therapy, leisure dance and folk dance. The latter two types were the main rehabilitation interventions. Dance was found to significantly reduce anxiety and depression levels, as well as distress and stress in older adults. Effect of dance activity on negative emotion in older adults was related to intensity, frequency and duration variables, and needed more researches with well-controlled designs, standardized assessments and longitudinal follow-ups. Community was the primary field for the elderly to participate in dance activities. Integrating dance activities into the community rehabilitation service system might contribute to their emotional adjustment and mental health. Conclusion:Dance activity is feasible and effective to alleviate negative emotion, promote psychological healthy and improve quality of life for older adults.