1.Development of an enzyme-linked immunosorbent assay for determination of the furaltadone etabolite, 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) in animal tissues.
Peng Jie LUO ; Wen Xiao JIANG ; Ross C BEIER ; Jian Zhong SHEN ; Hai Yang JIANG ; Hong MIAO ; Yun Feng ZHAO ; Xia CHEN ; Yong Ning WU
Biomedical and Environmental Sciences 2012;25(4):449-457
OBJECTIVETo determine 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) residues released from protein bound AMOZ in animal tissues.
METHODSPolyclonal and monoclonal antibodies were produced in this study. A rapid, sensitive, and specific competitive direct enzyme-linked immunosorbent assay (cdELISA) was developed.
RESULTSRabbit polyclonal antibodies were used in the optimized cdELISA method, and exhibited negligible cross-reactivity with other compounds structurally related to AMOZ. The IC(50) of the polyclonal antibody was 0.16 ng/mL. The method limit of detection in four different types of animal and fish tissues was less than 0.06 μg/kg. Recoveries ranged from 80% to 120% for fortified samples with the coefficient of variation values less than 15%. The results of the cdELISA method were in good agreement with the results from an established liquid chromatography-tandem mass spectrometry confirmatory method used for AMOZ residues.
CONCLUSIONThe cdELISA method developed in the present study is a convenient practical tool for screening large numbers of animal and fish tissue samples for the the detection of released protein bound AMOZ residues.
Animals ; Enzyme-Linked Immunosorbent Assay ; methods ; Molecular Structure ; Morpholines ; analysis ; chemistry ; Nitrofurans ; analysis ; chemistry ; Oxazolidinones ; analysis ; chemistry
Result Analysis
Print
Save
E-mail