Bone Neoplasms ; immunology ; pathology ; Child ; Humans ; Osteosarcoma ; immunology ; pathology ; Rosette Formation

Eccrine spiradenoma is a rare adnexal tumor of the skin and its cytomorphology has seldom been described in literature. To evaluate the cytologic features and diagnostic pitfalls of eccrine spiradenoma, we report a case of 33-year-old male whose clinical presentation was a painful subcutaneous mass at the right preauricular area. Fine needle aspirates revealed tight clusters of multilayered, uniform, cuboidal cells arranged around hyalinized perivascular spaces. Some epithelial cells showed tubules or rosette formations. The background showed scattered, naked nuclei. The histologic findings confirmed the eccrine spiradenoma. The recognition of peculiar cytologic features and clinical correlation could be helpful in the diagnosis of skin adnexal tumors.
Adult ; Biopsy, Fine-Needle* ; Diagnosis ; Epithelial Cells ; Humans ; Hyalin ; Male ; Needles ; Rosette Formation ; Skin

OBJECTIVETo study the changes of red cell immune function and T-lymphocyte subsets in children with bronchiolitis and their possible roles in the pathogenesis of bronchiolitis.

METHODSForty-five children with bronchiolitis and 30 healthy controls were enrolled. Red cell immune complex rosette (RBC-ICR) and red cell C3b receptor rosette (RBC-C3bRR) were detected. The percentages of CD3+, CD4+ and CD8+ cells were assayed by flow cytometry.

RESULTSRBC-C3bRR［(13.6 ± 6.2)% vs (18.0 ± 7.4)%] and the percentage of CD8+ cells [(21.6 ± 4.4)% vs (25.6 ± 5.2) %］ in the bronchiolitis group were lower than those in the control group (P<0.01). The percentage of CD3+ cells ［(59.9 ± 6.7)% vs (52.1 ± 8.3)%］ and CD4+ cells [(53.5 ± 6.2)% vs (46.8 ± 4.9)%] and RBC-ICR [(8.3 ± 3.5)% vs (6.1 ± 2.5)%] in the bronchiolitis group were higher than those in the control group (P<0.01). The percentage of CD4+ cells was positively correlated with RBC-ICR (r=0.63,P<0.05) and negatively correlated with RBC-C3bRR (r=-0.82,P<0.01).

CONCLUSIONSThere are dysfunctions of red cell immune and T-lymphocyte subsets in children with brochiolitis, which may play a role in the pathogenesis of brochiolitis.

Bronchiolitis ; etiology ; immunology ; Erythrocytes ; immunology ; Female ; Humans ; Infant ; Male ; Receptors, Complement 3b ; physiology ; Rosette Formation ; T-Lymphocyte Subsets ; immunology

Primitive neuroectodermal tumor (PNET), also referred to as extraskeletal Ewing's sarcoma, is a highly aggressive tumor, and it is seen typically in the soft tissues of the chest wall and paraspinal region but rarely arises in the kidney. A 27-year-old woman was presented with a left renal mass and underwent a radical nephrectomy. The pathological diagnosis was a primitive neuroectodermal tumor. Microscopically, there were sheets and lobules of cells with round to oval nuclei and coarse chromatin, as well as a Homer Wright rosette formation. Immunohistochemical analysis showed strongly positive staining of CD99 and negative staining of other neuroendocrine markers, such as synaptophysin and chromogranin.
Adult ; Chromatin ; Diagnosis ; Female ; Humans ; Immunotherapy ; Kidney* ; Negative Staining ; Nephrectomy ; Neuroectodermal Tumors, Primitive* ; Rosette Formation ; Sarcoma, Ewing ; Synaptophysin ; Thoracic Wall

The 51 cases of eye tumors were evaluated histologically from May 1980 to August 1987 at Department of Ophthalmology, Catholic University Medical College, Kangnam St. Mary's Hospital. The results were as follows: 1. The ratio of male to female among 51 cases was 1:1.04 and histologically benign tumors 25 cases(49%), malignant tumor 26 cases(51%) respectively. 2. Histopathologic type of the most common benign tumor is dermoid cyst(9 cases, 36%), the next nevi(4 cases, 16%) and the third mixed tumor(3 cases, 12%). Among malignant tumor, retinoblastoma(17 cases, 65.5%) was most common and malignant melanoma(5 cases, 19.3%) follows next. 3. In case of benign tumor, there was almost no occurring differences in each age group but in malignant tumor, it showed the high incidence prior to 9 years of age and after 40 years of age. 4. The locations of tumor in benign tumor were conjunctiva, eyelid, lacrimal apparatus in erder. The most common location in malignant tumor was retina. 5. Looking at histopathologic findings of retinoblastoma, there were endophytic types(13 cases, 76.5%) and exophytic types(4 cases, 23.5%). There were 10 cases(52.5%) of optic nerve invasion and 3 cases among total 4 cases of exophytic types. And also, only one case of optic nerve invasion occurred in cases of rosette formation.
Conjunctiva ; Dermoid Cyst ; Eyelids ; Female ; Humans ; Incidence ; Lacrimal Apparatus ; Male ; Ophthalmology ; Optic Nerve ; Orbit* ; Retina ; Retinoblastoma ; Rosette Formation

We report a case of neuroblastoma with multiple skin metastases as a chief complaint in a 2-month-old girl. the skin lesions were rnultiple, pea-sized, bluish, nontender, moable subcutaneous nodules on abdomen, back and scalp. Histopathology showed small round or poly gonal tumor cells which have deeply stained, basophilic, hyperchromatic nuclei with some mitoses. Th.se tumor cells showed clumping tendency which is one of early menifestations of rosette formation. Immunohistochemically positive reaction was demonstrated by anti-NSE(neuron specific enolase) antilody but negative reaction by anti-NFP (neurofilament proteiin ) antibody. She has been succesfully treated with combined chemotherapy for 10 months without relapse.
Abdomen ; Basophils ; Drug Therapy ; Female ; Humans ; Infant ; Mitosis ; Neoplasm Metastasis* ; Neuroblastoma* ; Phosphopyruvate Hydratase ; Recurrence ; Rosette Formation ; Scalp ; Skin*

The major categories of neuroendocrine tumors of lung are typical carcinoid, atypical carcinoid, large cell neuroendocrine carcinoma, and small cell carcinoma. The histologic classification criteria of neuroendocrine tumors are well documented in the "WHO Classification of Tumors" based on mitotic figures and necrosis. Cytologic characteristics of neuroendocrine tumors are trabecular, acinar, and solid arrangement of tumor cells and occasional rosette formation. Nuclear chromatin patterns are characteristically described as "salt and pepper chromatin pattern". Many of cytologic classifications documented in the literature are before the "WHO Classification". In this review, the cytologic features of pulmonary neuroendocrine tumors are documented according to the WHO classification, and recent concepts of neuroendocrine tumors of lung are discussed.
Biopsy ; Biopsy, Fine-Needle ; Carcinoid Tumor ; Carcinoma, Neuroendocrine ; Carcinoma, Small Cell ; Chromatin ; Lung ; Lung Neoplasms ; Necrosis ; Neuroendocrine Tumors ; Rosette Formation

The functional immaturity of PMNs is one of the major causes of overwhelming sepsis in newborns. In this study, we observed functions and surface markers of PMNs to investigate what causes the functional immaturity of PMNs in newborns. As results, the percentage of EA rosette forming PMNs (58.5 +/- 15.5%) and the chemotactic movement (0.14 +/- 0.09 mm) of cord blood PMNs were significantly lower than those of adult peripheral blood PMNs (70.8 +/- 9.9%, 0.60 +/- 0.34 mm). Cord blood PMNs showed decreased glass adherence and ADCC activity. The expression of Fc gamma RII or Fc gamma RIII was a little lower than those of adult peripheral blood PMNs, but the expression of Fc gamma RI (43.1 +/- 26.8%) was significantly higher than that of adult peripheral blood PMNs (3.2 +/- 1.8%). There was a significant difference in LFA-1 expression between EA rosette forming PMNs (92.9 +/- 9.1%) and EA rosette non-forming PMNs (25.6 +/- 22.6%). From these results, it is assumed that neonatal PMNs may consist of heterogeneous populations. And the relatively high percentage of EA rosette non-forming PMNs which express a low level of LFA-1 may be responsible for the functional immaturity of cord blood PMNs.
Antibody-Dependent Cell Cytotoxicity ; Cell Adhesion ; Chemotaxis, Leukocyte ; Fetal Blood/*cytology ; Human ; Lymphocyte Function-Associated Antigen-1/*physiology ; Neutrophils/*physiology ; Receptors, IgG/*physiology ; Rosette Formation

We present the cytologic features of small cell neuroendocrine carcinoma of the liver metastasized from the uterine cervix. Cytologically, tumor cells were arranged in a pat- tern of solid sheet in necrotic background. The tumor cells were characterized by uniform, small cells, round hyperchromatic nuclei, and high nuclear cytoplasmic ratio. The smears showed frequent mitotic figures and rosette formation, These findings were identified with the previous histologic sections of uterine cervix. To make a diagnosis of metastatic small cell neuroendocrine carcinoma on the Papanicolaou smear, a high index of suspicion and careful review of clinical history are needed
Animals ; Carcinoma, Neuroendocrine ; Cervix Uteri ; Charadriiformes ; Cytoplasm ; Diagnosis ; Familial Hypophosphatemic Rickets* ; Female ; Fibrous Dysplasia, Polyostotic* ; Liver ; Papanicolaou Test ; Rosette Formation ; Vitamin D* ; Vitamins*

OBJECTIVETo study the correlation of erythrocyte immune function between normal neonates and their mothers and the influence of various obstetric factors on neonatal erythrocyte immune function.

METHODSThe adherent rate of complement 3b-receptor on the surface of red blood cells (RBC-C3bRR) and the immune complex adherent rate of red blood cells (RBC-ICR) were detected using the erythrocyte saccharomyces rosette test in 104 normal neonates and their mothers. The correlation of erythrocyte immune function between neonates and their mothers was evaluated by the maternal-infant paired test.

RESULTSThe levels of RBC-C3bRR (16.80 +/- 1.56% vs 16.23 +/- 1.63%; P < 0.05) and RBC-ICR (5.72 +/- 1.63% vs 5.02 +/- 1.38%; P < 0.01) in neonates were significantly higher than those in their mothers. There was a significantly positive correlation in RBC-ICR levels between neonates and their mothers (r = 0.28, P < 0.05). No correlation was found in RBC-C3bRR levels between the two groups. Neither RBC-C3bRR nor RBC-ICR levels of neonates were associated with various obstetric factors such as amniotic fluid, placenta, umbilical cord, parturient patterns, and puerperal anemia and pregnancy-induced hypertension syndrome.

CONCLUSIONSThe erythrocyte immune function in neonates has a relatively mature level and correlates with their mothers' erythrocyte immune function. Various obstetric factors have no influences on neonatal erythrocyte immune function.

Antigen-Antibody Complex ; immunology ; Erythrocytes ; immunology ; Female ; Fetal Blood ; immunology ; Humans ; Infant, Newborn ; immunology ; Linear Models ; Male ; Pregnancy ; Receptors, Complement 3b ; analysis ; Rosette Formation