23 freshly removed breast tumors without any therapy were studied. Argyrophlilic nuclcolar organizer region proteins (AgNORs) were observed by frozen sections and imprints and the distribution and shape of some AgNORs were analyzed by means of electron microscope. It was found that AgNORs in benign lesions showed regular dotted granules and mean individual counts were significantly fewer (3.33?1.19) than that (9.21?1.24) ( P

The renal histopathological findings of the 71 cases with complete clinical histories were observed, 40 cases developed clinically hepatorenal syndrome, including 11 cases with functional renal failure and 29 cases with acute tubular necrosis.Moreover the former was liable to convert into the latter in coures of time.It was suggested that hepatorenal syndrome should be defined as a complication of advanced liver diseases such as extensive liver cirrhosis, hepatoma, fulminant heptitis,etc.,characterised by acute renal failure with oliguria and azotemia, which pathologically may be shown as functional renal failure or acute tubular necrosis.This paper discussed the pathogenesis of functional renal failure and acute tubular necrosis, the relationship between renal failure and histologic changes of kidneys, as well as the prognosis of hepatorenal syndrome.

By histochemical methods of AKPase, ACPase, 5'-Nase, NSE and SDH, 10 cases of human hepatocellular carcinoma (HCC) and its surrounding liver tissue were investigated. Generally, these enzyme activities in HCCs obviously decreased or completely disappeared, neverthless in one case, the AKPase of HCC showed notable activity, and in another case, the ACPase activity also marked.The surrounding liver tissue either in cirrhosis or in hepatic fibrosis appeared normal hepatic enzymatic activity. The differences of enzymes between HCCs and their surrounding liver tissues were distinct.

This study was designed to investigate the relationship between the hepatotoxicity of Halothane and altered hepatic calcium homeostasis in enzyme-induced hypoxic rats by means of Ca2+ cytochemistry location and EDX microanalysis combined with micropathological stereology of liver and ultrastructural analysis by computer. The results showed that more calcium precipitated the cytoplasm and mitochondria of liver following Halothane exposure than the controls. Based on the experiments, this alteration appeared to be contributed to the accelerated uptake of Ca2+ by the hepatocytes and lessened to pump out the decreased loaded Ca2+ on the other hand. The release of the reserved Ca2+ was increased by Halothane as compared with controls. These results suggest that a loss of regulation of cellular Ca2+ levels plays an important role in the mechanism of hepatotoxicity by Halothane exposure.

0.05). There was no difference between various historical types neither. But it was found that there was a trend that AgNOR counts would increase as the increase of the historical grade and there was a significant difference (P

Objective: To study the expression of CD15 mRNA,CD44v6 mRNA and nm23H1 mRNA in colon carcinoma and its relationship with clinical pathological parameters and prognosis of colon carcinoma. Methods: In situ hybridization was used to detect the expression of CD15 mRNA, CD44v6 mRNA and nm23H1 mRNA in 90 cases of colon carcinoma, and 53 cases were followed up for more than 5 years. Results: In 90 cases of colon carcinoma, the expression of CD15 mRNA,CD44v6 mRNA and nm23H1 mRNA were 84.4%,68.9%,66.7%, respectively. The high level expression of CD15 mRNA, CD44v6 mRNA and the low level expression of nm23H1 mRNA were positively corelated with the Dukes staging,serosa infiltration,lymph node and liver metastasis of colon carcinoma.The expression of CD15 mRNA in colon carcinoma was positively associated with that of CD44v6 mRNA and negatively with that of nm23H1 mRNA. Conclusion: CD15 mRNA,CD44v6 mRNA and nm23H1 mRNA play an up-regulation and a down-regulation role, respectively, and have a synergistic action in metastasis of colon carcinoma. CD15 mRNA can be used as a new biological index to predict the invasive potential of colon carcinoma and objectively evaluate the prognosis of patients.

Nuclear DNA content in 103 cases of malignant and benign breast tumours were determined by means of flow cytometry. It was found that DNA in all the benign lesions showed diploids. In contrast, only 32.3 percent of the cancers had diploids, while 62.3% had additional DNA aneuploids including near-diploids (18.4%); triploids (22.3%); and tetraploids (8.2%) etc. Both the cellular proportion in S-phase and DNA index were significantly higher in malignant breast tumours than those in benign ones (P0.05). The results indicate that the analysis of DNA content is a useful and objective adjunct in the assessment of proliferative activity and biological behavior on breast tumour.

Formalin-fixed paraffin-embedded biopsy specimens of the normal nasal mucosa,nasal polyps, inverted papillomas and papillary adenocarcinomas were analyzed by Avidin Biotin-Peroxidase Complextechnique for the demonstration of lectin receptors including peanut agglutinin (PNA), concanavalin ensifomis agglutinin (ConA),ulex europeaus agglutinin-I (UEA-1), sophora japonica agglutinin (SJA), wheat germ agglutinin (WGA), pisum sativum agglutinin (PSA), carcinoembryonic antigen (CEA) and keratin.lt was found that the distribution of PNA and UEA-I receptors was related to the degree of differentiation, dysplasia or malignant transformation of the nasal mucosa. The quantity of ConA receptors was increased with the degree of transformation of the nasal mucosal cells. There were many kinds of lectin receptors on the surface of one kind of cells. Lectin histochemistry was more economical, convenient, rapid and sensitive than immunohistochemistry. So, lectin histochemistry can be used to examine the degree of differentiation, dysplasia and malignant transformation of nasal neoplastic cells, providing objective index for clinical diagnosis, treatment and prognosis.

Objective To explore the guidance significance of the functional MRI and DTI (fMRI ,DTI) ,intraoperative ultra-sound(IOUS) ,neuronavigation ,electrocorticography(EcoG) monitoring used in surgical treatment of low-grade gliomas with epi-lepsy as main symptom located near the eloquent brain regions .Methods 23 neurosurgical patients in the First Affiliated Hospital of Chongqing Medical University during 2009-2010 were performed the retrospective analysis .The preoperative fMRI ,DTI deter-mined the positional relation between the lesions with the conduction bundle and the eloquent brain regions ,the electrophysiological and imageological examinations positioned the epileptogenic focus and lesions ,the MRI-mediated neuronavigation system was adopt-ed to formulate the surgical plan and choose the best surgical approach ,IOUS was used to perform the realtime monitoring for pre-cisely positioning the lesion range and determining the extent of resection ,and the intraoperative EcoG was adopted to determine the epileptogenic focus localization ,the lesions and the epileptogenic focus was dealed by the operating microscope for avoiding the func-tional region ,and the patient′s prognosis was evaluated and recored in detail after operation .Results By the precisely positioning the lesions and epileptogenic focus by fMRI ,DTI ,neuronavigation and ultrasound ,the lesion resection degrees by the operative mi-croscope and intraopertaive pathological guidance were 17 cases of Ⅰ-Ⅱ grade ,4 cases of Ⅲ grade and 2 case of Ⅳ-Ⅴ grade .1 case of motor aphasia ,4 cases of hemiplegia and monoplegia and 1 case of disturbance of consciousness after operation were improved by the treatment of neurotrophy ,dehydration and hyperbaric oxygen and discharged from hospital with rehabilitation .No death case occurred .The evaluation of the life quality :20 cases ofⅠ-Ⅱ grade ,3 cases of Ⅲ grade and no vegetable survival case of Ⅳ grade . The evaluation of resection clinical effect :20 cases of cure ,3 cases of improvement ,no case of as before and exacerbation .After fol-lowed up for 6-24 months ,according to Engel classification of seizure efficacy assessment :Ⅰ-Ⅱ grade in 21 cases ,Ⅲ grade in 2 case ,no case of Ⅳgrade .Conclusion fMRI ,DTI ,neuronavigation ,IOUS and EcoG for guiding the operation of low grade gliomas located near the eloquent brain regions can resect the lesion to the largest extent and simultaneously deal with epileptogenic focus , effectively protect the neurological function of the functional region ,greatly reduce the side-injury of the normal brain tissues in the functional region ,at the same time increase the curative effect of symptomatic epilepsy .