1.Studies on quality standard for Quzhi Capsules
Rongzhen PENG ; Yi WU ;
Chinese Traditional Patent Medicine 1992;0(07):-
AIM: To establish the method for quality control of Quzhi Capsules(Radix et Rhizoma Rhei, Semen Cassiae, Herba Artemisiae Scopariae, Rhizoma Atractylodis, etc.). METHODS: Herba Artemisiae and Rhizoma Atractylodis in this medicine were identified by TLC. The content of chrysophanol was determined by HPLC on ODS column. The mobile phase was composed of methanol 0.5% phosphoric acid(85∶15,v∶v) and detection wavelength was at 254nm. RESULTS: The spots on TLC plates were clear without interference in the blank reference. The linearity of HPLC was good( r =0.9999), and the average recovery was 101.00%, RSD =1.3%. CONCLUSION: This method is simple, sensitive and accurate, and available for control of Quzhi Capsules.
2.Apoptosis induced by berbamine in K562 cells correlates with the expression levels of bcr/abl gene and P210
Dong WU ; Maofang LIN ; Xiaoying ZHAO ; Rongzhen XU
Chinese Journal of Pathophysiology 2000;0(08):-
AIM: To explore the effect of berbamine(BER) on apoptosis in K562 cells and its possible molecular mechanisms. METHODS: The apoptosis rate was measured by flow cytometry while electron microscopy and DNA electrophoresis were used to evaluate the characteristic changes of apoptosis, RT-PCR and Western blot were used to examine the expression levels of apoptosis related gene bcr/abl and BCR/ABL protein. RESULTS: By FCM, the apoptosis rate of K562 cells treated with 8.0 mg/L BER for 24 h and 72 h increased from (29.20?3.82)% to (61.77?4.35)% (P
3.Determination of Ferulic Acid in Bogu Pill by HPLC
Qixun TANG ; Yanling LI ; Rongzhen PENG ; Yi WU
Traditional Chinese Drug Research & Clinical Pharmacology 2000;0(05):-
Objective To establish a method for the content determination of ferulic acid in Bogu Pill(BP). Methods Ferulic acid was obtained by ultrasonic extraction at room temperature and detected by HPLC. HPLC was performed with ODS chromatographic column, methanol-acetonitrile-1%acetic acid(15: 15: 70) as mobile phase and detection wavelength at 323 nm. Results The linearity of ferulic acid was in the range of 0.56 ?g~11.20 ?g/mL(r=0.9996), and the average recovery was 100.26%, RSD=1.48%. Conclusion This method was efficient and can be used for the quality control of BP.
4.Application of random amplified polymorphism DNA technique in systematic classification of origin fungi of Chinese traditional medicine Red Kojic
Heming MI ; Wangxing XING ; Rongzhen CHENG ; Xiang HE ; Yutian WU ;
Academic Journal of Second Military Medical University 2000;0(10):-
Objective:To establish a random amplified polymorphism DNA(RAPD) method for classification of origin fungi of the Chinese traditional medicine Red Kojic. Methods:The genome DNA of 7 ordinary species Monascus including M. purpureus and a strain of Aspergillus terreus were extracted by CTAB. The content of DNA was assayed doubly by fluorescence intensity of ethidium bromide (EB) and spectrometer. Amplification products were detected by agarose gel electrophoresis and clustering analysis by PHYLIP 3.5c. Results:A characteristic pattern was produced depend on one of the 10 primers screened from 60 random primers, and the genome was amplified. The diversities of the fingerprint patterns of Monascus was obvious, by which Monascus can be distinguished easily. The results were consistent with that of morphologic study. Conclusion:RAPD is a new assay technique. It can be used to classify and identify of Monascus fungi. [
5.Role of immune response and inflammatory injury in the pathogenesis of liver failure
Tingshuai WANG ; Na WANG ; Rongzhen ZHANG ; Minggang WANG ; Shaodong HUANG ; Yuzhen MA ; Cong WU ; Dewen MAO
Journal of Clinical Hepatology 2020;36(6):1415-1419
Liver failure is a syndrome of severe liver diseases commonly seen in clinical practice, and it has a high mortality rate and thus becomes one of the critical diseases in internal medicine. Massive hepatocyte death and the extent of hepatocyte death exceeding the liver’s regenerative capacity are considered the core events in the development and progression of liver failure, and direct injury and immune-mediated inflammatory injury are the two main factors in this process. An increasing number of evidence has shown that host immune response and inflammatory cascade play an important role in the pathogenesis of liver failure. This article reviews the mechanism of action of immunoregulation (congenital and adaptive) and inflammatory injury (inflammation inducers, receptor cells, and inflammatory mediators) in the process of hepatic failure, as well as the interactions between immune response and immune cells and between inflammatory response and inflammatory factors, in order to help understand the pathogenesis of liver failure and provide new ideas for the diagnosis and treatment of liver failure and drug research and development.
6.Expression and biological function of TRP signaling pathway in hepatocellular carcinoma
Zhipeng WU ; Yuqin ZHANG ; Minggang WANG ; Rongzhen ZHANG ; Dewen MAO
The Journal of Practical Medicine 2024;40(6):743-747
In recent years,the morbidity and mortality of hepatocellular carcinoma(HCC)have been increasing worldwide,and the treatment strategies for HCC are still insufficient,which highlights the importance of exploring the pathogenesis and progression of HCC.Transient receptor potential(TRP)pathway is an important non-selective cation pathway,which is closely related to inflammatory response and sensory conduction.At present,a number of studies have shown that TRP pathway is also involved in the occurrence and development of HCC,inducing HCC invasion and migration.However,the overall potential mechanism and possible signal transduction pathways of TRP pathway in HCC remain unclear.Therefore,this article discusses the abnormal expression of TRP pathway in HCC,and reviews the key biological events of TRP pathway involved in the formation and progression of HCC,such as chronic liver inflammation-fibrosis progression,HCC cell proliferation,migration,apoptosis and HCC stem cell generation,and looks forward to its application prospect in HCC treatment.The aim is to better un-derstand the significance of TRP pathway in HCC,help to find new therapeutic targets and effective drugs,and open up a new situation for future clinical treatment.
7.Drug resistance and genetic background of New Delhi metallo-β-lactamase ( NDM )-producing bacterial strains in Lishui area of Zhejiang province
Jiansheng HUANG ; Jinglu YU ; Yan ZHU ; Xiaolei HU ; Jianmin REN ; Yunan ZHAO ; Hui DING ; Rongzhen WU ; Zhigang ZHAO
Chinese Journal of Clinical Infectious Diseases 2018;11(4):287-293
Objective To analyze the drug resistance phenotype and genetic background of New Delhi metallo-β-lactamase (NDM)-producing bacterial strains in Lishui area of Zhejiang province.Methods The imipenem-resistant Enterobacteriaceae strains were isolated from January 2012 to December 2016 in Lishui Municipal Central Hospital of Zhejiang Province.Mrieux Vitek 2 Compact system was used to identified strains and PCR was used to screen for blaNDMgene.Susceptibility was detected by K-B method and MICs were obtained by Vitek 2 with GN13 cards.Plasmids typing was carried out by DNA sequencing of the replication initiator with the transconjugates as templates.The blaNDMgenetic contexts were detected by PCR and complete genome sequencing.Results A total of 102 strains of carbapenem-resistant enterobacteria (CRE), mainly Escherichia coli and Enterobacter cloacae, were isolated, of which 15 were positive for blaNDMwith a positive detection rate of 14.7%.The resistance rate to β-lactam antibiotics was 100%, and the resistance rates to aztreonam, compound sulfamethoxazole , tobramycin and gentamicin were all >80%;and the resistance rate to quinolones was >50%.Among the 15 NDM-producing strains , 12 strains were positive for Hodge test, and 2 strains of Enterobacter cloacae and 1 strain of Escherichia coli were negative. There were 11 strains of blaNDM-1and 4 strains of blaNDM-1.A variety of plasmid types such as IncX 3, IncFIIγ and IncA/C were detected, and 4 blaNDM-5genes were located in the IncX3 plasmid.Four blaNDMsurrounding gene structures were found, of which 8 blaNDM-1genes were located in ISAb125-hyp-blaNDM-1-bleMBL-TrpF-DsbC-IS26, while blaNDM-5was located in IS3000-IS5-blaNDM-5-bleMBL-TrpF-DsbC-IS26 structure, and the former was reported for the first time in China.Conclusion NDM-producing bacterial strains in Lishui area are prevalent at a low level and have high sensitivity to fosfomycin and polymyxin .The blaNDMgene may have multiple sources, but IncX3 plasmid is still the main source for gene transfer.Some new types of blaNDM-1 gene structure have been also found in this study.
8. Molecular epidemiology of Klebsiella pneumoniae bla KPC gene in Lishui from 2010 to 2016
Jiansheng HUANG ; Hui DING ; Yang SHI ; Yun′an ZHAO ; Rongzhen WU ; Zhigang ZHAO
Chinese Journal of Microbiology and Immunology 2018;38(8):616-622
Objective:
To investigate the distribution of
9.Analysis of Medication Rules of TCM Prescription against Primary Hepatic Carcinoma Based on TCM Data Analysis Platform Based on Strategy Pattern
Shumao PAN ; Xinyou ZHANG ; Diyao WU ; Yongkun GUO ; Liang DING ; Rongzhen TAN
China Pharmacy 2020;31(24):2966-2973
OBJECTIVE:To estab lish“TCM Data Analysis Platform Based on Strategy Pattern ”(TCMDP),and to analyze the medication rules of TCM prescription against primary hepatic carcinoma (PHC),so as to provide reference for TCM prescription screening. METHODS :According to the idea of strategy pattern ,TCMDP software was developed ,and the frequency statistics,association,clustering,classification and other methods and the different algorithms covered were encapsulated. Effective TCM prescriptions against PHC were collected and screened from 4 tertiary-grade class-A hospitals in Nanchang city and CNKI , PubMed. A database of prescriptions for PHC (PDOPHC)was established ,and TCM prescriptions in the data base were put into TCMDP. Clustering algorithm modified by CMC-DD ,modified WD-Get Rules algorithm after merging depth and width search and frequency statistics were used to analyze the distribution of prescription syndrome. Taking phlegm-blood stasis syndrome type (TYPHC)as example ,data mining was carried out on drug efficacy category ,four properties ,five flavors and channel tropism , drug property combination ,key couplet medicines and key drug combination. RESULTS :A total of 907 TCM prescriptions against PHC were collected ,mainly involving 10 syndrome types ,such as phlegm-blood stasis syndrome ,deficiency of the vital essential and blood stasis syndrome and liver and spleen blood stasis syndrome. TCM related to TYPHC commonly used in pre- scriptions were tonifying deficiency medicine , phlegm-re- solving medicine ,promoting blood circulation and removing blood stasi s medicine ,etc.Four properties were mostly cold ,warm and calm ;the five flavors were most ly swe et,bitter and pungent ;channel tropism were mostly spleen ,liver,stomach, lung,heart and kidney ;drug combination were mostly warming-sweet-spleen ,warming-pungent-spleen and cold-bitter-liver ,etc.; there were 36 couplet medicines with frequency ≥30 times;there were 31 strong association rules and 8 key drug combinations in the prescriptions. WD-Get Rules results showed that in the TCM prescriptions against PHC ,Atractylodes macrocephala ,Codonopsis pilosula,Poria cocos and Glycyrrhiza uralensis were in the key position. They were often combined with many kinds of drugs , which were related to the effect of Sijunzi decoction on strengthening Qi and tonifying spleen. CMC-DD results showed that therapy for TYPHC focused on strengthening the spleen and stomach ,replenishing Qi and blood ;and at the same time ,it treated TYPHC with phlegm-blood stasis syndrome from multiple angles ,such as soothing the liver and regulating Qi ,eliminating phlegm ,clearing heat and detoxification ,promoting blood circulation and nourishing Yin. CONCLUSIONS :Clinical treatment of TYPHC mainly adopts the methods of invigorating the spleen and replenishing Qi ,soothing the liver and regulating Qi ,promoting blood circulation and removing blood stasis ,which conforms to the medication principle of “strengthening the body and eliminating pathogenic factors”. The application of TCMDP can realize the data mining and analysis of TCM in multi-level and multi-directional way , which provides a new and effective way for the mining and research of compatibility rules of TCM prescriptions.
10.Immunotherapy for acute-on-chronic liver failure
Xiaobin QIN ; Rongzhen ZHANG ; Cong WU ; Shenglan ZENG ; Yingyu LE ; Dewen MAO
Journal of Clinical Hepatology 2021;37(11):2696-2700
Acute-on-chronic liver failure (ACLF) is a life-threatening disease with a high risk of multiple organ failure, sepsis, and death. ACLF activates innate and acquired immune responses in human body and thus leads to the progression of persistent systemic inflammatory response syndrome and multiple organ dysfunction, leading to the high mortality rate of this disease. Dysregulated immune response plays a key role in disease progression, and immunotherapy may help to target immune-mediated organ damage and inhibit the progression of liver failure. This article reviews the role and mechanism of drugs and means with a potential immune regulatory effect in ACLF, in order to provide a reference for immunotherapy for ACLF.