Objective To observe the effects of limited fluid resuscitation (LFR) on immune function and inflammatory mediators in patients with multiple bone fracturescomplicated with traumatic hemorrhagic shock. Methods One hundred and two patients with multiple bone fractures complicated with traumatic hemorrhagic shock admitted to the Affiliated Hospital of Medical College of Ningbo University from January 2014 to June 2016 were enrolled, and they were divided into a LFR group and a early sufficient routine fluid resuscitation (RFR) group by random number table, each group 51 cases. After admission, the patients in the two groups underwent bandaging and hemostasis at the traumatic sites and preoperative management was prepared. The patients in RFR group were treated with early sufficient RFR, while LFR group was treated with LFR. The changes of hematocrit (HCT), blood platelet count (PLT), prothrombin time (PT), blood lactic acid and immune cells, inflammatory mediators and micro RNA-155 (miR-155) were observed in patients of the two groups at 4 hours after resuscitation.Results Compared with RFR group, the resuscitation time (hours: 3.67±1.45 vs. 5.14±1.61), levels of PT (s: 11.43±2.21 vs. 15.73±2.52), serum lactic acid (mmol/L: 3.35±0.15 vs. 3.81±0.25), tumour necrosis factor-α [TNF-α (ng/L): 14.10±3.39 vs. 16.28±3.47], interleukin [IL-10 (ng/L): 31.43±10.51 vs. 40.09±13.23, IL-6 (ng/L): 490.10±55.13 vs. 610.30±63.15] and endothelin-1 [ET-1 (pg/L): 183.35±30.51 vs. 250.01±31.23] in LFR group were significantly decreased (allP < 0.01), while PLT (×109/L: 134.58±28.13 vs. 108.12±30.35), HCT (×10-2: 0.34±0.04 vs. 0.24±0.05), miR-155 (0.15±0.02 vs. 0.08±0.02) and CD4+CD25+ regulatory T cell [CD4+CD25+Treg (×10-2): 2.28±0.47 vs. 2.10±0.39] in LFR group were obviously increased (allP < 0.01).Conclusions Using LFR in the emergency treatment of patients with multiple bone fractures complicated with traumatic hemorrhagic shock can effectively shorten the resuscitation time, regulate the patients' coagulation function, reduce the unnecessary excessive liquid infusion, improve immune status and decrease the degree of inflammatory reaction.

This study aimed at investigating the inhibitory effects and the anti-tumor mechanisms of co-adminis-tration of fusion proteins mGM-CSF/βhCG ( GC ) and hVEGF121/βhCG ( VC ) on RM-1 prostatic cancer and B16 F10 melanoma in the C57 BL/6 J mouse model. Two recombinant stains containing pET-28 a-mGM-CSF-X10-βhCGCTP37 and pET-28 a-VEGF-M2-X10-βhCG-CTP37 were induced by lactose to express fusion proteins. The fusion proteins were separated and purified to prepare the anti-tumor protein vaccines ( VC protein vaccine and GC protein vaccine) , which were then mixed to prepare a combined protein vaccine named VGC protein vac-cine. The prostatic cancer and melanoma tumor-bearing mice C57 BL/6 J were immunized with described vac-cines, then the growth of each tumor was measured;splenocyte proliferation of immunized mice was detected;and the cytotoxic effects of the vaccine on tumor cells were tested. After that, the in vivo concentrations of IFN-γ and anti-hVEGF antibodies were investigated by ELISA. The difference between each experimental group and normal saline group ( NS) was statistically significant in both tumor-bearing mouse models ( P <0. 05) respectively. Besides, VGC group exhibited significantly better anti-tumor effect compared with the GC and VC groups with the anti-tumor rate ( 41. 7 ± 0. 83)% and ( 46. 4 ± 1. 27)% for prostatic cancer and melanoma tumor, respectively. The co-administration of the two proteins, VC and GC, could inhibit the growth of RM-1 prostatic tumor and B16F10 melanoma effectively via anti-tumor immunity and anti-tumor angiogenesis.

Objective:To explore the risk factors of overt hepatic encephalopathy (OHE) in patients with liver cirrhosis.Methods:A retrospective study was designed. Patients with liver cirrhosis combined with /without OHE who were hospitalized to our hospital during the same period were selected as the case/control group. Clinical and laboratory data of both groups of patients were compared to analyze the risk factors affecting the occurrence of OHE. SPSS software was used for statistical analysis. A t-test or rank-sum test was used to compare the measurement data. Chi-square test or Fisher’s exact probability method was used to compare the count data. Logistic regression was used for multivariate analysis.Results:A total of 500 patients with liver cirrhosis diagnosed in our hospital from August 2017 to December 2018 were selected as the case group, and 40 cases with cirrhosis without OHE who were hospitalized during the same period were randomly selected as the control group. The gender composition and age of the case and control group were comparable. Viral hepatitis (mainly hepatitis B) was the main etiology of liver cirrhosis in both groups. There were 52.5% patients in the case group and 57.5% patients in the control group, respectively. Alcoholic liver disease, autoimmune liver disease and so on were the other included causes. With regard to blood biochemical indicators, the serum creatinine levels of both groups were comparable, but in the case group, the serum total bilirubin level was higher (34.30 μmol / L vs. 18.65 μ mol/L, Z = -3.185, P < 0.05), while the serum sodium level was lower (137.00 mmol/L vs. 140.08 mmol/L, Z = -2.348, P < 0.05), and the prothrombin time was longer (14.60 s vs. 12.20 s) s. 078, P < 0.05), and international normalized ratio (1.33 vs. 1.07, Z = - 5.632, P < 0.05), and serum albumin level (30.6 g/L vs. 35.6 g/L, t = 3.386, P < 0.05) was lower. In terms of complications, patients in the case group had a higher proportion of combined gastrointestinal bleeding (30.0% vs. 10.0%, χ 2 = 5.000, P < 0.05), ascites (87.5% vs. 30.0%, χ 2 = 27.286, P < 0.05) and secondary infection (32.5% vs. 10.0%, χ 2 = 7.813, P < 0.05). In terms of severity classification, the proportion of Child-Pugh C in the case group was higher (62.5% vs. 10.0%, χ 2 =26.593, P < 0.05). In terms of outcome, there were 3 deaths in the case group and no deaths in the control group. Multivariate analysis showed that Child-Pugh class C ( OR = 12.696), and combined ascites ( OR = 10.655) were an independent risk factor for OHE in patients with liver cirrhosis. Conclusion:Our single-center retrospective clinical study shows that patients with cirrhosis combined with OHE are more critical and have more complications. In order to promptly diagnose and treat OHE, more attention should be paid to patients with combined ascites and Child-Pugh class C.

Objective:To understand the clinical characteristics of hospitalized patients with liver cirrhosis, so as to provide theoretical basis for disease diagnosis and treatment, formulation of intervention measures, and improve the level of disease diagnosis and treatment.Methods:Hospitalized patients who were initially diagnosed with liver cirrhosis at Peking University First Hospital from August 2017 to December 2018 were selected retrospectively as the research objects. Liver cirrhosis demographic data, etiology, severity classification, incidence of complications, diagnosis and prognosis were recorded. Statistical analysis was performed using SPSS software.Results:Among all liver cirrhosis cases, there were 291 males and 209 females, with a male-to-female ratio of 1.4:1 and an age of 59.5±12.9 years as at August 2017 to December 2018. HBV infection, alcoholic liver disease, and autoimmune liver diseases were the most common etiology of liver cirrhosis. HBV infection alone, HBV infection combined with other factors, alcoholic liver disease alone, alcoholic liver disease combined with other factors, autoimmune liver disease alone, and autoimmune liver disease combined with other factors were presented in 163 (32.6%), 57 (11.4%), 47 (9.4%), 63 (12.6%), 85 (17.0%), and 22 (4.4.0%) cases, respectively. Ascites (221 cases, 44.2%), followed by esophagogastric varices (214 cases, 42.8%), and other including hypersplenism (137 cases), liver cancer (126 cases), upper digestive system tract hemorrhage (66 cases), hepatic encephalopathy (40 cases), infection (37 cases), portal vein thrombosis (23 cases), hepatorenal syndrome (20 cases) were the most common complications. The most common site of infection was the abdominal cavity (20 cases), accounting for 54.1%; followed by respiratory tract infection (8 cases), accounting for 21.6% in patients with liver cirrhosis with concurrent infection. Among them, there were 32 cases of bacterial infection alone, one case of bacterial infection combined with fungal infection, one case of bacterial infection combined with viral infection, and three cases of unknown pathogens. There were 69 cases in Child Pugh grade C, and the average hospitalization times were 12.6 days in terms of prognosis. There were total seven cases of death, of which five cases were due to upper gastrointestinal hemorrhage and two due to hepatic encephalopathy.Conclusion:HBV infection, ascites, and upper gastrointestinal bleeding were the most common etiologies, complications, and causes of death in patients with liver cirrhosis at our hospital.

Objective:To investigate the effectiveness of case-based learning (CBL) combined with online teaching in standardized residency training of rheumatology and immunology.Methods:A total of 78 individuals who participated in standardized residency training in Department of Rheumatology and Immunology in our hospital from June 2019 to August 2020 were included and divided into observation group and control group. The individuals in the control group received traditional teaching, and those in the observation group received CBL combined with online teaching. The physicians receiving standardized residency training were evaluated by theoretical examination, clinical operation skill assessment, and instructor rating, and the degree of satisfaction with teaching, degree of satisfaction with teaching methods, and classroom learning atmosphere were also evaluated.Results:The observation group had a theoretical examination score of (94.10±2.01) and a clinical operation skill assessment score of (90.44±1.57), which were significantly higher than those of the control group ( P<0.05), and the observation group had a significantly better instructor rating (89.36±1.33) than the control group ( P<0.05). Compared with the control group, the observation group had significantly higher degree of satisfaction with teaching (3.79±0.41), degree of satisfaction with teaching methods (3.92±0.27), and evaluation of classroom learning atmosphere (3.90±0.31) ( P<0.05). Conclusion:CBL combined with online teaching can help to improve learning efficiency, stimulate the enthusiasm for learning, expand clinical thinking, promote the growth of teaching and learning, and form a virtuous cycle among trainees receiving standardized residency training, which holds promise for further exploration.

Objective To investigate the interventional effect and mechanism of a novel ampe-lopsis hydrogel on dampness-heat accumulation syndrome of gouty arthritis.Methods A total of 90 patients with gouty arthritis who met the diagnostic criteria of western medicine and were differentiated as damp-heat accumulation syndrome of traditional Chinese medicine(TCM)were randomly divided into treatment group,control group and blank group,with 30 patients in each group.The blank group was treated with etoricoxib only,the control group was treated with etoricoxib combined with ampelop-sis hydrogel,and the treatment group was treated with etoricoxib combined with external application of ampelopsis hydrogel.The clinical efficacy,time to symptom improvement,safety,comfort,changes in syndrome scores of TCM,serum inflammatory factors[C-reactive protein(CRP),tumor necrosis factor-α(TNF-α),erythrocyte sedimentation rate(ESR)],NF-κB signaling pathway-related pro-teins,Visual Analogue Scale(VAS)scores for pain,and joint mobility were compared among the three groups before and after treatment.Results The total effective rates in the treatment group and control group were 93.33％and 90.00％,respectively,which were higher than 70.00％in the blank group(P＜0.05).The time for improvement of pain,redness,tenderness,and limited joint mobility in the treatment group was shorter than that in the control group and blank group(P＜0.05).After 7 days of treatment,the TCM syndrome score in the treatment group was lower than that in the con-trol group and blank group,and the levels of serum CRP,TNF-α,and ESR and the expressions of NF-κB signaling pathway-related proteins P50 and P65 in the treatment group and control group were lower than those in the blank group(P＜0.05).After 7 days of treatment,the VAS score in the treatment group was lower than that in the control group and blank group,and the comfort score in the treatment group was higher than that in the control group(P＜0.05).There was no significant differ-ence in the incidence of adverse reactions among the three groups(P＞0.05).Conclusion The effect of ampelopsis hydrogel in treating gouty arthritis is better than that of ampelopsis paste,and its mechanism may be related to the regulation of the NF-κB pathway and inhibition of inflammatory fac-tor expression.The hydrogel is easy to use,hygienic,and comfortable,and is expected to become a safe,effective,and convenient external medicine for gouty arthritis.

Objective To investigate the interventional effect and mechanism of a novel ampe-lopsis hydrogel on dampness-heat accumulation syndrome of gouty arthritis.Methods A total of 90 patients with gouty arthritis who met the diagnostic criteria of western medicine and were differentiated as damp-heat accumulation syndrome of traditional Chinese medicine(TCM)were randomly divided into treatment group,control group and blank group,with 30 patients in each group.The blank group was treated with etoricoxib only,the control group was treated with etoricoxib combined with ampelop-sis hydrogel,and the treatment group was treated with etoricoxib combined with external application of ampelopsis hydrogel.The clinical efficacy,time to symptom improvement,safety,comfort,changes in syndrome scores of TCM,serum inflammatory factors[C-reactive protein(CRP),tumor necrosis factor-α(TNF-α),erythrocyte sedimentation rate(ESR)],NF-κB signaling pathway-related pro-teins,Visual Analogue Scale(VAS)scores for pain,and joint mobility were compared among the three groups before and after treatment.Results The total effective rates in the treatment group and control group were 93.33％and 90.00％,respectively,which were higher than 70.00％in the blank group(P＜0.05).The time for improvement of pain,redness,tenderness,and limited joint mobility in the treatment group was shorter than that in the control group and blank group(P＜0.05).After 7 days of treatment,the TCM syndrome score in the treatment group was lower than that in the con-trol group and blank group,and the levels of serum CRP,TNF-α,and ESR and the expressions of NF-κB signaling pathway-related proteins P50 and P65 in the treatment group and control group were lower than those in the blank group(P＜0.05).After 7 days of treatment,the VAS score in the treatment group was lower than that in the control group and blank group,and the comfort score in the treatment group was higher than that in the control group(P＜0.05).There was no significant differ-ence in the incidence of adverse reactions among the three groups(P＞0.05).Conclusion The effect of ampelopsis hydrogel in treating gouty arthritis is better than that of ampelopsis paste,and its mechanism may be related to the regulation of the NF-κB pathway and inhibition of inflammatory fac-tor expression.The hydrogel is easy to use,hygienic,and comfortable,and is expected to become a safe,effective,and convenient external medicine for gouty arthritis.