Objective To investigate the immunogenicity of immunodominant cytotoxic T lymphocyte epitope E749-57 of human papilloma virus (HPV) 16 oncoprotein E7 chaperoned by heat shock protein (HSP)110. Methods Mouse HSP110 gene was cloned into prokaryotic expression vector pQE-80L for the expression of HSP110 protein, which was purified using Ni-NTA column. SDS-PAGE and Western-blot were conducted to confirm the purified mHSP110 protein, which was subsequently incubated with E749-57 peptide under heat shock condition, and high-performance liquid chromatography (HPLC) was used to evaluate the binding efficiency of the recombinant protein and E749-57 peptide. Twenty mice were divided into 4 groups to be immunized with mHSP110 protein, E749-57 peptide, mHSP110-E749-57 complex and phosphate buffered saline (PBS),respectively. Two weeks after the last immunization, spleen cells were collected from the immunized mice and divided into 2 parts: one were stimulated by E749-57 peptide followed by the detection of CD8+ INF-γ+ T cells with flow cytometry; the other one were subjected to MTT analysis for the estimation of cell proliferation. The mHSP110-E749-57 complex was also used to immunize TC-1 tumor bearing mice to observe its anti-tumor effect.Results The full-length 2577 bp-sized mHSP110 gene was amplified from mouse liver cDNA and cloned into pQE-80L vector. Direct sequencing confirmed the correctness of the cloning. SDS-PAGE and Western-blot demonstrated the successful purification of mHSP110. HPLC assay showed that the purified mHSP110 protein could bind with E749-57 to form a relatively stable protein complex. The percentage of IFN-γ+ CD8+ T cells in and proliferation index of spleen cells from the complex-immunized mice were statistically higher than those from the other 3 groups of mice. Moreover, the complex could obviously inhibit the growth of TC-1 tumor in mice. Conclusion The mHSP110-E749-57 complex could enhance the generation of specific cytotoxic T lymphocytes and exert anti-tumor effects in mice.

Objective To observe the application effect of therapeutic communication combined with WeChat intervention in patients with laryngoscopic examination. Methods A total of 220 patients with laryngoscopic examination were selected, and were randomly divided into control group receiving routine interventions and observation group receiving therapeutic communication and WeChat intervention, with 110 cases in each group. The mood status, compliance, patient′s satisfaction degree, and adverse reactions were compared between two groups. Results Before intervention, there were no significant differences in depression-dysphoria, confusion-disorientation, fatigue-sluggishness, anger-hostility, energy-vitality and tension-anxiety between the two groups (P > 0.05); after the intervention, these scores in the observation group were significantly lower than those in the control group (P < 0.05). The observation group demonstrated significant higher compliance scores in re-examination, nursing and treatment compared to the control group (P < 0.05). Before intervention, there were no significant differences in scores of communication and health education, nursing techniques, nursing attitudes, and examination environment between the two groups (P > 0.05); after intervention, the observation group showed significant higher scores of items mentioned above than the control group (P < 0.05). The incidence of adverse reactions was 1.82% (2 cases) in the observation group, which was significantly lower than 8.18% (9 cases) in the control group (P < 0.05). Conclusion Application of therapeutic communication combined with WeChat intervention can improve mood states and compliance of patients with laryngoscopic examination, enhance patient′s satisfaction degree, and reduce adverse reactions.