Purposes　To observe the clinicopathologic and immunohistochemical features of primary lymphoma of bone(PLB). Methods　Eight cases of PLB were investigated by light microscopy and immunohistochemical staining. Results　The mean age of eight cases of PLB was 51 years. Radiographs of all cases showed the lytic lesions or osteoporosises; All the cases were diffuse non-Hodgkins lymphomas(NHL), including 4 large cell lymphomas (centroblastic, CB), 2 centrocytic(CC), one mixed cell (CB-CC ) and one lymphoplasmacytic (LP); Immunohistochemical features revealed 6 cases of B-cell, 2 T-cell. Conclusions　The peak incidence for PLB is in the 5th decade with the radiologic appearance of lytic lesions or osteoporosises. All tumors are the diffuse NHL, including mainly large cell lymphomas and immunophenotypic features of B-cell lymphomas. Immunohistochemistry is helpful in the differential diagnosis of PLB.

To study the histogenesis, biological behaviour, classification of pituitary adenoma and the relationship between the symptoms of hormonal hypersecretion and the hormone test in pituitary adenoma. MethodsOne hundred and eighty cases of pituitary adenoma were investigated by clinicopatholgic analysis, and GH, PRL, ACTH, TSH, FSH, LH were examined in 110 cases by ABC immunocytochemical methods. ResultsForty percent of the patients were found to have the identical results between the clinical symptoms and hormonal test. Female patients were more than male ones, and the difference was satistically significant. The results showed that PRL and GH adenoma were often found in the cases by immunocytochemical method,and mixed GH-PRL adenoma were often found in the cases with multihormono-adenoma. ConclusionThe classification is practical and easy to operate, which combines the immunohistochemical examination of pituipary adenoma with the morphology and function.

AIM:To observe the effect of arsenic trioxide (ATO) on bleomycin-induced pulmonary fibrosis in rats. METHODS:Pulmonary fibrosis was induced in Sprague-Dawley (SD) rats by intratracheal instillation of bleomycin (BLM). The rats in ATO treatment group,steroid treatment group and model group were intraperitoneally injected with ATO,dexamethasone or normal saline (NS),respectively,while the control rats received NS both intratracheally and intraperitoneally. The effects of ATO were evaluated by analyzing the median survival time,hydroxyproline level in the lung,semiquantitative grading of alveolitis and pulmonary fibrosis,and quantitative analysis of the collagen in lung tissue (Masson's trichrome staining). Apoptosis index (AI) of the lung was detected by using the terminal transferase dUTP-digoxygenin nick endlabeling (TUNEL) method. The results of immunohistochemical staining for some cytokines were quantitatively analyzed. RESULTS:ATO (1) prolonged the median survival time of rats with BLM-induced pulmonary fibrosis at some extent; (2) attenuated the alveolitis and pulmonary fibrosis,reduced hydroxyproline level and collagen deposition in the lung tissue; (3) increased the AI of lung tissue at a certain phase; and decreased the levels of transforming growth factor-?1(TGF-?1) and tissue inhibitor of metalloproteinase-1 (TIMP-1),increased the content of in-terferon-? (IFN-?),but did not influence the concentration of matrix metalloproteinase-9 (MMP-9) significantly. CONCLUSION:ATO might attenuate BLM-induced pulmonary fibrosis in rats via increasing the AI in the lung tissue.

Objective:To explore the application effect of patient mutual assistance model guided by the hierarchy of needs theory in patients with acute myocardial infarction.Methods:Using the convenient sampling method, 40 patients with acute myocardial infarction in the Fourth People's Hospital of Haikou from September 2018 to October 2019 were selected as the control group and 40 patients with acute myocardial infarction from November 2019 to January 2021 were selected as the intervention group. The control group implemented the conventional nursing model, and the intervention group implemented the patient mutual assistance model guided by the hierarchy of needs theory on this basis. Perceived Stress Scale (PSS) , SDS and Herth Hope Scale (HHI) were used to evaluate the effect of intervention.Results:After the intervention, the total scores of PSS of patients in the two groups were lower than those before the intervention, and the difference was statistically significant ( P<0.01) . And the total PSS score of the intervention group was lower than that of the control group, and the difference was statistically significant ( P<0.01) . After the intervention, the total SDS score of patients of the two groups was lower than that before the intervention, and the difference was statistically significant ( P<0.01) . And the total SDS score of the intervention group was lower than that of the control group, and the difference was statistically significant ( P<0.01) . After intervention, the scores of HHI dimensions and total scores in two groups were higher than before intervention, and the differences were statistically significant ( P<0.01) . The scores of HHI dimensions and the total score of the intervention group were higher than those in the control group, and the differences were statistically significant ( P<0.01) . Conclusions:The patient mutual assistance model guided by the hierarchy of needs theory can effectively reduce the self-perceived pressure of patients with acute myocardial infarction after PCI, improve their depression and increase the level of hope.

Objective To elucidate the bidirectional causal relationship between lipid profiles and colorectal cancer(CRC)by using the two-sample and two-step Mendelian randomization(MR)methods,and to explore the mediating role and proportion of immune cells as intermediary factors.Methods The pooled statistical data related to the study were screened,and 179 lipids and CRC were analyzed using two-sample and two-step MR with the inverse variance weighted method.Simultaneously,the causal effect was verified via Bayesian weighted MR.Two-step MR analysis was conducted to determine whether a mediated effect was exerted on immune cell traits.Sensitivity,heterogeneity,and pleiotropy analyses were performed to verify the reliability of the study results.Results The causal relationship between nine lipid traits and CRC was preliminarily identified,and no reverse causal effect was found(P>0.05).The validity of the results was verified via Bayesian weighted MR(P<0.05).Twenty-seven types of immune cells were suggested to exert a causal effect on CRC.The causal effect of phosphatidylcholine(O-18:2_20:4)on CRC was determined via mediation analysis(OR:0.8579,95% CI=0.7395-0.9952,P=0.0429).The CD127-mediated proportion on CD45RA+CD4+T cells was 9.14% (β=-0.1052,P=0.0155).Conclusion A causal relationship exists between lipid traits and CRC,and the intervention of CD127 on CD45RA+CD4+T cell helps phosph-atidylcholine reduce the risk of CRC.