OBJECTIVE:To prepare nifuratel vaginal effervescent tablets(NVET)and establish a method for its quality control.METHODS:The NVET was prepared using acid or alkali effervescent by principal ingredient-separated granulation.The content of the principal ingredient was determined by UV spectrophotometry.RESULTS:The preparation was white or off-white colored tablet and its property was up to the standard stated in Chinese Pharmacopeia(2005 Edition)in identification and test.The linear concentration range of nifuratel was 100～400 ?g?mL-1(r=0.999 6)with an average recovery rate of 101.1%(RSD=0.62%,n=6).CONCLUSION:The preparation process for NVET is simple,and its quality is stable and controllable.

Objective To study the synthesis of methyl bryoanthrathiophene-1-carboxylate with high efficiency. Methods Target compounds were synthesized from 1,8-dihydroxy-9,10-anthraquinone via 5 steps including protecting, nitration, methyl mercaptoacetate substitution, intramolecular Aldol reaction and deprotecting. Results Target molecule was synthesized in the total yield of 45.8%. Conclusion Our synthesis of methyl bryoanthrathiophene-1-carboxylate is characterized by short synthetic route, high selectivity and high efficiency.

Objective To design and synthesize a new potential angiogenesis inhibitor, demethyl bryoanthrathiophene. Methods Target molecule-demethyl bryoanthrathiophene was synthesized in 5 steps using 1,8-dihydroxy-9,10-anthraquinone as the starting material, 5,7-dihydroxy-1-carboxyl-6-oxo-6H-anthra thiophene as the key intermediate and decarboxylization as the key step. Results The target molecule was synthesized with a total yield of 10.1% and identified by MS, IR, UV, 1H-NMR, 13C-NMR and 2D-NMR. Conclusion Our synthesis of demethyl bryoanthrathiophene is based on an easily-acquired starting material and accomplished with a simple synthetic route.

The basic course of medical specialty is an important part of medical education. There are few studies on effective teaching of basic courses of medical specialty. Based on the course of Pharmacology, this paper discusses the connotation, influencing factors and strategies of effective teaching of basic courses of medical specialty. The factors influencing the effective teaching of basic courses of medical specialty are multiple, that mainly includes four dimensions: students, teachers, course resources and teaching environment. Strategies to improve teaching effect include strengthening the idea of education, positioning goals hierarchically, optimizing curriculum structure, and innovating education platform.

OBJECTIVE:To evaluate the effects of arginine-enriched total parenteral nutrition(TPN)on the nutritional and immune parameters of patients undergoing abdominal surgery.METHODS:67 patients undergoing abdominal surgery were randomly assigned to receive routine TPN(control group,n=24),TPN plus 15 g arginine(n=25,trial group Ⅰ)or TPN plus 30 g arginine(n=18,trial group Ⅱ)for 3 days postoperatively.RESULTS:Arginene-enriched TPN significantly improved postoperative nitrogen balance,promoted the recovery of plasma-albumin and total protein,and elevated the levels of CD4+ and CD4+/CD8+,but no significant difference was noted for immunoglobulin,complement C3 and complement C4 during trial period.CONCLUSION:Arginene-enriched TPN can improve patients' postoperative nutritional and immune parameters and enhance the efficacy of TPN support.

Objective:To explore the speech features of female patients with anhedonic depression and their recognition of pleasure deficient symptoms.Methods:A total of 102 female depression patients who were hospitalized at Nanjing Brain Hospital from September 2020 to October 2021 were selected, including 62 anhedonic depression patients (anhedonic group) and 40 non-anhedonic depression patients (non-anhedonic group). A total of 50 female healthy controls were recruited during the same period.All participants were evaluated by the 17-item Hamilton depression scale (HAMD-17), Snaith-Hamilton pleasure scale (SHAPS), and the temporal experience of pleasure scale (TEPS), as well as voice acquisition.SPSS 23.0 software was used for data processing.Statistical analysis was conducted using one-way ANOVA, non-parametric tests, Logistic regression, and receiver operating characteristic curve.Results:Compared with the non-anhedonic group, the anhedonic group showed significant changes in 15 voice features(all P<0.05), including Mel-frequency cepstral coefficients, formant frequencies, intensity, and energy features.Among these features, Mel-frequency cepstral coefficients exhibited the highest accuracy in identifying anhedonic depression, with sensitivity of 47.5%, specificity of 91.9%, area under curve (AUC) of 0.751, 95% CI=0.686-0.866.Formant frequencies could identify female anhedonic depression, with a sensitivity of 90.0%, a specificity of 40.3%, an AUC of 0.647, and 95% CI=0.605-0.824.Energy features could identify anhedonic deficient depression, with a sensitivity of 60.0%, a specificity of 74.2%, an AUC of 0.679, and 95% CI=0.587-0.804.Intensity features could identify female anhedonic depression, with a sensitivity of 70.0%, a specificity of 58.1%, an AUC of 0.640, and 95% CI=0.554-0.769. Conclusion:Mel-frequency cepstral coefficients, formant frequencies, intensity features, and energy features may have specific changes in female patients with anhedonic depression.The Mel-frequency cepstral coefficients has the highest recognition accuracy for anhedonic symptoms in female depression patients, and is expected to become an objective evaluation index for female anhedonic depression.

Objective: To investigate the clinical and immunological laboratory features, mutations in SH2D1A gene and SAP protein expression in four children of two families with X-linked lymphoproliferative disease type 1(XLP-1). Method: Four patients (Family A including Patient 1 and Patient 2, Family B including Patient 3 and Patient 4) and their maternal relatives were enrolled in this study. The clinical manifestation, EBV infection status and chest CT scan were analyzed. The absolute and relative numbers of lymphocyte subsets, T lymphocyte proliferative response, SAP protein expression were assessed by flow cytometry. Quantification of signal joint TCR rearrangementexcision circle (sjTRECs), CDR3 spectratyping of TCRvβ and gene mutation of SH2D1A were detected by PCR based on genomic DNA or cDNA. Result: Four male patients from two families were diagnosed with XLP-1. The ages of disease onset were more than 1 year, more than 1 year, more than 1 month and 6 months. The ages at diagnosis were nine years and ten months, sixteen years and eight months, fourteen years and ten months, four years and nine months. All patients had recurrent infections and EBV infection. Patients 1, 2, and 3 had agammaglobulinemia and Patient 4 had hypogammaglobulinemia. Chest CT scan showed all patients had atelectasis and pneumonia, and Patient 3 had bronchiectasis. Patient 3 was diagnosised as Burkitt lymphoma. For immunological function, all patients exhibited reduced CD4/CD8 ratios, increased numbers of exhausted T lymphocyte, decreased number of NK cell. The numbers of total B lymphocyte and naïve B lymphocyte were normal, but the number of memory B lymphocyte declined in all cases. Four patients′ copy numbers of sjTRECs were low and CDR3 spectratypings of TCRvβ showed mildly skewed. But their T lymphocyte proliferative response was normal. SAP protein expression in four cases were measured by flow cytometry. Two patients from Family A were absent and two patients from Family B showed decreased values. SH2D1A gene sequence analysis showed that the patients of Family A harbored a nonsense mutation (c.163 C>T; p.R55X) in exon 2. Their mother and two sisters were carriers. A missense mutation of SH2D1A gene (c.278 G>A; p.G93D) in exon 3 was found in the patients of Family B. The mother was carrier. Four patients remain survived, Patient 3 gave up treatment, other three patients received IVIG therapy. Conclusion Four patients with XLP-1 from two families characterized by agammaglobulinemia have an extreme vulnerability to Epstein-Barr virus (EBV) infection. The functions of T cell, B cell and NK cell are impaired at different stages. The detection of SAP protein and SH2D1A gene are the key methods for diagnosis of XLP-1.