Objective: To illuminate the gene characteristics and clinical characterization of Coxsackievirus B5 (CV-B5) strains isolated from patients with sevre hand, foot and mouth disease (HFMD) in Qingdao city. Methods: A total of 1 844 patients of HFMD were consecutively admitted to Qingdao Women and Children's Hospital from 2013 to 2014. Information of the study population described above was collected retrospectively. The samples were collected from at least 1 site (throat swab, cerebrospinal fluid), which viral nucleic acid extracted and the entire VP1 gene sequences of CV-B5 isolates were amplified and sequenced, then the homology and phylogeny analysis were conducted by MEGA7.0. The prototype Faulkner strain and other VP1 amino acid sequences were derived from the GenBank database. Results: A total of 8 CV-B5 positive cases were obtained, including 4 males and 4 females; 6 severe hospitalized cases and 2 outpatients. The age of 6 hospitalized patients ranged from 3 to 48 months, with a median of 26 months. For the six inpatients, fever, convulsions vomiting, diarrhea and rash were the main clinical manifestation, and all combined with viral encephalitis. Compared with the prototype strain Faulkner, in the VP1 region,the nucleotide and the amino acid homologies was 77.3%-78.8% and 95.5%-97.0% respectively. Five out of the six severe cases with substitution of serine (S) to asparagine (N) at amino acid site 95 in the VP1 region. The sequences of 8 CV-B5 strains were classified into genogroup D. Conclusion Hand, foot and mouth disease associated with CV-B5 virus infection can result in nervous system involvement and the main complication was viral encephalitis. The CV-B5 strains associated with severe hand, foot and mouth disease had high nucleotide homology and present a certain regional aggregation.

Objective To investigate the relationship between active lifestyle and metabolic syndrome (MS) in the population of Shanghai Free Trade Zone companies and explore the effect of active lifestyle on controlling MS. Methods Collecting and analyzing the data of 526 employees in Shanghai Free Trade Zone companies by means of stratified-cluster sampling, who taken health examination from January 2006 to December 2007. All subjects were divided into active lifestyle A group (ALAG), active lifestyle B group (ALBG) and sedentary lifestyle group (SLG) based on their lifestyle surveys and physical activity questionnaires (PAQ). A variety of clinical in- dex and the prevalence of metabolic syndrome components were compared among the groups with different active lifestyles, as well as the relative risk of active lifestyle on MS. Results Body mass index (BMI), Waist-hip-ratio (WHR), blood pressure (SBP/DBP), fasting plasma glucose (FPG) and blood lipids (TG, TC, HDL-C) in the two active lifestyle groups (22.83?2.29) kg/m2, 0.862?0.037, (119.78?12.78)/(78.03?9.91) mm Hg, (4.82?0.78) mmol/L, (1.11?0.60) mmol/L, (4.44?0.78) mmol/L, (1.41?0.41) mmol/L and (24.43?2.32) kg /m2, 0.899?0.039, (127.16?15.64) /(81.63?9.12) mmHg, (1.51?0.84) mmol/L, (4.70?0.88) mmol/L, (1.35?0.47) mmol/L, (4.96?0.75) mmol/L) were significantly lower than those in SLG (all P0.05). Active lifestyle was a protective factor for MS with OR values as 0.154 (95%CI: 0.080～0.298) in ALAG; 0.609 (95%CI: 0.367～0.990) in ALBG, respectively. Conclusion Active lifestyle was a significant protective factor for MS. Health education on the population in Shanghai Free Trade Zone should be strengthening to help people establish scientific active lifestyle for preventing and controlling MS.

Objective To analyse the effect of mobilization and collection's time of peripheral blood stem cells(PBSC) from 8 cases of healthy donors. Methods The 10 donors were studied by self-control design.The number of aphereses was two times every donors. Healthy donors received rhG-CSF according to two different PBSC collection starting time: group 1:PBSC collection was starts 2 hours(2 h) after the fourth day or the fifth day of rhG-CSF. group 2:PBSC collection was starts 4 hours(4 h) after the fourth day or the fifth day of rhG-CSF.(The first dose of rhG-CSF was given on day 1, considering day 0 as the day before starting mobilization). In this study we have compared with two groups of apheresis product. Results The MNC count was significantly higher for donors 4 h collection (groups 2) then 2 h. ( groups 1)(P