ObjectiveTo investigate the effect of β-adrenoceptor (β-AR) blockers on G protein and heart function changes in rats with acute myocardial infarction (AMI) MethodsWistar rats with AMI induced by left anterior descending coronary branch ligation were randomly divided into compared with sham operation group. Eight weeks after therapy, hemodynamics was assessed by inserting catheters and the level of G protein was detected by Western blot analysis. ResultsCompared with the sham operation group, systolic blood pressure(SBP), diastolic blood pressure (DBP), left ventricular systolic pressure(LVSP) and left ventrieular pressure maximal rate of rise and fall(±dp/dtmax) in AMI group were significantly decreased, while left ventricular end diastolic pressure (LVEDP) and Gs and Gi protein levels were significantly increased (all P<0.05). Compared with AMI group, LVSP and ± dp/dtmax were increased, but LVEDP and Gi protein level were significantly decreased in metoprolol and carvedilol group. LVEDP and Gi protein level were decreased in carvedilol group compared with metoprolol group. ConclusionsCarvedilol can effectively suppress the change of G protein and improve the heart function after AMI, and the effect is better than that of metoprolol. This may be related with its β2-AR blocking effect.

Objective:To investigate the prevalence,and the factors that influence depressive symptoms among chronic respiratory disease patients in tertiary hospital.Methods:A total of 1713 outpatients and inpatients with chronic respiratory disease were selected from 8 tertiary hospitals in Jiangsu Province from July to September,2014 and screened according to the Hospital Anxiety Depression Scale-D (HADS-D).A questionnaire developed by this research group,was used to collect demographic and clinical information.Logistic regression was used to identify factors that were associated with depressive symptoms.Results:The overall rate of depressive symptoms was 46.0％.Multiple logistic analysis showed that spinsterhood (OR ＝ 0.45),higher education level (middle school /high school/technical school OR ＝0.65;college degree or aboveOR ＝0.28),BMI ≥24 (OR ＝0.71) were associated with decreased risk of depressive symptoms (P ＜ 0.05).B MI ＜ 18.5 (OR ＝ 1.52,),average income of family ≥10000 RMB (OR ＝ 1.37-1.96),limited daily activities (OR ＝ 1.72),poorer sleep quality (OR ＝ 1.45),and negative life events (OR ＝ 1.62) were associated with increased risk of depressive symptoms (P ＜ 0.05).Conclusion:The prevalence of depressive symptoms among chronic respiratory disease patients in tertiary hospitals in Jiangsu Province was higher.Marital status,education level,income,BMI,limited daily activities,subjective sleep quality,negative life events may be the related factors of depressive symptoms of chronic respiratory diseases patients.

OBJECTIVETo explore the influence of recombinant human growth hormone (rhGH) on the apoptosis and intestinal mucosal structure in severely scalded rats.

METHODSThirty male Wistar rats were randomly divided into three groups, i.e. control, scalding and rhGH groups. The rats in scalding and rhGH groups were inflicted with 25% TBSA III degree scalding on the back and immediately followed by intraperitoneal injection of dexamethasone (80 mg/kg). The scalded rats were administered with normal saline and rhGH (1.33 IU.kg(-1).d(-1)) since 2 postburn hours (PBHs), respectively in the last two groups. The changes of the apoptosis rate, the intestinal mucosal proliferative index (PI) and epithelial ultrastructure and the intestinal mucosal pathomorphology of the distal end of ileal mucosal tissue were observed on 30 and 96 PBHs.

RESULTSThe intestinal mucosa morphology and epithelia in scalding group were severely injured but were significantly ameliorated by rhGH to near those in control group. The PI in rhGH and scalding groups at 30 PBHs was evidently higher that that in control group (P < 0.05 - 0.01). But the PI exhibited no obvious difference between scalding and rhGH groups. While the PI in rhGH group at 96 PBHs was obviously higher than that in both scalding and control groups (P < 0.01). The intestinal mucosal epithelial apoptotic rate in scalding group was significantly higher than that in control group (P < 0.01), while that in rhGH group was evidently lower than that in scalding and control groups (P < 0.05 - 0.01).

CONCLUSIONrhGH could promote postburn intestinal mucosa epithelial proliferation in slow - action manner and inhibit intestinal mucosal epithelial apoptosis with rapid and obvious effects. As a result, the intestinal mucosal epithelial injury could be ameliorated by rhGH by means of its inhibiting roles and the normal morphological structure of intestinal mucosa was maintained ad hoc.

Animals ; Apoptosis ; Burns ; pathology ; Human Growth Hormone ; genetics ; pharmacology ; Intestinal Mucosa ; drug effects ; pathology ; Male ; Rats ; Rats, Wistar ; Recombinant Proteins ; pharmacology