Objective:To prepare specific IgY production using Actinobacillus actinomycetemcomitans(A.a) immunizing hen, and then to investigate anti-Actinobacillus actinomycetemcomitans IgY inhibiting growth of A.a and Capnocytophaga gingivalis(C.g). Methods:Using immunization method, water-dilution method, two-step ammonium sulfate precipitation, inhibiting bacteria growth test in liquid anaerobic culture, and ELISA, IgY were induced, extracted, purified, and inhibiting growth of A.a and C.g by the IgY was roundly evaluated. Results:The IgY purity reached to 85.6%~90.3% through 550 g/L and 330 g/L ammonium sulfate precipitation, and efficacy value was 1∶32 000. The IgY efficacy value of anti- A.a was 1∶8 000 against C.g in cross-reactivity.When IgY concentrations of anti-A.a were in the 5.0,1.0,0.1 g/L and concentration of A.a was in the 5?108 CFU/L, the suppression rate of A.a growth were 31.60%(P=0.004),10.24%(P=0.024),-3.30% respectively during 24 h culture and were 64.20%(P=0.004),53.21%(P=0.002),11.20% respectively in 72 h culture. When the concentration of A.a was in the 1?108 CFU/L, the suppression rate of A.a growth were 35.71%(P=0.004),30.95% (P=0.012),11.11% respectively during 24 h culture, and were 65.11%(P=0.005),54.04%(P=0.002),16.17% respectively during 72 h culture. When 5.0 g/L IgY of anti-A.a was cultured with 1?108 CFU/L C.g for 24 h, the suppression rate of C.g growth was 41.61%(P=0.005), and for 72 h it was 86.99%(P=0.014). Conclusion:The hen is able to be induced to produce high efficacy value IgY of anti-A.a by A.a. The specific IgY of anti-A.a is capable of inhibiting A.a and C.g growth. There are common antigens and cross immunizing reactivity between A.a and C.g.

Objective:To observe the resistance of hypericum japonicum Thunb.to helicobacter pylori.Methods:Liquid dilution method was used to culture mixture of hypericum japonicum Thunb.extractum and helicobacter pylori,hypericum japonicum Thunb.extraction and helicobacter pylori respectively.The minimum inhibitory concentration and minimal bactericidal concentration of hypericum japonicum Thunb.extractum and extraction were determined by comparing the growth condition of helicobacter pylori.Results:Both of hypericum japonicum Thunb.extractum and extraction had obvious resistant effect on helicobacter pylori.Conclusion:25 mg/ml hypericum japonicum Thunb.extractum and 6.25 mg crude drug/ml hypericum japonicum Thunb.extraction can suppress the growth of helicobacter pylori effectively.

0.05).Combination of NGF with bFGFs(10 U/ml NGF+10 ?g/L bFGF or 5 U/ml NGF+5 ?g/L bFGF) not only promoted the proliferation of HDPCs(P

ObjectiveTo study the effect of extract of fetal brain (Nerval Active Factors, NAF) on learning, memory and stamina of mice.Methods51 mice were divided into five groups randomly: normal saline as Group A, Cerebrolysin as Group B, NAF as Group C:0.1mg,Group D:0.2mg,Group E:0.4mg, i.p.The time looking for food and error frequency of walking road were tested by using complicated maze, and the drowning time was tested when the mice carried 5% body weight on its back.ResultsBefore and after treatment,the time looking for food in maze showed no significant change in Group A(P>0.1) and was significantly shorter in Group B, C, D and E(P<0.005,P<0.05,P<0.001,P<0.001).There was no significant difference on error frequency of walking road in Group A and B(P>0.2,P>0.5) before and after treatment, while it was significantly decreased in mice of Group C, D and E(P<0.02,P<0.02,P<0.01).There was significant difference on drowned time of mice carrying 5% body weight on back in five groups by analysis of variance (P<0.05) after treatment, but this difference derived from C vs E(P<0.05)and D vs E(P<0.01).Conclusions NAF can raise the activity of learning and memory of mice, as well as increase their stamina. On the other hand, the stamina of mice decreases when NAF is given more than 20 mg/kg body weight.