1.The effect of dendritic cells on allergic rhinitis in sublingual therapy.
Shuangxi LIU ; Rongming GE ; Shaoqing YU
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(14):1089-1092
Dendritic cells (DCs) is known as the most potential and professional antigen presenting cells (APC), it mainly involves in the cellular immunity and T cell dependent humoral immunity, which plays a key role in the immune response and is one of the most hot areas in immunology in recent years. DCs plays a key role in allergic rhinitis (AR) and is one of the most important mechanism of AR treating by sublingual immunotherapy (SLIT). This article reviewed the mechanism of the role of DCs in AR and AR treating by SLIT.
Animals
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Dendritic Cells
;
immunology
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Desensitization, Immunologic
;
Humans
;
Rhinitis, Allergic
;
therapy
;
Sublingual Immunotherapy
2.A case report of vagus nerve invasion with disseminated herpes zoster.
Shuangxi LIU ; Rongming GE ; Na CHE
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2013;27(19):1094-1095
A case of vagus nerve invasion with disseminated herpes zoster was reported. Clinical manifestation of disseminated herpes zoster and vagus nerve injury. relevant imaging examination and laboratory examination can help to establish a preliminary diagnosis. Anti-virus, anti-infection and symptomatic treatment had been performed and showed good clinical efficacy.
Aged
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Herpes Zoster
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pathology
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Humans
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Male
;
Vagus Nerve
;
pathology
3.Clinical analysis of simple orbital blowout fracture.
Wen XU ; Chuanliang ZHAO ; Ling JIN ; Rongming GE
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(5):418-421
OBJECTIVE:
To discuss the clinical features and treatment methods for simple orbital blowout fracture.
METHOD:
Retrospective analysis of the CT images of 16 orbital blowout fracture case, and identification of the sites, degree, patterns and features of fractures. Among the 16 cases, 2 cases adopted conservative treatment; 11 cases gained a reduction of orbital fracture through endoscopic transnasal surgery; the other 3 patients choosed endoscopic transnasal surgery and Caldwell-Luc operations.
RESULT:
Among 16 diplopia cases, 13 cases were completely cured, and 3 patients' vision were significantly improved. Among 11 enophthalmos cases, 10 patients were cured, and the effect of the other one was not satisfied. Among the 15 eye movement disorder cases, 13 patients' eye movement gained a full recovery, and the other 2 cases were nearly normal. All patients' vision were improved in different extents, and no one got a complication.
CONCLUSION
Computerized Tomography is helpful to the diagnosis of simple orbital blowout fracture. Caldwell-Luc operation with transnasal endoscope is an effective method for the treatment of orbital fractures.
Diplopia
;
etiology
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Endoscopy
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Enophthalmos
;
etiology
;
Humans
;
Orbital Fractures
;
complications
;
surgery
;
Retrospective Studies
;
Tomography, X-Ray Computed
4.Twenty-six clinical case analysis of defect repairing after the resection of basal cell carcinoma on external nose.
Wen XU ; Yijun CAO ; Wei TANG ; Ling JIN ; Shaohui LI ; Rongming GE
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(19):1505-1507
OBJECTIVE:
To study a surgical and repairing method for defects after the resection of basal cell carcinoma of external nose.
METHOD:
There are 26 cases of basal cell carcinoma that tumors have been resected completely after operation. For defect repairing in those 26 cases, 2 cases adopt direct suture method; 2 cases use skin graft repairing methods; 18 cases employ naselabial skin flap repairing method; 4 cases choose forehead pedicle skin flap repairing methods.
RESULT:
the wound of all the 26 cases was primary healed. Additionally, skin flap and skin graft were all survived. Follow-up studied in patients 1 to 3 years after the surgery showed that the local scar was not obvious and no tumor recurred or transferred.
CONCLUSION
Different surgical and repairing methods are performed to obtain a satisfactory results based on the area of defect and its location in nose. Naselabial skin flap is especially an ideal method to repair defects.
Carcinoma, Basal Cell
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surgery
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Cicatrix
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Forehead
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Humans
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Neoplasm Recurrence, Local
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Nose
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Nose Neoplasms
;
surgery
;
Reconstructive Surgical Procedures
;
Skin
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Skin Neoplasms
;
surgery
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Skin Transplantation
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Surgical Flaps
;
Wound Healing
5.The expression and relationship of CXCR4 and microvessel density in laryngeal squamous cell carcinoma.
Chuanming ZHENG ; Rongming GE ; Minghua GE ; Xicai SUN
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2011;25(3):123-126
OBJECTIVE:
To investigate the expression of chemokine receptor 4 (CXCR4) in laryngeal squamous carcinoma and its correlation with clinicopathology character and the building of microvessel density (MVD). To evaluate its role in the carcinogenesis and progression in laryngeal squamous carcinoma.
METHOD:
The expression of CXCR4 in fresh laryngeal squamous carcinoma tissues and adjacent normal tissues from 42 patients were examined by RT-PCR, immunohistochemical staining and Image-pro-plus software. The numbers of regeneration blood vessels in the laryngeal squamous carcinoma was counted by antibody against factor V associated antigen and immunohistochemical analysis.
RESULT:
The positive expression rate of CXCR4 in tumor samples was significantly higher than that in normal ones (P < 0.01). In tumor samples, the expression of CXCR4 were not associated with age, sex, tumor site and T stage (P > 0.05), while it were higher in tumors of grade III, IV than in grade I, II of pathology classification (P < 0. 01). The expression of CXCR4 were significantly higher in tumors with cervical lymph node metastasis than that in tumor without cervical lymph node metastasis (P < 0.01). The expression of CXCR4 protein and CXCR4 mRNA were at the same level. The expression level of CXCR4 in the laryngeal squamous carcinoma tissue was positively correlated with vascularization.
CONCLUSION
The higher expression of CXCR4 may play a key role in the invasion and metastasis of laryngeal squamous carcinoma, and were correlated with micro-vascularization.
Adult
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Aged
;
Aged, 80 and over
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Carcinoma, Squamous Cell
;
blood supply
;
metabolism
;
pathology
;
Female
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Humans
;
Laryngeal Neoplasms
;
blood supply
;
metabolism
;
pathology
;
Lymphatic Metastasis
;
Microvessels
;
pathology
;
Middle Aged
;
Neoplasm Staging
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Neovascularization, Pathologic
;
pathology
;
Receptors, CXCR4
;
metabolism
6.Effect of lung protection mechanical ventilation on respiratory function in the elderly undergoing spinal fusion.
Yeying GE ; Liyong YUAN ; Xiaohong JIANG ; Xiuzhen WANG ; Rongming XU ; Weihu MA
Journal of Central South University(Medical Sciences) 2013;38(1):81-85
OBJECTIVE:
To determine the effect of lung protection mechanical ventilation on respiratory function in the elderly undergoing spinal fusion.
METHODS:
Sixty 70-85 year old patients, ASA class II or III, undergoing spinal fusion were randomly assigned into 2 groups (30 in each group): a protection mechanical ventilation group (group P) and a conventional mechanical ventilation group (group C). Low VT and low level positive end expiratory pressure (PEEP) mechanical ventilation were applied in group P (VT=6mL/kg, RR=12-18 b/min, I:E=1:2, PEEP=10 cmH2O, alveolar recruitment performed once every 15 min), while traditional ventilation was used in group C ( VT=10-12 mL/kg, RR=12 b/min, I:E=1:2). Arterial blood samples were taken and pH, PaO2, PaCO2, PaO2/FiO2, A-aDO2, HR, SBP, DBP and CVP were recorded before the operation (T0), 1 h after tracheal intubation (T1), tracheal extubation immediately (T2), 1 h after tracheal extubation (T3), 1 d, 2 d, and 3 d after the operation (T4, T5, and T6). The pulmonary complication was also examined 1 d after the operation.
RESULTS:
At T1, T2, T3, T4 and T5, PaO2 and PaO2/FiO2 in group P were higher than those in group C, but A-aDO2 in group P was lower than that in group C. Five patients had bronchitis, 5 had hyoxemia, and 3 had atelectasis in group C, but 2 bronchitis in group P. The incidence of pulmonary complication was 43.3% in group C and 6.6% in group P. There was no significant difference in HR, SBP, DBP and CVP between the 2 groups.
CONCLUSION
Lung protection mechanical ventilation improves the arterial oxygenation and accelerates the recovery of respiratory functions in elderly patients after spinal fusion operation, with no influence on hemodynamics.
Aged
;
Aged, 80 and over
;
Anesthesia, General
;
Female
;
Humans
;
Hypoxia
;
prevention & control
;
Male
;
Positive-Pressure Respiration
;
methods
;
Postoperative Complications
;
prevention & control
;
Pulmonary Ventilation
;
Respiration, Artificial
;
methods
;
Respiratory Function Tests
;
Spinal Fusion
;
methods
7.The configuration of soft palate muscular phenotype in obstructive sleep apnea hypopnea syndrome.
Siyi ZHANG ; Rongming NIE ; Pingjiang GE ; Shaofeng LIU ; Zhongming LU ; Runmei GE ; Xiaoli SHENG ; Shaohua CHEN
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2011;25(9):415-422
OBJECTIVE:
To study the mRNA expression of muscle phenotype and collagen of soft palate and pathology in obstructive sleep apnea hypopnea syndrome (OSAHS).
METHOD:
We used the Real-time PCR to test the mRNA expression of soft palate muscle myosin heavy chain (MyHC) phenotype and collagen in 12 OSAHS patients and 8 control patients. We also distinguished the muscle isoforms I , II with ATPase staining, then counted the numbers of isoforms muscle fiber.
RESULT:
The mRNA expression of OSAHS group was more than control group in II A MyHC phenotype (P<0.01). The number of OSAHS group muscle fibre I isoform was less than control group with pH4. 3 ATPase staining (P<0.05).
CONCLUSION
Compare to control group, the enhancement happened in the mRNA expression of II A MyHC phenotype which can increase the velocity and power but de crease the enduring quality of muscle in OSAHS, and the reduce be in the I MyHC isoform of muscle fiber that can cause muscle velocity become slower and persistency become longer in OSAHS patients.
Case-Control Studies
;
Female
;
Humans
;
Male
;
Middle Aged
;
Muscle Fibers, Skeletal
;
metabolism
;
pathology
;
Myosin Heavy Chains
;
metabolism
;
Palate, Soft
;
metabolism
;
pathology
;
Phenotype
;
Protein Isoforms
;
metabolism
;
RNA, Messenger
;
genetics
;
Sleep Apnea, Obstructive
;
metabolism
;
pathology
9. Experimental study on dendritic cells modified by dust mite allergen gene in the treatment of allergic rhinitis in mouse
Na CHE ; Bing HAN ; Shuangxi LIU ; Rongming GE ; Shaoqing YU
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2018;53(9):675-679
Objective:
To explore the therapeutic effects of dendritic cells (DC) modified by the dust-mite-allergen(
10. Effect of hydrogen-rich saline on the CD4+ CD25+ Foxp3+ Treg cells of allergic rhinitis guinea pigs model
Feifei XU ; Shaoqing YU ; Chuanliang ZHAO ; Na CHE ; Ling JIN ; Qiuli WANG ; Rongming GE
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2017;52(7):506-511
Objective:
To explore the effect of hydrogen-rich saline on the CD4+ CD25+ Foxp3+ Treg cells in a guinea pig model of allergic rhinitis (AR) and investigate the underling anti-inflammatory mechanism.
Methods:
Using random number table, eighteen guinea pigs were divided into three groups (control group/AR group/HRS group,