70%. The cluster analysis and factorial analysis showed that the fierceness, cruelty, and vital qi damaging were often clustered with each other and constituted the factors of main characteristics of Du-pathogen. In the Logistic regression analysis, it was discovered that fierceness, cruelty, and multi-damaging were closely related with the recognition of "the excessive pathogen damaging the health" (P

Objective To study the relations among phlegm turbidity, blood stasis syndromes, Interleukin-6 (IL-6) and highly sensitive C-reactive protein (hs-CRP) in simple hypertension patients and those complicated with abnormal blood fat and/or diabetes.Methods Totally 319 cases of hypertension collected were divided into the group with simple hypertension (simple group, 98 cases) and the group with hypertension complicated with abnormal blood fat and/or diabetes (complicated group, 221 cases). The patients were classified according to the 4 different syndromes, i.e., phlegm turbidity, blood stasis, phlegm turbidity plus blood stasis, and not phlegm turbidity not blood stasis. ELISA was used to measure serum IL-6 content, and latex enhancement immune turbidimetry was applied to determine hs-CRP content.Results The contents of IL-6 and hs-CRP of the two groups were not significantly different. The content of hs-CRP of both groups in the 4 syndromes was not significantly different. In the simple group, the content of IL-6 in the 4 syndromes was not significantly different either. In the complicated group, the content of IL-6 in the syndrome of phlegm turbidity plus blood stasis was significantly higher than in other syndromes (P

Objective To study the personality characteristics of the stagnation of liver-qi syndrome cases with Minnesota Multiphasic Personality Inventory (MMPI).Methods 20 liver-qi stagnation syndrome cases and 20 normal persons whose paired by sex and age were tested by MMPI. The scores of 13 clinical scales and the section plane of MMPI were acquired and analyzed. Results There was a significant difference in 9 clinical scales (F, Pa, D, Pt, Si, Sc, Hs, Hy, Pd) between the liver-qi stagnation syndrome cases and normal persons ( P<0.05～0.001). The scores of Hs, D, Hy, Pd in the liver-qi stagnation syndrome group were higher than the Chinese normal model. The MMPI section plane of liver-qi stagnation group presented the type of 3/1. Conclusion The patients with liver-qi stagnation have some special personality characteristics such as depression, anxiety, loneliness, indifferent and attention.

Objective To investigate the effects of anxiety and anti - anxiety therapy on vascular endothelium function and platelet activation in patients with acute coronary syndrome (ACS).Methods One hundred and thirty -nine ACS patients were enrolled in this prospective and controlled clinical study from January 2009 through December 2010.Patients with severe heart failure,liver and renal dysfunction,infection,allergy to benzodiazepines and taking antipsychotic drugs in recent 2 weeks as well as patients unable to complete the questionnaire were excluded.All these patients were divided into the anxiety group ( n =68 ) and the non - anxiety group ( n =71 ) according to Hamilton Anxiety Scale (HAMA).The plasma levels of NO,ET,CD62p,CD63 and flow- mediated diastolic functions (FMD) of humeral arteries were measured.The patients in anxiety group were randomly assigned to group A ( n =34 ) and group B ( n =34).Lorazepam in a dose of 0.5 mg twice a day and Vitamin B6 in dose of 10 mg twice a day as placebo were prescribed for patients of Group A and B respectively.After 2 weeks,all above variables of group A and group B were measured once again as well as the score of Hamilton Anxiety Scale.The chi - square test was used for constituent ratios,while t - test was applied to analysis of differences in above variables between two groups.Results The plasma level of NO and FMD of humeral artery in the anxiety group were significantly lower than those in the non -anxiety group (t =2.090 and 2.558,P =0.038 and 0.012,respectively),and the plasma levels of ET,CD62p and CD63 in the anxiety group were significantly higher than those in the non - anxiety group ( t =2.082,2.042 and 2.145,P =0.039,0.043 and 0.034,respectively).There were no statistical differences in all above variables as well as HAMA score between group A and group B before anti - anxiety treatment.Two weeks after treatment,the level of NO and FMD of humeral artery in group A were significantly higher than those in group B ( t =2.821 and 2.246,P =0,006 and 0.028,respectively) and the levels of ET,CD62p,CD63 and HAMA score in group A were significantly lower than those in group B ( t =2.107,3.242,2.079 and 7.779,P =0.039,0.002,0.041 and ＜0.01,respectively).Conclusions Anxiety mood markedly aggravates the disorder of vascular endothelial function and platelet activation,and both of them can be improved by anti - anxiety therapy.Consequently,the intervention in anxiety mood may improve the outcomes of ACS patients.

Objective To investigate the prevalence and severity degree of post traumatic stress disorder(PTSD), anxiety and depression, and to explore the different mental profile in different groups involved with an earthquake. Methods 26 days after 8.0 grade earthquake in Wenchuan, psychological rescue team of State Administration of Traditional Chinese Medicine went to some communities in Deyang and Mianzhu area of Sichuan province. In this period, a total of 119 survivors, including students(42), teacher(40) and masses(37), were investigated through impact of event scale (IES), self-rating anxiety scale (SAS) and self-rating depression scale(SDS). Results①The detection rate of score over 19 in IES in 3 groups (students, teacher and masses) were 69％, 80％ and 91.9％, respectively. There was significant difference among these 3 groups (F=5.611, P=0.005<0.01) in respect of severity degree of PTSD. ②The values of severity degree of anxiety in 3 groups were significant higher than normal value (P<0.001, all). There was also significant difference among these 3 groups (F=3.376, P=0.038<0.05) in respect of severity degree of anxiety, with the masses group being significant higher than student and teacher group (P=0.029, P=0.022, respectively). ③The values of severity degree of depression in these 3 groups were significant higher than normal value (P<0.05, all). But there was no significant difference (F= 0.670, P=0.514) among these 3 groups. ConclusionThe results suggested the importance to assess PTSD, anxiety and depression in post-disaster area, especially PTSD and anxiety evaluations in the masses group.

Objective: Adolescents are at a special stage of physical and mental development, which is a susceptible period for mental disorders. Since the outbreak of coronavirus pneumonia in December 2019, long term stress may have negative effects on the mental health of the adolescents. In the context of the coronavirus disease 2019 (COVID-19), the study was designed to investigate the mental and psychological health of adolescents in China and its possible related factors. Methods: A cross-sectional study design was adopted using a structured questionnaire which were distributed through the Internet to measure depression, anxiety, life events and stress related factors. Descriptive statistics and multiple regression analyses were conducted to process the data. Results: The final sample comprised 795 adolescents. The total detection rate of depression was 76.48% and the total detection rate of anxiety was 33.08%. ANOVA showed that there were significant differences in depression scores in terms of gender, anxiety scores, history of mental disorders, COVID-19 knowledge reserve, family and social contradictions (p<0.05). And there were significant differences in anxiety scores in terms of gender, depression scores, mental health knowledge reserves, family and social contradictions (p<0.05). Multiple regression analysis showed that anxiety score, health status and COVID-19 knowledge reserve were positively associated with depression score (p<0.01), and history of psychosocial disorders was negatively associated with depression score (p<0.05); depression score, family and social contradictions were significantly positively correlated with anxiety score (p<0.01), and history of mental disorders was significantly negatively correlated with SDS score (p<0.01). Conclusion During the outbreak of COVID-19, adolescent students with better understanding of the pandemic, more complete knowledge of mental health, and better family and social relationship had less impact on their mental health. Therefore, to ensure a sound social support system, elaborate health instruction, and family communication and mutual understanding are conducive to alleviating the psychological stress caused by the epidemic, and it is positive for adolescent students to maintain a good mental health.

Objective:To establish a diagnostic system for heat toxin syndrome of acute cere-bral infarction.Based on this toxin syndrome diagnostic system,the general principles of heat toxin development will be uncovered,and the critical turning point at which the heat toxin syndrome occurs will also be explored.Methods:In this study,a total of 271 hypertension patients with cerebral infarction within 72 h were recruited from the Affiliated Dongfang Hospital of the Beijing University of Chinese Med-icine,the Affiliated Dongzhimen Hospital of Beijing University of Chinese Medicine,the Affil-iated Renmin Hospital of Peking University,the Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,the Affiliated Hospital of Shandong University of Traditional Chinese Medicine,the Affiliated Hospital of Changchun University of Traditional Chinese Medicine,the Affiliated Hospital of Hebei University of Traditional Chinese Medicine and China Meitan General Hospital from August,2008,to December,2009.The patients' Chi-nese medical information was recorded on days 1,3,5,7,and 14 during their hospitalizations.The medical records were recorded according to traditional Chinese medicine (TCM) theory and included the serum marker levels at the beginning and at the end of the trial.The time line was also analyzed.Results:The level of Hs-CRP,PAG,NSE,OX-LDL,and MMP-9 were abnormal and,were higher in CI patients compared to hypertension patients.In the study of the heat toxin diagnosis system,according to the entropy clustering results,30 combinations of the medical information can be sorted into the traditional syndromes,but 13 combinations cannot be sorted.To obtain more precise symptoms related to the heat toxins,a logistic regression equation was set up with the variables from the unsorted medical information;the dependent variables were fever and BP fluctuation.Weighted variables were obtained.MLP analysis demonstrated that the diagnosis model was stable and precise.The accuracy reached 83.82％.The ROC test showed that seven points of the diagnosis system was the best cutting point,with a sensitivity of 0.857 and a spec-ificity of 0.955.Progressing stroke was related to heat toxin syndrome.When the turning point appeared,the combination of symptoms,such as coma,aphasia,gummy eyes,and halitosis,predicted the deterioration or recovery of Cl.The heat toxin syndrome existed in every sub-type of Cl;however,the observed heat toxin levels were highest in PACl and lowest in LACl.Meanwhile,blood and sputum stasis syndromes transformed into heat toxicity were one source of heat toxin syndrome.Conclusion:Heat toxin syndrome,as well as qi/blood/sputum stasis,co-existed in the CI pa-tients,and the transformation frequently appeared during the process.Three to five days after the onset of Cl was the turning point,at which time several combinations of medical indicators make it possible to predict the development of Cl.

Objective To study the therapeutic effect of Ningxin Anshen Fang on insomnia, and regulative mechanism to the metabolic loop of glutamic acid (Glu)/γ-aminobutyric acid (GABA)-glutamine (Gln).Methods The model of insomnia was established by using modified multiple water platform sleep deprivation method in rats, and then all rats were randomly divided into normal group, model group, diazepam group, Ningxin Anshen Fang low-dose group (low-dose group), Ningxin Anshen Fang mid-dose group (mid-dose group) and Ningxin Anshen Fang high-dose group (high-dose group).The general conditions were observed, sleep latency and sleep duration were observed by applying pentobarbital sodium (PS) cooperative sleep test, and model and curative effect of Ningxin Anshen Fang on insomnia were reviewed.The content of Glu and GABA in brainstem and hypothalamus was detected by using high performance liquid chromatography (HPLC).The expressions of glutamic acid decarboxylase (GAD) and astrocyte glutamine synthetase (GS) in brainstem and hypothalamus were detected by using Western blot experiment.Results The sleep latency was significantly shortened, sleep duration was prolonged, GABA level in brainstem and hypothalamus was increased and ratio of Glu to GABA (Glu/GABA) was decreased by Ningxin Anshen Fang in insomnia rats.The expression of GAD in brainstem and hypothalamus was significantly promoted in all Ningxin Anshen Fang groups.Conclusion Ningxin Anshen Fang can affect the synthesis and decomposition Glu and GABA and expressions of Glu and GABA, and normalize the excitotary/inhibitory metabolic imbalance of Glu/GABA through regulating Glu/GABA-Gln metabolic loop, which may be related to that Ningxin Anshen Fang has the curative effects of calming heart and tranquillizing mind, and sedation and hypnosis.

Objective To investigate the correlation of serum brain derived neurotrophic factor (BDNF),visceral adipose and cognitive dysfunction in elderly patients with type 2 diabetes.Methods From July 2016 to August 2017,72 patients with type 2 diabetes in our department of inpatient were selected as subjects.According to whether there was cognitive dysfunction,they were divided into 2 groups,type 2 diabetes mellitus combined with cognitive impairment group (A group) and simple type 2 diabetes group (B group).Another 40 healthy persons in the same period were selected as the control group.The indexes of glycolipid metabolism,visceral adipose and BDNF were measured in two groups.The logistic regression analysis was used to analyze the risk factors of cognitive dysfunction,and the relationship between visceral adipose,BDNF and cognitive function was analyzed by Pearson correlation.Results (1) The total cholesterol (TC),triglyceride (TG),low density lipoprotein cholesterol (LDL-C),fasting insulin (FINS),fasting blood glucose (FPG),glycosylated hemoglobin (HbA1c),fasting insulin (FINS),insulin resistance index (HOMA-IR),and visceral fat hormone level of the three groups were from high to low in A group ＞ B group ＞ C group (P ＜ 0.05);while the high density lipoprotein cholesterol (HDL-C),the expression level of BDNF and Montreal Cognitive Assessment (MoCA) score of the three groups were from high to low in C group ＞ B group ＞ A group (P ＜ 0.05);(2) The logistic regression analysis showed that visfatin,HOMA-IR,BDNF for the risk of cognitive impairment factors in elderly patients with type 2 diabetes;(3) Correlation analysis showed that the score and visceral fat MoCA and HOMA-IR were negatively correlated with the MoCA score was positively related to BDNF;HOMA-IR and visfatin was positively correlated,negatively correlated with BDNF.Conclusions The cognitive function of elderly patients with type 2 diabetes is related to serum visfatin and BDNF.Increased visfatin and BDNF may lead to cognitive dysfunction.

Objective To explore the effects of Xingpi Jieyu Fang (XPJYF, Spleen-invigorating Depression-relieving Formula) on behaviors and mitochondria ultrastructure of hippocampus neurons and skeletal muscle in depressed rats.Methods Wister rats were randomly divided into five groups: blank control group, model group, sertraline group, XPJYF high-dose and low-dose groups.Chronic unpredictable stress (CUS)was applied to establish depression models in rats for 28 days.Intragastric gavage was used to offer corresponding medication in treatment groups or distilled water in model and blank control groups.Weight change, sugar/water preference test and open-field test were used to evaluate depressive behavior in rats.Changes in ultrastructure of mitochondria in hippocampus neurons and skeletal muscles of depressive rats were measured by using transmission-electron microscope.Results Compared with the blank control group, weight gain in model group rats was significantly slowed while the sugar/water preference rate and total moving distance of open-field test decreased significantly (P<0.01).The number of mitochondria in hippocampal neurons and skeletal muscle in the model group was also reduced with irregular swelling, fractured and fuzzy cristae as well as vacuolar degeneration.under the transmission-electron microscope Compared with the model group, body weight, total sugar/water preference rate, and total moving distance of open-field test in XPJYF high-dose, low-dose group and sertraline group were significantly increased (P<0.01).No swelling or vacuolar degeneration were observed in mitochondria of hippocampal neurons and skeletal muscle in XPJYF high-dose group, which were close to the normal level.Hippocampal neuron and skeletal muscle mitochondria of XPJYF low-dose group and sertraline group were slightly swollen with partly fractured and fuzzy cristae.Conclusion The mitochondria structure of hippocampal neurons and skeletal muscle were damaged in depression induced by chronic stress, which may be a key factor in severe fatigue and symptoms involving multiple systems.Xingpi Jieyu formula could improve depressive behavior and mitochondria structure damage in depressed rats.To some extent, the effects of high-dose Xingpi Jieyu formula were better than sertraline in improving depression of rats.