Forty cases of unilateral low- frequency sensorineural hearing loss(LFSNHL) were analysed. The symptoms included tinnitus(87.5% ), deafness(75% ), vertigo(35%) and ear fullness(25% ). Audiome-try showed LFSNHL in all cases. The threshold difference between low frequencies and high frequencies was 26. 32 dB. The cause of LFSNHL, the relation between tinnitus, LFSNHL and endolymphatic hydrops were discussed. We hope that the diagnosis of LFSNHL should be standardized.

Vitamin D deficiency is highly prevalent in the general population including rheumatoid arthritis (RA) patients.Autocrine regulation of vitamin D modulates important immune response.Vitamin D deficiency may be involved in the pathogenesis of many autoimmune diseases including RA.Vitamin D regulates both the innate and adaptive immune responses.Vitamin D's effects on the innate immune system are mainly through the toll-like receptor and on the adaptive immune system through T cell differentiation,particularly the Helper T cell (Th) 17 response.As Thl7 cells are critical in the pathogenesis in RA,what effect will vitamin D deficiency lead to on the RA? Many data have indicated the relationship between vitamin D deficiency and RA disease activities,but not all the results are consistent.Here,we review the immune-modulatory roles of vitamin D and its effects on disease activity,osteoporosis and cardiovascular disease risk in RA.

AIM: To develop a simple HPLC for the determination of ursolic acid in Hawthorn leaves, and to compare ursolic acid content in Hawthorn leaves of different species, locations and growth stages, so as to supply some evidences for the exploitation and utilization of Hawthorn leaves reasonably. METHODS: By high-performance liquid chromatography method. Lichrospher C18 column (250 ×4.6 mm I. D. 5 μm); mobile phase, acetonitrile-water-orthophosphoric acid (85: 14.95: 0.05) with a flow-rate of 1.00 ml/min; column temperature at 30 ℃; injection volume, 5μl; UV detector at 210 nm. RESULTS: The detection limit (S/N=3) was less than 4. 024 μg/ml and the limit of quantification( S/N =10) was less than 12.05 μg/ml. The calibration curve showed good linear regression(r =0. 9999) within measurement ranges( 16.09 - 1030 μg/ml). The intra-day and interday variation were 0.71% and 6. 15%, respectively. The recoveries at low to high concentration were 89%-105%. Under these conditions, the ursolic acid content in different Hawthorn leaves were determined: 1.90%-1.95% in C. scabrifolia (Franch.) Rehd, 1.00%-1.45% in C. cuneata Sieb. & Zucc, 0.45%-0.65% in C.pinnatifida Bge. var. major N. E. Br.; In differnet growth stages of C. pinnatifida Bge. var. major N. E. Br. , the young leaves contain higher content of ursolic acid. CONCLUSION: The method is successfully applied to quantify ursolic acid in Hawthorn leaves. And the ursolic acid contents in Hawthorn leaves of differnent species are very different; C. scabrifolia (Franch.) Rehd contains the highest ursolic acid content in them. However, there is a little difference among different locations and growth stages for same species.

OBJECTIVE:To observe the curative effect of tazobactam sodium/piperacillin sodium for injection on the Neonatal infections.METHODS:140neonatal infections cases were randomly divided into treatment group and control group:the treated group were treated with tazobactam sodium/piperacillin sodium for injection100～200mg/(kg?d);while the con?trol group were treated with penicillin-G100000～200000IU/(kg?d).The course of therapy for both groups were5d～10d.RESULTS:The effective rates of the treatment group and the control group were91.43%,52.86%(P

AIM: To explore the clinical significance of detecting sCD44s and sCD44v6 in breast carcinoma.METHODS: Levels of serum soluble CD44 standard (sCD44s) and CD44 variant 6(sCD44v6) were detected by ELISA in 38 cases of breast cancer, 15 cases of benign breast diseases and 40 normal controls. RESULTS:The serum levels of sCD44s and sCD44v6 were significantly higher in patients with breast cancer than those with benign disease or normal controls. The serum concentrations of sCD44s and sCD44v6 in patients with stage Ⅲ, Ⅳ were significantly higher than those in patients with stage Ⅰ, Ⅱ(P

AIM: To determine the ultimate preservation time of murine cardiac grafts in 4℃ Ringer's solution. METHODS: Murine cardiac grafts were implanted to the abdominal vessels heterotopically 2, 4, 6, 8 and 10 hours after cold preservation. Graft survival rate and histological morphological changes, as well as the neutrilphil, T cell, macrophage infiltration, ICAM expression were determined by immunohistochemistry. RESULTS: The cardiac function-recovery rate and 1-week graft survival rate were 100% and 83.3% in 6-hour preservation group. Compared with non-preservation control group, no more apparent histological damages, cell infiltration and ICAM expression were found. CONCLUSION: The ultimate preservation time of murine cardiac graft in 4℃ Ringer's solution was 6 hours. [

Objective To construct and screen a hepatic stellate cells(HSCs) subtracted cDNA library, in order to seek differentially expressed genes in mice infected with Schistosoma japonicum. Methods HSCs were isolated from mouse as targets, and cDNA fragments of normal mouse were compared to those of S.japonicum infected mice to find differentially expressed genes by technique of suppression subtractive hybridization(SSH). Differentially expressed cDNA fragments were then directly inserted into T/A cloning vector to set up the subtractive library. Amplification of the library was carried out with transformation of DH5?. A subtracted cDNA library was constructed and then hybridized with forward and backward subtracted probes for differential screening. One hundred positive bacterial clones were randomly selected for sequencing and BLAST analysis. Finally, virtual Northern blot confirmed such differential expression. Results The amplified library contained more than 400 positive bacterial clones. Random analysis of 100 clones with restriction enzyme digestion showed that all clones contained 200-600 bp inserts. 76 ESTs were obtained with 70 related to fibrosis caused by schistosomiasis or other etiological factors. Other 6 ESTs were not found in PubMed. Conclusion The subtracted cDNA library of differentially expressed genes of HSC in normal mice and schistosome-infected mice was constructed successfully with SSH and T/A cloning techniques.

OBJECTIVE To study the possibility of applying Anerdian skin disinfectant for blood donor skin disinfection. METHODS Put Anerdian at 21℃,35℃ and 42℃ to exposure in air for 24h,48h and 72 h,detect the volatilization volume,effective iodine and appearance of disinfectant,and evaluate its effect on blood donor elbow by culture method. RESULTS At 21℃ exposure for 24 h the volatilization rate for Anerdian was 5%,effective iodine was 0.215%.At 35℃ and 42℃ exposure for 24 h its volatilization rate was more than 5%,and effective iodine was within the range of 0.18-0.22%.The average killing logarithm value of elbow skin natural bacteria in 30 donors was over 1. CONCLUSIONS Anerdian disinfectant is suitable for blood donor predonation skin treatment.

Objective To investigate the clinical effect of reserving gallbladder cholecystolithotomy by using laparoscopy com-bined with choledochoscopy in the treatment of gallbladder polyp and cholecystolithiasis .Methods The clinical data of 108 patients with gallbladder polyp complicating gallstone in our hospital were retrospectively analyzed .The patients were divided into 2 groups according to the operation modes .The treatment group adopted laparoscopy combined with choledochoscopy ,while the control group was treated by the traditional laparotomy .The clinical effect ,operation time ,postoperative out-of-bed time ,length of postop-erative hospital stay and occurrence rate of postoperative complications were compared between the two groups .Results The aver-age operation time had no statistical difference between the two groups (P>0 .05) .The average postoperative out-of-bed time and the time of postoperative hospital stay in the treatment group were significantly shorter than that in the control group (P<0 .05) . The occurrence rate of postoperative complications in the control group was higher than that in the treatment group (P<0 .05) . Conclusion The application of laparoscopy combined with choledochoscopy in reserving gallbladder choledochoscopy for treating gallbladder polyp and cholecystolithiasis has obvious effect and less complications ,and is worth being popularized in clinic .

Objective To investigate the inhibiting effect of interleukin-10 on the expressions of intercellular adhesion molecule-1(1CAM-1)and P-selectin in cerebral ischemia and reperfusion foci(I/R)in rats. Methods Sixty adult male Sprague-Dawley rats were assigned to 4 groups randomly:Sham operated group(n=6),cerebral I/R group(6,24,48 hours post I/R,each had 6 rats),vehicle group(6,24,48 hours post I/R,each had 6 rats)and IL-10 treatment group(6,24,48 hours post I/R,each had 6 rats).I/R rat model was established by middle cerebral artery occlusion(MCAO)with Longa's thread method. Immunohistochemical staining and retro-transcriptase polymerase chain reaction were used to detect the expressions of ICAM-1 and P-selectin. Results ICAM-1(31.60±1.85,54.11±2.32,48.61±2.30)and P-selectin(29.39±5.16,50.44±4.38,37.89±4.74)expressions on endothelial cells 6,24 and 48 hours post I/R were significantly upregulated as compared with sham group(7.61±1.14,0,respectively)(alI P＜0.01),and the expressions of ICAM-1 (24.06±2.42,42.00±3.26,45.28±2.91)and P-selectin(27.28±1.84,42.78±2.51,36.00±3.22)on endothelial cells of IL-10 treatment group 6h,24h,48 h post I/R were significantly suppressed at any timepoint in comparison with vehicle group(P＜0.05). Conclusions IL-10 could inhibit the expressions of ICAM-1 and P-selectin on endothelial cells of cerebral I/R foci in rats.