Objective To evaluate the mechanism of Fumai decoction in treating type 2 diabetes mellitus combined with acute cerebral infarction. Methods 68 cases of type 2 diabetes mellitus with acute cerebral infarction patients were divided into treatment group (34 cases) and western medicine control group (34 cases) at random. The serum levels of Endothelin (ET), Calcitonin gene related-pepitide (CGRP), Thromboxane-B2 (TXB2), 6-ketone-prostagla-ndins F1? (6-keto-PGF1?) and cerebral blood flow variation, clinical therapeutic effect were observed. Result Compared 2 groups, there was significant difference in improving neurological impairment, cerebral artery stenosis, cerebral circulation insufficiency and reducing levels of ET and TXB2, enhancing levels of CGRP and 6-keto-PGF1? (P0.05). Conclusion Fumai decoction can adjust the serum levels of ET, CGRP, TXB2 and 6-keto-PGF1?, and improve cerebrovascular function. Probably this is one of the mechanisms in treating type 2 diabetes mellitus with acute cerebral infarction.

Nerve mediated syncope is the most common type of syncope in children, which is a kind of functional cardiovascular disease, including vasovagal syncope(VVS), postural tachycardia syndrome(POTS), orthostatic hypotension(OH), orthostatic hypertension(OHT), situational syncope(SS)and carotid sinus syndrome(CSS). Among them, VVS and POTS are the main ones, accounting for about 95% of children with nerve mediated syncope.Nerve mediated syncope affects the children′s daily life, threatens their physical and mental health, and causes anxiety among the parents.Therefore, there have been lots of researches on this disease in recent years.This paper reviewes the research progress of clinical features, clinical diagnosis and treatment of VVS, POTS, OH and OHT in children and adolescents.

Objective: To explore the role of clinical pharmacist played in treating patients with acute myocardial infarction. Methods:The clinical pharmacists participated in the therapy for a patient with acute myocardial infarction and provided individual pharmaceutical care. The clinical pharmacist optimized the therapy by means of recognizing the drug-drug interactions and adverse drug reactions as well as evaluating the risk of thrombosis versus bleeding for the patient. Results:The therapeutic suggestions of the clinical pharmacist were accepted by the physicians. The patient was treated effectively and discharged from hospital. Conclusion: Clinical pharmacist can provide beneficial contributions to safe, effective and optimal medication.

Objective To compare the differential expression of MRP1 in human breast cancer sensitive cells (MCF-7/S)and resistant cells (MCF-7/TAM、MCF-7/ADR), and to preliminary distinguish whether there is a common mechanism between breast cancer cells resistant to tamoxifen (TAM) and to adriamycin(ADR).Methods We detected the expression of MRP1 gene and MRP1 protein in the three breast cancer cells by using the methods of real-time fluorescence quantitative PCR, and Western blot respectively.Results The expression of MRP1 gene in MCF-7/TAM and MCF-7/ADR cells was (2.63 ± 0.18) and (8.38 ±0.76) times of MCF-7/S cells respectively, which was significantly different (P1=0.004,P2=0.003). The expression of MRP1 protein in MCF-7/TAM and MCF-7/ADR cells was higher than MCF-7/S cells,which was consistent with the trend of MRP1 gene. Conclusions The high expression of MRP1 may be the common mechanism in breast cancer cells resistant to TAM and ADR.

Objective To observe the influence of chlorderazin on the change of L-arginine transport in human erythrocytes induced by oxidized low density lipoprotein. Methods Human erythrocytes were obtained from six healthy donors. The study included three groups: Gr1 (control)? Gr2 (incubated with OX-LDL) and Gr3 (incubated with OX-LDL and chlorderazin, a PLA 2 inhibitor). The 3H-L-arginine transport was measured.Results Compared with Gr1, the Vmax for the total transport of L-arginine in erythrocytes in Gr2 decreased by 33%, and Km value increased. Compared with Gr2, the Vmax for the total transport and the transport via system y+ of L-arginine in erythrocytes in Gr3 increased by 37% and 25% respectively, and Km values decreased for both. But the change of L-arginine transport by system y+L was not statistically significant.Conclusion OX-LDL could inhibit L-arginine transport in human erythrocytes, which could be reversed by chlorderazin, a nonspecific PLA 2 inhibitor.

Objective To evaluate the in vivo efficacy of modified platelet(PLT) additive solution(PAS-ⅢM) with trehalose as a substitute for plasma for the storage of platelet concentrates at low temperature(10℃).Methods Rabbit platelet concentrates were collected and stored in different media and temperature conditions,plasma(22℃),70%PAS-ⅢM/30% plasma(10℃) and plasma/trehalose(-85℃).Platelets in plasma(22℃) on storage day 3,in 70%PAS-ⅢM/30% plasma(10℃) on storage day 3,7,9,and in plasma/trehalose(-85℃) thawed on day 20 were transfused to rabbit thrombocytopenia model.The in vivo recoveries and survivals of the three preserved platelets and fresh platelets were measured and evaluated after transfusion into rabit model of thrombocytopenia.Results The survival of platelets stored in 70% PAS-ⅢM/30% plasma(10℃) on day 9 was lower than that of fresh platelets(P0.05).Recovery and survival of frozen platelets were significant lower than those of the other groups(P

Forty cases of unilateral low- frequency sensorineural hearing loss(LFSNHL) were analysed. The symptoms included tinnitus(87.5% ), deafness(75% ), vertigo(35%) and ear fullness(25% ). Audiome-try showed LFSNHL in all cases. The threshold difference between low frequencies and high frequencies was 26. 32 dB. The cause of LFSNHL, the relation between tinnitus, LFSNHL and endolymphatic hydrops were discussed. We hope that the diagnosis of LFSNHL should be standardized.

ObjectiveTo investigate the diagnosis and treatment of occult thyroid carcinoma(OCT).MethodsRetrospective analysis on the clinical data of 34 cases of OCT was made. ResultsOnly 6 cases were preoperatively diagnosed as OCT. Total resection of the affected lobe and isthmus was performed on 16 cases with neck dissection in 14 cases. Subtotal thyroidectomy was performed on 18 cases. The occult cancer lesion was pathologically confirmed within ipsolateral lobe in all the 34 cases. Neck lymph node metastasis was found in 4 cases and local infiltration in 3 cases. The psammoma bodies in cancer lesion and adjacent normal gland were found in 15 cases. Thirty-one cases were followed up for an average of 9 years. One died of lung metastasis and two more of other unrelated diseases. Conclusion(1) The psammoma bodies in normal thyroid gland usually imply the existence of cancer. (2) Total thyroidectomy of a lobe or subtotal thyroidectomy was the therapy of choice. Local infiltration or neck lymph node metastasis necessitates additional neck dissection.

Objective To investigate the relationship between polymorphisms of candidate genes 〔glucokinase (GCK) gene, NIDDM1(D2S140) gene, fatty acid bindingprotein2(FABP2)gene〕and type 2 diabetes mellitus (DM) in Shandong Chinese Han population. Methods Short tandem repeat (STR) region polymorphisms of GCK gene, NIDDM1 gene and FABP2 gene were analyzed with the polymerase chain reaction and polyacrylamide sequencing gel in 107 patients with type 2 diabetes mellitus and 105 normal subjects. The subjects studied were unrelated and they are Chinese Hans living in Shandong province and no diabetes family history was revealed. Results A5 allele of GCK gene and B2 allele of NIDDM1 gene were positively related with type 2 diabetes (RR=15.70, P0.05). Logistic regression analysis showed that A5 allele and B2 allele were among the risk factors of type 2 diabetes. Conclusion Polymorphic microastellite repeat markers at the glucokinase gene and the NIDDM1 (D2S140) gene locus are associated with type 2 diabetes in Chinese. The polymorphism at the FABP2 locus is not a genetic marker for type 2 DM in Chinese Hans.

Objective To determine the relationship between the polymorphism of D2S140 gene and type 2 diabetes mellitus.Methods D2S140 region polymorphism were analyzed with the polymerase chain reaction and short tandem repeat methods in 103 subjects with type 2 diabetes mellitus and 105 nondiabetic subjects.All subjects were biologically unrelated Chinese without a family history of DM.Results There were 7 alleles ranging from 151bp to 163bp in all subjects.The frequencies were 0.01 to 0.37,2 alleles were more common in diabetic patients than in nondiabetic subjects (RR=16.04, P