Objective To evaluate the mechanism of Fumai decoction in treating type 2 diabetes mellitus combined with acute cerebral infarction. Methods 68 cases of type 2 diabetes mellitus with acute cerebral infarction patients were divided into treatment group (34 cases) and western medicine control group (34 cases) at random. The serum levels of Endothelin (ET), Calcitonin gene related-pepitide (CGRP), Thromboxane-B2 (TXB2), 6-ketone-prostagla-ndins F1? (6-keto-PGF1?) and cerebral blood flow variation, clinical therapeutic effect were observed. Result Compared 2 groups, there was significant difference in improving neurological impairment, cerebral artery stenosis, cerebral circulation insufficiency and reducing levels of ET and TXB2, enhancing levels of CGRP and 6-keto-PGF1? (P0.05). Conclusion Fumai decoction can adjust the serum levels of ET, CGRP, TXB2 and 6-keto-PGF1?, and improve cerebrovascular function. Probably this is one of the mechanisms in treating type 2 diabetes mellitus with acute cerebral infarction.

OBJECTIVETo construct a GFP/Puro double-labeled lentiviral expression vector for CK8 silencing and assess the effects of CK8 silencing on cell apoptosis.

METHODSThe siRNA sequences of CK8 were inserted into the lentiviral expression vector GV248 and transfected into 293T cells with the packaging plasmids PMD and SPA. The lentivirus was collected at 24 and 36 h post-transfection. Flow cytometry was used to detect the virus titer and the positive cells were selected with puromycin. The knockdown of CK8 was examined by Western blotting. The effect of CK8 down-regulation on cell apoptosis induced by cisplatin was detected with Annexin V/PI staining.

RESULTS AND CONCLUSIONWe successfully constructed CK8 interference lentiviral vector and obtained a stable cell line with CK8 knock-down that was sensitive to cisplatin-induced apoptosis.

Apoptosis ; Cell Line ; Down-Regulation ; Genetic Vectors ; Humans ; Keratin-8 ; genetics ; Lentivirus ; Plasmids ; RNA Interference ; RNA, Small Interfering ; Transfection

Objective To investigate the effect of Genistein,a tyrosinase inhibitor,on retinal neovascularization in mice.Methods Thirty-six 7-day-old C57BL/6J mice were randomly assigned into hyperoxiainduced group,Genistein group,DMSO group and normal control group.The mice in the hyperoxia-induced group,Genistein group and DMSO group were fed in a static chamber with the oxygen volume fraction (75±2)％ for 5 days and then sent back to natural environment for 5 days to establish retinal neovascular models,and 1 μl Genistein diluted by 5％ dimethyl sulfoxide (DMSO) (400 mg/L) and 1 μl DMSO were intravitreally injected in the 12-dayold mice of Genistein group and DMSO group,respectively.The mice in the normal control group were bred in natural environment.The fluorescence angiography was carried out in 17-day-old mice (2 mice in each group) to prepare the whole retinal flatmounts,and the morphology of retinal vessels was observed under the fluorescence microscope.The other mice in various groups were sacrificed and the retinas were collected.The expressions of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in mRNA and protein levels were detected by realtime fluorescence quantitative PCR and Western blot,respectively.The use and care of the mice complied with regulations for the management of laboratory animals.Results Retinal vessels were normal in the mice of normal control group.In the mice of the hyperoxia-induced group and DMSO group,retinal vessels were tortuous,and neovacularization and non-perfusion areas were visible.In the Genistein group,retinal vessels were clearly visible,but non-perfusion areas were exhibited.The relative expression levels of VEGF mRNA in retinas were 0.64±0.25,0.37±0.23,0.03±0.02 and 0.04±0.02,and the relative expression levels of bFGF mRNA in retinas were 21.40±3.07,17.22±2.63,0.52±0.25 and 0.67±0.23,in the hyperoxia-induced group,DMSO group,Genistein group and normal control group,and the expressions of VEGF and bFGF in mRNA level in the hyperoxia-induced group and DMSO group were significantly higher than those in the Genistein group and normal control group (all at P＜0.05).The protein expression levels of VEGF and bFGF were 1.01 ±0.05 and 0.97±0.06 in the hyperoxia-induced group,1.06±0.07 and 1.03±0.08 in the DMSO group,0.73±0.05 and 0.76±0.07 in the Genistein group,0.52±0.05 and 0.56± 0.05 in the normal control group.The expressions of VEGF and bFGF in protein level in the hyperoxia-induced group and DMSO group were significantly higher than those in the Genistein group and normal control group (all at P＜0.05).Conclusions Genistein can inhibit hyperoxia-induced retinal neovascularization may be by downregulating the expressions of VEGF and bFGF in retinas.

Objective To construct a GFP/Puro double-labeled lentiviral expression vector for CK8 silencing and assess the effects of CK8 silencing on cell apoptosis. Methods The siRNA sequences of CK8 were inserted into the lentiviral expression vector GV248 and transfected into 293T cells with the packaging plasmids PMD and SPA. The lentivirus was collected at 24 and 36 h post-transfection. Flow cytometry was used to detect the virus titer and the positive cells were selected with puromycin. The knockdown of CK8 was examined by Western blotting. The effect of CK8 down-regulation on cell apoptosis induced by cisplatin was detected with Annexin V/PI staining. Results and Conclusion We successfully constructed CK8 interference lentiviral vector and obtained a stable cell line with CK8 knock-down that was sensitive to cisplatin-induced apoptosis.

Objective To construct a GFP/Puro double-labeled lentiviral expression vector for CK8 silencing and assess the effects of CK8 silencing on cell apoptosis. Methods The siRNA sequences of CK8 were inserted into the lentiviral expression vector GV248 and transfected into 293T cells with the packaging plasmids PMD and SPA. The lentivirus was collected at 24 and 36 h post-transfection. Flow cytometry was used to detect the virus titer and the positive cells were selected with puromycin. The knockdown of CK8 was examined by Western blotting. The effect of CK8 down-regulation on cell apoptosis induced by cisplatin was detected with Annexin V/PI staining. Results and Conclusion We successfully constructed CK8 interference lentiviral vector and obtained a stable cell line with CK8 knock-down that was sensitive to cisplatin-induced apoptosis.

Objective:To explore the mediating effect of social support on social avoidance and distress and reproductive concerns in young breast cancer patients undergoing chemotherapy.Methods:This is a cross-sectional study. From February 2020 to December 2021, a total of 180 young breast cancer patients undergoing chemotherapy in Shandong Cancer Hospital were selected as the research objects using the convenient sampling method. General Data questionnaire, Social Support Rating Scale, Reproductive Concerns After Cancer Scale (RCAC) and Social Avoidance and Distress Scale (SADS) were used to investigate patients. Pearson correlation analysis was used to investigate the correlation among social support, social avoidance, distress and reproductive concerns in young breast cancer patients undergoing chemotherapy. Structural equation models was used to explore the mediating effect of social support between social avoidance and distress and reproductive concerns. A total of 180 questionnaires were distributed in this study, and 172 were effectively received, with an effective recovery of 95.56% (172/180) .Results:The total score of SADS of young breast cancer patients undergoing chemotherapy was (18.98±3.15), the total score of RCAC was (59.85±5.03), and total score of Social Support Rating Scale was (33.53±4.25). Pearson correlation analysis results showed that social avoidance, distress was positively correlated with reproductive concerns ( r=0.810, P<0.01), and social support was negatively correlated with reproductive concerns and social avoidance and distress ( r=-0.570, -0.612; P<0.01). Structural equation model results showed that social support played a partial mediating role between social avoidance and distress and reproductive concerns. Conclusions:Social support plays a mediating effect between social avoidance and distress and reproductive concerns in young breast cancer patients undergoing chemotherapy. Medical and nursing staff should provide more social support for young breast cancer patients undergoing chemotherapy, reduce reproductive concerns and improve quality of life.