A consecutive series of 37 eyes of 34 glaucoma patients treated by transillumination guided cyclocryo- therapy(CCT)either to obtain pain relief or to achieve IOP control in Helsinki University Eye Hos- pital was studied.The follow-up range was 1～43 months(mean 9 months).Pretreatment IOP was 38?14mm Hg and postoperative 27?17mm Hg(mean ?SD).Twenty-four eyes underwent one and 13 eves 2～5 procedures.IOP control(IOP 9～24mm Hg) was achieved in 51%.The response was more favo- rable in eyes without iris neovascularization(62% achieved lOP control)than in eyes with neovascular glaucoma(38% achieved IOP control).The best response was achieved in eyes with chronic uveitis (6/7 of eyes achieved lOP coutrol).Repeated proce- dures improved IOP control rate.Thirteen eyes needed primarily pain relief and 85% achieved it.Postope- rative vision was unchanged in 30% and became worse in 54%.Transillumination guided the applica- tion of the cryoprobe to the correct site.Hypotony (IOP

Six human epikeratophakia lenticules were remo- ved 1 to 23 months after refractive keratoplasty for aphakia(2)and myopia(3).The myopic group in- cluded one epi-lens treated with ArF-excimer laser for hyperopia.Causes of removal were epithelial de- fect(4),edema of host cornea(1)and overcorre- ction(1),The epi-lens stroma showed a central regular fibrillar structure with a low keratocyte den- sity.Electronmicroscopy revealed preserved cytoplas- mic organelles and nuclear appearance.Lymphocytes and other mono-nuclear cells were absent,Bowan's membrane was normal in all cases.Breaks were not seen while a slight wavy appearance and occasionally focal bends occurred.The epithelium was somewhat irregular and changing in thickness.In the peripher- al wound region stromal and epithelial irregularities were evident.

The following indexes of 2, 10 and 24 months old wistar rats were measured. the activities of RBC membrane Na+-K+-ATPase, RBC superoxide dismutasc (SOD), blood glutathione. peroxidase (GSH-Px), and the levels of lipid peroxides (EPO) in plasma and RBC membrane From 2 to 10 month-old animals, increases of 24.16%,40.07%, 102.7% and 44.44%in Na-K-ATPase activity, GSH-Px activity, plasma LPO and RBC membra no LPO levels, respectively, were found (P

By means of radioimmunoassay, leucine-enkephalin (L-ENK) in the uteri of women and rats was studied and its responses to ovarian steroids were observed. The histological localization of L-ENK and the influence of naloxone (NAL) on estrogen regulation of the rat uterus in terms of cAMP and eGMP determination were also studied. Results of the study demonstrated that presence of L-ENK like substance in the uteri of women and rats, and the concentrations varied in the various phases of the sexual cycle. indicating a positive correlation with levels of estrogen in the body. L-ENK was found localized in the interstetial cells of the human endometrium, and in the rat uterus. Effect of extrogen on nucleotide was influenced by NAL. The findings suggested that L-ENK may play an important role on physiologic function of utetus.

0.05). Conclusion The difference of cross sectional area in nasal cavity resulted from nasal septum deviation. Compensatory hypertrophy of inferior turbinate, uncinate process and middle turbinate in wide side resulted in the similar proportion of nasal airway of two sides of nasal cavity. However, compensatory hypertrophy lead to obstruction and stricture, accordingly, the probability of unilateral inflammatory maxillary sinus diseases in wide side of nasal cavity was higher than that in narrow side. In clinical practice, the deviated nasal septum should be corrected at the time of dealing with inflammatory maxillary sinus diseases. The normal structure and function of nasal cavity were recovered. The decompensation due to septum deviation was blocked.

Objective To study the out come of coblation arytenoidectomy combined with true and false pos-terior cordectomy for treatment of bilateral vocal cord paralysis (BVFP) .Methods The group included 17 patients (8 incomplete BVFP and 9 complete BVFP) .All the patients had waited more than 6 months before surgery with no spontaneous recovery .The patients underwent arytenoidectomy combined with true and false posterior cordecto-my using coblation under video suspension laryngoscope .Electric fibrolaryngoscope was performed before and after operation to evaluate the size of the glottis .The patients'voices were recorded before and after operation and evalua-ted by 3 laryngologists through GRBAS .Results Preoperational laryngoscope showed that in the incomplete BVFP ,the vocal folds were close to the middle line with limited abduction .In the complete BVFP ,the vocal folds failed in adduction and abduction and the size of the glottis was 2 -3mm .3 of the patients had underwent tracheotomy . Post operational electric fibrolaryngoscope showed proximate triangulate breathing space which was more than 4mm in the posterior glottis .The patients were followed up 6 to 44months .15 of the 17 were allowed for decannulation . One patient among them underwent posterior cordectomy and arytenoidectomy on the contralateral side respectively after the first operation because of dyspnea owing to scar diathesis .Two patients bunged up the canula but did not de-cannulate .Among them one was a 8-year old boy diagnosed as congenital heart disease .One was thyroid cancer with stiff neck due to radiology .The only postoperative complication was the appearance of granulations in the operation region in 2 patients ,which were disappeared spontaneously .Quality of the voice improved in 2 patients ,unchanged in 10 and worsened in 5 .Conclusion This study demonstrates that arytenoidectomy associated with posterior cord-ectomy is a satisfactory surgical treatment of bilateral vocal fold paralysis because it leads to a considerable and stable enlargement of the breathing space and preserved the voice quality .

Objective To evaluate the efficacy of recessive placement of nasolacrimal stent in the treatment of nasolacrimal duct obstruction and factors affecting the prognosis of the procedure.Design Retrospective case series.Participants One hundred eighty six patients(207 eyes) with nasolacrimal duct obstruction.Methods One hundred eighty six patients(207 eyes) of nasolacrimal duct obstruction confirmed by dacryocystography had undergone recessive placement of nasolacrimal stent after probing and dilation of nasolacrimal duct.All patients received regular postoperative nasolacrimal duct syringe.Main Outcome Measures Status of postoperative lacrimal syringe.Results The stents were removed 3～12 months postoperatively,the patients were followed-up average 6 months.The operation success rate was 97.1%.167 eyes achieved complete success(80.7%).25 eyes became better(12.1%),and nasolacrimal duct obstructed again in 15 cases(7.2%).Conclusions Recessive placement of nasolacrimal stent combined with postoperative nasolacrimal duct syringe is a simple,effective and safe technique in the treatment of nasolacrimal duct obstruction.Selecting correct patients,skillful technique of nasolacrimal duct probing and regular postoperative syringing of nasolacrimal duct are the main factors of successful procedures.

Objective To investigate the efficacy and safety of hydrogel soft contact lens(HSCL) for persistence corneal epitheliopathy after intraocular surgery.Design Retrospective case series.Participants 36 patients(36 eyes) with bullous keratopathy(n=5) or other keratopathy(n=31) including corneal erosion,epithelium missing,and dendritic keratopathy after intraocular surgery,which failed to medicine treatment 2 weeks ago.Method All patients were fitted with HSCL after suitable processing of the corneal foci.The lenses were removed immediately as long as the keratopathy recovered.A new lens was replaced after 3 weeks if still needed.Main Outcome Measures Changes of keratopathy,pain relief and complications.Result 35 patients(97.22%) fitted with HSCL were very comfort within 3 days.At the follow-up of 6 months,30 patients(83.33%) achieved complete remission of keratopathy,6 patients (16.67%) remarkable remission.30 patients(86.11%) had vision increase.All of the non-bullous keratopathy recovered within 3 weeks,while the lenses were fitted much longer in bullous keratopathy.No other lens-related complications appeared.Conclusion The application of hydrogel soft contact lens to persistence corneal epitheliopathy after intraocular surgery is safe,effective,and practical.

Objective To analyze the mutations of candidate WFS1 gene in the Chinese pedigree with Low frequency sensorineural hearing loss. Methods PCR were performed with fourteen pairs of primers in the coding sequence of WFS1 gene. The PCR products were subsequently sequenced in the 38 individuals of LFSNHL family for screening the gene mutations. Results The PCR amplification fragments showed well quality in the five pairs of primer, and further analysis with frequencing showed no polymorphism and mutations in the members. Conclusion The present study rule out the possibility that the deafness gene WFS1, which locates on the 4p16, lead up to the hearing loss of LFSNHL pedigree.

For screening the potential drugs as anti-liver fibrosis candidates, we established a high- throughput drug screening cell model based on COL1A1 promoter. The activity of COL1A1 promoter and luciferase reporter gene can be elevated by TGF-β1, and inhibited by candidate drugs. We constructed a recombined plasmid with COL1A1 promoter and luciferase reporter gene pGL4.17, the activity of COL1A1 promoter was reflected by fluorescence intensity. COL1A1 promoter activity was detected by Dual-Luciferase Reporter Assay System, it came that the relative luciferase activity of COL1A1 promoter was 15.98 times higher than that of control group induced by TGF-β1, showing the recombined plasmid could be used in cell model. The recombined plasmid was transfected into human hepatic stellate cells LX2, detected the effect of potential drugs, and obtained a stable expression system through stable transfection and monoclonal cell culture. A sample which could reduce COL1A1 promoter activity signally by our cell model, decreased collagen I mRNA and protein expression detected by real-time RT-PCR and Western blotting. It indicates this novel cell model can be used in high-throughput drug screening of potential anti-liver fibrosis drugs.