OBJECTIVE:To establish an RP-HPLC method for the determination of Berberine hydrochloride in Keyang-min tincture.METHODS:The sample separation was performed on Agilent Eclipse XDB C18(250 mm?4.6 mm,5 ?m),and the mobile phase was acetonitrile-5 mmol?L-1 perfluorooctane sulfonate(32∶68) at flow rate of 1 mL?min-1.The detection wavelength was 346 nm and the temperature of column was 35 ℃.RESULTS:The linear range of Berberine hydrochloride was 0.059 2～0.888 3 ?g(r=0.999 9).The average recovery rate was 99.71%(RSD=1.18%,n=6).CONCLUSION:The met-hod is sensitive,accurate,and reproducible,and suitable for the determination of Berberine hydrochloride in Keyangmin tincture.

OBJECTIVE:To study the effect of Hordeum vulgare on hormone level in male mice.METHODS:Male mature mice(5～6 months) were treated with Hordeum vulgare decoction 1 mL(equivalent to 0.5 g?mL-1 crude drug) bid for 14 consecutive days.Follicle-stimulating hormone(FSH),luteinizing hormone(LH),estradiol(E2) and testosterone(T) in blood were monitored.RESULTS:In Hordeum vulgare group compared normal control,serum E2 increased significantly,serum level of testosterone decreased significantly,while FSH and LH levels remained stable.CONCLUSION:Hordeum vulgare can influence the hormone level in male mice by influencing the gonad axis of the mice.

0.05); the effective rates in rest pain in the two groups were 41.25% and 57.97%,respectively(P

Antineoplastic Agents ; therapeutic use ; Benzamides ; Drug Resistance, Neoplasm ; Exons ; Gastrointestinal Stromal Tumors ; drug therapy ; metabolism ; pathology ; Humans ; Imatinib Mesylate ; Mutation ; Neurofibromatosis 1 ; metabolism ; pathology ; Omentum ; Peritoneal Neoplasms ; metabolism ; pathology ; Piperazines ; therapeutic use ; Prognosis ; Proto-Oncogene Proteins c-kit ; genetics ; metabolism ; Pyrimidines ; therapeutic use

Antibodies, Monoclonal ; therapeutic use ; Antibodies, Monoclonal, Humanized ; Antineoplastic Agents ; therapeutic use ; Antineoplastic Agents, Hormonal ; therapeutic use ; Antineoplastic Agents, Phytogenic ; therapeutic use ; Breast Neoplasms ; drug therapy ; pathology ; ultrastructure ; Carcinoma, Ductal, Breast ; drug therapy ; pathology ; ultrastructure ; Chemotherapy, Adjuvant ; Female ; Humans ; Neoadjuvant Therapy ; Nitriles ; therapeutic use ; Taxoids ; therapeutic use ; Trastuzumab ; Treatment Outcome ; Triazoles ; therapeutic use

Objective To find out the status of nosocomial infection management in hemodialysis sections, in order to encourage their continuous quality improvement and standardize their management Methods 41 hemodialysis sections with (≥) 3 dialyzers and independent operation were chosen for investigation. On-site investigation and environmental microbiology monitoring combined. Results The higher the level of the hospital, the better the nosocomial infection control in its implementation of the infection control measures. In terms of rules and regulations, incompliant secondary hospitals and private hospitals accounted for 56. 2% of hospitals of the same ranking; in terms of layout and processes, 75% are found incompliant; and various defects are found in the dialyzer reuse management and disinfection in tertiary and lower hospitals. As to hand hygiene of the staff, the incompliance rate is over 55%. As indicated in the results of microbial environment monitoring, the compliance rate of dialysis water is over 90%, that of dialysate is 78.6% ～ 86.2%, and that of hand disinfection is 56.6%～78.6%.Conclusion It is imperative to strengthen the supervision of secondary hospitals and private hospitals,and to train their staff in the knowledge of hand hygiene and nosocomial infection in hemodialysis rooms.Hospitals found with poor infection management will be encouraged to take issue-specific actions for rectification.

Aim To establish and evaluate a mouse model of my-ocardial fibrosis by hypodermic injection of isoprenaline. Meth-ods Kunming mice were randomly divided into 2 groups, namely, the myocardial fibrosis model group and the control group, 10 mice in each group. The mice in the model group were given isoprenaline 5 mg · kg-1 by hypodermic injection. From the following day, the dose of isoprenaline was reduced to 2. 5 mg·kg-1 , and lasted for 30 days. The mice in the control group were treated with the physiological saline in the same way. Final-ly, heart weight was then weighed and the cardiac weight index (CWI) was calculated. Hydroxyproline (Hydro) level in myo-cardium was determined by a colorimetric method. HE and Mas-son’s trichrome staining were used to estimate the extent of myo-cardial fibrosis and calculate the collagen volume fraction ( CVF) . RT-PCR was used to measure the myocardial Collagen I mRNA expression. Results Compared with the control group, the CWI and Hydro content in the myocardial tissues in the model group were increased(P<0.01). The content of col-lagen in the myocardial tissues and the CVF were increased obvi-ously(P<0.01). The RT-PCR results showed that the left ven-tricle Collagen I mRNA expression in the model group increased obviously( P<0.01 ) . Conclusion Isoprenaline-induced myo-cardial fibrosis model has been established and the method is very simple, economic and reliable.

In this study, a literature review was adopted to specify terminology of biosimilars and demonstrate the basic characteristic of biologics and relevant research and development ( R&D) procedures. The regulatory frame-work of the European Medicines Agency ( EMA ) guidelines on biosimilars was introduced. Explicitly, regulatory guidelines and scientific principles, regarding biosimilarity, safety and immunogenicity, extrapolation, labels and names, data protection, were systematically introduced, as well as interchangeability and pharmacovigilance, respec-tively. The purpose of the study is to provide regulatory references for Chinese legislators and recommendations on the R&D of biosimilars in the biopharmaceutical industry.

Objective To investigate the therapeutic effects, duration of hematopeietic depression and side effects of FLAG regimen for refractory and relapsed acute myeloid leukemia(AML). Methods 13 cases for 5 days, and granulocyte colony stimulating factor G-CSF 300 μg/d from day 0 till neutruphil recovery (ANC≥1x109/L). 13 patients were 8 males and 5 females. The median age was 41.8 years(range 28-67). It includes 5 relapsed AML patients and 8 refractory AML patients. Results Of the 17 times, 11 showed response to therapy; the rate of complete remission was 35.3 %(6/17); the rate of partial remission was 29.4 % (5/17) and the overall response rate was 64.7 %. Main toxicities were gastrointestinal side effects, myelosupression and the function of liver was lightly damaged. Neutropenia at lowest number began at the time of 5-12 days after regimen initiating and last 7-34 days, thrombocytopenia began at the time of 5-13 days and last 8-30 days. Conclusion FLAG regimen showed obvious effects for refractory and relapsed AML Most patients gained chance for hematopoietic stem cell transplantation. Hemotological and nonhemotological toxicities are acceptable.

Objective: To probe into the relationship between changes of sex hormone levels and TCM syndrome differentiation in climacteric women.Methods: According to TCM syndrome differentiation,55 cases of women in menopause were divided into three groups: kidney yang deficiency,kidney yin deficiency and the normal;the correlation between the three types and E2,FSH and LH was explored by statistical methods,and then they were compared with procreative women.Results: In all the 55 cases,kidney yin deficiency covered 70.90%,kidney yang deficiency covered 16.36%,the normal covered 12.72%;changes of E2 correlated positively with symptom scores of kidney yang deficiency and kidney yin deficiency;changes of FSH and LH correlate negatively with that of kidney yang deficiency and kidney yin deficiency.And they all correlated closely with menopause.Conclusion: the main syndrome type of climacteric women was kidney yin deficiency;E2↓,FSH↑ and LH↑ was expression of physiological kidney deficiency of climacteric women.