1.Protection of neurons in vitro and improvement of learning and memory in mice by 2-phenoxy-indan-1-one derivatives
Fengyang CHEN ; Xiaoliang ZHENG ; Rong SHENG ; Zhong CHEN ; Hong SHI
Chinese Pharmacological Bulletin 1987;0(03):-
Aim To study the effect of novel AChE inhibitors, 2-phenoxy-indan-1-one derivatives (YKY-1~7), against glutamatic acid-induced neurotoxicity in PC12 cells and on learning & memory impairment in dementia model mice induced by A?25~35 icv Methods The PC12 cells were preincubated with different concentrations of YKY-1~7 for 24 h and subsequently treated by glutamatic acid, at the high concentration of 2 mmol?L-1 for 15 min to induce cytotoxicity. The cell viability was assessed with MTT method.. Dementia model mice were made by intracerebroventricular injection (icv) of aggregated A?25~35. From the next day, the model mice were administered YKY-7 (2.5, 5, 10 mg?kg-1, ig) for 10 consecutive days and sham control mice or A? model control mice received daily ig saline. After the final treatment, the passive avoidance learning was tested, regional cerebral blood flow at cerebral cortex was assessed, and the activity of AChE in the cerebral cortex, hippocampus and blood serum were determined. Results Six out of the seven YKY compounds appeared to be effective against glutamatic acid-induced neurotoxicity in PC12 cells, with YKY-7 demonstrating the most activity. YKY-7 significantly ameliorated the learning and memory ability in dementia model mice induced by A?25-35 icv, slightly and selectively inhibited the cortical and hippocampal AChE, and gently increased the blood flow at cerebral cortex. Conclusion Some of 2-phenoxy-indan-1-one derivatives reported here have protective effects against glutamatic acid induced neurotoxicity in PC12 cells, and improve the learning and memory impairment induced by A?25-35, which may be partly attributable to its selective inhibition of AChE activity in the cerebral cortex and hippocampus.
2.The manipulation of turn and sway for the treatment of special kind of Galeazzi fracture in youngsters.
Xiao-rong ZHENG ; Xiao-bao WU ; Zhen-sheng WANG
China Journal of Orthopaedics and Traumatology 2011;24(11):958-959
Adolescent
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Child
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Humans
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Male
;
Manipulation, Orthopedic
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methods
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Radius Fractures
;
therapy
3.Preliminary analysis of bitter substances in spica of Prunella vulgaris.
Xin ZHAI ; Meng-Qian XI ; Qiao-Sheng GUO ; Huan-Huan HAN ; Xiang ZHANG ; Wei YANG ; Rong-bo ZHENG ; Xiao-Dan HUANG ; Huan-Rong ZHU
China Journal of Chinese Materia Medica 2014;39(3):423-426
Volatile oil components and the contents and types of amino acid in spica of Prunella vulgaris were analysed by GC-MS and amino acid analyzer. Esters, fatty acids, aromatic hydrocarbon, ketone and several alcohol compounds were identified by mass spectrum comparison. In these ingredients, beta-ionone smelled aroma of cedar, raspberry, nerolidol showed weak sweet soft orange blossom flavor, neroli tasted sweet and fresh, nerolidol tasted sweet with light aroma of wood, hexadecanal showed a weak aroma of flowers and wax, alpha-sinensal had rich and fresh sweet orange flavor. To some extent, these types of aromatic substances can affect the taste of herbal tea or decoction made of Spica Prunellae. Among amino acids detected, natural amino acids accounted for a larger proportion, and those natural amino acids showed bitterness, slight bitterness, sourness (freshness), sweetness, slight sweetness, sourness (slight freshness). The results indicated that bitter and slightly bitter amino acids have the greatest impacts on the sense of Spica Prunellae.
Amino Acids
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analysis
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Gas Chromatography-Mass Spectrometry
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Oils, Volatile
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analysis
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Prunella
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chemistry
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Taste
4.Association of Claudin-1,Ki-67,and CD34 expressions with astrocytic tumor cell proliferation and invasion
Yong-Lian WU ; Guo-Rong WANG ; Sheng ZHANG ; Jian-Sheng ZHENG ; Yu-Peng CHEN
Chinese Journal of Neuromedicine 2008;7(10):1023-1026
Objective To investigate the correlation ofClaudin-1, Ki-67 and CD34 expressionsin the tumor tissue to the invasiveness, grading and proliferation of astrocytic tumors. Methods In 50cases of astrocytic tumor, the expressions of Claudin-1, IO-67 and CD34 were detectedimmunohistochemically in the tumor tissue, normal brain tissues and in the junctional area between thetumor and normal tissues. The expressions of Claudin-1, Ki-67, and CD34 were analyzed in relation tothe clinicopathoiogical characteristics of the patients. Results The expression rate of Claudin-1 was70.59% in the astrocytic tumors of histological grades Ⅰ and Ⅱ, significantly higher than that in grade Ⅲand Ⅳ tumors (9.09%, χ2=20.206, P=0.000). In grade Ⅲ and Ⅳ tumors, the positivity rates of Ki-67 inthe normal tissues, junctional area and the tumor tissue were all significantly higher those in grade Ⅲ andⅣ tumors (t=9.144, 5.958, and 6.297; P=0.000, 0.000, and 0.000, respectively). Grade Ⅲ and Ⅳ tumorsdisplayed significantly greater mierovessel density (MVD) than grade Ⅰ and Ⅱ tumors in the tumor tissue(t=3.101, P=0.003) and the junctional area (t=4.139, P=0.000). In all the tumors, significantly higherKi-67 expression was seen in the tumor tissue (12.74±6.93) than in the junctional area (7.42±3.93) andthe normal tissue (3.22±1.57) (F=51.726, P=0.000), and the MVD was also greater in the tumor tissue(27.48±8.26) than in the junctional area (10.72±3.93) and normal tissue (6.48±1.45) (F=215.538, P=0.000).Conclusion Expressions of Claudin-1, Ki-67, and CD34 are closely correlated to the proliferation andinvasion of astrocytic tumors, and these cytokines may serve as important indicators for evaluating themalignancy of astrocytic tumors..
5.Simultaneous determination of trihexyphenidyl, chlorpromazine and clozapine in blood by GC-MS.
Shui-Qing ZHENG ; Wei WANG ; Chen LIANG ; Rong WANG ; Fei-Jun GONG ; Zhong-Ping WU ; Yong-Sheng CHEN ; Yu-Rong ZHANG ; Run-Sheng ZHANG
Journal of Forensic Medicine 2011;27(4):271-273
OBJECTIVE:
To develop a method to measure trihexyphenidyl, chlorpromazine and clozapine in human blood with GC-MS.
METHODS:
The specimens were alkalized (pH > 10) and extracted with V (benzene):V(ethyl acetate) = 1:1, and qualitatively analyzed using GC-MS-Full Scan with internal standard SKF525A. The specimens were alkalized (pH > 10) and extracted with V(benzene):V(ethyl acetate) = 1:1, and quantitatively analyzed using GC-MS-SIM with internal standard diazepam-d5.
RESULTS:
The lowest detection limits of trihexyphenidyl, chlorpromazine and clozapine were 0.3, 0.3 and 0.7 ng/mL (S/N > or = 3) respectively. The calibration curve in 20-10 000 ng/mL showed a good linear distribution. The recovery rate was 79.9% to 85.5%. The RSDs of intraday and interday were less than 5.1%.
CONCLUSION
The established method was simple, sensitive and accurate for simultaneous determination of trihexyphenidyl, chlorpromazine and clozapine in human blood, and can be applied in forensic toxicological cases.
Adult
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Antipsychotic Agents/poisoning*
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Chlorpromazine/blood*
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Clozapine/blood*
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Female
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Forensic Toxicology
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Gas Chromatography-Mass Spectrometry/methods*
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Humans
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Hydrogen-Ion Concentration
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Male
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Middle Aged
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Reproducibility of Results
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Sensitivity and Specificity
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Solvents/chemistry*
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Trihexyphenidyl/blood*
6.The impact of early rapid virological response on the outcomes of hepatitis B associated acute on chronic liver failure during antiviral treatment.
Li CHEN ; Cai-xia ZHENG ; Ming-hua LIN ; Qiao-rong GAN ; Rong-sheng LIN ; Hai-bing GAO ; Jian-rong HUANG ; Chen PAN
Chinese Journal of Hepatology 2011;19(10):734-737
OBJECTIVETo investigate the impact of early rapid virological response on the outcomes of hepatitis B associated acute on chronic liver failure during antiviral treatment.
METHODS106 acute on chronic liver failure patients in our hospital from January 2008 to July 2010 were enrolled in present study retrospectively. Besides internal medicine therapy, all patients received lamivudine (100 mg/d) or entecavir (0.5 mg/d) treatment. The profile of liver biochemistry, prothrombin time activity and viral load were detected at baseline and week 4, respectively. The patients were divided into HBV DNA negative group and HBV DNA positive group according to the viral load at week 4. The clinical features and treatment outcomes were compared between groups. Frequency variables were compared by x2 test or Fisher's exact test. Continuous variables were compared using independent samples T-test. The factors that impact on the treatment outcomes were determined using stepwise multivariate logistic regression analysis.
RESULTSAt the week 4, the TBil and PTA in HBV DNA positive group [(261.6+/-205.6)mumol/L and 44.7%+/-19.7%, respectively] were significantly different from those in HBV DNA negative group [(160.1+/-173.4) mumol/L and 56.8%+/-23.1%, respectively] ( t = 2.190 and -2.077, respectively, P less than 0.05). The non-effective rate of HBVDNA positive group (50%, 9/18) was significantly higher than that of HBV DNA negative group (14.8%, 13/88) (x2 = 9.235, P less than 0.01). By using stepwise multivariate logistic regression analysis, the disease stage and HBV DNA undetectable at week 4 were the independent factor. The OR values of disease stage and HBV DNA undetectable were 6.559 and 0.209, respectively, and 95% CI was 2.316~18.576 and 0.058~0.747, respectively.
CONCLUSIONThe rapid suppression of viral load by nucleotide analogue may improve the efficacy of hepatitis B associated acute on chronic liver failure treatment. The early rapid virological response within first 4 weeks may contribute to the prediction of the treatment outcomes.
Adult ; Antiviral Agents ; therapeutic use ; DNA, Viral ; blood ; End Stage Liver Disease ; drug therapy ; virology ; Female ; Hepatitis B ; drug therapy ; Humans ; Liver Failure, Acute ; drug therapy ; virology ; Male ; Middle Aged ; Prognosis ; Retrospective Studies ; Treatment Outcome ; Viral Load
7.Convolution-based restoration approach to the double fractures of the radius and ulna.
Xiao-Rong ZHENG ; Li-Juan ZHONG ; Qing-Rui XIAO ; Zhen-Sheng WANG ; Ruo-Fan YE ; Xiao-Bao WU
China Journal of Orthopaedics and Traumatology 2012;25(10):875-876
Adolescent
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Adult
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Child
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Child, Preschool
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Female
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Humans
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Male
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Manipulation, Orthopedic
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methods
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Radius Fractures
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therapy
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Ulna Fractures
;
therapy
8.Plaster combined with splint for the treatment of gartland type III humeral supracondylar fractures in children.
Jin-sheng ZHAO ; Guo-zhi CHEN ; Zheng-rong ZHANG ; Wan-qiang TONG
China Journal of Orthopaedics and Traumatology 2011;24(8):672-674
OBJECTIVETo study clinical effects of plaster combined with splint for the treatment of Gartland type 1I humeral fractures.
METHODSFrom March 2002 to May 2006, 24 children with humeral supracondylar fractures of Gartland type ill were reviewed. Among the patients, 14 patients were male and 10 patients were female, ranging in age from 4 to 12 years, averaged 6.6 years. Ten patients had injuries in the left and other 14 patients had injuries in the right limb. Firstly, the patients were treated with manipulative reduction to maintain the length of humerus without emphasis on anatomic reduction. Then, the patients were treated with external fixation using plaster for 5 to 7 days, and secondary manipulative reduction after swelling disappeared. Lastly, the patients were treated with external fixation using splint for 4 to 5 weeks until fracture union.
RESULTSAll the patients were followed up, and the duration ranged from 5 months to 2 years, with an average of 1.2 years. All the patients had no complications such as neurovascular injury, myositis ossificans, forearm compartment syndrome and Volkmann contracture. According to ZHU Xiao-ting evaluation criteria for humeral supracondylar fractures in children, 12 patients got an excellent result, 8 good, 3 poor and 1 bad.
CONCLUSIONTreatment of child humeral supracondylar fractures with plaster and splint has several advantages such as avoiding serious soft tissue injuries around fractures due to many times reduction, fracture dynamic correction to obtain satisfactory reduction, reducing complications, obtaining good reduction, and restoring elbow function in a relatively short period of time.
Casts, Surgical ; Child ; Child, Preschool ; Female ; Humans ; Humeral Fractures ; diagnostic imaging ; surgery ; Male ; Radiography ; Splints
9.Value of serum-vascular endothelial growth factor in the differential diagnosis of solitary pulmonary nodule.
Zheng-liang TU ; Guo-wei YU ; Zhong-rong HU ; Yi-ming NI ; Ding-sheng YE
Chinese Journal of Oncology 2003;25(2):154-156
OBJECTIVETo evaluate serum-vascular endothelial growth factor (S-VEGF) in the differentiation of solitary pulmonary nodule (SPN).
METHODSSerum level of VEGF of 68 patients with SPN was measured by ELISA kit, and compared with the control group of 20 normal subjects. The nodules were diagnosed by operation and pathology.
RESULTSThe median level of S-VEGF was 42.5 (range from 10 to 170) pg/ml in the control, 44 (range from 18 to 360) pg/ml in benign nodule group and 75 (range from 18 to 890) pg/ml in lung cancer group, with significant difference observed between the nodule group and control (P < 0.01), and between the lung cancer group and the benign nodule group (P < 0.05), but not between the benign nodule group and the control. In addition, when S-VEGF in different pathologic types of the limited number of lung cancer patients were compared, no significant difference was observed.
CONCLUSIONS-VEGF is valuable in the differential diagnosis of solitary pulmonary nodule. An elevated S-VEGF level >or= 100 pg/ml in patients with SPN may strongly speak for a malignant nodule. Operation is suggested.
Adult ; Aged ; Diagnosis, Differential ; Female ; Humans ; Lung Neoplasms ; blood ; diagnosis ; Male ; Middle Aged ; Solitary Pulmonary Nodule ; blood ; blood supply ; diagnosis ; Vascular Endothelial Growth Factor A ; blood
10.CT evaluation of lymphoma in the abdominal lymph nodes.
Ri-sheng YU ; Shi-zheng ZHANG ; Jin-xiu WU ; Rong-fen LI
Journal of Zhejiang University. Medical sciences 2004;33(3):269-276
Adolescent
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Adult
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Aged
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Diagnosis, Differential
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Female
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Humans
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Lymph Nodes
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diagnostic imaging
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Lymphoma
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diagnostic imaging
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pathology
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therapy
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Male
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Middle Aged
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Tomography, X-Ray Computed