Adult ; Histiocytosis, Langerhans-Cell ; Humans ; Male

Poly(U)-binding-splicing factor 60 KDa (PUF60), a U2-related splicing factor that facilitates 3′splice-site recognition at the early stage of spliceosome assembly.High expression of PUF60 is related to the occurrence of multiple tumors, such as colorectal cancer, ovarian cancer, gastric cancer, liver cancer, et al.PUF60 can regulate the expression of c-myc through the core-human transcription factor Ⅱ basal transcription factor.Anti-PUF60 antibodies are detected in the sera of patients with early-stage and recurrent tumor, and the levels are significantly decreased after the operation.PUF60 can be used as a combined or independent test index for the diagnosis of cancer, which can be a potential new target for gene therapy.

Objective To observe the effect of Maiya on the hormone level of the female mice. Methods Female mature mice (5 ～ 6 months) were ovariectomized and treated with Maiya. Follicular stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2) and progesterone (P) in blood were examinated. Results Maiya could increase the FSH and E2 in blood, and decrease P in blood of the castrated mice, while the level of LH had no obvious change. Conclusion Maiya can influence the level of hormone.

Objective To explore the clinical significance of hydrogen sulfide(H2S)and heme oxygenase-1(HO-1)on pulmonary hypertension(PH),by detecting their levels of patients with congenital heart disease(CHD).Methods Forty-eight patients with CHD who had left-to-right shunt were selected randomly from cardiac surgery in the Fourth Hospital of Hebei Medical University.Forty-eight patients were performed successfully on radical operation under complex intravenous anesthesia,and they were divided 3 groups according to pulmonary artery systolic pressure(PASP)detected by doppler echocardiography before operation:no PH group(PASP0.05).Conclusions H2S plays an important role in the formation of PH and restructure.There is no correlation between PASP and HO-1 activities in serum,but HO-1 may be play an indirect action in the formation of PH and restructure.Measuring the levels of H2S may be a reliable method to follow the change of pulmonary pressure and worsening of PH.

OBJECTIVE:To establish the HPLC method for the content determination of paeoniflorin and liquoritin in Jiawei xiaoyao pills. METHODS:It was performed on Hypersil-ODS C18 with the mobile phase of acetonitrile-water(14∶86,V/V)at the flow rate of 1.0 ml/min,the detection wavelength was 230 nm,the column temperature was room temperature and the volume was 10 μl. RESULTS:The linear range was 16.12-80.6 μg/ml for paeoniflorin(r=0.999 6)and 6.08-30.4 μg/ml for liquoritin(r=0.999 8);the RSDs of precision,repeatability and stability tests were all less than 2.0%;the average recovery was respectively 98.51%(RSD=1.94%,n=6)and 98.08%(RSD=1.29%,n=6). CONCLUSIONS:The method is simple,accurate and reliable, and can be used for the content determination of paeoniflorin and liquoritin in Jiawei xiaoyao pills.

0 05).Conclusion:Intraoperative lymphatic mapping with methylene blue is easily performed in early breast cancer patients.To determine the sentinel lymph node and status of axillary lymph node metastasis by pathological examination of the stained lymph nodes,there was hight false negative rate and unacceptable rate of misdiagnosis.So,its feasibility need further evaluation.[

Objective:To provide some suggestions for improving the pharmaceutical advertisement supervision system and standardizing the pharmaceutical advertisement market. Methods: Literature reviews, statistical analysis and interviews were adopted to study the status quo and difficulties of pharmaceutical advertisement supervision and pharmaceutical advertisement trends. Results:The top-level design of pharmaceutical advertisements supervision sys-tem has two defects:the separation between approval and penalty and the obstacles to cooperation at all levels of the drug administration. The worsening status quo of illegal drug advertising has elicited concern from scholars and regu-lators. However, there are still some difficulties in the supervision of drug advertisers, advertising agents, and pub-lishers. Conclusion:It is suggested that the drug advertising regulation system should be perfected by strengthening information supervision, establishing a coordination mechanism and consolidating self-discipline consciousness. Addi-tionally, laws and regulations should be promptly revised as an essential way to improve the effects of supervision.

Objective To investigate the nutritional status and glycemic control level in elderly patients with type 2 diabetes in urban community of Yangpu district in Shanghai.Methods The 24-hour dietary recall method for 3 consecutive days was used to investigate the dietary nutrients intake in 400 patients with type 2 diabetes.Results The ratio of patients with blood glucose level less than 6.1mmol/L was 23.3％ (93/400).The energy intake in patients with type 2 diabetes was low,and only reached 62.81％ of the recommended standard of energy intake for adults.The energy ratio of three diet nutrients (protein,fat and carbohydrate) were 14.41％,33.93％,51.66％ respectively,which showed that the energy ratio of fat was relatively high.The energy ratio of breakfast,lunch and supper dinner were 23.37％,38.05％ and 38.57％ respectively.The average intake of vitamin E,iron,niacin,manganese,phosphorus and cholesterol in elderly patients was more than the recommended standard of adult diet nutrients,and reached 149.6％,138.1％,137.1％,132.5％,127.5％ and 122.0％ of the recommended standard respectively.But the average intakes of protein,fat,carbohydrate,dietary fiber,calcium,zinc,copper,magnesium,potassium,vitamin A,vitamin B1,vitamin B2 and vitamin C in elderly patients were lower than the recommended standard,and reached 77.9％,90.3％,57.1％,50.7％,49.3％,81.4％,75.7％,75.7％,77.5％,77.0％,55.1％,58.1％ and 67.6％ of the recommended standard respectively.Conclusions The status of dietary nutrients intake in elderly patients with type 2 diabetes in Yangpu district of Shanghai is unreasonable,the intakes of dietary fiber,calcium,vitamin B1,vitamin B2 etc.were insufficient,and reasonable diet instruction should be provided for the elderly patients.

Breast cancer stem cells (BCSC) are group of cells exhibiting self-renewal and multi-directional differentiation potentials. These cells have an important role in the occurrence, development, metastasis, and recurrence of breast cancer. In normal circumstances, the ability of mammary stem cells to differentiate and undergo self-renewal is governed by related signaling pathways. After this mechanism is destroyed, breast stem cells undergo abnormal differentiation, forming breast cancer stem cells that unlimitedly proliferate to develop into breast cancer. As research on BCSC increasingly deepens, regulation of BCSC by notch signaling and its crosstalk with several signaling pathways have drawn a great deal of attention in this field. This paper reports the signaling pathways of breast cancer stem cells and latest studies on this field to better understand the essential role of notch signaling pathway in the occurrence and development of breast cancer and corresponding clinical targeted therapy.

The presence and persistence of systemic and lung inflammation in children with acute respiratory distress syndrome (ARDS) is the basis for the use of corticosteroids as a therapeutic agent.The trial of ARDS treated with high-dose short-course corticosteroids showed no benefit,even increase motality.At present,the results of randomized controlled trial and meta-analysis suggested that low-dose and replacement-dose methylprednisolone [1-2 mg/(kg· d)] or equivalent types of corticosteroids may decrease the fatality rate,reduce staying days in ICU and the duration of mechanical ventilation.Use of corticosteroids for ARDS in children is still lack of multicenter randomized controlled trial.