OBJECTIVE:To establish an RP-HPLC method for the determination of Berberine hydrochloride in Keyang-min tincture.METHODS:The sample separation was performed on Agilent Eclipse XDB C18(250 mm?4.6 mm,5 ?m),and the mobile phase was acetonitrile-5 mmol?L-1 perfluorooctane sulfonate(32∶68) at flow rate of 1 mL?min-1.The detection wavelength was 346 nm and the temperature of column was 35 ℃.RESULTS:The linear range of Berberine hydrochloride was 0.059 2～0.888 3 ?g(r=0.999 9).The average recovery rate was 99.71%(RSD=1.18%,n=6).CONCLUSION:The met-hod is sensitive,accurate,and reproducible,and suitable for the determination of Berberine hydrochloride in Keyangmin tincture.

OBJECTIVE:To study the effect of Hordeum vulgare on hormone level in male mice.METHODS:Male mature mice(5～6 months) were treated with Hordeum vulgare decoction 1 mL(equivalent to 0.5 g?mL-1 crude drug) bid for 14 consecutive days.Follicle-stimulating hormone(FSH),luteinizing hormone(LH),estradiol(E2) and testosterone(T) in blood were monitored.RESULTS:In Hordeum vulgare group compared normal control,serum E2 increased significantly,serum level of testosterone decreased significantly,while FSH and LH levels remained stable.CONCLUSION:Hordeum vulgare can influence the hormone level in male mice by influencing the gonad axis of the mice.

0.05); the effective rates in rest pain in the two groups were 41.25% and 57.97%,respectively(P

Objective In order to explore the effective dosage of insulin and its correlated elements in sulphonylureas failure in type 2 diabetes mellitus.Methods 61 cases of type 2 diabetics were separated into two groups,one group (group 1) is the first time using insulin and the other group (group 2) is in the use of insulin in the past.Results The effective insulin dosage of group 2 is significantly higher than that of group 1 (36.2?10.6 IU/d vs 30.1?10.5IU/d, P

Objective To observe the effect of Maiya on the hormone level of the female mice. Methods Female mature mice (5 ～ 6 months) were ovariectomized and treated with Maiya. Follicular stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2) and progesterone (P) in blood were examinated. Results Maiya could increase the FSH and E2 in blood, and decrease P in blood of the castrated mice, while the level of LH had no obvious change. Conclusion Maiya can influence the level of hormone.

This paper analyzes the reform backgrounds, mechanisms and measures of financing and payment taken in the second-generation national health insurance (NHI) in Taiwan. The results indicate that in 2nd NHI, the rate was adjusted more flexibly, supplementary insurance premium was charged to enlarge the financial source by im-plicating additional subsidies and expanding the sources of financing, multiple payment methods and auxiliary assis-tive means were used to control the growth of medical expenses, social insurance payments are specified in terms of category or clear payment projects and standards, new health technology assessments are used as new basis for deci-sion making, etc. by greatly alleviating the financial deficit, which helped achieve the financial balance again. Many features of the 2nd NHI in Taiwan, especially like its diversified financial resources and financing methods, global budget of control fees and DRGs payment systems, making evaluation criteria for medical quality control and health insurance reimbursement project development and so on, are worth learning for the mainland China.

Objective To find out the status of nosocomial infection management in hemodialysis sections, in order to encourage their continuous quality improvement and standardize their management Methods 41 hemodialysis sections with (≥) 3 dialyzers and independent operation were chosen for investigation. On-site investigation and environmental microbiology monitoring combined. Results The higher the level of the hospital, the better the nosocomial infection control in its implementation of the infection control measures. In terms of rules and regulations, incompliant secondary hospitals and private hospitals accounted for 56. 2% of hospitals of the same ranking; in terms of layout and processes, 75% are found incompliant; and various defects are found in the dialyzer reuse management and disinfection in tertiary and lower hospitals. As to hand hygiene of the staff, the incompliance rate is over 55%. As indicated in the results of microbial environment monitoring, the compliance rate of dialysis water is over 90%, that of dialysate is 78.6% ～ 86.2%, and that of hand disinfection is 56.6%～78.6%.Conclusion It is imperative to strengthen the supervision of secondary hospitals and private hospitals,and to train their staff in the knowledge of hand hygiene and nosocomial infection in hemodialysis rooms.Hospitals found with poor infection management will be encouraged to take issue-specific actions for rectification.

Objective: To probe into the relationship between changes of sex hormone levels and TCM syndrome differentiation in climacteric women.Methods: According to TCM syndrome differentiation,55 cases of women in menopause were divided into three groups: kidney yang deficiency,kidney yin deficiency and the normal;the correlation between the three types and E2,FSH and LH was explored by statistical methods,and then they were compared with procreative women.Results: In all the 55 cases,kidney yin deficiency covered 70.90%,kidney yang deficiency covered 16.36%,the normal covered 12.72%;changes of E2 correlated positively with symptom scores of kidney yang deficiency and kidney yin deficiency;changes of FSH and LH correlate negatively with that of kidney yang deficiency and kidney yin deficiency.And they all correlated closely with menopause.Conclusion: the main syndrome type of climacteric women was kidney yin deficiency;E2↓,FSH↑ and LH↑ was expression of physiological kidney deficiency of climacteric women.

Objective To study the influence of pine-seed oil upon body weight,blood lipid and lipid peroxidation of mice fed on high fat feed.Method The mice were fed by high fat feed(80% basal feed added 20% lard,HF) and HF added pine-seed oil(80% high fat feed added 20% pine-seed oil,SHF).At 45 d and 90 d,the body fat content,blood lipid level,and malonyldehyde(MDA),lipofusin(LP),catalase(CAT),superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) of brain and liver homogenate were measured respectively.Results Pine-seed oil could reduce the body weight serum triglyceride(TG),total cholesterol(TC),low density lipoprotein-cholesterol(LDL-C) content,but increase high density lipoprotein-cholesterol(HDL-C) of the mice fed by HF.It suggested that pine-seed oil had definite effect on regulating blood lipid.Compared with normal control(NC) group,the activities of CAT、T-SOD、GSH-Px of brain and liver in HF group were remarkably lower,but MDA and LP remarkably higher.It suggested that HF could promote lipid peroxidation in brain and liver of mice.Compared with HF group,the activites of CAT,T-SOD and GSH-Px of brain and liver in SHF group were remarkably higher,but MDA remarkably lower.Conclusion Pine-seed oil can reduce the body weight,blood lipid,and lipid peroxidation of mice fed by HF.

Objective]Observe the clinical effect of early herpes zoster which were treated by venesection ventouse combined with helium-neon laser and drugs. [Methods]The control group 32 cases used Famciclovir Tablet,transfer factor capsules,mecobalamin and acyclovir cream-drug for external use. While the experimental group 32 cases used Famciclovir Tablet,transfer factor capsules,mecobalamin and acyclovir cream-drug for external use. While the experimental group besides taking above-medicine, were treated with venesection ventouse and helium-neon laser at the pathological change. [Results]The control group used to cure for an average of 13 ±2.5 days. The experimental group used to cure for an average of 9.6 ±2.4 days. There were significant differences(P<0.05). [Conclusion] Early herpes zoster treated by venesection ventouse combined with helium-neon laser and drugs, can significantly shorten the duration of symptoms, alleviate suffering, and has good clinical efficacy.