OBJECTIVE:To establish an RP-HPLC method for the determination of Berberine hydrochloride in Keyang-min tincture.METHODS:The sample separation was performed on Agilent Eclipse XDB C18(250 mm?4.6 mm,5 ?m),and the mobile phase was acetonitrile-5 mmol?L-1 perfluorooctane sulfonate(32∶68) at flow rate of 1 mL?min-1.The detection wavelength was 346 nm and the temperature of column was 35 ℃.RESULTS:The linear range of Berberine hydrochloride was 0.059 2～0.888 3 ?g(r=0.999 9).The average recovery rate was 99.71%(RSD=1.18%,n=6).CONCLUSION:The met-hod is sensitive,accurate,and reproducible,and suitable for the determination of Berberine hydrochloride in Keyangmin tincture.

OBJECTIVE:To study the effect of Hordeum vulgare on hormone level in male mice.METHODS:Male mature mice(5～6 months) were treated with Hordeum vulgare decoction 1 mL(equivalent to 0.5 g?mL-1 crude drug) bid for 14 consecutive days.Follicle-stimulating hormone(FSH),luteinizing hormone(LH),estradiol(E2) and testosterone(T) in blood were monitored.RESULTS:In Hordeum vulgare group compared normal control,serum E2 increased significantly,serum level of testosterone decreased significantly,while FSH and LH levels remained stable.CONCLUSION:Hordeum vulgare can influence the hormone level in male mice by influencing the gonad axis of the mice.

0.05); the effective rates in rest pain in the two groups were 41.25% and 57.97%,respectively(P

This paper analyzes the reform backgrounds, mechanisms and measures of financing and payment taken in the second-generation national health insurance (NHI) in Taiwan. The results indicate that in 2nd NHI, the rate was adjusted more flexibly, supplementary insurance premium was charged to enlarge the financial source by im-plicating additional subsidies and expanding the sources of financing, multiple payment methods and auxiliary assis-tive means were used to control the growth of medical expenses, social insurance payments are specified in terms of category or clear payment projects and standards, new health technology assessments are used as new basis for deci-sion making, etc. by greatly alleviating the financial deficit, which helped achieve the financial balance again. Many features of the 2nd NHI in Taiwan, especially like its diversified financial resources and financing methods, global budget of control fees and DRGs payment systems, making evaluation criteria for medical quality control and health insurance reimbursement project development and so on, are worth learning for the mainland China.

Objective To observe the effect of Maiya on the hormone level of the female mice. Methods Female mature mice (5 ～ 6 months) were ovariectomized and treated with Maiya. Follicular stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2) and progesterone (P) in blood were examinated. Results Maiya could increase the FSH and E2 in blood, and decrease P in blood of the castrated mice, while the level of LH had no obvious change. Conclusion Maiya can influence the level of hormone.

Objective In order to explore the effective dosage of insulin and its correlated elements in sulphonylureas failure in type 2 diabetes mellitus.Methods 61 cases of type 2 diabetics were separated into two groups,one group (group 1) is the first time using insulin and the other group (group 2) is in the use of insulin in the past.Results The effective insulin dosage of group 2 is significantly higher than that of group 1 (36.2?10.6 IU/d vs 30.1?10.5IU/d, P

Literatures available was reviewed on the thrombosis and mechanism of new antithrombotic agents.The pathophysiology of the thrombosis involves dysfunction of endothelium and cell adhesion.The new antithrombotic drugs include factor Ⅹa inhibitors, GPⅡb/Ⅲa receptor blocking agents and the drugs modulating the function of endothelium.

Purpose To study the spiral CT (SCT) appearances of pleural plaques,to improve the accuracy of diagnosis and differential diagnosis,and to evaluate clinic application.Materials and Methods The SCT appearances in 42 cases with pleural plaques were analysed retrospectively,which closed met with asbestos dust.Results SCT showed 407 pleural plaques in 42 cases,of which there were hyline / uncalcified pleural plaques (n=250),calcified pleural plaques(n= 36),mixed pleural plaques(n=121);unilateral pleural plaques (n=10),bilateral pleural plaques (n=32);single pleural plaque in 5 cases,multiple pleural plaques in 37 cases.The largest pleural plaque was 4.2cm×1.2cm.There were a lot of shapes of the pleural plaques.Various shapes of calcifications could be showed in the calcified or mixed type pleural plaques.The pleural plaques distributed in the middle-inferior part,anterior / posterior-lateral chest wall,underlying the ribs in 28 cases (66.6 %),both bilateral domes of diaphragm in 10 cases and mediastinal pericardium in 4 cases.The pleural plaques associated with pulmonary intertitial fibrosis in 4 cases.No cases in the apices / costophrenic angles.No enhancement of pleural plaques in 6 cases which were administrated contrast scan.Conclusion SCT can clearly show the distribution,extention,shape,size,density and relation to adjacent tissues of the pleural plaques,and with asbestos exposure history,can make a diagnosis and differential diagnosis from other pleural thickened lesions.SCT is the best choice of imaging methods for asbestosis and pleural plaque.

Acid-related disorders are commonly seen diseases of upper digestive tract which are closely related to acid attack.Antacids and acid inhibitors are the main drugs in the therapy of acid-related disorders.There are complex interrelationships between antacids and acid inhibitors and other drugs.This article reviewed the application and evaluation of antacids and acid inhibitors in acid-related disorders.

Objective To explore the C-type fixation in the application of the catheter tunnel peritoneal dialysis. Methods One hundred and twenty patients undergoing peritoneal dialysis were selected from January to December 2014 in Xijin Hospital and were randomly divided into the control group and experiment group. The peritoneal dialysis catheters of the control group were fixed vertically at the tube orifice with surgical tape, while the peritoneal dialysis catheters of the experiment group were fixed horizontally first and then placed with a shape ofC and fixed at the tube orifice with surgical tape. The two groups were compared in terms of tunnel score, infection, tube shedding and pulling-induced stimuli. Result The tunnel score and the cases of infection rate, tube shedding and pulling-induced stimuli in the experiment group were lower and fewer than those of the control group with statistical significance (all P <0.05). Conclusion The type C fixation of tube for peritoneal dialysis can effectively reduce the incidence of infections in patients with peritoneal dialysis tunnel, reduce pulling-induced stimuli, and improve comfort of the patients.