Community health-care service is a dynamic and comprehensive primary health care for a given population.The increasing health-care information needs to be dynamically managed and fully utilized.The comprehensive and networking management of the medical care information with computer in community is scientific and effective in community health care service.Accordingly,The working principle of network computer information management system for community health-care service is made and the regarded functional models are established.The data in the system is convenient for work summary and scientific research.The work protocol based on the system can help realize the standardized management to community health-care service.

Objective To study the AmpC beta-lactarnase and its genotype mediated by plasmid in Escherichia coli.Methods AmpC beta-lactamase was detected based on that AmpC beta-laetamase can be inhibited by 3-aminophenylboronic acid(APB).MIC was ehecked by agar dilution method.Conjugation test was used to check the transfer of ampC gene.Gene chip and PCR were used to detect ampC gene.The amplified ampC gene were sequenced and analyzed by EMBOSS software.The molecular epidemiology of clinical isolates was investigated by Enterbacterial repetitive intergenic consensus(ERIC)typing method.Results In 74 strains of Escherichia coli insusceptible to cefoxtin,AmpC beta-lactamase was positive in 33 strains.8 strains possessed AmpC beta-lactamase of CIT group by gene chip and 8 transconjugants were obtained by conjugation test.CMY type ampC gene could be further amplified by specific CMY gene primers from both 8 clinical isolates of E.coli and plasmids extracted from 8 transconjugants.CMY-2 type ampC gene was found by sequencing(accession number DQ823449).The most transconjugants displayed similar MIC value(intermediate or resistant).ERIC genotyping showed 6 out of 8 isolates with CMY-2 ampC gene derived from different resource.Conclusion CMY-2 AmpC beta- lactamase mediated by plasmid could be detected in E.coli isolates from patients in the General Hospital of People Liberation Army,Beijing.The plasmid carried ampC gene could mediate multi-drug resistance.

Objective To investigate the causes and therapeutic methods of compartment syndrome following radial artery cannulation in the left forearm.Methods The data(including puncture pin type,puncture frequency, onset time and INR) and therapeutic methods(including using manicol,cold compress,fasciotomy,dactylolysis and dermatoplasty) of 6 patients were retrospectively analyzed,which had compartment syndrome after radial artery can- nulation in the left forearm.Results The type of puncture pin and puncture frequency attributed to this disease to some extent.After therapy,4 patients recovered completely.2 patients underwent fasciotomy,and Volkmann syn- drome happened in 1 patient,dactylolysis happened in the other because of necrosis in the left thumb and finger.Con- clusion Compartment syndrome may be avoided by correct selection of puncture pin type and no repeated punctur- ing,and early diagnosis and therapy are important for fine prognosis.

Objective To investigate the effect induced by specific inducible nitric oxide synthase (iNOS) inhibitor 1 400 W on nasopharyngeal carcinoma CNE-2 cells lines and its mechanism.Methods CNE-2 cells were treated by different concentrations of 1 400 W,diamminedichloroplatinum (DDP),and both chemicals.Cell counting kit-8 (CCK-8) was used to examine the viability of cells.Quantitative realtime polymerase chain reaction (qRT-PCR) was applied to detect the iNOS mRNA expression.Results The expression of iNOS mRNA was down-regulated by 1400W and was positively correlated with inhibitionrate of cell proliferation.1 400 W inhibits proliferation of CNE-2 cell in a concentration-dependent manner.The proliferation inhibition rate of CNE-2 cells treated by 1 400 W combined with DDP was not enhanced.Conclusions Specific inducible nitric oxide synthase inhibitor 1 400 W can exerts anti-tumor effect though inhibiting the expression of iNOS mRNA;The mechanism of chemosensitization induced by iNOS inhibitor on CNE-2 cells may be closely related level of down-regulation of iNOS expression.

Objecfive To investigate antibiotic resistance,carbapenemase genotype and the molecular epidemiology of multidrug-resistant Acinetobacter baumannii (Aba) collected from 3 military hospitals in China.Methotis The minimum inhibitory concentrations (MIC) were examined by ager dilution method.Genotypes of carbapenemases were amplified by multiplex PCR and its products were sequenced.PCR was used to detect per gene,Homology of the resistant isolates was analyzed by pulse-field gel electrophoresis(PFGE).Results Among the 64 MDRA strains,78.1%(50)strains possessed blaOXA-23 gene,89.1%(57) carried Class 1 integrase gene,39.1% (25) with blaPER-1 gene,and 1 strain with blaOXA-58-like gene.PFGE showed that 13(A,B,C,D,E genotype) different clones were identified in these strains.A,B,and U clones were the predominant clones in three hospitals,respectitively.Conclusion OutbreaEs of muitidrug-rcsistant Aba occurred at 3 military hospitals with the most prevalent carbapenemase as OXA-23 enzyme.OXA-58 type of carbapenemase and per-1 in Aba were also isolated.

Objective: To conduct a comparative study on the specific effects of systematic sex education on adolescent students in terms of sexual cognition, sexual values, and sexual adaptation, and to provide the support for conducting a comprehensive education in middle schools. Methods: A whole group sampling method was used to select 3 369 middle and high school students from six general and vocational middle schools in Sichuan Province, which were divided into systematic sex education schools and non systematic sex education schools, and a comparative study was conducted using the Adolescent Mental Health Scale. Results: The results showed that the adolescent students who received systematic sex education were significantly different from those who did not receive systematic sex education in terms of sex related cognition (7.18±6.24, 5.65±7.40), sexual values(7.60±1.17，7.30±1.24), and sexual adjustment (11.49±1.29，11.10±1.41). All differed significantly ( t =5.95, 6.80,7.57, P <0.01). The students who received systematic education in junior middle school were higher than those who received non systematic education in sex related cognition, sexual values and sexual adaptation ( P <0.01). However, in senior high school, the differences in systematic education are only shown in sexual values control and self adaptation in sexual adjustment ( P <0.01). There were significant differences in sex related cognition, sexual values and sexual adjustment between male and female students who received systematic education and non systematic education ( P <0.01). Conclusion Systematic sex education is more beneficial to the psychosexual health of adolescent students than non systematic sex education in schools.

OBJECTIVETo explore the mechanism of cake-separated moxibustion in treatment of hyperlipemia.

METHODSSixty rabbits were randomly divided into 4 groups, a blank group,a model group, a direct moxibustion group and a cake-separated moxibustion group. Hyperlipemia model was developed by high fat diet of cholesterol. Changes of ultrastructures of endothelial cells of the aorta of the rabbit were observed with electron microscope.

RESULTSThe endothelial cells in the cake-separated moxibustion group were more intact, most of them were normal in forms, internal elastic membrane was continuous, their thickness was even, the cells of smooth muscles in the medial membrane were relatively normal, which are similar to those in the blank control group. But the structure of endothelial cells of the aorta in the model group disappeared, in cytoplasm the sedimentation of a great number of lipids can be seen, internal elastic membrane was interrupted, the thickness was uneven, with focal dissolution, the cells of smooth muscle in the medial membrane had sedimentation of lipids, with frothy change.

CONCLUSIONCake-separated moxibustion has a certain protective action on endothelial cells of the aorta in the rabbit of hyperlipemia.

Animals ; Aorta ; Endothelial Cells ; Hyperlipidemias ; Lipids ; Moxibustion ; Rabbits

OBJECTIVETo study the effect of Trichinella spiralis (T.spiralis) infection on the expression and distribution of colonic epithelial E-cadherin in mice and its mechanism.

METHODSBALB/c mice and STAT6-/- mice were infected with T.spiralis, and mice without infection were used as control. Seven days later, the horseradish peroxidase (HRP) was infused by rectal enema. Serum HRP was detected in the subsequent 0, 60 and 120 minutes. Then the mice were sacrificed and colon was taken out. The distribution of E-cadherin in colon was detected by immunofluorescence staining, and the expression of E-cadherin was detected by Western blot. The expression of interleukin-4 (IL-4) in mesenteric lymph nodes was detected by ELISA.

RESULTSSerum HRP level in infected BALB/c mice was significantly higher than that in control mice (P<0.05), while it was not significantly different between infected STAT6-/- mice and controls (P>0.05). In infected BALB/c mice, E-cadherin located in cytoplasm of colonic epithelial cells, while in controls, it located in cellular membrane. E-cadherin expression down-regulated significantly in infected BALB/c mice as compared to controls. E-cadherin expression and distribution did not change obviously in infected STAT6-/- and control mice. IL-4 level in mesenteric lymph nodes of infected BALB/c mice [(193.0±12.5) μg/L] was significantly higher as compared to control BALB/c and infected STAT6-/- mice [(21.0±2.3) μg/L and (15.0±3.1) μg/L, all P<0.05].

CONCLUSIONT.spiralis infection can increase colonic epithelial permeability of mice, which may be associated with induction of Th2 cytokine secretion.

Animals ; Cadherins ; metabolism ; Colon ; metabolism ; Disease Models, Animal ; Female ; Interleukin-4 ; metabolism ; Intestinal Diseases, Parasitic ; metabolism ; Intestinal Mucosa ; metabolism ; Lymph Nodes ; metabolism ; Mice ; Mice, Inbred BALB C ; Mice, Knockout ; Trichinella spiralis ; Trichinellosis ; metabolism

OBJECTIVETo measure anatomic data of laminar of C7 and evaluate the possibility of the placement of C7 laminar screw.

METHODSTwenty-two specimens of C7 were measured with an vernier caliper and a goniomete and finded out the anatomic data for prominent vertebra laminar screw placement. The indicators included the thickness of the superior, median and inferior part of C7 laminar (T1, T2, T3), the height of the laminar(H), the distance of the anchor point to the medial wall of C7 pedicle and the lateral wall of the lateral mass (L1, L2) and the angle between the axis of C7 lamina and sagittal plane(alpha).

RESULTSThe mean thickness of superior part of C7 lamina (T1) was (4.30 +/- 0.27) mm, (5.32 +/- 0.27) mm in median part (T2) with 27% (6 cases) of specimens thickness less than 4.50 mm and (4.78 +/- 0.26) mm in inferior part (T3). Both median part and inferior part were thicker than 4.50 mm. The mean height of C7 laminar (H) was (14.83 +/- 0.26) mm. The distance from the laminar anchor point to the medial wall of C7 pedicle (L1) and the lateral wall of the lateral mass (L2) was (26.99 +/- 0.48) mm and (41.60 +/- 1.10) mm respectively. The mean angle between the axis of C7 laminar and sagittal plane(alpha) was (49.3 +/- 1.1) degrees.

CONCLUSIONIt is feasible to place screw in C7 laminar and this technique can be applied in 73% of Chinese. Compared with C7 pedicle screw technique, this technique is simple with little risk to the nerve and vascular structures.

Adult ; Bone Screws ; Feasibility Studies ; Humans ; Internal Fixators ; Spine ; anatomy & histology ; surgery

OBJECTIVETo observe the clinical efficacy and the change of airway responsiveness to Chinese medicine (CM) in treating cough variant asthma (CVA).

METHODSNinety-four patients who had confirmed the diagnosis of CVA were selected and randomly assigned to the treatment group and the control group by the blocked randomization method. The ratio of the two groups was 2:1. The treatment group had 63 patients that were treated by CM, lost in 10 cases, 53 patients had finished the trial. The control group had 31 patients that were treated by montelukast tablets and theophylline, lost in 5 cases, 26 patients had finished the trial, two weeks as one therapeutic course. The syndrome efficacy, cough efficacy, symptom score and the airway responsiveness between two groups were observed.

RESULTSThe comparison of the syndrome efficacy: the total effective rate of the treatment group was 90.57% and the control group was 76.92%, and the two groups were significantly different (P<0.05). The comparison of the cough efficacy: the total effective rate of the treatment group was 98.11% and the control group was 80.77%, and the two groups were also significantly different (P<0.05). Syndrome scoring and cough scoring were all significantly lowered, but the airway responsiveness was not significantly lowered.

CONCLUSIONSThe treatment of CM could ease the cough, improve the syndrome, and shows obvious advantages compared with the control group, which is worthy of extensive clinical application.

Adult ; Cough ; drug therapy ; Drugs, Chinese Herbal ; adverse effects ; therapeutic use ; Female ; Humans ; Male ; Respiratory System ; pathology ; Syndrome ; Treatment Outcome