AIM: To investigate the effect of mydriasis optometry combined with amblyopia treatment on refraction and amblyopia changes in children with mixed astigmatism and amblyopia.METHODS: Totally 163 children (289 eyes) of mixed astigmatism and amblyopia from January 2010 to May 2011 were treated.All of the patients received mydriatic optometry and spectaculars with amblyopia therapy and were followed up for 5a to observe amblyopia efficacy and refractive status changes.RESULTS: With 5a, main diameter diopter at distant vision decreased year by year, average decline in the first year was 0.55DS, 0.56DS in the second year, 0.72DS in the third year, 0.95DS in the fourth year, 1.89DS in the fifth year.The spherical equivalent changed from 1.12DS at distant to 0.78DS at near.The corrected visual acuity of all the patients at first visit was 0.2-0.8 with varying degrees amblyopia.After a 5-year treatment, it was effective in 268 eyes (92.7%), in which 165 eyes (57.1%) improved, 103 eyes (35.6%) cured, the results was better as the period of spectaculars wearing was longer.There was 36 eyes (37.5%) improved and 60 eyes (62.5%) cured in 1.50-2.50DC group;118 eyes (74.2%) improved, 41 eyes (25.8%) cured in 2.50-3.50DC group;11 eyes (32.3%) improved, 2 eyes (5.9%) cured, 21 eyes (61.8%) useless in >3.50DC Group.The differences of efficacy among the groups were significant (all P<0.05).CONCLUSION: Appropriate spectaculars is the basic for amblyopia treatment.It is effective for most children with mixed astigmatism and amblyopia to take mydriasis optometry and amblyopia treatment.

AIM: To analyze the pathogens and drug sensitivity of chronic dacryocystitis in order to provide evidence for clinical drug use.
METHODS:Lacrimal secretion of 171 cases with chronic dacryocystitis was sampled for pathogenic bacteria culture identification and drug sensitivity test. Based on the results, the isolation rate of pathogens strains, the pathogens kind of chronic dacryoeystitis, main pathogens of chronic dacryocystitis, and sensitive drug for pathogens were analyzed.
RESULTS: The isolation rate of pathogens strains was 76. 61% ( 131 cases ). The pathogens constituting the chronic dacryocystitis were predominantly gram-positive coccus,the percentage was 72. 52% (95 cases), among which staphylococcus hominis occupied 27. 48% ( 36 cases), staphylococcus epidermidis 16. 79% (22 cases), streptococcus viridans 12. 98% (17 cases). The majority of these bacteria were sensitive to cefoperazone-sulbactam, tobramycin, gentamicin and levofloxacin. For gram -positive coccus, cefoperazone - sulbactam, gentamicin and tobramycin were the most sensitive drug. For gram-negative bacilli, cefoperazone - sulbactam, tobramycin and levofloxacin were most sensitive drug.
CONCLUSION: Staphylococcus hominis is the main pathogen of chronic dacryocystitis, tobramycin can be used as the first choice for local treatment of chronic dacryocystitis.

Adenoma, Sweat Gland ; pathology ; Humans ; Male ; Middle Aged ; Nose Neoplasms ; pathology

Diagnostic Errors ; Electrocardiography ; Humans ; Inferior Wall Myocardial Infarction ; Meningeal Neoplasms ; Meningioma ; Myocardial Infarction ; diagnosis

Adult ; Dimethylformamide ; analysis ; toxicity ; Dose-Response Relationship, Drug ; Humans ; Liver ; drug effects ; Male ; Middle Aged ; Occupational Exposure ; statistics & numerical data ; Physical Examination ; statistics & numerical data

Adolescent ; Adult ; Female ; Humans ; Male ; Occupational Exposure ; Organotin Compounds ; poisoning ; Poisoning ; diagnosis ; therapy ; Retrospective Studies ; Young Adult

Objective To know the relationship between the uterine fibroid symptom severity and the quality of life andthe correlation among all the subscales of the quality of life base through analysis of the questionnaires of the uterine fibroids health-related quality of life.Methods Retrospectively analyze the UFS-QOL subscales and SF-36 subscales of the 302 women with uterine fibroids who underwent ultrasound ablation in the high intensity focused ultrasound center of the First Affiliated Hospital of Chongqing Medical University in 2013.Results The Cronbach'sa of the UFS-QOL symptom severity subscale and the HRQL subscales were 0.794 and 0.953,reflecting adequate internal consistency.Except the sexual function field,the symptomatic group reported significantly greater symptom severity and worse health-related quality of life(HRQL) than the asymptomatic group(P＜ 0.05).Spearman's correlations between the symptom severity subscale and each HRQL subscales were moderately in the asymptomatic group,there was a linear positive correlation between the UFS-QOL subscales of activities,energy/mood and control(P＜ 0.05).In the symptomatic group,there was a linear negative correlation between the symptom severity subscale and the UFS-QOL subscales of concern,activities,energy/mood(P＜0.05),and there was a linear positive correlation between the UFS-QOL subscale of activities and the UFS-QOL subscales of concern,energy/mood.Conclusion Uterine fibroids related symptoms have an impact on the quality of life in women with uterine fibroids,and the assessments among the subscales of the quality of life influence each other.

Objective To explore the effect of bacillus calmette-guerin (BCG)intervention on expression of costimulatory molecules of B7-CD28/CD152 and T lymphocyte apoptosis in asthmatic mouse.Methods Twenty-seven mice were randomly divided into:asthma model group,BCG treatment group and normal control group.The mice were sensitized by ovalbumin(OVA) and 100 g/L Al(OH)3 with intraperitoneal injection,challenged with atomization inhalation,then reproduced the asthmatic models.The BCG treatment group were treated with BCG(0.025 mg,merely) subcutaneous injection 3 times before sensitization(5,3,1 day).The normal control group were treated with 9 g/L saline water taking place of OVA and Al(OH)3.CD28,CD152,CD80 and CD86 were analyzed by flow cytometry in splenic lymphocyte suspension.T lymphocyte was cultivated from peripheral blood mononuclear cell.The apoptosis ratio of T lymphocyte was counted by Comet Assay.SPSS 10.0 software was applied to analyze data.Results Contrasting asthma model group with normal control group,CD28 was up regulated(t=3.94 P0.05),CD86 was up regulated(t=2.68 P0.05).Contrasting BCG treatment group with asthma model group,T lymphocyte apoptosis rate was higher(t=3.15 P

Objective To investigate the relationship between the apoptosis,expressions of Bcl-2 and Bax protein in perihematomal brain regions of rats and neurologic dysfunctions after intracerebral hemorrhage (ICH). Methods Seventy Sprague-Dawley rats were divided randomly into two groups:an experimental group and a control group.A model of ICH was established by injection of 0.5 U bacterial collagenaseⅦinto the caudate nucleus in the rats.Neurological impairment was evaluated at 6 h,12 h,24 h,48 h,72 h,7 d and 14 d after 1CH,respectively, before the rats were sacrificed.TUNEL method was used to detect apoptosis,and SP method to detect expressions of Bcl-2 and Bax protein in the perihematomal brain tissues.Results Neurological impairment occurred in all the rats after ICH,and peaked at 48 h after ICH.The apoptosis and expressions of Bcl-2 and Bax protein were peaked at 48 h,6 h and 48 h after ICH,respectively.Conclusion The degree of the neurological impairment after ICH is parallel to that of the apoptosis.Apoptosis may play an important role in neurological impairment after ICH.

BackgroundIt has been proved that,after being forced,the biological soft tissue has stable biomechanical characteristics.However,there is rare study on corneal biomechanics.Rabbit is a main animal for experimental study in ophthalmology.But the biomechanical study of cornea in normal rabbit has not been reported.ObjectiveThis study was to investigate the biomechanical properties of normal rabbit central cornea and acquire the parameter. Methods Ten rabbits were sacrificed and the whole corneas were obtained and 20 central cornea specimens with 7 mm×5 mm of rabbit were prepared and tested on BOSE electroforce 3220-AT biomechanics machine under the room temperature and suitable humidity environment.Uniaxial tension,stress between strain,relaxation and creep were performed and the curves were drawn.The data was collected by wintest system to evaluate the biomechanical parameters of rabbit corneal tissue. ResultsThe maximum distortion intension of rabbit cornea was (7.7432±0.6099)MPa.After three cyclic loading,the stress gradually attenuated and the stress and strain flattened as the time change with the relaxation rate 30.33％.The deformation of the specimens enhanced with time decrease with the creep rate 24.33％. ConclusionsThe biomechanical characteristics of normal rabbit cornea are revealed in this study,which offer the basis for the experimental research of rabbit model aimed at corneal disease.