Objective To analyze the carbetocin combined with oxytocin for prevention of bleeding after artificial abortion .Methods 110 cases of pregnant women undergoing artificial abortion were randomly divided into observation group and control group.Observation group were treated with carbetocin combined with oxytocin treatment, the control group were treated by oxytocin treatment, the negative blood loss, menstrual recovery changes and curative effect in two groups of patients were compared.Results The total efficiency of the observation group was 96.36%, was significantly higher than the control group 78.18%, the difference was statistically significant (P<0.05), the 24h negative bleeding volume, vaginal bleeding time, menstruation time, first menstrual duration in the observation group were lower than those in control group, the difference was statistically significant (P<0.05).The vaginal bleeding time less than 7d in observation group of patients accounted for 30.91%, was significantly higher than the control group 18.18%; the time of vaginal bleeding >14d accounted for 3.64%, significantly lower than the control group 21.82%, the difference was statistically significant (P<0.05).The vaginal bloodmenstrual volume accounted for more than 9.09%, was lower than 32.73%in the control group, the difference was statistically significant (P<0.05).The sex hormone in two groups of patients before and after the treatment had no significant difference.The progesterone level in observation group was lower than the control group, estradiol level was higher than the control group, the difference was statistically significant (P<0.05), the adverse reaction rate in control group was 12.73% compared with 7.27% in the observation group, the difference was no statistically significant.Conclusion The abortion patients after treated with carbetocin combined with oxytocin treatment can reduce the amount of vaginal bleeding, shorten the bleeding time and menstrual recovery time, safe and reliable curative effect.

Aim To investigate the effect of osthole against thrombosis and platelet aggregation.Methods Rat model of artery-vein bypass thrombosis and mouse model of thrombosis by injecting collagen-adrenaline to the vein of the tail were used to measure the thrombus weight and to observe the number of dead mice in 5 min and the recovery mice from hemiplegia in 15 min. ADP,thrombin and arachidonic acid were used to induce platelet aggregation on human and the rate of platelet aggregation in 1,3 and 5 min and the maximum rate of platelet aggregation were detected.Results Osthole inhibited artery-vein bypass thrombosis and reduced thrombus weight in rats to defferent degrees,inhibited the thrombosis induced by collagen-adrenaline and reduced the death rate in 5 min and increased the recovery rate in 15 min. Osthole also inhibited human platelet aggregation induced by ADP,thrombin and arachidonic acid in vitro.The values of IC_(50 )(dose of the drug giving 50% inhibition) were 0.444 g?L~(-1) for ADP,0.186 g?L~(-1) for thrombin, 0.421 g?L~(-1) for arachidonic acid, respectively.Conclusion Osthole exert remarkable effects against thrombosis and platelet aggregation.

OBJECTIVE:To investigate the effect of rapid rehabilitation surgery assisted to fluorouracil,oxaliplatin,leucovo-rin(FOLFOX)on postoperative recovery,complications and quality of life in patients with colorectal cancer. METHODS:60 pa-tients with colorectal cancer were randomly divided into control group(30 cases)and observation group(30 cases). After resection of colorectal cancer,control group received 85 mg/m2 Oxaliplatin for injection,2 h intravenous infusion,d1+200 mg/m2 Calcium le-vofolinate for injection,intravenous infusion,once a day,d1-2+400 mg/m2 Fluorouracil injection,intravenous infusion,once a day, d1-5 then 600 mg/m2 for 22 h intravenous infusion,d1-2. Observation was additionally given rapid rehabilitation surgery. 2-week was regarded as 1 course,it lasted 12 courses. Urinary retention time,anal exhaust time,first defecation time,postoperative hospital-ization time,scores of life quality (overall health,emotional function,mental health,physiological functions,physical function,so-matic pain,vitality,social function)in 2 groups were observed,and the incidence of postoperative complications was recorded. RE-SULTS:The urinary retention time,anal exhaust time,first defecation time,postoperative hospitalization time in observation group were significantly shorter than control group,the incidence of postoperative complications in observation group was signifi-cantly lower than control group,overall health,emotional function and mental health score were significantly higher than control group,the differences were statistically significant(P<0.05),but there were no significant differences in physiological functions, physical function,somatic pain,vitality,social function score in 2 groups (P>0.05). CONCLUSIONS:Rapid rehabilitation sur-gery assisted to FOLFOX chemotherapy can reduce the incidence of postoperative complications in patients with colorectal cancer, improve quality of life,shorten hospitalization time and promote postoperative recovery.

A high performance liquid chromatographic (HPLC) method using triethylamine (TEA), copper *" acetate and L-proline as the chiral mobile phase (CMP) and the enantiomric analysis of thyroxine (T4) are described. The influence of the mobile phase parameters including the TEA concentration, manipulation pH, concentration of Cu*", concentration of L-proline, temperature of column and flow rate of eluent on the enantiomers separation of thyroxine was studied. The HPLC-CMP is a simple method for separation and determination of D，L-T4 in drug or in human plasma. It can be used to therapeutic drug or adverse drug reactions monitor.

3), the intra-day RSD was less than 10% and the inter-day RSD was less than 12%. The average recovery stood at 86.96%. CONCLUSION: The established method is simple, rapid, precise and suitable for the determination and pharmacological study of faropenem in human plasma.

Objective To investigate the association between bone mineral density (BMD) and left ventricular mass index (LVMI) in elderly men in Beijing.Methods Totally 370 elderly men with an average age of (76.6±9.3) years from the departments of gerontology were included.BMD,echocardiography measurements as well as blood chemistry were analyzed.LVMI was obtained by echocardiography.All the subjects were divided into two groups:non-LVH group (n=231) and LVH group (n =139).Differences in quantitative variables were tested by independent-sample t test.Multiple stepwise linear regression analysis were performed to identify determinants of LVMI.Results The serum creatinine concentration was significantly higher in LVH group than in non-LVH group [(97.1±43.0) μmol/L,(88.2±21.1) μmol/L (P＜0.05)].Compared with non-LVH group,LVH group showed that the lumbar spine BMD (L1-L4) were significantly lower[L1:(0.90±0.16) g/cm2 vs.(0.95±0.21) g/cm2,P=0.05; L2:(0.95±0.17) g/cm2 vs.(1.01±0.20) g/cm2,P＜0.01 ; L3:(0.99±0.19) g/cm2 vs.(1.06±0.28) g/cm2,P＜0.01] as well as the lumbar spine totalBMD [(0.97±0.18) g/cm2 vs.(1.03-1-0.26) g/cm2,P＜0.05].The femur BMD was lower in theLVH group than in non-LVH group [trochiter:(0.64±0.11) g/cm2 vs.(0.67±0.17) g/cm2,P＜0.05; inter area:(1.00±0.17) g/cm2 vs.(1.05±0.22) g/cm2,P＜0.05].Multiple stepwise linear regression analysis demonstrated that BMI (r=0.27,P＜0.01),the lumbar spine BMD (r=-0.20,P＜0.01),age (r=0.16,P＜0.05),serum creatinine (r=0.15,P＜0.05) were independently correlated with LVMI.Conclusions In elderly men in Beijing,the lumbar spine BMD is an independent correlative factor for LVMI.

Objective:To improve the TLC identification method in the quality standard for Yangyin Qingfei granules by identifying paeoniflorin and radix scrophulariae on the same condition plate. Methods:The preparation method for paeoniflorin sample solution in the original standard of TLC identification was improved, the extraction method was water-saturated n-butanol extraction, and the reference crude herb solution of radix scrophulariae was prepared. Simultaneous TLC identification of paeoniflorin and radix scrophular-iae was carried out according to the method described in the original standard. Results:The improved TLC method could be used in the simultaneous identification of paeoniflorin and radix scrophulariae, the spots were clear, and the separation and reproducibility were promising without interference. Conclusion:The improved method is more useful in the quality control of the product with simplified operation.

Objective To investigate the effect of PTEN gene in the mouse uterus during embryo implantation.Methods Real-time fluorescence quantitative PCR(FQ-PCR) was used to detect PTEN mRNA expressed in the endometria of the nonpregnant mice and the late pregnant mice(day 1,day 3,day 4,day 5 and day 7),with 20 mice sacrificed at each fixed day.Out of another 20 3-day pregnant mice,ten received PTEN antisense oligonucleotide at the horn of uterus and ten received normal saline to count the blastocysts at pregnant day 8.Results The PTEN mRNA/?-actin mRNA in pregnant mice was higher than that of nonpregnant mice,gradually hoisted as days passed by,and reached the highest at pregnant day 5.The number of blastocysts in the mice that received PTEN antisense oligonucleotide was fewer than that received normal saline.Conclusion PTEN persistently expresses in mouse endometria during the early pregnancy and maybe participate in the regulation process of mouse blastodyst implantation.

Objective - ，，，，，- - - To establish a gas chromatographic method for the determination of trans 1 1 1 4 4 4 hexafluoro 2 ［ - （）］ Methods - （） butene HFO 1366mzz E in workplace air. HFO 1366mzz E in air was directly collected with aluminum foil ， ， ， composite plastic bag separated by dimethylpolysiloxane capillary column detected by flame ionization detector and Results - （） - 3， quantified with external standard method. The linear range of HFO 1366mzz E was 6.82 68 200.00 mg/m with the 3， correlation coefficient of 0.999 9. The detection limit and the lower limit of quantitation were 0.59 and 1.98 mg/m respectively. - - - The recovery rate was within 95.45% 103.05%. The relative standard deviation of within batch precision and between batch - - ， precision were 2.26% 5.07% and 4.09% 6.82% respectively. The samples can be stored at room temperature for at least seven Conclusion ， ， days. This method is simple to use with a wide linear range low detection limit high accuracy and precision and - （） good sample stability. It can be used for the detection of HFO 1366mzz E in the air of workplace

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Carcinoma, Hepatocellular ; diagnosis ; epidemiology ; virology ; Child ; China ; epidemiology ; Female ; Genes, Viral ; Genotype ; Hepatitis B virus ; genetics ; Humans ; Liver Neoplasms ; diagnosis ; epidemiology ; virology ; Male ; Middle Aged ; Viral Load ; Young Adult