ObjectiveTo observe the clinical sense of clock-drawing test(CDT) and Mini Mental Status Examination(MMSE) in Alzheimer's disease(AD). MethodsMMSE and CDT were used to assess the intellectual ability in AD group and control group. ResultsThe orientation force, immediate memory, ability of calculation, short-term memory and speech ability in AD group were significantly decreased than that of the control group(P<0.05). The score of CDT in AD group was markedly suppression than that of control group(P<0.01). ConclusionThe CDT and MMSE are the ideal cognitive screening test to determine the degree in Alzheimer's disease.

BACKGROUND: Hematopoietic growth factors (HGFs) can be involved in different hematopoietic cells and different phase of differentiation. Erythropoietin (EPO) can promote he proliferation and differentiation of erythroid progenitor cells in vivo and in vitro, Interleukin-3 (IL-3) appears to have both the characteristics of a differentiating hormone and the ability to generate proliferation of relatively early stem cells. IL-3 acts to enhance the effect on the proliferation and differentiation of various hematopoietic cells.OBJECTIVE: To investigate the effect of HGFs and total saponins of panax ginseng (TSPG) on erythropoietic differentiation of purified CD34+ cells from human bone marrow.DESIGN: An observational comparative experiment.SETTING: Chongqing Medical University.MATERIALS: This experiment was performed in the Department of Histology and Embryology, Institute of Basic Medical Research and Key Laboratory of Biochemistry and Molecular Pharmacology in Chongqing Medical University from July 2002 to January 2004. Human bone marrow was collected from extracted ribs of patients without hematopoietic system diseases in the Department of Chest Surgery, Daping Hospital, the Third Military Medical University of Chinese PLA. Informed contents were obtained from all the patients. TSPG (purified to more than 95%) was provided by Chongqing Institute of Chinese Traditional Medicine; Fetal bovine serum (FBS), fluorescein isothiocyanate (FITC)-conjugated anti-CD34 Ab from Becton Dickinson; FITC isotype-matched mouse IgG1 and rabbit anti-CD71 Ab from Santa Cruz Biotechnology Inc; Recombinant human erythropoietin (EPO) from Amgen. rHu Flt3 Ligand (FL), rHu thrombopoietin (TPO), rHu stem cell factor (SCF) and rHu interleukin-3 (IL-3) from Santa Cruz Biotechnology Inc.METHODS: CD34+ hematopoietic stem/progenitor cells (HSC/HPC) were isolated by StemsepTM according to the strategy of negative selection on immunomagnetic separation. The sorted CD34+ cells were incubated in various combinations with IL-3+EPO, SCF+IL-3+EPO and FL+TPO+SCF+IL-3+EPO. SCF+IL-3+EPO was taken as the control group, TSPG of 10, 20, 50, 70 and 100 mg/L, then the total cell number and ratio of CD71+ cells were detected. CD34+ culture systems were added by TSPG of different dosages, those un-added by TSPG as control group. Methylcellulose culture method was used to detect the capacity of TSPG in inducing the proliferation and differentiation of CD34+ HSC/HPC [burst forming unit-erythroid (BFU-E) and colony forming unit-erythroid (CFU-E)].MAIN OUTCOME MEASURES: ① CD34+ cell proliferation in vitro in different cytokine groups; ② Effects of TSPG on inducing CD34+ cells to differentiation into CD34+ erythroid lineage hemocytes; ③ Effects of TSPG on the ability of CD34+ cells in forming erythroid lineage progenitor cells.RESULTS: ①For FL+TPO+SCF+IL-3+EPO, the mean increase in overall cell number corresponded to 546.38-fold expansion, and the ratio of CD71+ cells was increased to (61.20±5.31)%. ② TSPG 20 mg/L was identified as the most potent concentration of those tested, and the ratio of CD71+ cells was increased from (48.39±5.07)% to (56.20±1.40)%. ③ The addition of TSPG(10-50 mg/L) increased the colony production rates of BFU-E and CFU-E.CONCLUSION: A combination containing FL+TPO+SCF+IL-3+EPO may obviously induce CD34+ cells to proliferate and differentiate to erythroid lineage; TSPG may promote CD34+ cells to differentiate into erythroid lineage by cooperating with HGFs.

Congresses as Topic ; Humans ; Multiple Myeloma ; United States

Dermatitis, Occupational ; Humans ; Male

1-Alkyl-2-acetylglycerophosphocholine Esterase ; blood ; genetics ; Adolescent ; Asthma ; blood ; enzymology ; genetics ; Child ; Child, Preschool ; Female ; Gene Expression Regulation, Enzymologic ; Genotype ; Humans ; Infant ; Male ; Mutation ; Polymerase Chain Reaction

Animals ; Cell Line ; Chloride Channels ; metabolism ; Epithelial Cells ; metabolism ; Escherichia coli ; metabolism ; Kidney ; cytology ; Osmotic Pressure ; Periplasm ; metabolism ; Swine

ObjectiveTo investigate the effect of clinical pathway teaching methord in nursing practice teaching. Methods80 junior college nursing students were randomly divided into control and experimental groups. Traditional clinical teaching method was given to control group, while the clinical pathway teaching method was given to observation group. Scores of comprehensive quality after departmental rotation and satisfaction rates of nursing students to teaching method in these two groups were evaluated. ResultsThe experimental group was significantly better than the control group ( P＜0.05 ), and the difference was statistically significant. ConclusionThe clinical pathway can significantly improve the quality of nursing practice teaching.

A remarkabale inhibitory activity was exhibited by the aqueous extract from Rhizoma smilacisglabrae(RSG) against both picryl chloride(PC)- induced contact dermatitis and sheep red bloodcells (SRBC)- induced footpad reaction.The effect of RSG was displayed more distinctlywheng given after than before the 2nd antigen challenge.RSG also showed a marked anti-in-flammatory activity against xylene- induced ear and egg whiteinduced footpad edema.Addi-tionally,RSG did not show a potable influence on IgM- and IgG-PFC counts against SRBC inmice.An increase but not decrease in serum hemolysin level,however,was observed in both groupsof RSG,peralleled with the finding that hemolytic plaques in PFC test were obviously biggerthan those in control.These results suggest that RSG has a selective activity to suppress the cellularimmune response without inhibiting the humoral immune response.The suppression to cellular im-munity by RSG may be presented mainly through affecting the inflammatory process after lym-phokine release.

BACKGROUND: The source of bone marrow mesenchymal stem cells (BMSCs) is limited, and the cellular morphology,proliferation and multi-directional differentiation capacities can vary during serial passages in BMSCs in vitro.OBJECTIVE: To study the effects of fibroblast growth factor (FGF) on cellular morphology, proliferation and differentiation of serially passaged BMSCs.METHODS: (1) BMSCs were isolated from Sprague-Dawley rats and cultured. These cells were passaged six times in vitro, and the cellular morphology was observed and photographed. (2) BMSCs at passage 6 were seeded into 96-well plates and randomly divided into control group and FGF treatment group. The proliferation of cells in both groups was detected with cell counting kit-8 kit at days 1, 2, 3, 4, 5, 6, 7 after culture. (3) BMSCs at passage 6 were seeded into 6-well plates and randomly divided into control group and FGF treatment group. After 7 days treatment with growth medium or growth medium containing FGF, the cellular morphology was observed and photographed. And then the cells of both groups were treated with osteogenic induction medium, adipogenic induction medium and chondrogenic induction medium for the next 7 days. The osteogenic, adipogenic and chondrogenic related genes (RUNX2, ALP, OCN;PPARγ2, AP2, ADIPOQ; SOX9, collagen II, aggrecan) were detected with real-time PCR. The protein expressions of RUNX2, PPARγ2, SOX9 were detected with western blot assay. (4) BMSCs at passage 6 were seeded into 6-well plates and randomly divided into control group and FGF treatment group. After 7 days treatment with growth medium or growth medium containing FGF, the cells were cultured with osteogenic induction medium, adipogenic induction medium and chondrogenic induction medium for the next 14 days. Then, alizarin red S staining, oil red O staining and alcian blue staining were performed.RESULTS AND CONCLUSION: (1) After in vitro passage for six times, the cellular morphology changed obviously, and FGF treatment recovered the characteristics of primary cells. (2) Compared with the control group, the cell proliferation in the FGF treatment group was significantly increased (P < 0.05). (3) Compared with the control group, the expression of osteogenic, adipogenic and chondrogenic related genes (RUNX2, ALP, OCN; PPARγ2, AP2, ADIPOQ; SOX9, collagen II, aggrecan) was increased significantly in the FGF treatment group (P < 0.05). The protein expressions of RUNX2,PPARγ2, SOX9 were also higher in the FGF treatment group than the control group (P < 0.05). (4) Compared with the control group, the number of extracellular calcium nodules, the number of intracellular lipid droplets, and the expression of acid acidic mucopolysaccharide were significantly increased after FGF pretreatment. To conclude, FGF pretreatment can preserve the stemness of BMSCs serially passaged in vitro.

OBJECTIVE:To study the antitumor mechanism of Ruixiang Langdu(Stellera chamaejasme L ) abstracts(SCA) METHODS:SCA-contained serum was derived from mice pre-administrated with different oral dosages of SCA and at different times after administration The effects of the serum on the proliferation of K562 leukemic cells were observed with MTT assay and clone formation RESULTS:After mixing with the SCA-contained serum derived at 1,2,4,8h after giving SCA(3,6 and 12g/kg),the rates of MTT transformation and clone formation of K562 cells were decreased significantly The SCA-contained serum 12 h after giving drug was more effective than others CONCLUSION:The SCA-contained serum inhibited the proliferation of tumor cells,which may be one of its important antitumor mechanism