1.Comparison of curative effect of two treatment methods in children with bronchial asthma
Rong YU ; Cuiqin GAO ; Xinxia LI
Chinese Journal of Primary Medicine and Pharmacy 2010;17(8):1068-1070
Objective To investigate curative effect in children with bronchial asthma by two treatment methods. Methods 100 children with bronchial asthma,according to the different treatment were divided into treatment group 50 cases and control group(50 cases). The first seconds vital capacity(FEV1),peak expiratory flow (PEF) ,vital capacity 25% of the instantaneous velocity (V75), vital capacity 50% of the instantaneous velocity (V50) ,vital capacity 75% of the instantaneous velocity(V25) and their percentage the percentage of predicted value were observed. Results Total effective of 43 cases(86.0%) in treatment group after treatment 4 weeks were higher than that in control group of 28 cases (56. 5 %) (x~2 = 3. 987, P < 0.05); The percentage of FEV1 and PEF in two groups after treatment 12 weeks were significantly higher than that before treatment(x~2 = 4. 01,4. 21,4. 31,4. 08, all P < 0.05), but it between the two groups was not statistically significant (x~2 = 2. 31,2. 41, all P > 0.05); The pulmonary function of V75 、V50、V25 in two groups after treatment 12 weeks reached projected value of more than 90% ;The pulmonary function of V75 、V50、V25 after treatment compared with those before treatment were improved significantly (x~2 =4.285,4.234,4.311,4.278,all P<0.05). Conclusion The curative effect of bronchial asthma were confirmed in two methods,but treatment group were better than the control group.
2.Breeding Selenium-Enriched Yeast by Protoplast Mutagenesis.
Microbiology 1992;0(02):-
This paper studied on breeding selenium-enriched yeast by protoplast mutagenesis. A strain which the content of selenium is the highest is selected from thirteen strains yeast. The optimum conditions to form protoplast are lysed by 1 g/100 mL lywallzyme for 120 min, the formation and regeneration being 95.2% and 21.8% respectively. By mutating breed a strain of A1 which the content of selenium is 821 mg/kg and the amount of dry cell of 0.84 g/100 mL is obtained.
3.Recent advances in studies on in-situ and invasive ductal carcinoma.
Rong-ju ZHANG ; Yun NIU ; Yu-xia GAO
Chinese Journal of Pathology 2009;38(1):63-65
Aneuploidy
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Breast Neoplasms
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genetics
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metabolism
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pathology
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Carcinoma in Situ
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genetics
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metabolism
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pathology
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Carcinoma, Ductal, Breast
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genetics
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metabolism
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pathology
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Cell Movement
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DNA Methylation
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DNA, Neoplasm
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genetics
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Female
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Humans
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Membrane Proteins
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metabolism
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Neoplasm Invasiveness
4.Current research status of macular epiretinal membrane
Guo-Feng, FANG ; Rong-Yu, GAO ; Xin-Yan, XU
International Eye Science 2017;17(8):1469-1472
Epiretinal membrane (ERM) is a relatively common macular disease that forms along the surface of the internal limiting membrane (ILM) of the retina to some reason.The pathogenesis is not clear.Microincision vitrectomy surgery has been confirmed as a minimally invasive and very safe modality of treatment.Deciding when to perform a vitrectomy can be difficult.There are many factors which can affect the postoperative visual acuity,such as age,the thickness of macular,integrity of photoreceptor inner segment/outer segment (IS/OS) junction.
5.Construction of lentivirus vector containing human ?-catenin-EGFP and its expression in human hair follicle stem cells
peng-gao, YANG ; xiao-hui, HU ; feng-hou, GAO ; wei-rong, YU ; peng, XU ; yong, FANG
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(09):-
Objective To construct the lentivirus carrying human ?-catenin-EGFP(enhanced green fluorescent protein)and observe its expression in human follicle stem cells.Methods The ?-catenin gene sequence was amplified by RT-PCR from extraction of total RNA of human vascular endothelial cells.TA cloning technique was utilized to acquire gene subcloned pUCm-T-?-catenin.After transformation reaction,candidate clone was further analyzed by PCR and gene sequencing.Then the plasmid was transfected into FT293 cells.After identification by Western blotting,the plasmid was transfected into FT293 cells again for packaging.Infection titer was monitored by green EGFP expression.The expression of ?-catenin-lentivirus in human follicle stem cells were observed under inverted fluorescence microscope.Results The ?-catenin gene was cloned into the lentivirus successfully.The high expression of green fluorescence protein in FT293 cell line was found under fluorescent microscope.Viral titer checked by real-time PCR was about 2.0?108 TU/mL.When the multiplicity of infection(MOI)was 10,the infection efficiency of ?-catenin-lentivirus in human follicle stem cells was nearly 80% after infection 48 h around.After 3 weeks of continuous observation,we found the infection efficiency still keeping in the range of 80%-90%.Conclusion The lentivirus expression vector for ?-catenin was successfully constructed.It can steadily infect human follicle stem cells and the infection efficiency is considerable high.
6.Effects of trichlorfon exposure on serum paraoxonase activity of pregnant mice and development of embryos
yi-jun, ZHOU ; shu-fang, ZHOU ; rong, SHI ; yu, GAO ; yu, DING ; ying, TIAN
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(03):-
Objective To investigate the effects of maternal long-term exposure to low-dose trichlorfon on the serum paraoxonase (PON) activity of pregnant mice and development of embryos. Methods Female ICR mice (n=120) were randomly divided into control group and trichlorfon groups of different doses,and were managed by intragastric injection with trichlorfon of 0,2,10 and 50mg/kg,respectively. All the mice were managed once a day for a consecutive of 27 days,and were subjected to mating. The pregnant mice were continued to be managed with trichlorfon for 3 days,and were sacrificed on day 3 of gestation. The serum PON and acetylcholinesterase (AchE) activities were detected,and the development of embryos was evaluated. Results The serum PON activity of 2,10 and 50mg/kg trichlorfon group were (14.15?1.22),(12.78?1.80) and (10.45?1.95)IU/mL,respectively,and that of 50mg/kg trichlorfon group was significantly lower than that of control group [(13.37?2.31)IU/mL] (P0.05),while the the percentage of abnormal embryos of 50mg/kg trichlorfon group had an increased tendency. Conclusion Long-term exposure to low-dose trichlorfon can inhibit serum PON and AchE activity in pregnant mice without obvious effect on the development of embryos.
7.Virtual screening and activity study of antiviral compounds targeting inosine 5′-monophosphate dehydrogenase
Shi-bo KOU ; Rong-mei GAO ; Hong YI ; Lian-qi SUN ; Yu-huan LI ; Zhuo-rong LI
Acta Pharmaceutica Sinica 2022;57(10):3011-3018
Inosine 5′-monophosphate dehydrogenase (IMPDH) is a key enzyme catalyzing the rate-limiting step of
8.Influence of Different Fixing Methods on Cardiac Index in C57 Mice of Wild Type andβ-adrenoceptor Knockout Mice
Xia LI ; Junhong GAO ; Jingjun ZHAO ; Hui BEN ; Xiaochun YU ; Bing ZHU ; Peijing RONG
World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(2):249-253
This study was aimed to choose the best fixing method of electrocardiogram (ECG) measurement in mice by comparison of ECG index in three different fixing ways. Changes of ST amplitude and heart rate in ECG were measured in the self-made mouse sets, tape bundling or breathing anesthesia state. The amplitudes of the ST seg-ment in C57BL6 mice and β1/β2-AR double knockout mice were elevated in tape bundling or breathing anesthesia state in comparison with that in the self-made mouse sets (all P < 0.01). The heart rate under the tape bundling state in C57BL6 mice was significantly faster than that in the self-made mouse sets (P< 0.05 or P< 0.01). And the heart rate under anesthesia in β1/β2-AR double knockout mice was significantly slower than that in tape bundling state or the self-made mouse sets (both P < 0.01). It was concluded that relative to the binding and anesthesia method, fixing mouse in the self-made mouse sets, which can more truly reflect the physiological or pathological states, is a relatively good fixing method of ECG measurement in experimental study.
9.Impact of three to four cycles of neoadjuvant chemotherapy on survival of patients with N2-N3 nasopharyngeal carcinoma
Jiawang WEI ; Rong HUANG ; Xin YU ; Qiaoxuan WANG ; Weiwei XIAO ; Lixia LU ; Yuanhong GAO ; Hui CHANG
Chinese Journal of Radiation Oncology 2017;26(4):380-383
Objective To evaluate the impact of three to four cycles of neoadjuvant chemotherapy (NACT) on the survival of patients with N2-N3 nasopharyngeal carcinoma (NPC).Methods The clinical data of 915 patients with T1-4N2-3M0 NPC from 2007 to 2010 were retrospectively analyzed.A total of 179 patients treated with 3-4 cycles of NACT (NACT≥3 group) were matched with 358 patients treated with 2 cycles of NACT (NACT=2 group) and 179 patients treated without NACT (NACT =0 group,concurrent chemoradiotherapy group) for age,N stage,pathological subtype,and NACT regimen.The Kaplan-Meier method was used to calculate overall survival (OS),disease-free survival (DFS),recurrence-free survival (RFS),and distant metastasis-free survival (DMFS) rates,the log-rank test was used for survival difference analysis and univariate prognostic analysis,and the Cox proportional hazards model was used for multivariate prognostic analysis.Results For the NACT≥ 3,NACT =2,and NACT =0 groups,the 5-year OS rates were 89.4%,81.6%,and 73.7%,respectively (P=O.000),the 5-year DFS rates were 83.2%,69.8%,and 64.2%,respectively (P=O.000),the 5-year RFS rates were 86.0%,76.0%,and 69.3%,respectively (P=0.001),and the 5-year DMFS rates were 86.6%,76.0%,and 68.3%,respectively (P=0.000).Three to four cycles of NACT was an independent protective factor for OS,DFS,RFS,and DMFS in patients with N2-N3 NPC.Conclusion Three to four cycles of NACT can significantly improve the survival of patients with N2-N3 NPC.
10.The Value of MCA and UtA Doppler Parameters for the Predication of Fetus Hypoxia
Xinru GAO ; Peili AN ; Yingjin WANG ; Yu ZHANG ; Rong ZHANG ; Shigang MU ; Hong AI
Chinese Journal of Primary Medicine and Pharmacy 2010;17(23):3169-3171,后插1
Objective To evaluate the dinical value of MCA and UtA Doppler parameters for the predication of fetus hypoxia. Methods407 singleton pregnancies at the third trimester were underwent Color Doppler,of which 67 cases were in high risk group and 340 cases were in control group.UtA-PI、UtA-RI、UtA-S/D、MCA-PI、MCA-RI and MCA-S/D were all measured.The ratio of UtA to MCA was calculated.All parameters were analyzed. ResultsIn high risk group,UtA-PI,UmA-RI and UtA-S/D were all higher than that in control group(all P<0.01),MCA-PI is lower than that in control group(P<0.05),and the ratio of UtA to MCA was higher than that in control group(all P<0.01).By the standards of(UtA/MCA)-PI ≥ 0.72,(UtA/MCA)-RI ≥ 0.81,and(UtA/MCA)-S/D ≥ 0.74,the predication sensitivity of fetus hypoxia was 63.3%,and the specificity was 90.2%. ConclusionThe ratio of UtA to MCA could predict fetus hypoxia.