Adenocarcinoma, Sebaceous ; pathology ; Humans ; Male ; Middle Aged ; Parotid Neoplasms ; pathology

OBJECTIVE: The latency-intensity function (LIF) of wave V from click ABR of some deaf children showed great variation. We attempted to find out the intrinsic reasons. METHOD: The children recieved tone-burst ABR test. RESULT: Frequencies from 0.5-4.0 kHz have been tested and the thresholds of tone-burst ABR were recorded. The average thresholds of steeply LIF children at 0.5-4.0 kHz were (93.13 ± 7.04), (79.37 ± 7.72), (69.38 ± 8.54) (66.25 ± 8.06) dB respectively, while the average thresholds of shallower LIF children at these 4 frequencies were (65.00 ± 7.32) (68.13 ± 6.55) (70.63 ± 6.80) (78.12 ± 8.34) dB respectively. CONCLUSION The results imply that the child with steeply LIF may have more hearing loss at frequencies 0.5 and 1 kHz than those with shallower LIF. LIF may predict the audiogram configuration.
Audiometry, Pure-Tone ; Auditory Threshold ; Child ; Evoked Potentials, Auditory, Brain Stem ; Hearing Loss ; diagnosis ; Humans

Objective To investigate the effect of enamel matrix proteins (EMPs) on gene expression in human bone marrow stromal cells (hBMSCs) by cDNA microarray. Methods hBMSCs were cultured in vitro by whole bone marrow technique. Those stimulated with EMPs at a concentration of 200 ?g/mL in vitro were classified as test group, and those normally cultured were served as control. Afer culturing for 5 days, a cDNA microarray containing 4 096 genes was used to detect and analyze the gene expression. Four differentially-expressed genes in cDNA microarray were determined by real-time PCR for validation of the microarray data. Results Twenty-seven genes of hBMSCs were differentially expressed in the presence of EMPs, among which 18 were up-regulated and 9 down-regulated. The result of real-time PCR was in accordance with that of the cDNA microarray. Conclusion cDNA microarray technique can screen the differentially-expressed genes in hBMSCs treated with EMPs, which lays a foundation for the research of molecular mechanism of EMPs in inducing the regeneration of periodontal tissue.

Drugs, Chinese Herbal ; pharmacology ; Embryo Implantation ; drug effects ; Endometrium ; drug effects ; physiology ; Female ; Humans

Objective To explore the relationship of the microvessel density(MVD) and the expression of vascular endothelial cell growth factor(VEGF) with the pathological types and clinical stages of gastric cancers.Methods The MVD and expression of VEGF in 45 patients with gastric cancer were assayed by using SP immunohistochemical method.The relationship of the MVD and VEGF with the pathological TNM types and clinical stages of gastric cancer was analyzed.Results In the marginal area where the gastric cancer grew actively,the microvessel density(MVD) was the highest;the expression of vascular endothelial cell growth factor(VEGF) in the cancerous cell plasm was significantly higher than that in the neighboring noncancer tissues(P

Objective To investigate the effect of H.pylori infection on cyclooxygenase-2(COX-2)expression in gastric antral mucosa. Methods The expression of COX-2 in 20 patients with H.py lori -infected chronic gastritis and in 14 with uninfected chronic gastritis w ere investigated by immunohistochemical method. The expressions of COX-2 in the antral mucosa were compared before and after H.pylori eradication. Results The mean percentage of the cells stained with COX-2 was s ignificantly higher in H.pylori -infected mucosa than that in uninfected mucosa ( P

Objective:To establish a method for the simultaneous determination of fluoxertine and olanzapine in capsules by re-versed-phase high performance liquid chromatography ( RP-HPLC) . Methods:The successful separation of fluoxertine and olanzapine was achieved on a Phenomenex C18 column (250 mm × 4. 6 mm, 5 μm) with 10 mmol·L-1 potassium dihydrogen phosphate buffer (pH 4. 0)-acetonitrile-methanol (55 ∶40 ∶5, v/v/v) as the mobile phase. The flow rate was 1. 0 ml·min-1, the detection wave-length was 227 nm, the column temperature was 30℃, and the injection volume was 20 μl. Results:Fluoxertine could be well separa-ted from olanzapine under the chromatographic conditions. The linearity between the peak areas and the concentrations was observed within the range of 5. 0-80. 0 mg·L-1(r=0. 999 9) for fluoxertine and 1. 2-19. 2 mg·L-1(r=0. 999 9) for olanzapine. The mean recovery of fluoxertine and olanzapine was 99. 9%(RSD=0. 94%, n=9) and 100. 2%(RSD=2. 08%, n=9), respectively. Con-clusion:The method is simple, sensitive and accurate, and it can be applied in the content determination of fluoxertine and olanzapine in capsules.

Objective:To compare the similarity of dissolution curves of domestic ibuprofen suspension and the imported prepara-tion to provide the basis for the comprehensive quality evaluation of ibuprofen suspension. Methods: The in vitro dissolution of the products from four different manufacturers was investigated in pH 7. 2 phosphate buffer solution. The similarity of dissolution behavior was compared with that of the imported preparation in pH 1. 2 hydrochloric acid solution, pH 4. 5 acetate buffer solution, pH 6. 8 phos-phate buffer solution, pH 7. 2 phosphate buffer solution and water, respectively. Results:The dissolution rate of ibuprofen suspension from the four different manufacturers reached above 80% in 60 min. The dissolution profiles of ibuprofen suspension from two different manufacturers were similar with that of the imported preparation. Conclusion: The dissolution behavior of ibuprofen suspension from different manufacturers is significantly different.

Aim To understand the changing aspects of CAP-administration on expression of cytochrome C oxidase(COX) subunits I and IV and their mechanisms regulated by gene expression encoded by mtDNA and nDNA. Methods Wistar rats were randomly divided into control and CAP groups, Rats were administrated CAP(100 mg?kg -1, intraperitoneal injection) every 12 hours for 7 days before sacrificed by decapitation. Rat brain was removed and the cerebral cortex mitochondria was isolated by centrifugation programme. The protein content of COX subunitⅠand Ⅳ in mitochondria and NRF-1 in cerebral tissues was detected by Western blot analysis. And mRNA state levels of COXⅠ, COXⅣ, mtTFA and NRF-1 in tissues were determined by RT-PCR.Results Compared with C group, a decreased protein content of COX subunitⅠand an elevated ratio of subunit Ⅳ/Ⅰwas observed in CAP group, The protein content of COX subunit Ⅳ and NRF-1 as well as COXⅠ,Ⅳ,NRF-1 and mtTFA mRNA state level was not unchanged between the two groups. Conclusion The change of content of COX subunitⅠprotein in mitochondria from cerebral cortex showed there is no regulation of feedback to mitochondrial and nuclear transcription. The nuclear genomes expression does not correspond to mitochondrial expression in CAP-administrated rats.

Objective To understand the changing aspects of CAP-administration on mitochondrial oxidative phosphorylation function and cytochrome c oxidase (COX) activity during hypoxia exposure and their mechanisms. Methods Except the control group (C group), adult male Wistar rats were exposed to a hypobaric chamber simulated 5 000-meter high altitude for 23 h every day for 0, 1, 5, 15, 30 d (H_ 0, 1, 5, 15, 30 ) respectively and administrated CAP (100 mg/kg, intraperitoneal injection) every 12 h for 7 d before sacrificed by decapitation. Mitochondrial respiratory function and COX activity were measured by Clark oxygen electrode and polarography, respectively. Results As compared with C group, mitochondrial state 3 respiration (ST_3) and respiratory control rate (RCR) and oxidative phosphorylation rate (ORP) and COX activity in H_0+CAP group all decreased significantly, but by prolonging hypoxia exposure increased and restored to the control level. Conclusion Mitochondrial respiratory function, oxidative phosphorylation efficiency and COX activity in rat brain could improve by administrating CAP during hypoxia exposure and almost reach to the control level by prolonging hypoxia exposure.