The fibrovascular membrane of proliferative diabetic retinopathy(PDR)cause severe damage to the structure and function of eye.The pathogenesis and development of fibrovascular membrane are associated with muhipie cellular factors,such as transforming growth factor(TGF),connective tissue growth factor(CTGF),basic fibroblast growth factor(bFGF),vascular endothelial growth factor(VEGF),platelet-derived growth factor(PDGF),tumor necrosis factor(TNF),extracellular matrix(ECM),matrix metalloproteinases(MMPs),endothelin and chemotactic factor,etc..So,the molecular mechanism of fibrovascular membrane of PDR is extremely complex.The relationship of pathogenesis on the fibrosis of PDR to cytokines,extracellular matrix,MMPs and other factors were summarized.

DNA, Neoplasm ; analysis ; Female ; Flow Cytometry ; Humans ; Hydatidiform Mole ; diagnosis ; genetics ; In Situ Hybridization, Fluorescence ; Microsatellite Repeats ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Pregnancy ; Triploidy ; Uterine Neoplasms ; diagnosis ; genetics

Choroidal neovascularization (CNV) ithe leading cause of vision disordecaused by variouretinal diseases.Apresent,many therapeutimethodare employed clinically,such aphotodynamitherapy (PDT),anti-vasculaendothelial growth facto(anti-VEGF) and transpupillary thermotherapy (TTT).However,none of them can cure CNV thoroughly and repeated treatmenirequired usually.The reason forecurrenCNV istill unclear.Choroidal hypoperfusion associated with Pdmay be one of the reasons.The purpose of thireview ito discusthe problem of choroidal hypoperfusion associated with PDfoCNV awell aitimpacon the eye and possible solutions.Thipapepresentevidenceof choroidal hypoperfusion aftePDand itrelationship with clinical outcomes.Meanwhile,the effecof combination therapy iassessed.Finally,low-fluence Pdirecommended apotential method to reduce choroidal hypoperfusion.

Aim To examine the protective effects of allopregnanolone against seizure on different animal models.Methods The protective effects of allopregnanolone against maximal electrical seizure (MES) and picrotoxin-induced seizure were studied in C57 mice 15 minutes after vehicle or drug intraperitoneal administration.Results In the MES test, we found that pretreatment with the phenobarbital or allopregnanolone produced a dose-dependent protective effect against seizure. The potency (ED50 value) of phenobarbital in the MES test was 2.40 mg?kg-1, with 95% confidence interval range from 1.22 to 4.72 mg?kg-1. The potency (ED50 value) of allopregnanolone in the MES test was 0.086 mg?kg-1, with 95% confidence interval range from 0.037 to 0.201 mg?kg-1, which was significantly higher than that of phenobarbital (P

Biomarkers, Tumor ; blood ; DNA, Neoplasm ; blood ; Humans ; MicroRNAs ; blood ; Molecular Diagnostic Techniques ; Neoplasms ; blood ; diagnosis ; Nucleic Acids ; blood

Bone marrow mesenchymal stem cells (MSC) are the non-hematopoietic cellular component in the bone marrow that have multiple differentiation potency. MSC play a key role in the regulation of bone marrow hematopoietic niche and modulation of immune function through various mechanisms. They are currently recognized as a promising cell source in tissue engineering, a vehicle in gene therapy and a powerful tool in the management of graft-versus-host disease after allogeneic stem cell transplantation. In this review some momentous aspects regarding the current status and potential clinical applications of MSC in hematopoietic stem cell transplantation, aplastic anemia and multiple myeloma were summarized.

Objective:To clone interleukin 4 cDNA of Tibet pig.Methods:To amplify cDNA,using RT PCR from the total RNA from the lymphocyte stimulated by 10 ?g/ml Con A in vitro,to subclone into pMD T vector,and then to determine it by digesting and sequencing.Results:The cloned Tibet pig interleukin 4 was 98% homologus to the necleotide acids of Chenghua pig a special specie of pig in Sichuan province and 99% homologus to that of hybrid of Landrance with Chanbai pig.Conclusion:IL 4 of Tibet pig was successfully cloned from the lymphocyte after stimulating 70 h with ConA.

OBJECTIVE: To provide references for the improvement of categorized management of drugs in China. METHODS: The problems in China's categorized management of drugs were analyzed by drawing a comparison between China and USA in the categorized management of drugs. RESULT & CONCLUSION: China should draw on useful experiences from the developed countries to improve the categorized management of drugs from multi-aspects.

Bronchopulmonary dysplasia( BPD) is a chronic lung disease that commonly occurs in pre-term. Since the 1980s,with the advances in perinatal care,survival rate of preterm has been raised,while BPD continues to be a significant cause of morbidity and mortality for premature infants. However,the treatment effect of BPD is unsatisfactory. It is urgent to search for innovative ways to reduce the respiratory injury that caused by BPD as well as to improve the quality of life. In recent years,mesenchymal stem cells′( MSC) research has pro-vided a new way for the treatment of BPD. This review primarily summarizes the current status of the MSC ther-apies and its possible mechanism on BPD.