Objective:To establish a method for determining the concent of ferulic acid in compound Yangjiao soft capsules with HPLC.Method:To determine with HPLC using C18 column(250mm?4.6mm,5?m),methanol-1% acetic acid solution(37:63) as mobile phase.The flow rate was 1.0 ml/min and the detecting wavelength was 320nm.Results: Ferulic acid had good linear relation within the range from 6 to 60?g/min,r =0.9995.The average recovery rate was 100.3%,RSD=1.68%(n=9).Conclusion:The method is with good resolution,accurate and reproducible,which can be used for determining the content of ferulic acid in compound Yangjiao soft capsules.

AIM: To develop a method for determining contents of ginsenoside Rb_1,ginsenoside Rg_1 and notoginsenoside R_1 in Sanxue Mingmu Tablet(Radix et Rhizoma Notoginseng,Pheretima,Herba Leonuri,Rhizoma Imperatae,etc.). METHODS: The contents of ginsenoside Rb_1,ginsenoside Rg_1 and notoginsenoside R_1 were separated by gradient elution on Hypersil ODS C_(18) column(5 ?m ? 4.6 mm?100 mm) as stationary phase;acetonitrile and water as gradient mixed mobile phase;detected at 203 nm wavelength;(25 ?C) of column temperature and 1.0 mL/min of mobile rate. RESULTS: Ginsenoside Rb_1,ginsenoside Rg_1 and notoginsenoside R_1 had good linearities(r=0.999 9;n=6) at the ranges of 1.480 0-14.800 ng;0.372 0-3.720 ng and 1.072 0-10.720 ng.The average recoveries were 98.44%(RSD=0.96%);99.02%(RSD=0.94%);97.95%(RSD=(1.02%)),respectively. CONCLUSION: This method is high in column efficiency and satisfactory for isolation and repeatability,the result is accurate.It can be adopted for quality control of Sanxue Mingmu Tablet.

Objective To investigate the effects of Ziyin Huoxue Jiedu Chinese herbs(ZYHXJD) on vascular endothelial cells injured by glucose,insulin and low-density lipoprotein.Methods Human umbilical vein endothelial cell line ECV-304 was exposed to different concentration of glucose,insulin and oxidized low-density lipoprotein(Ox-LDL),and the effects of ZYHXJD on the injured vascular endothelial cells were investigated.The cells in each group were cultured for additional 48 h.And then,cell viability was examined,and the supernatants were used to determine the contents of tissue plaminogen activator(tPA),plasminogen activator inhibitor(PAI),and intercellular adhesion molecule-1(ICAM-1) respectively.Results The viability of the cells in the model group decreased markedly(P

Objective To discuss the necessity of antibiotics usage in septoplasty , and to provide evidences for the standardization of the therapy and rational use of antibiotics in septoplasty. Methods Eighty-seven patients who had undergone septoplasty were randomly divided into three groups: Group A: without antibiotics; Group B: antibiotics only preoperative prophylaxis; Group C: antibiotics both preoperative and postoperative for five days. The clinical effect and complication rate were compared among three groups. Results There were no statistical significance among three groups for clinical effect and complication rate (P > 0.05). Conclusions The infection rate in septoplasty is very low , and the use of prophylactic antibiotics in elective septoplasty can neither improve efficacy nor reduce postoperative complications , so it is not essential.

Objective: To investigate the effect of fusion protein SD (SH2-DED) on the K562 leukemia subcutaneous xenografts in nude mice. Methods: The models of K562 leukemia subcutaneous xenografts in nude mice based on pretreatment and treatment with recombinant adenovirus were established. In the pretreatment model, the K562 cells pretreated with recombinant adenoviruse Ad5F35-SD or its mutant Ad5F35-SmD were subcutaneously injected into the nude mice; in the treatment model, the K562 cells were firstly subcutaneously injected into the nude mice to induce the subcutaneous xenografts, and then the recombinant adenoviruse Ad5F35-SD or its mutant Ad5F35-SmD was intratumorally injected into the xenografts. The growth of the subcutaneous xenografts and the morphological changes of the tumor cells were observed. The apoptosis of the tumor cells in subcutaneous xenografts was detected by TUNEL method and observed under a transmission electron microscope. The expression levels of caspase-3 and caspase-8 proteins in the xenografts were examined by immunohistochemistry. Results: In the treatment model, the volume of subcutaneous xenografts was significantly inhibited by Ad5F35-SD treatment, and the pathological results showed nuclear condensation and deep staining of cytoplasm. The apoptosis of tumor cells was confirmed by TUNEL method and transmission electron microscopy. The expressions of apoptosis-associated proteins caspase-3 and caspase-8 were increased. In the pretreatment model, the growth of xenografts was also inhibited by pretreatment with Ad5F35-SD. Conclusion: Recombinant adenovirus Ad5F35-SD can inhibit the tumorigenicity of K562 cells and the growth of tumor xenografts in nude mice, and promote the apoptosis of tumor cells. © 2012 by Tumor.

OBJECTIVE:To prepare Idebenone solid dispersion,and to investigate its dissolution rate in vitro. METHODS:Us-ing Poloxamer 407(P407)as carrier,the influence of preparation methods(solvent method,melting method)and the ratio of the drug to P407(1∶1,1∶3,1∶8)on the dissolution of drug were investigated by single factor design. The state of idebenone in ma-trix of solid dispersion was further determined by using differential scanning calorimetry (DSC) and X-ray powder diffraction (XRD). RESULTS:Idebenone solid dispersion prepared by solvent method(the ratio of the drug to poloxamer was 1∶3)showed dissolution rate of 80%. The majority of idebenone existed in the solid dispersion at amorphous forms or molecular state. CONCLU-SIONS:Idebenone solid dispersions with high dissolution rate in vitro is prepared successfully.

BACKGROUND: Siberian solomonseal rhizome is a sort of Chinese traditional medicine for anti-senilism. The effective component, poly gonati polysaccharide, has the effects of reducing blood glucose and glycosylhemoglobin.OBJECTIVE: To assay regulative effect of polygonati polysaccharide on expression of the key substance of non-enzymic glycosylation of proteinsglycosylated end-product receptor mRNA by reverse transcriptase polymerase chain reaction, so as to develop effective inhibitor for non-enzymic glycosylation of proteins and provide experimental evidences for preventing diabetes and its complications.DESIGN: Randomized control animal trial SETTING: Department of Geriatrics, the Second Xiangya Hospital, Central South University; Department of Cardiology, Haikou Hospital Affiliated to Xiangya Medical College, Central South University.MATERIALS: The experiment was completed in Animal Room of Second Xiangya Hospital of Central South University form March to June 2004. A total of 30 BALB/C mice of clean grade were selected and randomly divided into normal control group, model control group and polygonati polysaccharide group, with 10 in each group.METHODS: Diabetic models were established by intraperitoneal injection with streptozotocin to mice in model control group and polygonati polysaccharide group. Model establishment would be regard as successful if blood glucose of mouse was 8.0 mmoL/L or above. Mice in polygonati polysaccharide group were treated with polygonati polysaccharide (2 mL/kg per day), while mice in normal control group and model control group were treated with injection of 0.5 mL water once a day for 12 consecutive weeks.After medicine had been given to the mice, they were put to death by decapitation. Reverse transcriptase polymerase chain reaction was used to assay expression of glycosylated end-product receptor mRNA in cardiac and renal tissues of experimental animals.MAIN OUTCOME MEASURES: ① Observation of general situation of mice in each group 12 weeks later after model establishment. ② Change of blood glucose of mice in each group before and after model establishment.③ Gel electrophoretic maps of glycosylated end-product receptor mRNA in cardiac and renal tissue of mice in each group. ④ Semi-quantitative assay of glycosylated end-product receptor in cardiac and renal tissue of mice in each groupRESULTS: All the 30 mice entered the results analysis. ① Observation of general situation of mice in each group 12 weeks later after model establishment: mice in normal control group gained weight and moved freely; mice in model control group manifested the symptoms of losing weight, polyuria, listlessness, lags in response etc.; mice in polygonati polysaccharide group manifested milder symptom of polyuria and more sensitive in responses as compared with model control group.② Changes of blood glucose of mice in each group before and after model establishment: blood glucose levels were similar between normal control group and model control group before and after model establishment (P ＞ 0.05), while blood glucose in polygonati polysaccharide group significantly decreased 12 weeks later after model establishment [(10.05±1.16), (7.18±0.84) mmoL/L, P ＜ 0.05]. ③ Gel electrophoretic maps of glycosylated end-product receptor mRNA in cardiac and renal tissue of mice in each group: Compared with normal control group, the expression of glycosylated end-product receptor mRNA increased in cardiac and renal tissue of mice in model control group, while the expression in polygonati polysaccharide group significantly decreased as compared with model control group. ④ Semi-quantitative assay of glycosylated end-product receptor in cardiac and renal tissue of mice in each group: the relative value of glycosylated end-product receptor to β-actin in cardiac and renal tissue of mice in model control group was significantly higher than normal control group (P ＜ 0.01); however, the relative value of polygunati polysaccharide group significantly decreased as compared with model control group (0.760±0.121,0.998±0.202;0.609±0.146;0.765±0.113; P ＜ 0.05).CONCLUSION: Besides reducing blood glucose, polygonati polysaccharide can significantly down regulate high expression of glycosylated endproduct receptor mRNA in cardiac and renal tissue of mice with diabetes, so as to inhibit the combining sites for glycosylated end-products and a series of cytobiological reactions after combined with their receptors, and protect the target organs and tissues from injuring by hyperglycemia.

Objective To evaluate the value of two-dimensional strain imaging in assessing right ventricular function of recipient fetus in TTTS pregnancies.Methods Sixteen TTTS pregnancies and 19 normal monochorionic diamniotic pregnancies(controls) were included.Doppler studies of the umbilical artery,umbilical vein,ductus venosus,middle cerebral artery,atrioventricular valve and semilunar value were recorded in both fetus,and myocardial performance index of both ventricles was calculated.Longitudinal peak systolic strain of right ventricular were calculated and compared between recipient fetus and other fetus.Results Cardiothoracic ratio and myocardial performance index of right ventricular showed significant differences between recipient fetus and controls.Right ventricular strain was decreased in recipient fetus compared with controls.Conclusions Two-dimensional strain imaging can be used to evaluate right ventricular myocardial function in the recipient fetus of TTTS.

Butyric acid can be used to produce cellulose butyrate fiber and ester derivatives,and to be applied in foodstuffs and perfume industries.Recent researches have found that butyric acid is a preferred carbon source for colonic epithelial cells,and can inhibit histone deacetylase,showing great anticancer potentials.With more and more functions of butyric acid being found and applied in bio-related fields,and with consumer's growing preference to bioproducts,biotechnological production of butyric acid will receive more competence than petroleum-based chemical synthesis.Low product concentration and poor selectivity are presently the main restricting factors.Workers have made considerable progress on more cheep carbon sources,optimization of fermentation process,simplifying downstream processing,and genetic engineering of producing strains.Any achievement on these aspects in the future can contribute to put fermentation of butyric acid into industry.

Objective To demonstrate the superiority and feasibility of using bioluminescent image to monitor the islet graft after islet transplantation.Methods Diabetic models were established by intraperitoneal injection of streptomycin into mature male C57BL/6 mice.Islets were harvested from the pancreas of C57BL/6 and Bclb/c mice by digestion and purification,and transfected with Lueiferase gene.The mouse diabetic models were divided into iso-transplantaion group (n=20) and allo-transplantation group (n=7).The islets of C57BL/6 were transplanted into iso-transplantaion mice with different doses (10,50,110 and 200,n=5 in every dose),and Bclb/c mouse islets were transplanted into allo-transplantation group.The islets were transplanted into the subcutaneous fat tis- sue near left scapula.The receptor mice were scanned with CCD camera to get bioluminescent images at different scheduled time points,and the changes in random blood glucose of allo-transplantation group were observed.Results On day 6 after transplantation,the scanning image showed that the pi- xel intensity from the region of interest (ROI) was increased with the increased number of islet grafts and they had a positive correlation.The random blood glucose was reduced to the normal level in the first 2 days,and then increased again to the diabetic level on 11 days averagely,while pixel intensity from the ROI reached the peak on day 6-7,and then reduced rapidly after islet transplantation in allo- transplantation group.The beginning of pixel intensity reduction occurred on day (6.14?0.90), while that of the random blood glucose raise occurred on day (10.00?0.82) after transplantation,and the former alteration occurred obviously earlier than the latter (P