Objective To explore the feasibility of two-day laparoscopic cholecysteetomy(LC).Methods The clinical data of 50 patients were analyzed retrospectively.Results All the operations were successful.Condu-sions Two-day laparoscopic cholecystectomy is deserved to spresd which is also safe,available and higher bed rota-tion as well as great patient satisfaction.

Objective:To clone interleukin 4 cDNA of Tibet pig.Methods:To amplify cDNA,using RT PCR from the total RNA from the lymphocyte stimulated by 10 ?g/ml Con A in vitro,to subclone into pMD T vector,and then to determine it by digesting and sequencing.Results:The cloned Tibet pig interleukin 4 was 98% homologus to the necleotide acids of Chenghua pig a special specie of pig in Sichuan province and 99% homologus to that of hybrid of Landrance with Chanbai pig.Conclusion:IL 4 of Tibet pig was successfully cloned from the lymphocyte after stimulating 70 h with ConA.

Objective To explore the value on the treatment of severe acute pancreatitis (SAP) by hemofiltration combined with Dan Sen and catheterizing drainage through laparoscope. Methods Thirty patients of SAP were divided into two groups. On the basis of routine treatment(supportive treatment,suppressing excrine of pancreas stilamin,trypsin inhibitor and antibiotics),hemofiltration,catheterizing drainage,stilamin and Dan Sen were added in group A,while only routine treatment were administered in group B. TNF,IL-6,IL-8,score of Balthazar CT and score of APACHEⅡ were studied in both groups. Results The value of TNF,IL-6,IL-8,score of Balthazar CT and score of APACHEⅡ in group A were significantly lower than those in group B(P

Objective To investigate curative effect in children with bronchial asthma by two treatment methods. Methods 100 children with bronchial asthma,according to the different treatment were divided into treatment group 50 cases and control group(50 cases). The first seconds vital capacity(FEV1),peak expiratory flow (PEF) ,vital capacity 25% of the instantaneous velocity (V75), vital capacity 50% of the instantaneous velocity (V50) ,vital capacity 75% of the instantaneous velocity(V25) and their percentage the percentage of predicted value were observed. Results Total effective of 43 cases(86.0%) in treatment group after treatment 4 weeks were higher than that in control group of 28 cases (56. 5 %) (x~2 = 3. 987, P ＜ 0.05); The percentage of FEV1 and PEF in two groups after treatment 12 weeks were significantly higher than that before treatment(x~2 = 4. 01,4. 21,4. 31,4. 08, all P ＜ 0.05), but it between the two groups was not statistically significant (x~2 = 2. 31,2. 41, all P ＞ 0.05); The pulmonary function of V75 、V50、V25 in two groups after treatment 12 weeks reached projected value of more than 90% ;The pulmonary function of V75 、V50、V25 after treatment compared with those before treatment were improved significantly (x~2 =4.285,4.234,4.311,4.278,all P＜0.05). Conclusion The curative effect of bronchial asthma were confirmed in two methods,but treatment group were better than the control group.

This paper studied on breeding selenium-enriched yeast by protoplast mutagenesis. A strain which the content of selenium is the highest is selected from thirteen strains yeast. The optimum conditions to form protoplast are lysed by 1 g/100 mL lywallzyme for 120 min, the formation and regeneration being 95.2% and 21.8% respectively. By mutating breed a strain of A1 which the content of selenium is 821 mg/kg and the amount of dry cell of 0.84 g/100 mL is obtained.

Objective To evaluate the treatment of postoperative bile-duct residual stones with rigid cholangioscopy. Methods Three hundred and twenty-seven cases of bile duct residual stones were treated with rigid cholangioscopy. As a route for the rigid cholangioscopy, the T-tube tract (315 cases), the U-tube tract (5 cases) and the jejunostomy tube tract of efferent limb (7 cases) were used. Results Four hundred and eighty-six rigid cholangioscopic sessions were carried out, 1.5 sessions on the average for each patient. Treatment was successful in all but one patient in whom rigid cholangioscopic access to bile duct was difficult since the T-tube tract was too long. The rate of complete removal of the stones was 95.4%. There were no serious complications. Conclusions It′s possible to pass the instrument into the common bile duct and the majority of the intrahepatic bile duct. Many of the problems associated with residual stones can be overcome by this method and good results achieved. This technique seems to be a useful new alternative in patients with difficult retained bile duct stones.

Aged ; Esophagus ; Foreign Bodies ; complications ; diagnosis ; Hoarseness ; diagnosis ; etiology ; Humans ; Male

Objective To observe the effect of gheocorticoid by oral medication or intrathoracic iniection on the expression of IL-6,IL-8 and sIL-2R in gerum and pleural fluid in patients of tuberculous pleurisy.Methods Twenty tuberculous pleurisy patients were treated with ghcocortieoid by oral medication (oral group)and 20 cases by intrathoracic injection(injection group).ELISA wag employed to detect the levels of IL-6,IL-8 and sIL-2R in serum and pleural fluid before treatment and 3,6 and 9 days after treatment.Results The levels of serum IL-6 and IL-8 decreased and sIL-2R increased obviously in injection group at 3,6 and 9 days after treatment(P＜0.01).Meanwhile the levels of IL-6 and IL-8 in pleural fluid decreased and sIL-2R increased obviously(P＜0.01).The levels of IL-6 and IL-8 were significantly lower and the levels of aIL-2R were significantly higher in injection group than those in oral group(P＜0.01).One month after treatment,the rate of complete absorpion of pleural fluid was higher in injection group(80%)than thatin oral group(45%),the rate of pachynsis pleurae was lower in injection group(10%)than that in oral group(40%),P＜0.05.Conclusions Intrathoracic injection of ghcocorticoid shows stronger suppression of cellular immune function in cavum pleurae and weaker suppression of the general immune sysmm than oral medication.Intrathoracic injection of glucocorticoid can increase the therapeutic effect and decrease side effect in tuberculous pleurisy.

OBJECTIVE:To improve the writing quality prescriptions of traditional Chinese medicines and to facilitate the standardization of traditional Chinese medicine prescriptions.METHODS:A total of 15 000 prescriptions were sampled in our hospital in 2006 for an analysis of the problems in accordance with the related standards specified in Chinese Pharmacopoeia(CP,2005 edition)and the new "Prescription management method".RESULTS:The problems manifested as nonstandard in drug name and footnotes,or overdosage and so on.CONCLUSION:We should strengthen the management of the traditional Chinese drugs and improve our pharmaceutical care.

Objective:To eliminate the bacteriostatic action ofA,wei Capsule in microbial limit examination so as to establishing the method ofmicrobial limit for A,wei Capsule.Methods:The plating and culture medium diluted methods were used to determine the antimicrobial effect ofA,wei Capsule by recovery rates with 5 control trains.Results:According to the results, the recovery rates were higher than 70% by culture medium diluted method, which indicated no antimicrobial effect with them.Conclusion:According to the results, the culture medium diluted method can eliminate the bacteriostatic action ofA,wei Capsule preferably with accurate result and reaching the objective.