Objective To establish a HPLC method for the determnation of serum concentration of isoimperatorin in rats and study pharmacokinetic characteristic of isoimperatorin in rat after drug admiaistraction. Method The concentrations of isoimperatorin in rats were determined after drug administration and the pharmacokinetic parameters of isoimperatorin were estimated by DAS2.0 computer program. Result The concentration-time profiles were fitted with open two compartment model system. The main pharmacokinetic parameters were as follows:Tmax =(1.6?0.000)h, Cmax =(15.842?0.35)mg/L, AUC0-t=(80.761?10.03)mg/(L?h), AUC0-∞=(89.45?9.34)mg/(L?h), t1/2=(5.101?0.24)h. Conclusion The method is appropriate for the determination and pharmacokinetic study of isoimperatorin in rats after drug administration.

Objective To investigate the value of ultrasound in diagnosis of fetal heart malformation at 11-13+6 weeks gestation.Methods A total of 70 pregnant women were selected,based on the results of induction of labor and postnatal imaging examination, the effect of ultrasound on fetal heart abnormalities in 11-13+6 weeks was observed.The display of cardiac malformations with different cuts was compared.Results In 70 fetuses with cardiac malformations, malformation types included tetralogy of Fallot, double outlet right ventricle, complete endocardial cushion defect, transposition of the great arteries, persistent truncus arteriosus, aortic arch, venous drainage.Ultrasound diagnosis of 64 cases of fetal cardiac malformations, the diagnostic accuracy rate was 91.4%.Among them, the highest proportion of tetralogy of Fallot (42.9%), venous drainage was the lowest (2.9%).The diagnostic rate of 4CV+3VVT ultrasound for fetal heart malformation was the highest(91.4%), which was significantly higher than 4CV+VOTV(72.2%), 4CV+(AR-SAV)(68.6%) and 4CV, the differences were statistically significant (x2=7.643,5.324,4.447,all P<0.05).The diagnostic accuracy of 4CV+3VV ultrasound for fetal heart malformation was 88.6%, which was significantly higher than 4CV+ (AR-SAV) and 4CV section, the differences were statistically significant (x2=6.343,4.011,all P<0.05).Conclusion Ultrasound can be used to detect fetal heart malformation at 11-13+6 weeks gestation, among them, the accuracy of 4CV+3VVT ultrasound section is the highest.

AIM: To evaluate the clinical efficacy of surgery for spontaneous subconjunctival orbital fat prolapse via bulbar conjunctiva under topical anesthesia.
●METHODS: A total of 22 eyes of 11 patients received surgery for spontaneous subconjunctival orbital fat prolapse via bulbar conjunctiva under topical anesthesia were included. Objective and subjective duration of the operation, degree of cooperation during surgery, postoperative recovery, recurrence and complications were observed.
●RESULTS: In the process of surgery, patients without pain and being-cooperated were recorded in 17 eyes. The patients who occasionally felt slight pain, but within endurance after adding topical anesthesia once and the operation was completed successfully were recorded in 5 eyes ( the second eye surgery ) . The operation was successfully completed in all the patients. Compared the coordination degree during surgery of Gradel with GradeⅡ, the difference was statistically significant ( t=-3. 123, P<0. 01). All eyes were healed well after operation.
● CONCLUSION: The surgery for spontaneous subconjunctival orbital fat prolapse via bulbar conjunctiva under topical anesthesia can ensure satisfactory anesthetic effect and get better quality of operation. It′s a simple, safe and effective anesthesia method for spontaneous subconjunctival orbital fat prolapse.

Objective To study the effects of metallothionein(MT) egg-milk power on excluding lead in vivo in saturnic rats.Methods A total of 60 Wistar rats were randomly divided into four groups: control(n=20),black control(n=10) and lead-treated groups(n=30).After the rats in lead-treated group were treated with 0.2% lead acetate daily for three weeks,they were randomly divided into two groups: lead-treated group(n=10) and MT egg-milk power treated group(n=20).The rats in the last group were treated with MT egg-milk power for three weeks.At the end of the experiment,samples of whole blood,liver,kidney,brain,femur,faeces and urine were collected for lead detection by atomic absorption spectroscopy.Results As compared with controls,the lead content of samples were significantly elevated in lead-treated rats(P

Objective To evaluate the value of the use of fibrin glue in radical mastectorny for breast cancer. Method 52 patients with breast cancer were divided randomly into two groups. In one group (27 patients) fibrin glue was used during radical mastectomy, while in the other group (25 patients), fibrin glue was not used. The volume of drainage on the first postoperative day and total volume of drainage after the operation were collected and measured. The fluid accumulation underneath the flap was also observed. Result In patients with the use of fibrin glue, the amount of drainage was much less compared with those without the use of fibrin glue. Having marked diversity ( P

ObjectiveTo observe the effect of acupuncture plus language function training on the recovery of language function in patients with post-stroke aphasia.MethodNinety patients were divided into an acupuncture-rehabilitation group (intervened by acupuncture plus language function training) of 30 cases, an acupuncture group (intervened by acupuncture alone) of 30 cases, and a control group (intervened by language function training alone) of 30 cases. The Western Aphasia Battery (WAB) was adopted to evaluate the language function, and also taken as criteria for diagnosis and therapeutic efficacy. The aphasia quotient (AQ), and the 4 basic language functions including spontaneous speech, auditory comprehension, repetition and nomination were observed. ResultAfter intervention, AQ, and scores of spontaneous speech, auditory comprehension, repetition and nomination increased significantly in the three groups (P<0.05,P<0.01). there were significant differences in comparing the scores of spontaneous speech, repetition and nomination between the acupuncture-rehabilitation group and the control group (P<0.05). There were no significant differences in comparing the indexes between the acupuncture group and control group (P>0.05).ConclusionAcupuncture plus language function training can produce a positive effect on patients with post-stroke aphasia, showing therapeutic advantage in improvingspontaneous speech, repetition, and nomination.

Aim To study the immunomodulatory activity of total flavonoids of Litsea Coreana Leveille (LCTF) on cyclophosphamide(CY)-induced immunosuppressive mice. Methods CY (50 mg?kg-1) was administered by intraperitoneal(ip)injection for 2 consecutive days to induce immunosuppressive model. Carbon clearance, quantitative hemolysis and DNFB-induced delayed-type hypersensitivity(DTH) were applied to assay effects of LCTF on nonspecific immunity, humoral immunity and cellular immunity. Results In carbon clearance test, the clearance index (K) and values of phagocytic index (?) were elevated by LCTF (200 and 400 mg?kg-1), indicating the phagocytosis of macrophages was enhanced in immunosuppressive mice.In quantitative hemolysis, productions of IgM and IgG in serum and hemolysin in splenocytes were enhanced in immunosuppressive mice by LCTF (100 and 200 mg?kg-1). LCTF (200 and 400 mg?kg-1) obviously increased DTH reactivity in immunosuppressive mice. LCTF not only increased percentages of T cells expressing CD4+ and CD8+,but also enhanced the ratio of the two subset of T lymphocyte,and LCTF (200 and 400 mg?kg-1) could also improve IL-2 production of spleen lymphocytes. Conclusion LCTF showed significant immunomodulatory property on immunosuppressive mice through specific and nonspecific immunity.

Objective To compare the high risk factors, complications, treatment and prognosis of respiratory distress syndrome (NRDS) in neonates at different gestational age (GA). Methods Between August 2012 and July 2013, 156 neonates with RDS were selected and distributed into 3 groups, 42 early preterm (GA<34weeks), 52 late preterm (GA 35 to 36 weeks), and 62 in term group (GA≥37 weeks). Retrospectively analysis was performed for high risk factors, complications, treatment and prognosis of the three groups. Results In 156 neonates with RDS, the male and female proportion was 2.25:1. All groups had more males, but the gender difference has no statistical signiifcance in three groups (P=0.923). The onset time of RDS and the hospitalization time both show an increasing trend of statistical signiifcance (P<0.05). Comparing the difference of high risk factors for RDS of the 3 groups, birth asphyxia, placental abnormalities, multiple pregnancy, premature rupture of membranes was most common in early preterm group, and followed by late preterm group, and C-section was most common in term group and unexplained preterm was more common in early preterm group than that in late preterm group (all P<0.05). Among the three groups, the ratio of pulmonary surfactant application was the lowest in the term group, the ratio of X-ray grade over II was high-est in early preterm group, oxygen and hospitalization time were the longest in early preterm group (P<0.05). The risks of com-plicated with pulmonary infection, intracranial hemorrhages and bronchopulmonary dysplasia were the highest in early preterm group and the risk of complicated pneumothorax was the highest in term group. Among three groups, the recovery rate was the lowest in the early preterm group (P<0.01). Conclusion The clinical characteristics, high risk factors, complications and treat-ment responses of RDS in neonates with different GA were different, so GA should be considered for diagnose and treatment. For the term infants, the elective caesarean section should be strictly controlled, in order to reduce the incidence of RDS.

Aim To investigate the immunomodulative effect of hesperidin on immunodepressed mice.Methods The immunosuppressed mice were induced by cyclophosphamide (Cy, ip). Indexes of immune organs were calculated. Phagocytosis of mononuclear macrophage was determined by cleaning carbon particle method. Spectrophotography was used to estimate levels of serum specific IgG, IgM (HCIgM, HCIgG). Plaque forming cell (PFC) was determined with quantitative haemolysis of SRBC (QHS). Splenic lymphocytes proliferation was measured by MTT method. The mouse delayed type hypersensitivity (DTH) model induced by dinitoflruorobenzene (DNFB) was used to study the effect of hesperidin on the level of DTH and subset of T lymphocyte. Results Hesperidin remarkably increased indexes of spleen and thymus, the rate of clearance and clearance index, but had no significant impact on HCIgM, HCIgG and PFC. In addition, it could enhance the proliferation of splenic lymphocytes and reverse DTH response to normal level. Conclusion Our results indicated that hesperidin had an enhanced effect on nonspecific immunity and specific cellular immunity in immunodepressed mice, while specific humoral immunity wasn′t significantly changed.

Aim To study the therapeutic effect of hesperidin (HDN) on adjuvant arthritis (AA) in rats and its mechanisms.Methods Freund's complete adjuvant (FCA) was used to induce AA in rats. Secondary paw swelling of AA rats was measured with volume meter. Splenic lymphocyte proliferation response induced by concanavalin A (ConA) or lipopolysaccharide (LPS) was examined with MTT assay. IL-2 production of splenic lymphocytes and IL-1, IL-6,TNF-? productions of peritoneal macrophage (PM?) were determined by radio-immunity assay.IL-10 production of PM? was estimated by enzyme linked immunosorbent assay (ELISA).Results The secondary inflammation of AA rats appeared on the 12th day after injection of FCA. At the same time (d 12), HDN (40,80,160 mg?kg-1,?12 d) were given to AA rats by intragastric administration. It was found that HDN(80, 160 mg?kg-1) could significantly inhibit the secondary paw swelling of AA rats from the 20 th day.The suppressed lymphocyte proliferation and IL-2 production of splenic lymphocytes in AA rats were reversed by treatment with HDN. Meanwhile,HDN could remarkably down-regulate IL-1,IL-6,TNF-? productions of PM? and up-regulate IL-10 production of PM?.Conclusions The results suggested that HDN had therapeutical effect on AA rats. Its mechanisms may be related to adjusting abnormal immune function in AA rats and keeping the balance of cytokine network.