Recent work has revealed that large numbers of mitotic figures are present in adult hearts,which has implied that there should be some mechanism about nature repair of the adult heart.But the levels of natural repair are not high enough to retrieval the traumatic myocardium.As a new approach to improving the cardiac repair,stem-cell therapy provides an exciting and powerful treatment.We describe the background of natural repair,and summarize the current strategies in the application of stem-cell therapy to repair the traumatic myocardium.

Objective To analyze the application of color Doppler ultrasonography in diagnosis of diabetic carotid sclerosis and to study the relation between diabetes and carotid sclerosis. Methods Color Doppler ultrasound findings in 68 hospitalized type 2 diabetes patients ( as an observation group) and in 73 healthy individuals ( as a control group) were compared and analyzed from Feb. 2007 to Feb. 2009. Results The tunica intima and media of carotid were significantly thicker in observation group than in control group ( P

Bardet-Biedl Syndrome ; diagnosis ; therapy ; Child ; Female ; Humans

Objective To explore the characteristics and its influential factors of bone mineral density (BMD) changes in partial androgen deficiency in aging male patients.Methods 186 aging male patients with partial androgen deficiency and 125 healthy persons were selected, and their BMD in the first to fourth lumber spine and the proximal left femur was measured with the dual-energy X BMD measuring instrument.The biochemical and bone metabolic markers and sexual hormones were collected, and the relationship between changes of BMD in the patients with partial androgen deficiency, age, BMI, sexual hormones and bone metabolic markers were observed by multivariate stepwise regression analysis.Results There was no significant difference in BMD at the lumbar spine between two groups( P ＞ 0.05 ), but BMD in PADAM group was lower than control groups at the hip[Neck: (0.831 ±0.136) g/cm2 vs (0.954 ±0.143) g/cm2,War's: (0.712 ± 0.127 ) g/cm2 vs ( 0.811 ± 0.149 ) g/cm2,Troch: ( 0.697 ± 0.124 ) g/cm2 vs ( 0.764 ±0.131 )g/cm2, P ＜ 0.05 )].The incidence of PADAM and control group with osteopenia were 48.9% and 36%, and the osteoporosis was 33.3% and 20.8%, respectively.The bone mineral density was positively correlated to BMI at the first to fourth lumber spine and the proximal left femur in the patients with PADAM and negatively correlated to age and serum level of androgen.Conclusion BMD in PADAM group was significantly decreased, and age and low BMI and androgen deficiency wwere the risk factors of low BMD in PADAM patients.

Carcinoembryonic antigen(CEA)is an oncofetal antigen which has been widespraad studied.A murine stage-Specific embryonic antigen(SSEA-l)is a new oncodeveloptnental antigen which appears at 8-cell stage of mouse embryo.The expression of CEA and SSEA-1 in gastric malignant and nonmalignant tissues by using immunofluorescence technique were reported in this study. Most of gastric carcinoma stained intensively for CEA (68 of 78 cases. 87. 2%) and SSEA-l(89.0%).But CEA only expressed in 15.0% of normal gastric mucosa (3/20) and SSEA-1 in 30.0%. In non-malignant gastric tissues, CEA was expressed in 61.5% (8 of 13 cases)of chronic atrophic gastritis (CAG) and 87.5%(7/8)of chronic superiicial gastritis (CSG)with intestinal metaplasia,but only 38.2% and 29.2% in CSG without intestinal metaplasia and peptic ulcer seperately. SSEA-1 existed in more than 80% of all kind of son-malignant gastric tissues, including CAG, CSG with or without intestinal metaplasia and peptic ulcer. These results indicated that CEA will be of more benefit for the diagnosis of gastric carcinoma or premalignant gastric lesions than that of SSEA-1.

Objective To investigate the effect of erythropoietin (EPO) on the expression of integrin-β1 in hippocampus of neonatal rats after hypoxic-ischemic brain damage (HIBD) and to discuss the neuroprotective mechanisms for EPO.Methods Neonatal Sprague-Dawley rats of 7 days old were randomly divided into 3 groups by random number table method (24 cases in each group):sham-operated group,HIBD group and EPO treated group(EPO group),then each group was further divided into 4 subgroups (n=6) based on different time points following the injection of 9 g/L or EPO (6 h,24 h,3 d,7 d).The expressions of integrin-β1 and integrin-β1 gene in hippocampus were determined by Western blot and real-time fluorescent quantitative PCR(RT-qPCR).Results By Western blot method:integrin-β1 had low expression in the hippocampus of the sham-operated group at each time point,which had no significant difference(0.47±0.22,0.45±0.18,0.54±0.24,0.53±0.22,F=.223,P=0.879).The expression of integrin-β1 in HIBD group was increased at first and then decreased,which had a significant difference(0.62±0.16,1.42±0.32,1.13±0.37,0.81±0.21,F=11.809,P=0.000).In the EPO group the expression of integrin-β1 was rising significantly at 24 h and 7 d and showed two peaks (0.64±0.21,1.10±0.37,0.96±0.30,1.19±0.31,F=3.741,P=0.028).Compared with the sham-operated group at corresponding time points,the integrin-β1 protein expression showed significant increase at 24 h and on 3 d in HIBD group and 24 h,3 d,and 7 d in EPO group (all P<0.05).By RT-qPCR:there was no significant difference in the gene expression of integrin-β1 in ippocampus of the sham-operated group at different time points (0.31±0.13,0.33±0.16,0.34±0.15,0.32±0.17,F=0.036,P=0.990).Gene expression of integrin-β1 in HIBD group was increased at first and then decreased and the summit was at 24 h point(0.72±0.35,1.39±0.33,0.95±0.24,0.61±0.25).The difference between each time point was statistically significant (F=8.022,P=0.001).Integrin-β1 gene expression of EPO group showed two peaks at 24 h and on 7 d(0.77±0.30,1.09±0.27,0.90±0.38,1.17±0.36),and there was a significant difference between 6 h group and 7 d group (P<0.05).Compared with the sham-operated group at corresponding time points,the expression of integrin-β1 gene in between HIBD group and EPO group was extremely higher(all P<0.05).Besides,the gene expression of integrin-β1 in EPO group was higher than that in the HIBD group on 7 d with statistically significant difference (P<0.05).Conclusion Erythropoietin can upregulate the expression of integrin-β1 in the recovery phase of HIBD,which may be one of the beneficial effects of EPO for HIBD.

Objective To investigate the effect of erythropoietin (EPO) on the expression of MMP-2 in hippocampus of neonatal rats after hypoxic-ischemic brain damage (HIBD), and the mechanism of its neuroprotective effect. Methods Neonatal Sprague-Dawley rats of 7 days old were randomly divided into three groups (n?=?48 in each group): sham-operated group, HIBD group and EPO treated group, then each group was further divided into four subgroups (n?=?12) based on different time points following the injection of medication ( 6 h, 24 h, 3 d, 7 d). The expression of MMP-2 in hippocampus was determined by immunohistochemistry and real-time lfuorescent quantitative PCR method. Results Immunohistochemistry: MMP-2 has a small amount of expression in the hippocampus of the sham-operated group, and at each time point, there is no statistically signiifcant difference (P?>?0.05);The expression of MMP-2 in HIBD group and EPO group all show a trend of increase, and peaked at 7 d, the differences between each time point in two groups are statistically signiifcant (P??0.05). Gene expression in HIBD group at 24 h and 7 d points showed twin peaks, and the peak is higher at the 7 d point, but without difference (P?>?0.05). MMP-2 expression of EPO group presents a trend of increase, and differences are signiifcant between each time point (P?

Port-wine stains(PWS)are a common congenital capillary malformation. The lesions of PWS appear to gradually darken in color and thicken with age, and even progress into nodules in late stage. Based on the principles of selective photothermolysis, pulsed dye laser (PDL)has become a standard treatment for PWS. However, for some intractable PWS, single PDL treatment shows unsatisfactory and even no effects. Considering this state, the authors summarize multiple treatment strategies for intractable PWS with thick or nodular lesions, providing a reference for their treatment.

Objective To study the correlation between the blood selenium level and glutathione peroxidase(GSH-Px)with the occurrence of gestational diabetes mellitus(GDM).Methods Among 1 360 pregnant women in our hospital from January 2011 to December 2012,68 cases GDM were diagnosed as GDM (observation group)and other 113 non-GDM pregnant women were ran-domly selected from these 1360 pregnant women as the control group.The serum selenium level and GSH-Px activity were com-pared between the two groups.Results The selenium level and GSH-Px activity in the control group were higher than those in the observation group.The selenium level was (3.07±0.51)mmol/L in the control group and (2.06±0.41)mmol/L in the observa-tion group,respectively;the GSH-Px activity was (197.23±18.73)U in the control group and (151.35±19.67)U in the observa-tion group,the differences between the two groups were statistically significant (P <0.05).Conclusion The decrease of serum se-lenium level and GSH-Px activity may be one of the pathogenic causes of GDM.

AIM: To express the DT389-hbFGF (389 amino acid residues of the N-terminus of diphtheria toxin (human basic fibroblast growth factor) fusion protein for potential targeting therapy towards posterior capsule opacification (PCO) after cataract surgery.METHODS: The DNA of inactivated diphtheria bacillus and RNA of 12-week fetal brain cortex were extracted, respectively. The fragments of truncated diphtheria toxin (containing 389 amino acids of N-terminus, DT389) )and full-length human basic fibroblast growth factor(hbFGF) sequence (encoding 18kDa protein) were amplified by PCR. The two fragments were inserted into pGEX-4T-1 prokaryotic expression vector to obtain pGEX-DT389-hbFGF prokaryotic expression plasmid. After sequence analysis, the expressing plasmid was transformed into Escherichia Coli BL21 strain and expression was induced under IPTG. The expressed fusion protein was purified and identified.RESULTS: The gene fragments encoding DT389 and hbFGF were amplified and their gene sequences were confirmed. Hybrid gene expression plasmid pGEX-DT389 (hbFGF was constructed. The fusion protein DT389-hbFGF was expressed and purified.CONCLUSIONS: The successful cloning and expression of DT389-hbFGF immunotoxin provides a foundation for targeting therapy towards posterior capsule opacification.