Objective To analyze the epidemiological characteristics of public health emergencies and the situation of report and control of public health emergencies in Qijiang District of Chongqing M unicipality during 2009-2013 to provide a scientific ba‐sis for the prevention and control of public health emergencies .Methods The descriptive epidemiology method was used to analyze the directly network reported data of the public health emergencies in Qijiang District during 2009-2013 from the National Emer‐gency Public Health Emergencies Reporting Management System .Results Total 47 public health emergencies were reported in Qi‐jiang District during 2009-2013 ,including 994 cases of caused disease and 1 case of death (food poisoning) .Most of the emergen‐cies were caused by infectious diseases events ,accounting for 82 .78% (39/47) ,food poisoning and other public health emergencies (dog bites) accounted for 2 .13% (1/47) and 14 .89% (7/47)respectively .Respiratory disease events accounted for 78 .72% (37/47) of total infectious diseases events ,the majority were influenza A (H1N1) flu and epidemic parotiditis .The high‐incidence seasons were from April to May(accounting for 34 .04% )and October(40 .42% ) .The most cases happened to the school (especially infec‐tious diseases) ,accounting for 80 .85% of the total events .Conclusion The respiratory infectious disease in the prevailing season is the priority of prevention and control on public health emergencies in Qijiang District .Attentions should be highly paid to the pre‐vention and control of public health emergencies (especially in rural schools);meanwhile ,the dog management and propaganda of prevention and control knowledge should be strengthened to effectively eliminate the potential of hydrophobia .

This paper reported that bronchial artery embolization(BAE)had been used to treat 35 non tumorous patients who suffered from recurrent hemoptysis or acute severe hemoptysis after the given medical treatment was proved uneffective in 35 patients,7 patients had recurrent hemoptysis during 1.5～19 months postoperatively and were treated by BAE again,and there was no recurrence of hemoptysis during 7～34 months follow-up.Therefore, the authors considered that repeated BAE was still effective in treating recurrent hemoptysis.

Objective To summarize the clinical experience of serious hyponatremia resulted from applying pitnitrin to massive hemorrhage of gastrointestinal tract patients.Methods we used pitritrion on 2460 such patients and examined some symptoms of the digestive tract and nerve system,monitoring the dynamic state of blood sodium.Results The re- suits reveal that patients in 30 cases turned out to suffer from serious hyponatremia,whese sodium in the serum appeared less than 110 mmol/L,which was 1.22% of the rest subjects'.The relevant symptoms included nausea,vomit,spoor,coma and twitch etc.Following remedies included liquid supply with a high-concentrated sodium,ceasing using pituitrin,in- take of salute foods,and dynamic monitoring on the state of sodium in the serum.All patients recovered in 1 to 3 days without a single failure.Conclusion Thus we reach this conclusion:When applying pituitrin to massive hemorrhage of gastrointestinal tract patients,the above mentioned symptoms should be closely examined,and blood sodium should be timely monitored so that proper treatment should arrive in time.

Objective To explore the effect of osthole on mast cells and expression of STAT5 gene and protein in them. Methods Passive cutaneous anaphylaxis was made in mice to copy eczema model, then the allergic mast cells were separated, and the ovalbumin was used to induce allergy of mast cells. Different concentrations of osthole were used to intervene the sensitized mast cells.Then the sensitized mast cells were divided into blank control group, osthole high-dose group and low-dose group.At the end of the experiment, morphology of the mast cells was detected by immunofluorescence technology.MTT assay was used to detect the influence of drugs on mast cells proliferation. RT-PCR and Western blotting were used to detect the expression of STAT5 gene and protein. Results As compared with blank control group, the number of mast cells in the osthole groups was significantly reduced, cells and nuclei obviously shrank, even apoptosis of some cells could be observed; the inhibition rate of mast cells in osthole groups was significantly increased in concentration-dependent manner ( P<0. 01 ) . As compared with blank control group (2.16±0.57), gene expression of STAT5 was significantly decreased in osthole high-dose group (0.59±0.12) and low-dose group (0.82±0.13) (P<0.01).The protein expression of STAT5 was also significantly decreased in osthole high-dose group and low-dose group as compared with blank control group (P<0.01). Conclusion Osthole can inhibit the proliferation of sensitized mast cells, and reduce the expression of STAT5 gene and protein.

Objective To discuss the value of amnionic fluid lamellar body count(LBC) as a predictor of fetal lung maturity. Methods Amnionic fluid was obtained during the cesarean section of 41 normal term pregnancies. LBC was performed on each specimen, and lecithin-sphingomyelin(L/S) ratio was detected simultaneously. Results (1)Amnionic fluid LBC was (86?43)?10 3 (72.5?10 3~99.4?10 3) in normal term pregnancy. (2) Positive correlation was shown between the amnionic fluid LBC and L/S ratio(r=0.66,P=0.001). Conclusion The LBC is a rapid and accurate method to determine the fetal lung maturity.

Objective To compare four pretreatment methods of human plasma coagulation factor Ⅷ( FⅧ) before column purification.Methods Cryoprecipitate was dissolved in Tris , before the supernatant was treated with glycine precipitation, PEG precipitation, acid precipitation and aluminum hydroxide gel adsorption , respectively.Activated partial thromboplastin (APTT) was used to measure the activity of the supernatant clotting FⅧ after treatment.The total activity recovery and specific activity of the final samples were used to weigh the efficacy of those methods .The purity of the intend-ed protein was estimated by non-reducing SDS-PAGE electrophoresis .Results Total activity recovery of glycine precipitati-on was the highest (94.00%±7.60%), followed by that of acidic precipitation (89.47%±2.60%) and PEG precipita-tion (80.92%±9.67%) methods.The lowest was aluminum hydroxide gel adsorption (78.65%±7.52%).Glycine precipitation and PEG precipitation could more effectively remove contaminating protein than acid precipitation and aluminum hydroxide gel adsorption .Treated by four different methods , the specific activity of FⅧ of glycine precipitation sample was the highest (0.6856 ±0.1258 IU/mg), followed by PEG precipitation (0.5773 ±0.0787 IU/mg) and acidic precipitation (0.3885 ±0.0301 IU/mg).The specific activity of aluminum hydroxide gel adsorption was the lowest (0.2879 ±0.0472 IU/mg).Conclusion PEG precipitation is more effective for the actual production process than the other three methods .

BACKGROUND: Biomechanical mechanisms are complex, and previous studiers focus on the stress conduction in the carpus. However, the stress distribution and characteristics of trabecula in the carpus are rarely reported.OBJECTIVE: To investigate the stress distribution and deformation characteristics of the normal lunate through a two-dimensional sagittal finite element model.METHODS: A normal cadaveric lunate sample was scanned with Micro-CT and the central sagittal image was chosen for further finite element analysis (FEA). The chosen image was processed and imported into the finite element analysis software (Ansys 14.0). A two-dimensional sagittal finite element model of the lunate bone was established. Axial pressure was applied to the model with the wrist held in different positions, and nine regions of interests (ROIs) were identified, for which stress and displacement nephograms were created. These included the first principal stress (S1, the maximum stress in a principal plane), the third principal stress (S3, the minimal stress in a principal plane), shear stress (SXY, the component of stress coplanar with a material cross section), von Mises stress (SEQV, yielding begins when the elastic energy of distortion reaches a critical value)and displacement of each ROI (UY, displacement on the vertical plane of the lunate) which were calculated and compared.RESULTS AND CONCLUSION: (1) The stresses on ROIs located in the proximal and volar cortices of the lunate bone were much higher than those in the distal and dorsal cortices. At the proximal lunate, S1 was less than S3; however at the distal lunate, S1 was greater than S3. The ROIs of the distal and proximal ends of the lunate bone received much higher stress than the ROIs of the middle part. As for axial trabecular displacement,both distal and proximal ROIs were compressed by axial pressure. However, the dorsal and the volar parts of the proximal lunate moved in different directions at different wrist postures. Besides, the stress values and magnitudes of displacement were elevated in wrist flexion and extension compared to neutral position.Furthermore, the stress concentration zones (the proximal volar ROI, the proximal dorsal ROI, the distal volar ROI, and the distal dorsal ROI) had different directions of shear stress and displacement in different wrist postures. (2) These results suggest that when stress is loaded on a normal lunate model, four stress concentration zones, the proximal volar ROI, the proximal dorsal ROI, the distal volar ROI, and the distal dorsal ROI are found. The wrist postures can significantly affect the value and distribution of axial stress on the sagittal lunate.

Objective　The covered tracheal stent in shape of a straight tube or a fork-like tube was placed clinically to treat tracheal stricture and tracheal fistula caused by malignant tumour in order to maintain the normal function respiration and to keep the fistula closed.Methods　The stent was made of stainless steel in shape of "Z"covered with membrane partially or entirely.An instrument with tube in three sheathes was used to place the stent in position.Results　The tracheal stents were placed successfully in 9 cases at first time and the esophageal stents were placed for 6 cases among them in the meantime.Dyspnea of patiants was remarkably alleviated.The tracheal fistula of them was closed effectivelly.Follow-up for 4～12 monthes all cases demonstrated no adverse effects.Conclusion　The application of the covered stent is a safe effective method to treat the tracheal stricture and tracheal fistula.

Objective To analyze the epidemiological characteristics of unintentional injuries among children patients at age of ≤ 14 years so as to provide scientific basis for prevention of the children injury. Methods ICD-10 coding was used to retrospectively analyze the epidemiological characteristics of the children patients with unintentional injuries at age of ≤ 14 years admitted from January to December 2009 and the factors affecting the prognosis were discussed. Results There were 3011 patients, with 1991 males (66.12%) and 1 020 females (33.88%). The patients at ages of < 1, 1-4, 5-9 and 10-14 years accounted for 8.24%, 53.30%, 27.90% and 10.56% respectively. The patients from the rural and urban areas accounted for 56.16% and 43.84% respectively. Trauma was the leading sort of injuries in four age groups, accounting for 70.56%, 62.68%, 89.64% and 87.74% respectively. Fall from height was the first cause of unintentional injuries in four age groups, with proportion for 47.58%,36.39%, 51.90% and 56.29% respectively. Muhinomial logistic regression analysis revealed that hospital stay, therapeutic mode ( operation/non-operation), inhabitancy ( urban/rural), causes of injuries and injury severity on admission were the risk factors affecting the prognosis (P < 0.05 ). The rural areas caused higher risk of disability than the urban areas ( OR = 2. 170 ,P < 0.01 ) and non-operation produced higher risk of death than operation (OR = 6.352,P < 0. 01 ). Conclusions The male patients with unintentional injury are more than the female ones, with the highest incidence rate in children at age of 1-4years. Trauma is the main kind of unintentional injury and the fall from height the leading cause of injury.The difference of the rural and urban areas indicates that correct choice of the preventive measures based oh the condition of the local area can attain effective prevention and control of the unintentional injuries.

Objective:Since 1996,the esophageal stent of the curved type has been applied clinically by authors in order to solve the defects which the straight tube like stent could not coincide with the shape of the esophageal of the patient.Methods:The curved type stent made of "Z" type stainless steel was implanted after fixing it direction along the selected esophagus.Results:The application of the esophageal stent of the curved type in 29 cases indicated that the stents were coincidence with the folded esophagus,could decreases the pain and discomforts of the patients,and improved the stability of the stents.Conclusion:The esophageal stent of the curved type applied to the folded esophagus is much more effective clinically than the straight tube like type used before. [