OBJECTIVE:To study the interaction between bovine serum albumin(BSA)with lamivudine,efavirenz,tenofovir and its mechanism. METHODS:Fluorescence spectroscopy was used to determine the interaction between BSA with different con-centrations of lamivudine,efavirenz,tenofovir under different temperatures. The fluorescence intensity of them were determined re-spectively;quenching constant(KSV),apparent quenching constant(Kq),binding constant(KA),binding site(n),thermodynamic enthalpy change(ΔH),free energy diversification(ΔG)and entropy change(ΔS)were calculated according to Stern-Volmer equa-tion and so on. Molecular docking model of 3 drugs with BSA was established by using Sybyl 6.7 Flex X model. RESULTS:Kq for the interaction between 3 drugs with BSA were all higher than 2.0×1010 L/(mol·s),and were decreased with the increase of temper-ature;all n were close to 1,and thermodynamic functions ΔG<0,ΔS<0,ΔH<0. Molecular docking model showed that 3 drugs were mainly bound with BSA at Sudlow Ⅰ subdomain site. CONCLUSIONS:There are the interaction between 3 drugs with BSA;fluorescence quenching mainly manifests as static quenching;binding reaction belongs to spontaneous molecular action pro-cess;binding force mainly includes hydrogen bond and Van der Waals'force. The result of fluorescence experiment is consistent with those of molecular docking,and they complement each other.

The character, classification, scoring, diagnosis and treatment of abdominal injuries after the Wenchuan earthquake were analyzed based on the data collected in Chengdu and Dujiangyan. Most of the abdominal injuries were closed injury, and complicated with injuries in other organs. All the injured persons were classified into different groups according to the degree of injuries, so as to determine the optimal sequence of treatment. Open abdominal injuries were comparatively easy to diagnose, while the diagnosis of closed abdominal injuries was difficult and consisted of preoperative diagnosis and intraoperatire exploration. The principle of damage control surgery should be well followed in rescuing the persons with severe abdominal injuries.

Complications of artificial joint replacement include infection,aseptic loosening,prosthesis dislocation and breakage,and deep venous thrombosis. Type 2 diabetes mellitus induces capillary pathological changes,tissue blood supply decrease,leading to general and local resistance reduction. Type 2 diabetes mellitus is always complicated by heart,renal,or cerebrovascular complications,which contributes to high infection rate and frequent complications after artificial joint replacement. In addition,incidence of postoperative pulmonary infection and urinary tract infection is increased,and wound healing is delayed. Deep venous thrombosis is a common complication of diabetic patients following artificial joint replacement,which increases the complexity of perioperative treatment. Blood glucose instability may induce circulation function instability and increase indefinite factors during anesthesia. Therefore,blood glucose control is important to reduce risks in artificial joint replacement for diabetic patients.

In October 2010,the Department of Medical Administration of the Ministry of Healthy of China published Diagnosis and Treatment Standard of Colorectal Cancer.Since then,the diagnosis and treatment of rectal cancer are under regulation.Standardization of preoperative diagnosis and proper selection of imaging or histopathological examinations are key points in improving the efficacy of individual treatment of patients with rectal cancer.In this article,suggestions from the National Comprehensive Cancer Network (2011 version ),American College of Radiology and College of American Pathologists are analyzed,and the recommendations of imaging and histopathological examinations are highlighted.

Objective To explore the clinical manifestation,imaging features and treatment of primary lacri-mal gland epithelial tumor.Methods The clinical data of 25 cases with primary lacrimal gland epithelial tumors were retrospectively studied.Results All of 25 primary lacrimal gland epithelial tumor cases received surgical treatment. Fourteen primary orbital tumors cases were male and 11 cases were female.The mean age was 44 years old (ranged 23 to 65).The mean hospital stay was 12d(ranged 7 to 20).Among 25 primary lacrimal gland epithelial tumor cases, 11 cases were benign tumors which included 4 inflammatory pseudotumor,11 pleomorphic adenoma.Fourteen cases were malignant tumors which included 4 malignant pleomorphic adenoma,6 adenoid cystic carcinoma and 4 adenocar-cinoma.After opeation,visual acuity improved in 9 cases,unchanged 10 cases,decreased 6 cases.The patients were followed up for 16 -48 months(mean 27 months).There were 4 malignant tumors recurrence after operation and received radical operation.While 2 patients were lost and 2 patients died of tumor metastasis,the other 21 patients survived with tumor -free.Conclusion Primary lacrimal gland epithelial tumors have different clinical and imaging appearances.Combination of ultrasound,CT and MRI is important to ascertain the character,range and degree of primary lacrimal gland epithelial tumors.Surgical excision is the main and effective treatment for primary lacrimal gland epithelial tumors,while gamma knife treatment is safe and effective for malignant,unresectable,recurrences tumors.

Hepatectomy ; methods ; Humans ; Laparoscopy

Objective To study the application of ABCD3-I score in predicting the risk of early is-chemic stoke after transient ischemic attack (TIA) .Methods One hundred and eighty-two carotid TIA patients were divided into low risk group (n=40) ,moderate risk group (n=74)and high risk group (n=68) according to their ABCD2 ,ABCD3 and ABCD3-I scores .The incidence of ischemic stroke was observed within 7 days after TIA .Results The area under the ROC curve for ischemic stroke within 7 days after TIA was 0 .625 ,0 .713 and 0 .831 ,respectively .Twenty-seven patients (14 .8% ) developed ischemic stroke within 7 days after TIA .The incidence of ischemic stroke was significantly higher in moderate and high risk groups than in low risk group and in high risk group than in moderate risk group (6 .8% vs 0% ,32 .4% vs 0% ,32 .4% vs 6 .8% ,P< 0 .01) .The ABCD3-I socre was positively related with the incidence of ischemic stroke within 7 days after TIA (r=0 .486 ,P<0 .01) ,suggesting that ABCD3-I socre could significantly affect the incidence of ischemic stroke within 7 days after TIA (P<0 .05) .Conclusion ABCD3-I score can effectively predict the risk of early ischemic stroke after TIA ,and can thus be used in assessment and treat-ment of T IA .

This study was aimed to explore effect of Gui-Zhi Fu-Ling W an (GZFLW) on HIF-1α and vascular en-dothelial growth factor (VEGF) in patients with endometriosis. A total of 68 endometriosis patients in the First Peo-ple's Hospital of Xining from June 2011 to June 2013 were included. All patients received treatment of GZFLW, once a day. After one-month treatment, the treatment efficacy was analyzed. The endometrial tissues (both ectopic endometrium and eutopic endometrium) were obtained before and after treatment. The protein levels of HIF-1α and VEGF as well as the relationship of HIF-1α and VEGF were analyzed by SP immunohistochemistry. The results showed that the total treatment efficacy was 86.76%. Compared with pretreatment, the posttreatment protein levels of HIF-1α and VEGF were significantly reduced (P< 0.05). The decreasing of VEGF protein level and HIF-1α expres-sion level were positively correlated (P< 0.05). It was concluded that GZFLW had better efficacy in the treatment of endometriosis. One of the reasons may be the downregulation of protein levels of HIF-1α and VEGF in endometriosis tissues.

Objective To establish a reversed HPLC method to determine the contents of gatifloxacin and tinidazole simultaneously. Methods ODS C_ 18 column (4.6mm?250mm,5?m) was used as an analytical column. The mobile phase consisted of 15% acetonitrile, 25% of methanol and 60% of 0.05mol/L KH_2PO_4 buffer,which contained 0.5% triethylamine and was adjusted to pH 3.5 by phosphoric acid. The flow rate was 1ml/min. The detection wavelength was 318nm. The column temperature was 30℃. 20?l of diluted sample solution was injected to the sampler. Results The calibration curve of gatifloxacin was linear in the range of 2~20?g /ml (r=0.999 6) ,and that of tinidazole was 2~20 ?g /ml (r=0.999 9). The recovery rates of them were 100.1% and 100.4%,respectively. RSDs of both drugs were less than 1.1 %. Conclusions The method is simple,accurate and sensitive and can be used to determine the two components simultaneously.