1.A retrospective analysis of newborn disease screening in Wenzhou Longwan from 2002-2009
Renlin LIU ; Rong XU ; Mei PAN ; Xiaowei ZHANG
Chinese Journal of Primary Medicine and Pharmacy 2011;18(11):1500-1501
Objective A retrospective analysis of the situation of newborn disease screening in Longwan Wenzhou from 2002 to 2009 was conducted.Methods The restdts of newborn diaease screening of 46 202 neonatus in Longwan district of Wenzhou between 2002 and 2009 were analyzed.The neonatns were screened within 72 hours after birth for congenital hypothyroidism(CH),phenylketonuria(PKU)by measuring thyroid-simulating hormone (TSH)and phenylalanine concentration on dried blood spots on filter paper cards.Neonatus weYe recalled after screened positive and the diagnosis was made by testing serum concentration.Results A total of 46 202 newborns were screened from 2002 to 2009,and 23 cases was positive and the total positive rate was 0.05%,22 cases were confirmed positive for CH.with an incidenee of 1/2 100.1 case was confirmed positive for PKU,with an incidence of 1/46 202.All patients were followed-up by experts of newborn disease screening centre of Zhejiang Province.Conclusion The results of this study confirm that newborn discease sereaning was one of the effective methods of early diagnosis of congenital CH and PKU,which could effectively prevent development low of intelligence or physique,and other organs impairment.
2.Passive Smoking in Beijing Public Places
Yu-Qing LI ; Xiu-Rong LIU ; Mei HAN ;
Chinese Journal of Prevention and Control of Chronic Diseases 2006;0(05):-
0.05).92.7%of the objects knew that passive smoking was harmful.71.9%knew that passive smoking made people suffering from cardiopathy more possibly.74.9%knew that the wife whose husband smoked were easier to catch lung cancer.And 84.4%knew that the child whose parent s smoked more possibly took asthma or respiration disease. The correct rates of the four knowledge points were different among different gender and the degree of education,which was higher in female than in male,and higher in high education degree than in the other(P
3.Antagonism of Bacillus spp.B_1 and B_2 Strains Against Fusarium oxysporum f.sp. pisi
Xiao-Mei LIU ; Xiou-Rong CHEN ; Jin-Ji PU ;
Microbiology 1992;0(03):-
The antagonism of two strains B1 and B2 of Bacillus spp. against pea root rot pathogen Fusarium oxysporum f. sp. pisi was studied. The result of pairing culture showed that B1 and B2 strains of Bacillus spp. had strong antifungal activity to the pathogen. The colonial color of the pathogen changed from gray to white, aerial hyphas increased and entangled into group after treatment with the cell-free fermentational filtrate of B1 or B2. Observation under optical microscope showed that hyphas and spores of the pathogen swelled and distorted with concentrated cytoplasm after treatment, the spores could not germinate or germinated abnormally . A lot of vesicles appeared at the top of the hyphas, and the hyphas stopped growing and broke finally, their cytoplasm spilled from the cell. The cell-free fermentational filtrate of B1 or B2 strains contained 1795.53?g/mL and 1345.93?g/mL protein respectively, from which two antifungal proteins of 103.5 kD (B1 ) and 127.6 kD (B2) were purified.
4.Protection of Mn~(2+) on Injury of human kidney tubular epithelial cell line induced by bentamicin
Zhipeng WANG ; Rong ZHANG ; Li LIU ; Qibing MEI
Chinese Journal of Clinical Pharmacology and Therapeutics 2004;0(12):-
AIM: To evaluate the protection of Mn~(2+) on injury of human kidney tubular epithelial cell line induced by gentamicin, and investigate its principals. METHODS: Gentamicin was used to injury human kidney tubular epithelial cell line (HK-2) to produce model of kidney injury. MTT method was used to measure effect of Mn~(2+) on proliferation of cells after they were injured by gentamicin. The spectrophotometry method was used to observe the change of LDH,NAG and SOD effected by Mn~(2+). Ultrastructure was examined by electron microscopy. RESULTS: Although HK-2 cells were injured, Mn~(2+) promoted cells proliferation, decreased LDH activity and NAG content, increased SOD activity, and alleviated dilation of the mitochondria and crushing of lysosomes. CONCLUSION: Mn~(2+) can protect human kidney tubular epithelial cell line from injury induced by gentamicin. It may be in relation to inhibiting the oxidative injury, lightening dilation of the mitochondria, protecting the integrity of lysosomes and decrease the leakage of enzyme.
5.Effects of rheum tanguticum polysaccharides on ulcerative colitis induced by TNBS in mice
Rong ZHANG ; Zhipeng WANG ; Li LIU ; Qibing MEI
Chinese Journal of Clinical Pharmacology and Therapeutics 2004;0(09):-
AIM: To explore the effects of rheum tanguticum polysaccharides(RTP) on TNBS-induced colitis in mice and its probable mechanisms.METHODS: Mice colitis model was induced by 2,4,6-trinitrobenzene sulfonic acid(TNBS).The mice were randomly divided into 5 groups,normal group,model group,RTP-100,200 and 400 group.The macroscopical and histological changes of the colon were evaluated and the cytokines IL-8,IL-10,TNF-?and IL-4 produced by splenocyts were analyzed with ELISA.RESULTS: Compared with the model group,both symptoms and the lesions of colonic mucosa of RTP-200 and 400 group were slighter on ulcerative colitis induced by TNBS in mice. Furthermore,the expressions of TNF-?and IL-8 in colon tissue of mice with TNBS-induced colitis were higher and the IL-10 and IL-4 were lower than that of normal control(P
6.Therapeutic Effect of Montelukast for Bronchial Asthma Combined with Allergic Rhinitis in Children
rong-jun, LIN ; jun-xing, GAO ; xiao-mei, LIU
Journal of Applied Clinical Pediatrics 2006;0(16):-
Objective To explore the therapeutic effects of Montelukast for bronchial asthma combined with allergic rhinitis(AR) in children.Methods Ninety asthmatic children with AR rhinitis were randomly divided into group A,group B and control group.The children in control group were treated only with Symbicort Turbuhaler.In addition to inhalation of Symbicort Turbuhaler,the children in group A were given Montelukast 5 mg/d orally,while the children in group B were given Budesonide Nasal Spray 64 ?g/d.All the children were observed for 6 months.Daytime and night asthma symptom scores,rhinitis symptom scores,the number of days with or without acute asthma symptoms were recorded every 4 weeks.FEV1%,as one of the lung function parameters,was also recorded in the pre-treatment and 8th week,24th week after the treatment.Results After the treatment,the daytime and night asthma symptom scores and rhinitis symptom scores of group A and group B were significantly reduced(group A:F=3.45,3.35 Pa0.05).However,there was better improvement of FEV1%,more days without symptoms and fewer acute asthma attacks in group A than those in group B (t=3.83,3.76,4.26 Pa
7.1H-NMR based metabonomic approach to evaluate detoxification effect of vinegar-processed Euphorbia kansui.
Yu-Mei LIU ; Rong-Rong HUI ; Cui-Cui HE ; Jin-Ao DUAN ; Jian-Xin LI
China Journal of Chinese Materia Medica 2014;39(2):322-326
Euphorbia kansui (EK) is a toxic herbal drug, and often used after vinegar-processing to reduce its toxicity. In present study, a 1H-NMR based metabonomic approach was used to evaluate the detoxification effect of vinegar-processed EK. The water extracts of EK and VEK were administered orally to male SD rats at doses of 9 g x kg(-1) x d(-1) for 1 week, respectively, and one more week observation was further conducted. The control group was orally given with saline. Histopathological studies of liver samples on the 8th and 15th day were conducted, and the metabolites of rat urine and liver were analysed by 1H-NMR. Histopathological studies of liver samples from EK and VEK treated rats showed no negative impacts. In metabonomic analyses of urines, changes of metabolites indicated liver damages, kidney lesions and imbalance of gut microbes in the second week. VEK-treated rats showed a quite lower toxicity compared with EK-treated ones. The present study revealed that the metabonomic approach might be helpful for the evaluation of toxicity of EK and detoxic effect of VEK.
Acetic Acid
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chemistry
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Animals
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Chemistry, Pharmaceutical
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Drugs, Chinese Herbal
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chemistry
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pharmacokinetics
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toxicity
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Euphorbia
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chemistry
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Inactivation, Metabolic
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Liver
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drug effects
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metabolism
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Magnetic Resonance Spectroscopy
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Male
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Metabolomics
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methods
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Rats
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Rats, Sprague-Dawley
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Urinalysis
8.The application of acridine orange fluorescent staining method in circulating tumor cell
Min LIU ; Fuling MA ; Rong HAN ; Mei LI ; Shufen LIU ; Shumin ZHANG
Tianjin Medical Journal 2015;(7):792-795
Objective To establish the screening platform of circulating tumor cells (CTCs) using acridine orange fluo?rescent (AO-F) dyeing method, and to apply it in the screening of peripheral blood CTCs in patients with kidney cancer. Methods Twenty-seven patients with metastatic renal cell carcinoma was included in this study. Primitive tumor cells and kidney cancer cell line 769-P were cultured with different concentrations of fetal bovine serum. Smears were prepared and observed under fluorescence microscopy. The percentage of AO-F positive staining of 769-P cells under 5 random sights was calculated. The sensitivity of AO-F staining to cells was evaluated. The 5 mL morning fasting venous blood was obtained from 10 subjects with healthy check-up. The 1×106 cell suspension was prepared. The logarithmic phase of renal tumor cells was used to prepare tube containing 500, 200, 100, 50 and 10 tumor cell suspension, which were mixed with 1×106 nucleated cells to establish CTCs model of renal cancer. AO-F staining method was used to detect the expression of AO-F positive cells. The correlation between expression of AO-F positive cells and clinical parameters was analyzed. Results The prima?ry cells and cell line 769-P showed similar bright color and morphological characteristics. The percentage of AO-F positive staining in 769-P cells was 93%±3%under 5 random sights. The recovery rates (%) of four groups (500, 200, 100 and 50 tu?mor cell suspension) were 10.2±3.8, 9.2±2.3, 10.8±2.6 and 10.5±1.9, respectively. There were no significant differences in recovery rates between four groups (P>0.05). The group of 10 tumor cell suspension could find AO-F positive staining cells occasionally. Zero case was positive in controls. Nine of 27 patients were positive and the rate was 33.33%. There were no significant statistical differences in AO-F positive rates between gender, age, tumor size, pathological pattern, Furhman stage, metastasis of lung and presence of tumor (P>0.05). Conclusion It is confirmed that the method of CTCs staining with AO-F, which has high specificity and reproducibility, is feasible to detect CTCs and worthy of being studied. There is a certain reference value to predict tumor recurrence and metastasis.
9.Resistin Binding Peptide Stimulates Basal Insulin Secretion of RINm5F Insulinoma Cells
Yun-min, ZHANG ; Chun-mei, ZHANG ; Xia, CHI ; Feng, LIU ; Li, FEI ; Xiao-qin, PAN ; Mei, GUO ; Yu-hui, NI ; Rong-hua, CHEN ; Xi-rong, GUO
Journal of Applied Clinical Pediatrics 2008;23(11):879-883
Objective A resistin binding peptide (RBP) was selected by phage display in our previous work. Studies had shown that RBP could antagonize the role of resistin on the lipid metabolism and endocrine function of adipose tissue, but whether RBP affects the insulin secretion of pancreatic cells is still unknown. The aim of this study is to assess the effect of RBP on basal insulin secretion in RINm5F insulinoma cells. Methods The cell viability was measured by 3-[4,5-dimethyhhiazol-2-yl]-2,5-diphenyltetra-zolium bromide (MTT) cytotoxicity assay. The supernatants were assayed for insulin content by enzyme linked immunosorbent assay (ELISA). Reverse transcriptase-PCR assay and Western blotting were used to determine the expression of glucose transporter 2 (GLUT2) involved in insulin secretion. Cytosolic Ca2+, the trigger of insulin exocytosis, was analyzed with the fluorescent probe FURA-3/AM. Results RBP did no effect on the cell viability with a concentration of 10-8-10-12mol/L of 2 hours intervention. But it stimulated basal insulin secretion of RINm5F cells, accompanied by up-regulated increased expression of GLUT2 and elevated concentration of cytosolic Ca2+. Conclusion RBP could stimulate basal insulin secretion without affecting the cell viability.
10.Effect of high loading dose of clopidogrel on myocardial microcirculation in patients after primary percutaneous coronary intervention
Mei WANG ; Rong YANG ; Jinming LIU ; Yaling WANG ; Yongjun LI ; Hui ZHANG
Clinical Medicine of China 2010;26(11):1148-1150
Objective To explore the effect of high loading dose of clopidogrel on myocardial microcirculation in patients with ST segment elevation myocardial infarction(STEMI)after primary percutaneous coronary intervention(PCI). Methods Sixty-four STEMI patients were divided into 300 mg clopidogrel group(n =32)and 600 mg clopidogrel group(n = 32)randomly. Myocardial blush grades,sum-ST-segment resolution (sumSTR%),placental growth factor(PIGF),soluble CD40 ligand(sCD40L)and left ventricular ejection fraction (LVEF%)were compared after PCI. Results There was no significant difference between the two groups in basic clinical or angiographic characteristics before PCI(P>0. 05). Compared with the 300 mg clopidogrel group,patients in the 600 mg clopidogrel group had higher percentage of blush grade 3 of infarct related arteris(21.88%vs. 50. 00% ,P <0.05)and higher sumSTR%((70. 90 ±9. 51)% vs.(60. 70 ± 15.06)% ,P <0. 05). There were lower serum PIGF levels(14.37 ± 1.32)ng/L vs.(15. 85 ± 1.71)ng/L,P < 0. 05)and sCD40L levels(4. 93 ±0. 71)μg/L vs.(5.68 ± 0. 77)μg/L,P < 0.05)in patients in the 600 mg clopidogrel group after PCI. Seven days after PCI,the LVEF was significantly increased in the 600 mg clopidogrel group(70.96 ± 9. 51)% vs.(65. 27 ±9. 85)% ,P < 0.05). Conclusions High loading dose of clopidogrel before primary PCI can improve myocardium microcirculation in patients with STEMI.