Weanlimg male rats fed on synthetic diet containing 1% succinylsulfa-thiazole and devoid of vitamin B12 and folic acid for 3-5 weeks, developed syndromes of the vitamins deficiency, i.e. poor food consumption, low growth rate, decrease in white cell count and marked increase in formimi-noglutamic acid excretion.Nevertheless, the others had an intake of vitamin B12 (0.02 ?g/day/-rat) and folic acid (0.5?g/day/rat) for a 36-day period, also showed signs of vitamins deficiency. But when given a daily dose of 0.1 ?g of vitamin B12 and 2.5 ug of folic acid, the rats had a good appetite and grew well, and excreted more formiminoglutamic acid than normal rats, when they were given a loading dose (0.05 g/rat) of histidine. Under the similar feed- ing condition, rat ingesting a daily dose of 0.3 ug of vitamin B12 and 7.5ug of folic acid, showed no obvious syndomes of the vitamins deficiency.After the rats were fed on the same diet and given with vitamin B12 (0.02 ug/day/rat) and folic acid (0.5ug/day/rat) for a 36-day period, the animals developed increased radiosensitivity. After total-body irradiation (700 rad), the food consumption, growth rate and survival rate in this group were lower than those in other groups ingesting individually 0.1 ug of vitamin B12 and 2.5 ug of folic acid or 0.3ug of vitamin B12 and 7.5 ug of folic acid daily.After irradiation, in the group of 0.02 ug of vitamin B12 and 0.5 ug of folic acid or 0.1 ug of vitamin B12 and 2.5 ug of folic acid in daily in-gestion there was a considerable increase in formiminoglutamic acid with a loading test of histidine(0.05 g/rat) than before. The results showed that the radiation intensified vitamins deficiency in rats.

OBJECTIVETo develop a single-tube fluorescent bidirectional PCR method to detect the 609C/T polymorphism of NAD(P)H: quinone oxidoreductase 1 (NQO1) gene.

METHODSTwo primers of NQO1 gene C609T locus were designed. Using these primers, a SYBR Green I fluorescent bidirectional PCR, combined with melting curve analysis of the PCR products, were optimized to differentiate the 609C/T polymorphisms in 191 samples of human genomic DNA. The accuracy of the fluorescent bidirectional PCR was validated by the classical method of PCR-restriction fragment length polymorphism(RFLP) in 62 of these 191 samples.

RESULTSIn the 62 samples, the genotypes determined by the fluorescent bidirectional PCR were 100% consistent with the ones by the PCR-RFLP. The frequencies of genotypes of homozygous wild-type (CC), heterozygous (CT), and homozygous mutant (TT) were 28%, 50%, and 22%, respectively, in the 191 samples.

CONCLUSIONThe single-tube fluorescent bidirectional PCR method established here provides a simple, rapid, accurate and inexpensive assay to determine the 609C/T polymorphism of NQO1 gene. The assay is suitable to detect the single nucleotide polymorphism in large-scale samples.

Adolescent ; Adult ; Base Sequence ; Female ; Humans ; Male ; Middle Aged ; Molecular Sequence Data ; NAD(P)H Dehydrogenase (Quinone) ; genetics ; Polymerase Chain Reaction ; methods ; Polymorphism, Restriction Fragment Length ; Polymorphism, Single Nucleotide ; genetics ; Reproducibility of Results ; Young Adult

OBJECTIVESTo analyze the survival outcomes of the surgery for colorectal cancer with liver metastases (CRCLM), and study the mode of multi-disciplinary team (MDT) for CRCLM.

METHODSThe retrospective analysis was conducted for 38 patients with CRCLM received MDT management and surgical treatment from January 2009 to August 2011. The peri-operative and survival outcomes of MDT and surgery were evaluated.

RESULTSAll the cases met the present criteria of resetability for CRCLM, but only 4 cases (10.5%) met the previous one. Coloproctectomy and hepatectomy were performed in all cases, with 39 colorectal neoplasms and 155 liver lesions removed. One case died of postoperative septic shock. Colorectal and hepatic specific complications were absent in the others patients except one case of biliary leak which was treated with conservative management. Neoadjuvant chemotherapy was arranged in 13 cases. Adjuvant chemotherapy was administered for every patient. After a mean follow-up of (22 ± 10) months according to the finding time of liver metastases, recurrence and metastases were observed in 16 cases and 6 cases died of late-stage cachexia. The 1-, 2- and 3-overall survival rate were 94.4%, 85.3% and 75.8% respectively, and the 1-, 2- and 3-disease-free survival rate were 70.1%, 54.2% and 54.2% respectively.

CONCLUSIONSMDT mode for resectable CRCLM is recommendable. Surgical resection of CRCLM is feasible and safe, which seems to achieve favourable short-middle oncologic outcomes. And long-term survival is expected.

Adult ; Aged ; Chemotherapy, Adjuvant ; Colorectal Neoplasms ; mortality ; pathology ; surgery ; Female ; Follow-Up Studies ; Humans ; Liver Neoplasms ; mortality ; secondary ; Male ; Middle Aged ; Retrospective Studies ; Survival Rate ; Treatment Outcome

Objective: To investigate the efficacy and safety of endoscopic retrograde cholangiopancreatography (ERCP) in the diagnosis and treatment of biliary and pancreatic diseases in children. Methods: The clinical data of 127 children who were treated with ERCP in the First Affiliated Hospital of Nanchang University from January 2007 to July 2021 were analyzed. According to the diseases they suffered from, the children were divided into biliary group and pancreatic group. The operation times, technical success rate, diagnosis, interventions and post-ERCP complications between the groups were compared by t-test or χ² test. The risk factors of post-ERCP pancreatitis (PEP) were analyzed by multivariate Logistic regression. Results: A total of 127 children, including 54 males and 73 females, with a median age of 14 years at first ERCP, were included in this study. ERCP was performed in 181 cases, with a success rate of 98.3% (178/181). In pre-ERCP imaging examination, the positive diagnostic rates of ultrasound, CT and magnetic resonance cholangiopancreatography (MRCP) for biliary and pancreatic diseases were 54.1% (53/98), 56.1% (37/66) and 79.3% (88/111), respectively. MRCP had the highest positive diagnostic rate, and the difference among the three measures was statistically significant (χ²=17.33, P<0.001). The most common indications for ERCP in biliary and pancreatic diseases were choledocholithiasis (77.0%, 107/139) and congenital structural abnormalities of the pancreas (31.0%, 13/42), respectively. After ERCP, the abdominal pain was dramatically relieved and the liver function was significantly improved (all P<0.001). The blood amylase level of the children with pancreatic diseases was significantly lower than that before ERCP (t=7.73, P<0.001). The overall incidence of post-ERCP complications was 12.2% (22/181), of which post-ERCP pancreatitis (PEP) was the most common (7.2%, 13/181). The incidence of PEP was significantly higher in the pancreatic group than in the biliary group (16.7% (7/42) vs. 4.3% (6/139),χ²=7.38, P=0.007). Multivariate Logistic regression analysis showed that young age was the independent risk factor of PEP (OR=0.80, 95%CI 0.67-0.96). Conclusions: MRCP is the first choice for pre-ERCP imaging examination of biliary and pancreatic diseases in children. ERCP can be safely and effectively used in the diagnosis and treatment of biliary and pancreatic diseases in children, with a high success rate and obvious alleviation of symptoms.
Child ; Female ; Male ; Humans ; Adolescent ; Cholangiopancreatography, Endoscopic Retrograde/adverse effects* ; Pancreatic Diseases/surgery* ; Cholangiopancreatography, Magnetic Resonance ; Pancreas/surgery* ; Pancreatitis/etiology*