ObjectiveTo explore the mechanisms of proliferation and apoptosis of K562 cells induced by cytarabine. MethodsDifferent doses of cytarabine on the proliferotion at K562 cell 24h,48h,72h were detected by MTT. Cell cycle And Apoptosis rate were detected by FCM. ResultsEach concentration of cytarabine obvious inhibited the growth of K562 cells. In the same concentrations,function of proliferation inhibition with cytarabine increased by the extension of time,which showed dose and time effect relations. Cytarabine on K562 cell,G0/G1 phase cell proportion of role as cytarabine increased when extension of time, S period and the G2/M period cells were decreased.Apoptosis of K562 cell in proportion as drug concentration and effect time increases, which present Cytarabine timeconcentration dependence. CondusionCytarabine was apparent inhibition to proliferation of K562 cell leukemia cell, which could promote K562 cell apoptosis by time and concentration dependence way.

0.05) and associated with histopathologic grade and clinical stages (P

Objective:To explore clinical significance of the further method dealt with in ASCUS(atypical squamous cells undetermined significance) of cervical smear.Methods:118 patients with ASCUS were detected by colposcopy and high risk HPV test,simultaneously make biopsy of cervical guided by colposcopy.Result:By diagnosed with pathology,chronic cervical inflammation is 72 cases,46 cases were CIN(cervical intraepithelial neoplasia).In this study 40 cases were positive in HPV test which 33 cases are CIN(?2=48.18,P0.05).Conclusion:There are high CIN csses in Women with ASCUS whose should be paid attention to and strengthen management.Colposcopy and high risk HPV test are efficiency method to ASCUS,especially high risk HPV test is positive that need detective by colposcopy.The combination of colposcopy and high risk HPV test can improve detective rate and accuracy of cervical intraepithelial neoplasia(CIN).

Type 2 diabetes is associated with a marked increased risk of cardiovascular disease (CVD).Treatment for dyslipidemia in diabetes reduces cardiovascular event.This article reviewed the clinical trials of lipid lowering in diabetic subjects,treatment goals for lipoprotein therapy,and how to choose lipid lowering agents.

OBJECTIVE: To summarize research progress of tissue-engineered tendon stent material and its application for repairing tendon defect. METHODS: A computer-based online search was conducted with the key words of "tissue engineering, tendon, scaffold" in both Chinese and English from January 1993 to October 2009. Articles about dynamical performance of tissue-engineered tendon,experimental research and clinical application of tissue-engineered tendon were included, but duplicated studies and Meta analysis were excluded. Among 38 articles, 4 major viewpoints were discussed: research progress of tendon tissue engineering; biodynamical analysis of tissue-engineered tendon stent materials; application of biomaterials for tendon tissue engineering; clinical application of tissue engineering for repairing tendon defect. RESULTS: Natural polymer and synthetic macromolecule were major materials in tendon tissue engineering. The preparation and selection of stent materials depended on producing imitated materials with advanced performance and structure based on artificial synthetic materials, preparing natural derivative stent materials based on natural extracellular matrix, and constructing tissue-engineered tendon based on original bio-structure and bio-performance of biological tissue. Construction of tissue-engineered tendon must simulate three-dimensional physical environment in vivo. Microcomputer-controlled step motor was used to force tendon-matrix compound stent in order to increase proliferation velocity of tendon cells and secretory volume of collagen and develop into stress direction. CONCLUSION: Physical stimulation is simple, thus technique and instrument for constructing artificial tendon need to be further studied. Additionally, living implants which may completely replace body tissue and organ function still need to be further studied and solved.

BACKGROUND:Tumor stem cels have self-renewal, drug resistance and metastasis tumorigenicity, which play an important role in occurrence, development and metastasis of tumors. Currently, there are two methods to identify tumor stem cels, namely, in vitro tumor sphere culture experiments and in vivo mouse tumorigenic experiments. However, there ia a lack of reports regarding clinicaly enriched gastric cancer cels by chemotherapy. OBJECTIVE:To investigate the enrichment of rat gastric cancer stem cels by cisplatin, and to explore the screening methods for their surface marker proteins. METHODS: BCG-823 gastric cancer model was established in rats, and then rat models were randomized into two groups: rats in experimental groups were subjected to intravenous injection of 0.1, 0.2, 0.25, 0.3 g/L cisplatin via the tail vein; those in control group were injected with normal salinevia the tail vein. After three courses of chemotherapy, gastric stem cels-enriched tissues were colected. Tumor surface proteins were extracted using high-throughput protein microarray and identified by western blot assay. Effects of cisplatin on enrichment of rat gastric cancer stem cels and screening methods for surface marker proteins were compared. RESULTS AND CONCLUSION:Cisplatin at a dose of 0.3 g/L×200μL exhibited the best therapeutic effects, and moreover, with the dose increasing, the tolerance became worse and the incidence of adverse reaction became higher. Transplantation tumors were verified by hematoxylin-eosin staining. Western blot test results were similar to the findings of protein microarray method, that is, HLA-DQ, PMP22 and Claudin7 protein expressions increased in gastric tissues, but HLA-DR, CD14, CD16 and CD56 protein expression decreased. These findings suggest that cisplatin can be used to enrich gastric cancer stem cels in rats, and to successfuly screen the corresponding surface marker proteins.

Objective To explore the factors related with osteoporosis in middle-aged men who accepted high education. Methods 306 men were investigated about their bone density, also their behaviors, diet, exercise and so on. Results The incidence of osteoporosis increased with the aging. The Logistic regression showed that smoking (OR=4.61) and alcohol intake (OR=1.11) were the risk factors for osteoporosis,while the milk intake (OR=0.19) and exercise (OR=0.13) were the protective factors. Conclusion The low bone mass and osteoporosis of middle-aged men accepted high education are associated with the age, smoking, alcohol or milk intake and exercise.

Objective To establish matched-pairs of DNA samples with copy-number controlled differential target genes, and to compare the detection sensitivity of typical Representational Difference Analysis (RDA) method and Subtractive Hybridization Difference Display (SHDD) method in isolating differential genes.Methods Two gene fragments (376 bp and 869 bp in length respectively) cloned by PCR using Human Papilloma Virus (HPV) DNA as template were used as differential target genes, and mixed with human genome DNA. Five matched, pairs of human genome DNA samples with gradually increased difference in copy numbers of target genes were established and RDA and SHDD methods were performed to clone differential target genes and compared their detection sensitivity. Results By using RDA method, 376 bp fragment with 6-fold difference and 869 bp fragment with 8-fold difference were cloned.However, both of these two target fragments with 4-fold difference were isolated using SHDD method.Conclusion The SHDD method adopts balanced bi-directional subtractive hybridization between two sample difference products and avoids loss of differential target genes caused by unbalanced subtractive hybridization of RDA method, and thus outweighs RDA method in isolating target genes, especially long-length target fragments, with small difference.

Objective To explore the feasibility of two-day laparoscopic cholecysteetomy(LC).Methods The clinical data of 50 patients were analyzed retrospectively.Results All the operations were successful.Condu-sions Two-day laparoscopic cholecystectomy is deserved to spresd which is also safe,available and higher bed rota-tion as well as great patient satisfaction.

With the development of funcgonal laboratory teaching,the advantages of integrating laboratory resources have been embodied in someway,improving the students'experiment level and their comprehensive analysis ability.But many new problems have also shown up such as unreasonable curriculum,construction of teaching staff.By reinstalling experiment courses,integrating the experiment teaching staff,and exploring the solution to the existing problems,the teaching quality of functional experiment can be promoted