Objective To investigate the application value of determining the levels of biochemical indexes of bone metabolism in the early diagnosis of middle and old age osteoporosis.Methods 241 middle and old aged individuals undergoing the physical exami-nation in the health management center of our hospital from October 2012 to October 2013 were selected,determined the bone min-eral density(BMD)of the lumbar spine and divided into the normal bone mass group(n=66),osteopenia group(n=58),osteoporosis group(n= 63 )and severe osteoporosis group(n =54)according to the different BMD.The serum levels of bone-specific alkaline phosphatase(B-ALP),insulin-like growth factor-1(IGF-1)and tartrate-resistant acid phosphatase 5b(TRACP5b)were detected.The detection values of biochemical markers of bone metabolism in the four groups were statistically analyzed.Results There were sta-tistically significant differences in the serum levels of TRACP5b,B-ALP and IGF-1 among the four groups(P <0.05).The serum levels of TRACP5b,B-ALP and IGF-1 also had statistically significant differences between the osteopenia group,osteoporosis group and severe osteoporosis group with the normal bone mass group too(P <0.05);the serum levels of TRACP5b and B-ALP were in-creased with the decrease of bone mass(P <0.05),while the serum level of IGF-1 was decreased with the decrease of bone mass(P<0.05).Conclusion Detecting the serum levels of TRACP5b,B-ALP and IGF-1 is conducive to the early diagnosis of middle and old age primary osteoporosis.

Objective To observe the clinical efficacy of thrombolytic therapy combined with psychological intervention in treatment of patients with acute myocardial infarction.Methods 80 cases were treated in department of emergency, Hangzhou First People’s hospital were randomly divided into observation group and control group from February 2014 to February 2015.40 cases in each group.Observation group was given psychological intervention conventional therapy plus thrombolytic therapy, control group were treated with thrombolytic therapy alone, recorded and analysed of therapeutic effect of two groups of patients.Results NOS (nitric oxide synthase), ET (endothelin) values of observation group post-treatment were (45.9 ±5.1)U/mL,(87.3 ±7.1) pg/mL,which were better than control group (39.1 ±3.6)U/mL,(96.5 ±9.3)pg/mL (P<0.05); observation group total effective rate was 75.0% better than control group ( 70.0%) , but the two groups had no significant difference in efficiency.Conclusion Thrombolytic therapy combined with psychological intervention in the treatment of acute myocardial infarction is effective , precise, and can be effectively improve the symptoms of acute myocardial infarction, it is worthy of further research and application.

OBJECTIVE:To evaluate the status quo and the tendency of antiepileptic drugs used in6Children's Hospitals in the Yangtze Valley.METHODS:Data on drug variety,sales volume,manufacturers of antiepileptic drugs used in6Chil?dren's Hospitals in the Yangtze Valley during2002～2004were analyzed statistically.RESULTS:Sodium valproate and topi?ramate took a bigger share in the market with the proportion of both to the antiepileptic drugs in sales volume at about40%.The old variety still took the lead in the clinical drug consumption while the momentum for the consumption of new one was quite fine.CONCLUSION:There are only a few varieties of antiepileptics now yet the sales volume of which is on the rise,therefore,of which there is still prospect of development.

Objective:To explore the effect Yizhiningshen Granules on recognition function of animal model with attention deficit hyperactivity disorder(ADHD).Methods:The treatment groups were given the high,middle and low dosage of Yishi-ningshen granules respectively,Jingling liquid was given to control group of TCM and Ritalin to control group of Eestern medicine.Recognition function was tested by Morrris Water Maze(MWM) method.Results:In model group,the course of learning delayed and the ability of learning acquisition and memory retaining decreased obviously in MWM test.Among five treated groups,compared with the Ritalin group,the course of learning decreased and the ability of learning acquisition and memory retaining improved markedly in the TCM groups(P

Objective:To evaluate the diagnostic value of CT in thyroid benign and malignant tumors.Methods: Thirty one patients with thyroid tumor received CT scan. Results:On the axial images,calcification,hemorrhage,wall nodule,metastatic lymphadenopathy and tumor reinforcement levels after enhancement were observed in thyroid tumors.Among the 31 cases,thyroid carcinoma was diagnosed by CT in 18 cases and thyroid benign tumor in 13 cases,compared with pathologic diagnosis,the coincidence rate was 94.7% and 92.3% respectively. Conclusion: CT scan is very important in the differential diagnosis between benign and malignant thyroid tumors,especially in the correct diagnosis of thyroid carcinoma.

China ; epidemiology ; Hong Kong ; epidemiology ; Humans ; Microscopy, Electron ; SARS Virus ; genetics ; growth & development ; ultrastructure ; Severe Acute Respiratory Syndrome ; epidemiology ; prevention & control ; virology

OBJECTIVE:To promote the informationization and standardization of narcotic drug management so as to enhance the level of pharmacy management. METHODS:The function of intelligent narcotic drug management cabinet and its application in our hospital were introduced. The application effects were evaluated by comparing the indexes in 1 month(or 1 quarter)before and after the adoption of the cabinet. RESULTS:The intelligent narcotic drug management cabinet used in our hospital had such func-tions as login,getting drugs,adding drugs,expiration date management,inventory query,update,shifting of duty,record and da-ta processing. After the adoption,the inventory decreased by 35.2%,turnover rate increased by 53.4%;the time of applying for and registering narcotic drugs by the keeper of secondary warehouse respectively shortened by 84.1%(13.2 min vs. 2.1 min) and 88.9%(10.8 min vs. 1.2 min);and the incidence of external errors reduced from 3 times to once,an decrease of 66.7%. Mean-while,high integration of space improved the overall image of the pharmacy and the automatic pharmacy was constructed on a cer-tain scale and in a systematic manner. CONCLUSIONS:The application of intelligent narcotic drug management cabinet to the out-patient pharmacy can reduce the inventory cost,increase work efficiency and the safety of drug use,and make the narcotic drug management more standard,scientific and sophisticated.

OBJECTIVE To evaluate the effect of azithromycin on mycoplasmal pneumonia(MP). METHODS We divided 204 MP cases into treatment and control groups.In treatment group 10mg/(kg?d) ivgtt for 3-5 d,while in control group 30mg/(kg?d) erythromycin dripping iv.qd for 7-10 d and then observe the effect. RESULTS In treatment and controll groups the effective rate was 91.5% and 78%,respectively.The average days of curing for cough were 6.8 days and 8.7 days(P

Acute Disease ; Coma ; chemically induced ; Female ; Humans ; Middle Aged ; Organophosphate Poisoning ; Pesticides ; poisoning

Objective To investigate the effects of caveolin-1 overexpressing on the growth of cervical squamous cell cancer Hela cell line. Methods Eukaryotic expression vector of human caveolin-1 gene was introduced into Hela cells by Lipofectamine. The clones stably overexpressed caveolin-1 were identiffed by RT-PCR, immunofluorescence cell staining techniques and Westernblotting. Cells proliferation viabihty was tested by MTT assay, and flow cytometry was used to assay the cell cycle and apoptosis, and the relative phosphorylation level of extracellular regulated protein kinases (Erk1/2) were detected by Westernblotting. Results The clones stably overexpressed caveolin-1 were obtained. Compared with the parental Hela cells, the tranfected cells exhibited a slower rate of growth. FAGS analysis results revealed that overexpression of caveolin-1 resulted in the cell cycle arrest in the G0/G1 [ ( 68. 04 ± 2. 57 ) % vs ( 53.41 ±1.01)%] phase and increased the apoptotic cell fraction[ (19. 18 ±2.20)% vs (5.63 ±0.55)%, P <0. 05 ]. Western blotting results showed that overexpression of caveolin-1 reduced the phosphorylation of Erk1/2(0.28 ±0.05 vs 0.81 ±0.07, P <0.05). Conclusions Overexpression of caveolin-1 suppressed the growth of Hela cells and induced apoptosis, down-regulation of Erk1/2 phosphorylation might be involved in its mechanism.