0.05); the effective rates in rest pain in the two groups were 41.25% and 57.97%,respectively(P

OBJECTIVE:To establish an RP-HPLC method for the determination of Berberine hydrochloride in Keyang-min tincture.METHODS:The sample separation was performed on Agilent Eclipse XDB C18(250 mm?4.6 mm,5 ?m),and the mobile phase was acetonitrile-5 mmol?L-1 perfluorooctane sulfonate(32∶68) at flow rate of 1 mL?min-1.The detection wavelength was 346 nm and the temperature of column was 35 ℃.RESULTS:The linear range of Berberine hydrochloride was 0.059 2～0.888 3 ?g(r=0.999 9).The average recovery rate was 99.71%(RSD=1.18%,n=6).CONCLUSION:The met-hod is sensitive,accurate,and reproducible,and suitable for the determination of Berberine hydrochloride in Keyangmin tincture.

OBJECTIVE:To study the effect of Hordeum vulgare on hormone level in male mice.METHODS:Male mature mice(5～6 months) were treated with Hordeum vulgare decoction 1 mL(equivalent to 0.5 g?mL-1 crude drug) bid for 14 consecutive days.Follicle-stimulating hormone(FSH),luteinizing hormone(LH),estradiol(E2) and testosterone(T) in blood were monitored.RESULTS:In Hordeum vulgare group compared normal control,serum E2 increased significantly,serum level of testosterone decreased significantly,while FSH and LH levels remained stable.CONCLUSION:Hordeum vulgare can influence the hormone level in male mice by influencing the gonad axis of the mice.

Objective To explore the clinical manifestation,imaging features and treatment of primary lacri-mal gland epithelial tumor.Methods The clinical data of 25 cases with primary lacrimal gland epithelial tumors were retrospectively studied.Results All of 25 primary lacrimal gland epithelial tumor cases received surgical treatment. Fourteen primary orbital tumors cases were male and 11 cases were female.The mean age was 44 years old (ranged 23 to 65).The mean hospital stay was 12d(ranged 7 to 20).Among 25 primary lacrimal gland epithelial tumor cases, 11 cases were benign tumors which included 4 inflammatory pseudotumor,11 pleomorphic adenoma.Fourteen cases were malignant tumors which included 4 malignant pleomorphic adenoma,6 adenoid cystic carcinoma and 4 adenocar-cinoma.After opeation,visual acuity improved in 9 cases,unchanged 10 cases,decreased 6 cases.The patients were followed up for 16 -48 months(mean 27 months).There were 4 malignant tumors recurrence after operation and received radical operation.While 2 patients were lost and 2 patients died of tumor metastasis,the other 21 patients survived with tumor -free.Conclusion Primary lacrimal gland epithelial tumors have different clinical and imaging appearances.Combination of ultrasound,CT and MRI is important to ascertain the character,range and degree of primary lacrimal gland epithelial tumors.Surgical excision is the main and effective treatment for primary lacrimal gland epithelial tumors,while gamma knife treatment is safe and effective for malignant,unresectable,recurrences tumors.

Objective To study the influence of pine-seed oil upon body weight,blood lipid and lipid peroxidation of mice fed on high fat feed.Method The mice were fed by high fat feed(80% basal feed added 20% lard,HF) and HF added pine-seed oil(80% high fat feed added 20% pine-seed oil,SHF).At 45 d and 90 d,the body fat content,blood lipid level,and malonyldehyde(MDA),lipofusin(LP),catalase(CAT),superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) of brain and liver homogenate were measured respectively.Results Pine-seed oil could reduce the body weight serum triglyceride(TG),total cholesterol(TC),low density lipoprotein-cholesterol(LDL-C) content,but increase high density lipoprotein-cholesterol(HDL-C) of the mice fed by HF.It suggested that pine-seed oil had definite effect on regulating blood lipid.Compared with normal control(NC) group,the activities of CAT、T-SOD、GSH-Px of brain and liver in HF group were remarkably lower,but MDA and LP remarkably higher.It suggested that HF could promote lipid peroxidation in brain and liver of mice.Compared with HF group,the activites of CAT,T-SOD and GSH-Px of brain and liver in SHF group were remarkably higher,but MDA remarkably lower.Conclusion Pine-seed oil can reduce the body weight,blood lipid,and lipid peroxidation of mice fed by HF.

OBJECTIVE:To explore the reason for the "tragedy of anti-commons" in biomedical patent and its effect on the development of biomedical industry.METHODS:The mathematical models of "commons" and "anti-commons" gambling were established based on the analysis on emergence of "patent bush" resulted from the abuse of biomedical patents,and the profit conflict of the patents power related to elementary researcher and applicant researcher was analyzed.RESULTS:The potential "tragedy of anti-commons" affects the enthusiasm of the biomedical researchers,hinders the rapid development of biomedical industry and the improvement of technological level.CONCLUSIONS:The corresponding measures should be taken from aspects such as system,research model,and patent applicant model to balance the possessiveness and exclusiveness,avoid the "tragedy of anti-commons" and facilitate the development of biomedical industry.

OBJECTIVE: To study the bioequivalence of domestic oxcarbazepine dispersible tablets. METHODS: 20 healthy male volunteers were randomly given oxcarbazepine dispersible tablets (test tablet) and oxcarbazepine tablets (reference tablet) via p.o. The plasma concentration of active metabolite (MHD) of oxcarbazepine were determined by HPLC. 3p97 software was used to calculate pharmacokinetic parameters and the bioequivalence of oxcarbazepine were evaluated. RESULTS: After treatment main pharmacokinetic parameters of test tablet vs. reference tablet were as follows: Cmax(4.231?0.841) ?g?mL-1 vs. (4.350?0.861) ?g?mL-1; tmax(4.13?0.43) h vs. (4.28?0.44) h; t1/2(14.17?2.66) h vs. (14.44?2.09) h; AUC0～60 (113.00?22.25) mg?h?L-1 vs. (118.11?14.20) mg?h?L-1; AUC0～∞(126.35?20.94) mg?h?L-1 vs. (130.54?16.17) mg?h?L-1. The relative bioavailability of test tablet to reference tablet was (95.67?12.80)%. CONCLUSION: Two kinds of tablets were bioequivalent.

OBJECTIVE To study the staphylococcus infection and the drug resistance among the outpetients and the inpatients, and to provide information for the clinical treatment.METHODS To cultivate and isolate bacteria according to the Clinical Laboratory Operation Regulation.Bacteria were identified with the automated analyzer VITEK-60.made by Bio-Merieux Company in France.The drug susceptibility was one with the method of single split agar diffusion.RESULTS Totally 159 strains of Staphylococcus were isolated from the outpatients,(46.1%) of which were MRSA.Most of the infection were caused through genital tract;390 strains of(Staphylococcus) were(isolated) from the inpatients,87.1% of which were MRSA.Most of the infection were caused through respiratory tract.Most of MSSA resisted to penicillin,but MRSA were sensitive to vancomycin and(teicoplanin.) (CONCLUSIONS) Preventing the spread of the MRSA is very important to the control of the nosocomial(infection).As far as the infective situation,most of the outpatients are infected through genital tract;while most of the inpatients are infected through respiratory tract.As far as the drug susceptibility,vancomycin is the best antibiotics,the next is teicoplanin.It is necessary to use combined drugs for the MRSA infection.

Objective To find out the status of nosocomial infection management in hemodialysis sections, in order to encourage their continuous quality improvement and standardize their management Methods 41 hemodialysis sections with (≥) 3 dialyzers and independent operation were chosen for investigation. On-site investigation and environmental microbiology monitoring combined. Results The higher the level of the hospital, the better the nosocomial infection control in its implementation of the infection control measures. In terms of rules and regulations, incompliant secondary hospitals and private hospitals accounted for 56. 2% of hospitals of the same ranking; in terms of layout and processes, 75% are found incompliant; and various defects are found in the dialyzer reuse management and disinfection in tertiary and lower hospitals. As to hand hygiene of the staff, the incompliance rate is over 55%. As indicated in the results of microbial environment monitoring, the compliance rate of dialysis water is over 90%, that of dialysate is 78.6% ～ 86.2%, and that of hand disinfection is 56.6%～78.6%.Conclusion It is imperative to strengthen the supervision of secondary hospitals and private hospitals,and to train their staff in the knowledge of hand hygiene and nosocomial infection in hemodialysis rooms.Hospitals found with poor infection management will be encouraged to take issue-specific actions for rectification.