No abstract available.
Broad Ligament* ; Endometrial Stromal Tumors* ; Female ; Neoplasm Metastasis* ; Uterus*

No abstract available.
Female ; Ovary*

This study was conducted to compare the multiple low-dose streptozotocin (MLDS)-induced diabetic patterns of Korl:ICR, A:ICR, and B:ICR mice obtained from three different sources. Six-week-old male ICR mice were obtained from three difference sources. Korl:ICR mice were kindly provided by the National Institute of Food and Drug Safety Evaluation (NIFDS). The other two groups of ICR mice were purchased from different vendors located in the United States (A:ICR) and Japan (B:ICR). All ICR mice that received MLDS exhibited overt diabetic symptoms throughout the study, and the incidence and development of diabetes mellitus were similar among the three ICR groups. The diabetic mice exhibited hyperglycemia, loss of body weight gain, decreased plasma insulin levels, impaired glucose tolerance, decreased number of insulin-positive cells, and decreased size of β-cells in the pancreas. The diabetes symptoms increased as the blood glucose level increased in the three ICR groups. In particular, the level of blood glucose in the STZ-treated group was higher in Korl:ICR and A:ICR mice than in B:ICR mice. The plasma insulin levels, glucose tolerance, blood chemistry, and morphological appearance of the pancreas were very similar in the ICR groups obtained from the three different sources. In conclusion, our results suggest that Korl:ICR, A:ICR, and B:ICR mice from different sources had similar overall responses to multiple low-dose STZ to induce diabetes mellitus.
Animals ; Blood Glucose ; Body Weight ; Chemistry ; Commerce ; Diabetes Mellitus* ; Glucose ; Humans ; Hyperglycemia ; Incidence ; Insulin ; Japan ; Male ; Mice ; Mice, Inbred ICR* ; Pancreas ; Plasma ; Streptozocin ; United States

BACKGROUND/AIMS: The shortage of cadaveric liver donor is particularly critical for children. Split liver transplantation, not only overcomes the drawbacks of reduced size grafts and living donor transplantation for children but also increases the total number of donor organs. The purpose of this study is to examine the technical feasibility of split liver transplantation in pig. METHODS: Nine pigs(3 donors and 6 recipients weighing 19-33Kg) were used. In donor pigs, the liver was divided between right medial lobe and left medial lobe without inflow occlusion under general anesthesia. Left liver was harvested first and then right liver was harvested as usual manner. One recipient pig underwent left lobectomy and left graft were transplanted orthotopically (auxiliary partial orthotopic liver transplantation(APOLT)). For right graft, conventional orthotopic liver transplantation were done. Veno-venous bypass was not performed. Instead, superior and inferior mesenteric arteries were clamped temporarily. RESULT: There was no anhepatic time when using left graft. Cold ischemic time were 2hr 35min, 1hr 21min, and 1hr 5min, respectively. When using right graft, anhepatic time was 72min, 54min, and 49min, respectively. Cold ischemic time was 5hr 17min, 6hr 32min, and 4hr 18min, respectively. Biochemical laboratory data(WBC, hemoglobin, platelet, ALT/AST, LDH, prothrombin time) after reperfusion 1 hour showed good graft function in all transplant pigs and were better in the recipient pigs taking left graft than right graft. Histologic findings at 4 hours after reperfusion show normal appearance except mild ischemic change around central vein. All transplant pigs survived over 24 hours without any major complication. CONCLUSION: APOLT for left graft and conventional liver transplantation for right graft without venovenous bypass were successful in pig. In situ split liver transplantation in pig is technically feasible procedure and this model is suitable for future studies of split liver transplantation.
Anesthesia, General ; Blood Platelets ; Cadaver ; Child ; Cold Ischemia ; Humans ; Liver Transplantation* ; Liver* ; Living Donors ; Mesenteric Artery, Inferior ; Prothrombin ; Reperfusion ; Swine* ; Tissue Donors ; Transplants ; Veins

This study was conducted to compare the anesthetic effects of 2,2,2-tribromoethanol (TBE, Avertin®) in ICR mice obtained from three different sources. TBE (2.5%) was intraperitoneally injected at three doses: high-dose group (500 mg/kg), intermediate-dose group (250 mg/kg), and low-dose group (125 mg/kg). Anesthesia time, recovery time, end-tidal peak CO2 (ETCO₂), mean arterial blood pressure, heart rate, oxygen saturation (SpO₂), body temperature, pH, PCO₂, and PO₂ of the arterial blood were measured. Stable anesthesia was induced by all doses of TBE and the anesthesia time was maintained exhibited dose dependency. No significant differences in anesthetic duration were found among the three different strains. However, the anesthesia time was longer in female than in male mice, and the duration of anesthesia was significantly longer in female than in male mice in the high-dose group. The recovery time was significantly longer for female than male mice in the intermediate- and high-dose groups. In the ICR strains tested, there were no significant differences in the mean arterial blood pressure, SPO₂, arterial blood PCO₂, and PO₂, which decreased after TBE anesthesia, or in heart rate and ETCO₂, which increased after TBE anesthesia. In addition, body temperature, blood biochemical markers, and histopathological changes of the liver, kidney, and lung were not significantly changed by TBE anesthesia. These results suggested that ICR mice from different sources exhibited similar overall responses to a single exposure to TBE anesthesia. In conclusion, TBE is a useful drug that can induce similar anesthetic effects in three different strains of ICR mice.
Anesthesia ; Anesthetics* ; Animals ; Arterial Pressure ; Biomarkers ; Body Temperature ; Female ; Heart Rate ; Humans ; Hydrogen-Ion Concentration ; Kidney ; Liver ; Lung ; Male ; Mice ; Mice, Inbred ICR* ; Oxygen ; Sex Characteristics